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Cellular Physiology and Biochemistry

Long Hai, Peidong Liu, Shengping Yu, Li Yi, Zhennan Tao, Chen Zhang, Iruni Roshanie Abeysekera, Tao Li, Luqing Tong, Haiwen Ma, Bo Liu, Yang Xie, Xingchen Zhou, Yu Lin, Meng Zhu, Kai Zhang, Bingcheng Ren, Haolang Ming, Yubao Huang, Xuejun Yang
BACKGROUND/AIMS: Jagged1 is the ligands of the Notch signaling and has been shown to promote glioma-initiating cells (GICs) in glioblastoma. The role of Jagged1 in GICs invasion and underlying molecular mechanisms remain unclear. METHODS: Survival data from R2 genomics analysis, the Cancer Genome Atlas (TCGA), the Chinese Glioma Genome Atlas (CGGA) and visualization platform database were used to evaluate the effects of Jagged1 on overall patient survival. we investigated Jagged1 induced the GICs cells' invasion by matrix degradation assays and Transwell cell invasion assays in vitro, then we further explored the underlying molecular mechanisms using Co-immunoprecipitation (co-IP) analysis...
December 21, 2018: Cellular Physiology and Biochemistry
Shousen Hu, Yongliang Yuan, Zhizhen Song, Dan Yan, Xiangzhen Kong
BACKGROUND/AIMS: Drug resistance remains a main obstacle to the treatment of non- small cell lung cancer (NSCLC). The aim of this study was to identify the expression profiles of microRNAs (miRNAs) in drug-resistant NSCLC cell lines. METHODS: The expression profiles of miRNAs in drug-resistant NSCLC cell lines were examined using miRNA sequencing, and the common dysregulated miRNAs in these cell lines were identified and analyzed by bioinformatics methods. RESULTS: A total of 29 upregulated miRNAs and 36 downregulated miRNAs were found in the drug-resistant NSCLC cell lines, of which 26 upregulated and 36 downregulated miRNAs were found to be involved in the Ras signaling pathway...
December 17, 2018: Cellular Physiology and Biochemistry
Yanan Yu, Kyosuke Shinohara, Huanming Xu, Zhenfeng Li, Tomoki Nishida, Hiroshi Hamada, Yuanqing Xu, Jingqi Zhou, Daisy Shao, Xiangchen Li, Duanduan Chen
BACKGROUND/AIMS: Nodal cilia that rotate in the ventral node play an important role in establishing left-right asymmetry during embryogenesis; however, inv mutant cilia present abnormal movement and induce laterality defects. The mechanism of their motility, which is regulated by dynein activation and microtubule arrangement, has not been fully understood. This study analyzed the dynein-triggered ciliary motion in the abnormal ultrastructure of the inv mutant, aiming to quantitatively evaluate the influence of microtubule mislocalization on the movement of the cilium...
December 14, 2018: Cellular Physiology and Biochemistry
Xiao Chen, Xiaoqun Li, Xiao Zhai, Xin Zhi, Liehu Cao, Longjuan Qin, Jiacan Su
BACKGROUND/AIMS: Bone homeostasis is associated with the balance between bone-resorbing osteoclasts and bone-forming osteoblasts. Unbalanced bone homeostasis as a result of reduced osteogenesis or excessive osteoclastogenesis can lead to disorders such as osteoporosis, Paget's disease, and rheumatoid arthritis. Shikimic acid is a cyclohexanecarboxylic acid, reported to exhibit pharmacological properties including anti-inflammatory and antioxidant activities. However, its effects on bone homeostasis remain unknown...
December 14, 2018: Cellular Physiology and Biochemistry
Lei Lang, Yixuan Hou, Yanlin Chen, Gang Tu, Jing Tao, Dan Yang, Lei Xi, Lixin Fu, Kexin Sun, Jiali Yin, Rui Chen, Meixi Peng, Shuiqing Liu, Manran Liu
BACKGROUND/AIMS: The ataxia-telangiectasia mutated (ATM) protein kinase is critical for the maintenance of genomic stability and acts as tumor suppressor. Although evidence shows that a DNA damage-independent ATM (oxidized ATM) may be involved in cancer progression, the underlying mechanism is still unclear. METHODS: Immunohistochemistry, immunofluorescence and western blotting were applied to detect the levels of oxidized ATM. Transwell assay was used to detect the cell migration and invasion abilities in different treatments...
December 14, 2018: Cellular Physiology and Biochemistry
Seok Choi, Hyun Goo Kang, Mei Jin Wu, Han Yi Jiao, Dong Hoon Shin, Chansik Hong, Jae Yeoul Jun
BACKGROUND/AIMS: Anoctamin1 (Ca2+-activated Cl- channel, ANO1) is a specific marker of the interstitial cells of Cajal (ICC) in the gastrointestinal tract, and are candidate proteins that can function as pacemaker channels. Recently, novel selective ANO1 inhibitors were discovered and used to study Ca2+-activated Cl- channels. Therefore, to investigate whether ANO1 channels function as pacemaker channels, selective ANO1 inhibitors were tested with respect to the pacemaker potentials in ICC...
December 14, 2018: Cellular Physiology and Biochemistry
Ying-Chun Liang, Yu-Peng Wu, Dong-Ning Chen, Shao-Hao Chen, Xiao-Dong Li, Xiong-Lin Sun, Yong Wei, Xu Ning, Xue-Yi Xue
BACKGROUND/AIMS: Accumulating evidence has shown that long non-coding RNAs (lncRNAs) in competing endogenous RNA (ceRNA) networks play crucial roles in tumor survival and patient prognosis; however, studies investigating ceRNA networks in pheochromocytoma (PCC) are lacking. In this study, we investigated the pathogenesis of PCC and whether lncRNAs acting through ceRNAs networks were associated with prognosis. METHODS: A total of 183 PCC samples and 3 control samples from The Cancer Genome Atlas database were analyzed...
December 14, 2018: Cellular Physiology and Biochemistry
Shuling Song, Jin Tan, Yuyang Miao, Zuoming Sun, Qiang Zhang
BACKGROUND/AIMS: Intermittent hypoxia (IH) causes apoptosis in pancreatic β-cells, but the potential mechanisms remain unclear. Endoplasmic reticulum (ER) stress, autophagy, and apoptosis are interlocked in an extensive crosstalk. Thus, this study aimed to investigate the contributions of ER stress and autophagy to IH-induced pancreatic β-cell apoptosis. METHODS: We established animal and cell models of IH, and then inhibited autophagy and ER stress by pharmacology and small interfering RNA (siRNA) in INS-1 cells and rats...
December 14, 2018: Cellular Physiology and Biochemistry
Chuanhai Zhang, Jing Jing Wang, Xiaoyun He, Chao Wang, Boyang Zhang, Jia Xu, Wentao Xu, Yunbo Luo, Kunlun Huang
BACKGROUND/AIMS: Brown and beige adipocytes are widely recognized as potential therapeutic targets to treat obesity and related metabolic disorders, and the recruitment of brown and beige adipocytes is an essential aspect that requires attention. Although many methods of activating brown adipocytes or generating beige adipocytes have been reported, the limited number and sources are the biggest challenges. The number of white adipocytes is much greater than the number of brown adipocytes, both in human adults and fetuses...
December 14, 2018: Cellular Physiology and Biochemistry
Yuxin Dai, Yongkun Wan, Mingke Qiu, Shuqing Wang, Chang Pan, Yang Wang, Jingmin Ou
BACKGROUND/AIMS: Dysregulation of long noncoding RNAs (lncRNAs) is associated with the proliferation and metastasis in a variety of cancers, of which lncRNA maternally expressed gene 3 (MEG3) has been indicated as a tumor suppressor in multiple malignancies. However, the underlying mechanisms by which MEG3 contributes to human hemangiomas (HAs) remain undetermined. METHODS: qRT-PCR analysis was performed to examine the expression levels of MEG3 and VEGF in proliferating or involuting phase HAs...
December 14, 2018: Cellular Physiology and Biochemistry
Jing Shen, Shoubo Cao, Xin Sun, Bo Pan, Jingyan Cao, Dehai Che, Shi Jin, Yingyue Cao, Ye Tian, Yan Yu
BACKGROUND/AIMS: Sonodynamic therapy (SDT) is expected to be a new method to solve the clinical problems caused by advanced metastasis in patients with lung cancer. The use of ultrasound has the advantage of being noninvasive, with deep-penetration properties. This study explored the anti-tumor effect of SDT with a new sonosensitizer, sinoporphyrin sodium (DVDMS), on the human small cell lung cancer H446 cell line in vitro and in vivo. METHODS: Absorption of DVDMS was detected by a fluorescence spectrophotometer, and DVDMS toxicity was determined using a Cell Counting Kit-8...
December 14, 2018: Cellular Physiology and Biochemistry
Zhiqin Li, Jia Wang, Qinglei Zeng, Chunling Hu, Jiajia Zhang, Hongyan Wang, Jingya Yan, Hua Li, Zujiang Yu
BACKGROUND/AIMS: HOTTIP is a critical modulator in human diseases including liver cancer, but its role and molecular biological mechanisms in liver fibrosis are still unclear. METHODS: The expression profile of HOTTIP during the progression of liver fibrosis was detected in human liver samples and in CCl4-treated mice using qRT-PCR. The expressing sh-HOTTIP adenoviral vector was used to reduce HOTTIP levels in vivo. Dual-Luciferase Reporter Assay was performed to validate the interaction between miR-148a and HOTTIP, TGFBR1, or TGFBR2...
December 13, 2018: Cellular Physiology and Biochemistry
Yung-Fong Tsai, Shun-Chin Yang, Wen-Yi Chang, Jih-Jung Chen, Chun-Yu Chen, Shih-Hsin Chang, Tsong-Long Hwang
BACKGROUND/AIMS: Formyl peptide receptors (FPRs) recognize different endogenous and exogenous molecular stimuli and mediate neutrophil activation. Dysregulation of excessive neutrophil activation and the resulting immune responses can induce acute lung injury (ALI) in the host. Accordingly, one promising approach to the treatment of neutrophil-dominated inflammatory diseases involves therapeutic FPR1 inhibition. METHODS: We extracted a potent FPR1 antagonist from Garcinia multiflora Champ...
December 12, 2018: Cellular Physiology and Biochemistry
Li-Hui Wang, Chun-Fu Wu, Nirmal Rajasekaran, Young Kee Shin
Cancer is a disease caused by the accumulation of genetic and epigenetic changes in two types of genes: tumor suppressor genes (TSGs) and proto-oncogenes. Extensive research has been conducted over the last few decades to elucidate the role of TSGs in cancer development. In cancer, loss of TSG function occurs via the deletion or inactivation of two alleles, according to Knudson's two-hit model hypothesis. It has become clear that mutations in TSGs are recessive at the level of an individual cell; therefore, a single mutation in a TSG is not sufficient to cause carcinogenesis...
December 12, 2018: Cellular Physiology and Biochemistry
Wenqing Xia, Yu-Chen Chen, Yong Luo, Dan-Feng Zhang, Huiyou Chen, Jianhua Ma, Xindao Yin
BACKGROUND/AIMS: Type 1 diabetes mellitus (T1DM) has been proven to be associated with an increased risk of cognitive dysfunction. In this study, we aimed to investigate whether disrupted spontaneous activity and functional connectivity (FC) exist in T1DM patients using resting-state functional magnetic resonance imaging (rs-fMRI) and to detect the relationships of these parameters with cognitive impairment. METHODS: T1DM patients (n=35) were compared with age-, sex-, and education level-matched healthy controls (n=50) through rs-fMRI...
December 12, 2018: Cellular Physiology and Biochemistry
Dan Li, Suyun Fan, Fei Yu, Xuchao Zhu, Yingchun Song, Meng Ye, Lihong Fan, Zhongwei Lv
BACKGROUND/AIMS: Forkhead box D1 (FOXD1) has a well-established role in early embryonic development and organogenesis and functions as an oncogene in several cancers. However, the clinical significance and biological roles of FOXD1 in non-small cell lung cancer (NSCLC) remain largely unknown. METHODS: A total of 264 primary NSCLC tissue samples were collected. The expression levels of FOXD1 in these samples were examined by immunohistochemical staining. The expression of FOXD1 was knocked down by lentiviral shRNA...
December 12, 2018: Cellular Physiology and Biochemistry
Manshu Yu, Jun Shi, Meixiao Sheng, Kun Gao, Lu Zhang, Li Liu, Yilin Zhu
BACKGROUND/AIMS: The epithelial-to-mesenchymal transition (EMT) of peritoneal mesothelial cells (PMCs) is a crucial event in the induction of peritoneal fibrosis (PF), in which canonical Wnt/β-catenin signaling participates. Smads signaling is reported to interact with β-catenin and synergistically regulates EMT. This study was aimed to reveal the effect of Astragalus on β-catenin in EMT of PMCs. METHODS: To obtain the role of β-catenin in EMT, gene transfer into HMrSV5 cell line and rats has been achieved...
December 12, 2018: Cellular Physiology and Biochemistry
Yan Wang, YuJie Cai, Haili Huang, Xiongjin Chen, Xiaoyi Chen, Xiaoting Chen, Hui Mai, Xiaohui Li, Jianghao Zhao, Jingqi Yang, Weihao Fan, Pei Tang, Yusen Chen, Keshen Li, Lili Cui
BACKGROUND/AIMS: Increasing evidence suggests the important role of sirtuin 2 (SIRT2) in the pathology of Parkinson's disease (PD). However, the association between potential functional polymorphisms in the SIRT2 gene and PD still needs to be identified. Exploring the molecular mechanism underlying this potential association could also provide novel insights into the pathogenesis of this disorder. METHODS: Bioinformatics analysis and screening were first performed to find potential microRNAs (miRNAs) that could target the SIRT2 gene, and molecular biology experiments were carried out to further identify the regulation between miRNA and SIRT2 and characterize the pivotal role of miRNA in PD models...
December 12, 2018: Cellular Physiology and Biochemistry
Felix Umrath, Carla Thomalla, Simone Pöschel, Katja Schenke-Layland, Siegmar Reinert, Dorothea Alexander
BACKGROUND/AIMS: Periosteal tissue is a valuable source of multipotent stem cells for bone tissue engineering. To characterize these cells in detail, we generated an immortalized human cranial periosteal cell line and observed an increased MSCA-1 and CD146 expression, as well as an earlier and stronger mineralization compared to the parental cells. Further, we detected a higher osteogenic potential of MSCA-1high compared to MSCA-1low cranial periosteal cell (CPC) fractions. In the present study, a possible synergism of MSCA-1 and CD146 for periosteal cell mineralization was investigated...
December 12, 2018: Cellular Physiology and Biochemistry
Qinghua Wu, Zhongxu Cao, Weiwei Xiao, Li Zhu, Qian Xie, Ling Li, Bao Zhang, Wei Zhao
BACKGROUND/AIMS: Glioblastoma multiforme (GBM) is a malignant and aggressive central nervous system (CNS) tumor with high mortality and low survival rate. Effective treatment of GMB is a challenge worldwide. Temozolomide (TMZ) is a drug used to treat GBM, while the survival period of GBM patients with positive treatment remains less than 15 months. Reactivating p53 and Inducing Tumor Apoptosis (RITA) is a novel potential anti-cancer small molecular drug. Thus, it is essential to discover novel targets or develop effective drugs combination strategy to treat GBM...
December 11, 2018: Cellular Physiology and Biochemistry
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