Ganesh P Subedi, Elijah T Roberts, Alexander R Davis, Paul G Kremer, I Jonathan Amster, Adam W Barb
A large proportion of human proteins contain post-translational modifications that cannot be synthesized by prokaryotes. Thus, mammalian expression systems are often employed to characterize structure/function relationships using NMR spectroscopy. Here we define the selective isotope labeling of secreted, post-translationally modified proteins using human embryonic kidney (HEK)293 cells. We determined that alpha-[15 N]- atoms from 10 amino acids experience minimal metabolic scrambling (C, F, H, K, M, N, R, T, W, Y)...
February 26, 2024: Journal of Biomolecular NMR