Leila Allahgholi, Maik G N Derks, Justyna M Dobruchowska, Andrius Jasilionis, Antoine Moenaert, Léonie Jouy, Kazi Zubaida Gulshan Ara, Javier A Linares-Pastén, Ólafur H Friðjónsson, Guðmundur Óli Hreggviðsson, Eva Nordberg Karlsson
The marine environment, contains plentiful renewable resources, e.g. macroalgae with unique polysaccharides, motivating search for enzymes from marine microorganisms to explore conversion possibilities of the polysaccharides. In this study, the first GH17 glucanosyltransglycosylase, MlGH17B, from a marine bacterium (Muricauda lutaonensis), was characterized. The enzyme was moderately thermostable with Tm at 64.4 °C and 73.2 °C, but an activity optimum at 20 °C, indicating temperature sensitive active site interactions...
January 25, 2024: Glycobiology