journal
MENU ▼
Read by QxMD icon Read
search

Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission

journal
https://read.qxmd.com/read/30760053/effects-of-hcg-on-reduced-numbers-of-hcg-receptors-in-the-prefrontal-cortex-and-cerebellum-of-rat-models-of-alzheimer-s-disease
#1
M Jahanshahi, M Saeidi, E Nikmahzar, F Babakordi, G Bahlakeh
Age-associated changes in the levels of luteinizing hormone and human chorionic gonadotropin (hCG) are potential risk factors for Alzheimer's disease (AD); hCG concentration is related to the incidence of AD. The highest density of hCG receptors is in zones of the brain that are vulnerable to AD and streptozotocin (STZ) can decrease the density of this receptor. We investigated the effects of different doses of hCG on hCG receptor density in the prefrontal cortex and cerebellum in a rat model of STZ-induced AD...
February 14, 2019: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://read.qxmd.com/read/30729828/spectroscopic-and-molecular-docking-investigation-of-polynucleotides-and-dna-binding-affinity-to-nile-blue-dye
#2
H Dezhampanah, R Firouzi, S Darvishi
We used UV-vis absorption spectroscopy, fluorescence spectrophotometry and molecular docking calculations to investigate intermolecular interaction between the cationic dye, Nile blue (NB), and synthetic polynucleotides, poly(A-T), poly(G-C) and calf thymus DNA (Ct-DNA) at physiological pH. Strong hypsochromic absorbance and fluorescence quenching were observed that showed strong binding of NB to these polynucleotides and DNA. The binding affinity values derived from maximum absorption of the spectra of NB bound to various polynucleotides and Ct-DNA concentrations suggests that NB exhibits greater binding affinity to poly(G-C) than to poly(A-T)...
February 7, 2019: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://read.qxmd.com/read/30729816/immunohistochemical-evaluation-of-natural-cases-of-encephalitic-listeriosis-in-sheep
#3
M Haligur, A Aydogan, O Ozmen, V Ipek
Listeriosis is an important public health problem in the world. It can cause abortion, encephalitis, septicemia, conjunctivitis and mastitis in ruminants. The development of central nervous system lesions is not fully understood in encephalitic listeriosis. We performed a retrospective analysis of 15 sheep with encephalitic listeriosis. Hyperemia and opacification of the meninges were common necropsy findings. Lesions generally were localized in the caudal part of the brain including the pons, medulla oblongata, thalamus and cerebellum...
February 7, 2019: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://read.qxmd.com/read/30729815/expression-and-localization-of-npr2-in-mouse-oocytes-and-pre-implantation-embryos
#4
H Xi, L Lei, W Fu, L Li, X Cao, L Yang
We investigated the expression and localization of natriuretic peptide receptor 2 (Npr2) in mouse oocytes and pre-implantation embryos. Immunocytochemistry and quantitative real-time polymerase chain reaction (qRT-PCR) were performed on mouse oocytes and embryos at different developmental stages. Npr2 was expressed in all oocyte and pre-implantation embryo stages analyzed. Germinal vesicle oocytes exhibited greater Npr2 expression than metaphase II oocytes. In pre-implantation embryos, Npr2 expression was significantly greater from the 2-cell stage to the blastocyst stage than for the zygote...
February 7, 2019: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://read.qxmd.com/read/30712392/hepatoprotective-potential-of-ferula-communis-extract-for-carbon-tetrachloride-induced-hepatotoxicity-and-oxidative-damage-in-rats
#5
G Y Deniz, E Laloglu, K Koc, F Geyikoglu
We investigated the hepatoprotective potential of Ferula communis extract for CCI4 induced liver damage. We used six groups of rats: group 1, untreated control; group 2, CCl4 treated (hepatotoxic); group 3, treated with 150 mg/kg F. communis; group 4, treated with 300 mg/kg F. communis; group 5, treated with CCl4  + 150 mg/kg F. communis; and group 6, treated with CCl4  + 300 mg/kg F. communis. Liver damage was produced by injection of 1 ml/kg CCI4 twice/week. Extracts of F. communis, 150 and 300 mg/kg/day, were administered for 8 weeks...
February 4, 2019: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://read.qxmd.com/read/30712389/potential-involvement-of-s1pr1-stat3-signaling-pathway-in-cardiac-valve-damage-due-to-rheumatic-heart-disease
#6
X-D Wu, Z-Y Zeng, D-P Gong, J-L Wen, F Huang
Rheumatic heart disease (RHD) is a public health burden in developing countries. Th17 cell-associated cytokines might play a role in the pathogenesis and development of RHD, but the specific molecular mechanism is not completely understood. We investigated the potential role of sphingosine-1-phosphate receptor 1 (S1PR1)/signal transducer and activator of transcription 3 (STAT3) signaling pathway in cardiac valve damage in a rat model of RHD. We used 20 Lewis rats divided randomly into control and RHD groups...
February 4, 2019: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://read.qxmd.com/read/30712387/comparison-of-myotoxic-effects-of-levobupivacaine-bupivacaine-and-ropivacaine-apoptotic-activity-and-acute-effect-on-pro-inflammatory-cytokines
#7
O Oz Gergin, A Bayram, I S Gergin, R Aksu, A Yay, E Balcıoglu, S Polat, G Coşkun, Z Soyer Sarıca, K Yıldız
We investigated the myotoxic effects of bupivacaine, ropivacaine and levobupivacaine on rat skeletal muscle and compared its apoptotic activity and acute effects on pro-nflammatory cytokines. We divided 40 Wistar albino rats into four equal groups. Rats were injected intramuscularly with 0.5% bupivacaine (group B), 0.5% ropivacaine (group R), 0.5% levobupivacaine (group L) or 0.9% normal saline (group SF). Animals were sacrificed on the second day after the injection. TNF-α, IL-1 and IL-6 levels were examined in muscle tissue using immunohistochemistry and immunofluorescence...
February 4, 2019: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://read.qxmd.com/read/30669870/protective-effects-of-luteolin-on-rat-testis-following-exposure-to-900-mhz-electromagnetic-field
#8
A Yahyazadeh, B Z Altunkaynak
Increasing cell phone use calls for clarification of the consequences of long term exposure to electromagnetic fields (EMF). We investigated the effects of EMF on the testes of 12-week-old rats as well as possible protective effects of luteolin on testis tissue. Twenty-four Wistar albino rats were randomly divided into four groups: control, EMF, luteolin, and EMF + luteolin. The number of Leydig cells, primary spermatocytes and spermatids were reduced in the EMF group compared to the control group. In the EMF + luteolin group, the number of Leydig cells, primary spermatocytes and spermatids was significantly greater than the EMF group...
January 23, 2019: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://read.qxmd.com/read/30632398/effects-of-alpha-lipoic-acid-on-high-fructose-induced-hepatic-pathology
#9
S Topsakal, O Ozmen, M Ozgocmen
Little is known about the pathogenesis of high fructose corn syrup (HFCS) induced hepatic toxicity. We investigated hepatic lesions induced by chronic HFCS consumption and the protective effects of alpha-lipoic acid (ALA) on liver pathology. We used 24 rats allocated randomly into three groups of eight. The HFCS group was given in drinking water for 10 weeks. The ALA + HFCS group was given the same dose of HFCS and ALA also was administered during the last 6 weeks of the experiment. The control group was untreated...
January 11, 2019: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://read.qxmd.com/read/30605014/hypoxia-inducible-factor-1-alpha-and-vascular-endothelial-growth-factor-in-cartilage-tumors
#10
E Kouvaras, Z Christoni, I Siasios, K Malizos, G K Koukoulis, M Ioannou
Neoangiogenesis has been demonstrated in chondrosarcoma. Anti-angiogenic therapies are being tested in clinical trials for chondrosarcomas. Studies of the underlying mechanisms have been performed almost exclusively in cell lines. We immunostained 20 samples of chondrosarcoma and 20 samples of enchondromas with antibodies against hypoxia-inducible factor 1-alpha (HIF-1-alpha) and vascular endothelial growth factor (VEGF). The immunohistochemical staining of HIF-1-alpha and VEGF were highly correlated. Enchondromas were HIF-1-alpha and VEGF negative, whereas all chondrosarcoma exhibited HIF-1-alpha and VEGF immunostaining...
January 3, 2019: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://read.qxmd.com/read/30556450/a-bone-preservation-protocol-that-enables-evaluation-of-osseointegration-of-implants-with-micro-and-nanotextured-surfaces
#11
M F Kunrath, R Hübler
Development of surface treatments has enabled secure attachment of dental implants in less than 1 month. Consequently, it is necessary to characterize accurately the osseointegration of the implant surface in the region of the bone-implant contact (BIC). We developed a method for sample preparation that preserves both bone and BIC to permit analysis of the contact interface. We prepared eight nanotextured implants and implanted them in rabbit tibias. After healing for 30 days, outcomes were analyzed using both our bone preservation protocol and routine decalcification followed by preparation of histological sections stained by hematoxylin and eosin (H & E)...
December 17, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://read.qxmd.com/read/30556436/investigation-of-new-indole-derivatives-of-bovine-serum-albumin-using-spectroscopic-and-molecular-docking-techniques
#12
H Dezhampanah, M Esmaili, A Akbarnia Dafrazi, P Mehdizadeh
We investigated the interaction of two derivatives of bis (indolyl) methane with bovine serum albumin (BSA) using spectroscopic and molecular docking calculations. Fluorescence quenching measurements revealed that the quenching mechanism was static. Fӧrster energy transfer measurements, synchronous fluorescence spectroscopy and docking studies demonstrated that both bis(indolyl)methanes bound to the Trp residues of BSA. The docking study confirmed that both bis(indolyl)methanes form hydrogen bonds and van der Waals interactions with BSA...
December 17, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://read.qxmd.com/read/30516072/quirks-of-dye-nomenclature-11-safranine-and-its-relatives
#13
C J Cooksey
Safranine was one of the earliest coal tar dyes following mauveine. By the end of the 19th century, many alkylated derivatives of safranine had been made. The history, identity, names, manufacture, analysis, toxicity, textile dyeing, and biological staining applications, plus some nonstaining uses of safranine, phenosafranine, methylene violet, amethyst violet, azocarmine, and Magdala red are described here.
December 5, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://read.qxmd.com/read/30516069/rat-lung-decellularization-using-chemical-detergents-for-lung-tissue-engineering
#14
H Tebyanian, A Karami, E Motavallian, A Samadikuchaksaraei, B Arjmand, M R Nourani
Although pulmonary diseases account for a large number of deaths in the world, most have no treatment other than transplantation. New therapeutic methods for lung treatment include lung tissue engineering and regenerative medicine. Lung decellularization has been used to produce an appropriate scaffold for recellularization and implantation. We investigated 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) with sodium dodecyl sulfate (SDS) and Triton X-100 detergents for effecting rat lung decellularization...
December 5, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://read.qxmd.com/read/30512973/-formula-see-text-a-tribute-to-hans-o-lyon-1932-2018
#15
G S Nettleton, Richard Dapson, Richard W Horobin
No abstract text is available yet for this article.
December 4, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://read.qxmd.com/read/30512970/biochemical-and-morphological-evaluation-of-the-effects-of-propolis-on-cisplatin-induced-kidney-damage-in-rats
#16
E Yuluğ, S Türedi, Ö Yıldırım, E Yenilmez, Y Aliyazıcıoğlu, S Demir, S Özer-Yaman, A Menteşe
Cisplatin (CP) is a chemotherapeutic agent used to treat various types of cancer; nephrotoxicity is the most common adverse effect of the drug. We investigated the protective effects of propolis against CP induced kidney injury. Thirty-six male rats were divided into six equal groups: untreated control group, 50 mg/kg/day propolis group, 100 mg/kg/day propolis group, single-dose 7 mg/kg CP group, 7 mg/kg CP + 50 mg/kg/day propolis and 7 mg/kg CP + 100 mg/kg propolis. Rats were sacrificed after 14 days and kidneys were removed for histopathological and biochemical analyses...
December 4, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://read.qxmd.com/read/30460873/keratinocytes-derived-from-embryonic-stem-cells-induce-wound-healing-in-mice
#17
E T Uluer, H S Vatansever, H Aydede, M K Ozbilgin
The skin plays an important role in defending the body against the environment. Treatments for burns and skin injuries that use autologous or allogenic skin grafts derived from adult or embryonic stem cells are promising. Embryonic stem cells are candidates for regenerative and reparative medicine. We investigated the utility of keratinocyte-like cells, which are differentiated from mouse embryonic stem cells, for wound healing using a mouse surgical wound model. Mice were allocated to the following groups: experimental, in which dressing and differentiated cells were applied after the surgical wound was created; control, in which only the surgical wound was created; sham, in which only the dressing was applied after the surgical wound was created; and untreated animal controls with healthy skin...
November 21, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://read.qxmd.com/read/30449191/effects-of-nicotine-on-rat-adrenal-gland-crosstalk-between-oxidative-and-inflammatory-markers-and-amelioration-by-melatonin
#18
S Abdel Fattah, A A E-E Rizk, A G Motawie, T I Abd El-Galil, M El Sebaie
Although the risks of smoking are well known, the effects of exposure to nicotine on endocrine functions remain unclear. We investigated the deleterious effects of nicotine on the adrenal gland and the mechanisms of these changes in rats. The role of melatonin in ameliorating pathological changes also was investigated. We used 24 rats divided into four groups of six: group 1, control; group 2, nicotine treated; group 3, nicotine and melatonin treated; group 4, melatonin treated. We used histology; immunohistochemistry of inducible nitric oxide synthase (iNOS), vascular endothelial growth factor (VEGF) and tyrosine hydroxylase (TH); measured oxidative and antioxidative markers, malondialdehyde (MDA) and glutathione (GSH); and performed real-time PCR for NF-κB 65, IL1-B and IL6...
November 19, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://read.qxmd.com/read/30449186/effects-of-subacute-3-monochloropropane-1-2-diol-treatment-on-the-kidney-of-male-albino-rats
#19
Y I Mahmoud, F S Abo-Zied, S T Salem
3-Monochloropropane-1,2-diol (3-MCPD) is a well-known food contaminant. Although the kidney is thought to be a target organ for 3-MCPD toxicity, nephrotoxic structural changes are relatively unstudied. We investigated the renal alterations caused by 3-MCPD in male albino rats. 3-MCPD was administered orally, at a dose of 60 mg/kg for 7 days. 3-MCPD caused significant elevation of serum creatinine and urea levels together with hydropic degeneration, necrosis and shedding of the cells of the proximal convoluted tubules, urinary casts in the distal convoluted tubules and interstitial inflammatory cell infiltration...
November 19, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://read.qxmd.com/read/30409033/wound-healing-effects-of-methanol-extract-of-laurocerasus-officinalis-roem
#20
S Ayla, M E Okur, M Y Günal, E M Özdemir, D Çiçek Polat, A Yoltaş, Ö Biçeroğlu, S Karahüseyinoğlu
Laurocerasus officinalis Roem. (syn: Prunus laurocerasus L.) is a member of Rosaceae family. We investigated the antimicrobial and antioxidant activity of L. officinalis Roem in wound healing both in vivo and in vitro using an excisional wound model model in mice. We used four groups of eight mice as follows: untreated (control), empty gel, extract + gel (L. officinalis + gel), and Madecassol® groups. All treatments were applied topically once daily. The scar area, percentage wound closure and epithelization time were measured...
November 9, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
journal
journal
30733
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"