Kyle Miller, Sarah Carpinelli, Maxwell Rubin, John Ahlert, Aaron Kubinski, Brendan Van Iten, Kendra Sy, Bryan Lunt, Katerina Meassick, Stephen Ames, Emma Smith, Chaheyla R St Aubin, Layla Al-Nakkash
BACKGROUND/AIMS: The goal of this study was to determine the influence of high-fat high-sugar diet (Western diet) on intestinal function and subsequently to determine if there were any beneficial effects of exercise, genistein (a naturally occurring phytoestrogen) or both, on the intestine. METHODS: We measured transepithelial short circuit current (Isc ), across freshly isolated segments of jejunum from male and female C57Bl/6J mice randomly assigned to one of the following groups for the 12-week study duration: high-fat high-sugar diet (HFS), HFS with genistein (Gen), HFS with exercise (Ex), or HFS with both genistein and exercise (Gen+Ex) and compared them to lean controls...
September 1, 2023: Cellular Physiology and Biochemistry