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Physiological Research

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https://read.qxmd.com/read/30904015/transient-increase-in-cellular-dehydrogenase-activity-after-cadmium-treatment-precedes-enhanced-production-of-reactive-oxygen-species-in-human-proximal-tubular-kidney-cells
#1
J Handl, J Čapek, P Majtnerová, F Petira, M Hauschke, E Roušarová, T Roušar
Cadmium is a heavy metal causing toxicity especially in kidney cells. The toxicity is linked also with enhanced oxidative stress leading to cell death. On the other hand, our recent experiments have shown that an increase of total intracellular dehydrogenases activity can also occur in kidney cells before declining until cell death. The aim of the present study, therefore, was to evaluate this transient enhancement in cell viability after cadmium treatment. The human kidney HK-2 cell line was treated with CdCl(2) at concentrations 0-200 mikroM for 2-24 h and intracellular dehydrogenase activity was tested...
March 22, 2019: Physiological Research
https://read.qxmd.com/read/30904014/metabolic-and-renal-effects-of-dietary-advanced-glycation-end-products-in-pregnant-rats-a-pilot-study
#2
K Janšáková, E Lengyelová, N Pribulová, V Somoza, P Celec, K Šebeková, Ľ Tóthová
Thermally processed food contains advanced glycation end products (AGEs) including Nepsilon-(carboxymethyl)lysine (CML). Higher AGEs or circulating CML were shown to be associated with pregnancy complications such as preeclampsia and gestational diabetes. It is unclear whether this association is causal. The aim of our study was to analyze the effects of dietary CML and CML-containing thermally processed food on the metabolism in pregnant rats. Animals were fed with standard, with AGE-rich diet from gestation day 1...
March 22, 2019: Physiological Research
https://read.qxmd.com/read/30904013/electroacupuncture-enhances-antioxidative-signal-pathway-and-attenuates-neuropathic-pain-induced-by-chemotherapeutic-paclitaxel
#3
X Zhao, L Liu, Y Wang, G Wang, Y Zhao, Y Zhang
One of the significant limiting complications of paclitaxel is painful peripheral neuropathy during its therapy for several types of cancers. Our recent study showed that impairment of Nrf2-antioxidant response element (Nrf2-ARE) and upregulation of oxidative signals in the dorsal root ganglion (DRG) of rats with treatment of paclitaxel result in neuropathic pain. The purpose of this study was to examine the beneficial role played by electroacupuncture (EA) in modifying neuropathic pain evoked by paclitaxel via Nrf2-ARE and oxidative mechanisms...
March 22, 2019: Physiological Research
https://read.qxmd.com/read/30904012/relationship-between-steroid-hormones-and-metabolic-profile-in-women-with-polycystic-ovary-syndrome
#4
I Lazúrová, Z Lazúrová, J Figurová, S Ujházi, I Dravecká, J Mašlanková, M Mareková
Polycystic ovary syndrome (PCOS) is commonly associated with a higher cardiometabolic risk. The relationship between steroid hormones and cardiometabolic profile in PCOS has been evaluated, but no single hormonal predictor of this association has been identified. To determine the relationship between steroid hormones and cardiometabolic risk factors in PCOS women. Study included 64 women diagnosed with PCOS. Fasting blood samples were analyzed for biochemical, metabolic parameters and sex steroid hormones. PCOS women with BMI 27 had significantly higher serum free testosterone (FT), free androgen index (FAI), estrone (E1) (p=0...
March 22, 2019: Physiological Research
https://read.qxmd.com/read/30904011/assessment-of-platelet-respiration-as-emerging-biomarker-of-disease
#5
A T Petrus, D L Lighezan, M D Dănilă, O M Duicu, A Sturza, D M Muntean, I Ionită
Mitochondrial dysfunction is currently acknowledged as a central pathomechanism of most common diseases of the 21st century. Recently, the assessment of the bioenergetic profile of human peripheral blood cells has emerged as a novel research field with potential applications in the development of disease biomarkers. In particular, platelets have been successfully used for the ex vivo analysis of mitochondrial respiratory function in several acute and chronic pathologies. An increasing number of studies support the idea that evaluation of the bioenergetic function in circulating platelets may represent the peripheral signature of mitochondrial dysfunction in metabolically active tissues (brain, heart, liver, skeletal muscle)...
March 22, 2019: Physiological Research
https://read.qxmd.com/read/30904010/association-of-pigment-epithelium-derived-factor-with-von-willebrand-factor-and-plasminogen-activator-inhibitor-1-in-patients-with-type-2-diabetes
#6
D Karasek, J Spurna, V Kubickova, O Krystynik, L Cibickova, J Schovanek, D Goldmannova
To compare circulating pigment epithelium derived factor (PEDF) levels in type 2 diabetes patients (T2D) with and without metabolic syndrome (MetS+/-) to healthy controls and assess PEDF association with plasminogen activator inhibitor-1 (PAI-1) and von Willebrand factor (vWF) as markers of endothelial dysfunction. Fifty T2D individuals and forty healthy controls were included. PEDF, PAI-1, vWF, anthropological parameters, lipids, and markers of insulin resistance were investigated in all subjects. Compared to controls only MetS+ diabetics had higher PEDF levels [14...
March 22, 2019: Physiological Research
https://read.qxmd.com/read/30904009/correlation-of-tumor-necrosis-factor-alpha-interleukin-6-and-interleukin-10-with-blood-pressure-risk-of-preeclampsia-and-low-birth-weight-in-gestational-diabetes
#7
P Žák, M Souček
Several pathophysiological mechanisms have been proposed in the development of pregnancy complications, including endothelial dysfunction, an inflammatory pathway and oxidative stress. The aim of the present study was to evaluate the correlation between proinflammatory cytokines TNF-alpha, IL-6 and dual cytokine IL-10 in the mother's peripheral blood and blood pressure, risk of preeclampsia and low birth weight in gestational diabetes (GDM). We observed 40 women with GDM divided into a gestational hypertension group (n=20) and comparison group (n=20) with normal blood pressure...
March 22, 2019: Physiological Research
https://read.qxmd.com/read/30904008/hydrogen-sulfide-plays-an-important-protective-role-by-influencing-autophagy-in-diseases
#8
J Wang, D Wu, H Wang
Autophagy can regulate cell growth, proliferation, and stability of cell environment. Its dysfunction can be involved in a variety of diseases. Hydrogen sulfide (H(2)S) is an important signaling molecule that regulates many physiological and pathological processes. Recent studies indicate that H(2)S plays an important protective role in many diseases through influencing autophagy, but its mechanism is not fully understood. This article reviewed the progress about the effect of H(2)S on autophagy in diseases in recent years in order to provide theoretical basis for the further research on the interaction of H(2)S and autophagy and the mechanisms involved...
March 22, 2019: Physiological Research
https://read.qxmd.com/read/30904007/various-akip1-expression-levels-affect-its-subcellular-localization-but-have-no-effect-on-nf-kappab-activation
#9
A Keprová, L Kořínková, I Křížová, R Hadravová, F Kaufman, I Pichová, T Ruml, M Rumlová
A-kinase interacting protein 1 (AKIP1) has been shown to interact with a broad range of proteinsinvolved in various cellular processes, including apoptosis, tumorigenesis, and oxidative stress suggesting it might have multiple cellular functions. In this study, we used an epitope-tagged AKIP1 and by combination of immunochemical approaches, microscopic methods and reporter assays we studied its properties. Here, we show that various levels of AKIP1 overexpression in HEK-293 cells affected not only its subcellular localization but also resulted in aggregation...
March 22, 2019: Physiological Research
https://read.qxmd.com/read/30904006/the-effect-of-repeated-whole-body-cryostymulation-on-the-hsp-70-and-lipid-metabolisms-in-healthy-subjects
#10
A Lubkowska, I Bryczkowska, Z Szygula, C Giemza, A Skrzek, I Rotter, G Lombardi, G Banfi
The aim of this study was to evaluate the effects of exposure to 30 daily whole body cryostimulation (WBC) on lipid metabolic parameters and serum HSP-70 concentration. The study involved 45 volunteers, homogeneous in terms of diet and daily physical activity. Blood samples were collected before and after the 10th, the 20th, and the 30th session and one month after the intervention. Total cholesterol, HDL, TG concentrations and Apolipoprotein A-I, ApoB and HSP-70 protein levels were determined in serum. Additionally, the LI (Lipid Index) and the LDL level were calculated...
March 22, 2019: Physiological Research
https://read.qxmd.com/read/30904005/cardioprotective-properties-of-opioid-receptor-agonists-in-rats-with-stress-induced-cardiac-injury
#11
E S Prokudina, L N Maslov, N V Naryzhnaya, S Y Tsibulnikov, Y B Lishmanov, J E Madias, P R Oeltgen
The objectives of this study were to investigate the role of endogenous opioids in the mediation of stress-induced cardiomyopathy (SIC), and to evaluate which opioid receptors regulate heart resistance to immobilization stress. Wistar rats were subjected to 24 h immobilization stress. Stress-induced heart injury was assessed by 99mTc-pyrophosphate accumulation in the heart. The opioid receptor (OR) antagonists (naltrexone, NxMB - naltrexone methyl bromide, MR 2266, ICI 174.864) and agonists (DALDA, DAMGO, DSLET, U-50,488) were administered intraperitoneally prior to immobilization and 12 h after the start of stress...
March 22, 2019: Physiological Research
https://read.qxmd.com/read/30904004/galanin-receptor-1-plays-an-antinociceptive-effect-via-inhibiting-pka-activation-in-the-nucleus-accumbens-of-rats-with-neuropathic-pain
#12
Y Zhang, Y Gao, C-Y Li, W Dong, Y Dong, M-N Li, Y-N Liu, S-L Xu
Galanin and galanin receptors (GalRs) have been reported to be involved in the transmission and modulation of nociceptive information in the central nervous system (CNS). However, the underlying mechanism of the antinociception of GalRs in neuropathic pain remains unclear. This study investigated the antinociception induced by galanin receptor 1 (GalR1) via protein kinase A (PKA) signalling pathway in the nucleus accumbens (NAc) of rats with neuropathic pain. A mononeuropathy model was replicated by ligation of the left sciatic nerve, following which the expression of phospho-PKA (p-PKA) in the NAc were markedly up-regulated at 14th and 28th day after ligation of sciatic nerve, and p-PKA expression was down-regulated by intra-NAc injection of GalR1 agonist M617, but the GalR1 antagonist M35 did not have an effect...
March 22, 2019: Physiological Research
https://read.qxmd.com/read/30904003/impacts-of-different-anesthetic-agents-on-left-ventricular-systolic-function-in-mice-assessed-by-echocardiography
#13
C Li, J Dai, F Wu, H Zhang
The present experiments were performed to study the effects and time trends of different anesthetic agents on the left ventricular (LV) systolic function and heart rate by high-resolution echocardiography in mice. Ten male C57BL/6J mice were submitted to echocardiography imaging separated by 72-hour intervals under the following conditions: 1) conscious mice, 2) mice anesthetized with isoflurane (ISO, inhaled), 3) mice anesthetized with tribromoethanol (TBE, intraperitoneal), 4) mice anesthetized with chloral hydrate (CH, intraperitoneal), and 5) mice anesthetized with pentobarbital sodium (PS, intraperitoneal)...
March 22, 2019: Physiological Research
https://read.qxmd.com/read/30904002/gene-expression-in-patients-with-abdominal-aortic-aneurysm-more-than-immunological-mechanisms-involved
#14
M Prucha, P Sedivy, P Stadler, P Zdrahal, V Matoska, H Strnad
Abdominal aortic aneurysm (AAA) is a serious condition of unclear pathogenesis and progression. Two samples were collected from 48 patients during AAA surgery. One sample was collected from the aneurysm, the other from the aneurysm proximal neck where the tissue did not exhibit any aneurysmal changes. Subsequently, gene expression profiles using microarrays (Illumina) were compared in RNA extracted from the samples. Overall, 2,185 genes were found to be upregulated and 2,100 downregulated; from which 158 genes had a different expression with FDR<0...
March 22, 2019: Physiological Research
https://read.qxmd.com/read/30848156/coenzyme-q10-ameliorates-cadmium-induced-reproductive-toxicity-in-male-rats
#15
R Saha, S Roychoudhury, K Kar, A C Varghese, P Nandi, G D Sharma, G Formicki, P Slama, A Kolesarova
This study aimed at investigating the protective role of CoQ10 against cadmium (Cd)-induced reproductive toxicity in male rats. Adult male Wistar rats were exposed to an acute dose of Cd (25 mg/kg bwt; Cd group), Cd+CoQ10 (25 mg/kg bwt Cd+10 mg CoQ10; Cd-Q10 group) and distilled water (control) in vivo for 15 consecutive days and semen quality was assessed. A significant reduction was noted in sperm concentration, progressive motility, morphology and DNA integrity in both Cd- and Cd-Q10 groups in comparison to control indicating Cd-induced testicular lipid per oxidation (LPO) and decline in indigenous antioxidant defense system as measured by total antioxidant capacity (TAC) (p<0...
March 6, 2019: Physiological Research
https://read.qxmd.com/read/30848155/renal-sympathetic-denervation-in-resistant-arterial-hypertension-long-term-and-updated-results
#16
J H Peregrin, J Novotný, T Rohál, J Šochman
The present paper is an extension to our earlier publication (Šochman et al. 2016) documenting a beneficial effect of renal sympathetic denervation on pharmacologically uncontrollable hypertension in a group of seven patients followed up for 1-2 years post-procedure. The same patients remained on ambulatory follow-up for another 5-6 years, with the beneficial effect persisting throughout the follow-up period while on the same medication.
March 6, 2019: Physiological Research
https://read.qxmd.com/read/30628838/analysis-of-ca-2-signaling-mechanisms-our-experience-on-the-intercellular-communication-in-muscle-remodeling
#17
S Filip, J Mokrý, O Forostyak, G Dayanithi
The aim of this study was to evaluate cell diversity by considering how Ca(2+) signaling has been adapted in skeletal muscle cell function. We characterized single C2C12 myoblasts through intracellular Ca(2+) signaling kinetics after exposure to specific drugs and calcium blockers using fast fluorescence microspectrofluorimetry followed by ATP effect analysis, which confirmed the expression of functional purinergic adenosine and P2 receptors. Further, we found that glutamate sensitivity of C2C12 cells was mediated by ionotropic glutamate receptors; on the other hand, most cells were responsive to cyclopiazonic acid, which inhibits the sarco-endoplasmic reticulum Ca(2+)-ATPase pump...
January 10, 2019: Physiological Research
https://read.qxmd.com/read/30628837/the-extracellular-matrix-and-ca-2-signaling-mechanisms
#18
S Filip, J Mokrý, O Forostyak, G Dayanithi
The extracellular matrix (ECM) consists of proteins, glycosaminoglycans and glycoproteins, that support the dynamic interactions between cells, including intercellular communication, cell attachment, cell differentiation, cell growth and migration. As such, the ECM represents an essential and very sensitive system within the tissue microenvironment that is involved in processes such as tissue regeneration and carcinogenesis. The aim of the present review is to evaluate its diversity through Ca(2+) signaling and its role in muscle cell function...
January 10, 2019: Physiological Research
https://read.qxmd.com/read/30628836/loss-of-body-weight-is-accompanying-cellular-brain-edema-induced-by-water-intoxication-in-the-rat
#19
P Kozler, D Marešová, J Pokorný
Induction of cellular cerebral edema (CE) was achieved by a standard method of water intoxication which consisted of fractionated intraperitoneal administration of distilled water (DW) together with the injection of desmopressin (DP). Using metabolic cage, fluid and food balance was studied in two groups of eight animals: group C - control; group CE - cellular edema induced by water intoxication. For each rat the intake (food pellets and water) and excretion (solid excrements and urine) were recorded for 48 h together with the initial and final body weight...
January 10, 2019: Physiological Research
https://read.qxmd.com/read/30628835/gastrointestinal-non-motor-dysfunction-in-parkinson-s-disease-model-rats-with-6-hydroxydopamine
#20
Jing-Ting Yan, Xiao-Yan Feng
Parkinson's disease (PD) is a neurodegenerative disease with a progressive loss of mesencephalic dopaminergic neurons of the substantia nigra (SN). To further evaluate its pathophysiology, accurate animal models are needed. The current study aims to verify the impact of a 6-hydroxydopamine (6-OHDA) bilateral microinjection into the SN on gastrointestinal symptoms in rats and confirm that the 6-OHDA rat model is an appropriate tool to investigate the mechanisms of Parkinsonian GI disorders. Immunohistochemistry, digital X-ray imaging, short-circuit current, FITC-dextran permeability and ultra-performance liquid chromatography tandem mass spectrometry were used in this study...
January 10, 2019: Physiological Research
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