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Acta Cirúrgica Brasileira

Eren Altun, Ayla Yildiz, Celalettin Cevik, Gulay Turan
PURPOSE: To evaluate serum levels of high-sensitivity C-reactive protein (hs-CRP) in chronic gastritis patients to predict Helicobacter pylori (HP) infection, inflammatory activity, and precancerous lesions. METHODS: A total of 811 patients with upper gastrointestinal symptoms and histopathological diagnosis of chronic gastritis were enrolled in the study. On endoscopy, five gastric biopsies were taken according to Modified Sydney protocol, which were stained with hematoxylin & eosin and Giemsa...
March 21, 2019: Acta Cirúrgica Brasileira
Leonardo Waldstein de Moura Vidal, Paula Gebe Abreu Cabral, Marcelo Borges Dos Santos Junior, Fernanda Antunes, Matheus Roberto da Mota, Tomas Ottoni Barroso da Silva, Guilherme Alexandre Soares Monteiro, Jussara Peters Scheffer, Mariana da Silva Ribeiro, André Lacerda de Abreu Oliveira
PURPOSE: To evaluate, in rats, the open field videothermometry in real time while performing left pneumonectomy for early diagnosis of cardiopulmonary changes. METHODS: Twelve non-specific pathogen-free Wistar rats were randomly allocated into two groups; pneumectomy group (GP) and sham surgery group (GS). Mean arterial pressure, videothermometry in real time, of the right lung, and histopathological analysis of the remaining lung were evaluated in all animals. RESULTS: Videothermometry in real time allowed identification of temperature variance of right lung after pneumectomy, indicating a significant decrease in temperature during evaluation...
March 21, 2019: Acta Cirúrgica Brasileira
Oguzhan Yeniay, Zeki Tuncel Tekgul, Onur Okur, Noyan Koroglu
PURPOSE: To measure the preoperative fasting durations with respect to time of the day and its effect on vital parameters and electrocardiogram in elderly patients undergoing surgery under spinal anesthesia. METHODS: This study investigated 211 patients older than 60 years undergoing elective surgery under spinal anesthesia. Patients scheduled for surgery in morning hours (AM) and afternoon hours (PM) were compared. Patients fasting hours and repeated measurements of mean arterial pressure (MAP), heart rate (HR), peripheral oxygen saturation (Sp02) and the type and number of ischemic electrocardiogram (ECG) signs were recorded and compared [preoperative, zeroth, 2nd,5th,15th,30th minutes following spinal anesthesia(SA)]...
March 18, 2019: Acta Cirúrgica Brasileira
Márcio Alencar Barreira, Delano Gurgel Siveira, Hermano Alexandre Lima Rocha, Luiz Gonzaga de Moura Junior, Charles Jean Gomes de Mesquita, Gleydson Cesar de Oliveira Borges
PURPOSE: To create a checklist to evaluate the performance and systematize the gastroenterostomy simulated training. METHODS: Experimental longitudinal study of a quantitative character. The sample consisted of twelve general surgery residents. The training was divided into 5 sessions and consisted of participation in 20 gastroenterostomys in synthetic organs. The training was accompanied by an experienced surgeon who was responsible for the feedback and the anastomoses evaluation...
March 18, 2019: Acta Cirúrgica Brasileira
José de Castro Souza Neto Júnior, Lígia Reis de Moura Estevão, Adriana Aparecida Ferraz, Ricardo Santos Simões, Marcela Gabriela Feitosa Vieira, Joaquim Evêncio-Neto
PURPOSE: To evaluate the healing potential of the Ximenia americana hydroalcoholic extract in 10% cream in excisional wound models in rats. METHODS: Sixty male adults Wistar rats were submitted to skin and subcutaneous tissue surgery in the right and left thoracic regions, divided into three experimental groups: Standard submitted to treatment with only the base vehicle, Treated wounds treated with hydroalcoholic extract of X. americana applied on 10%, Lanette base and Control, untreated wounds...
March 18, 2019: Acta Cirúrgica Brasileira
Nihat Laçin, Bozan Serhat İzol, Ebru Gökalp Özkorkmaz, Buşra Deveci, Mehmet Cudi Tuncer
PURPOSE: To investigate the effects of allopurinol administration on osteoinductive reaction and bone development with graft material. METHODS: Thirty-six Wistar albino rats were divided into 3 groups. In the control group, calvarial bone defect was only created without any treatment. In the Defect + Graft group, allograft treatment was performed by forming 8 mm calvarial bone defect. In the Defect + Graft + Allopurinol group, alloplastic bone graft was placed in the calvarial bone defect and then, allopurinol (50 mg/kg/day) treatment was intraperitoneally applied for 28 days...
March 18, 2019: Acta Cirúrgica Brasileira
Renad Mammadov, Bahadir Suleyman, Durdu Altuner, Elif Demirci, Nihal Cetin, Adnan Yilmaz, Huseyin Baykal, Hilal Alpcan, Emine Akyuz Turumtay, Halis Suleyman
PURPOSE: To investigate the effects of the EtOAc extract of U. longissima which is uninvestigated previously on esophagogastric cancer induced in rats with N-methyl-N-nitro-N-nitrosoguanidin (MNNG). METHODS: The anticancer activity of EtOAc extract of U. longissima was examined in the esophagogastric adenocarcinoma models induced in rats with MNNG. EtOAc extract of U. longissima, 50 and 100 mg/kg oral doses were administered once daily for six months. MNNG induced differentiated and undifferentiated type adenocarcinomas in the esophageal and gastric tissues of rats...
March 18, 2019: Acta Cirúrgica Brasileira
Danusa Neves Somensi, Renan Kleber Costa Teixeira, Daniel Haber Feijó, Karine Drumond Loureiro, André Lopes Valente, Luan Teles Ferreira de Carvalho, Faustino Chaves Calvo, Deivid Ramos Dos Santos, Rui Sergio Monteiro de Barros
PURPOSE: To evaluate if the type of electrode (needle vs. surface) affects the electromyoneurography parameters in rats. METHODS: Twenty male rats were anesthetized, then compound muscle action potential were recorded using a Neuropack S1 MEB- 9400©. All animals were submitted to two electroneuromyography analysis: first with surface electrode and then by needle electrode. We evaluated the latency, amplitude, duration and area of the negative peak of the gastrocnemius and cranial tibial muscles...
March 18, 2019: Acta Cirúrgica Brasileira
Celaleddin Soyalp, Mehmet Nuri Kocak, Ali Ahiskalioglu, Mehmet Aksoy, Canan Atalay, Mehmet Dumlu Aydin, Murteza Cakir, Cagatay Calikoglu, Sevilay Ozmen
PURPOSE: To evaluate whether there is a relationship between renal artery vasospasm related low glomerular density or degeneration and neurogenic lung edema (NLE) following subarachnoid hemorrhage. METHODS: This study was conducted on 26 rabbits. A control group was formed of five animals, a SHAM group of 5 to which saline and a study group (n=16) injected with homologous blood into the sylvian cisterna. Numbers of degenerated axons of renal branches of vagal nerves, atrophic glomerulus numbers and NLE scores were recorded...
March 18, 2019: Acta Cirúrgica Brasileira
Patrícia Madalena San Gregório Guedes, Ariane Zamarioli, Iara Inácio Botega, Raquel Assed Bezerra da Silva, João Paulo Mardegan Issa, Mariana Maloste Butezloff, Yara Terezinha Corrêa Silva Sousa, João Paulo Bianchi Ximenez, José Batista Volpon
PURPOSE: To investigate the effects of dietary restriction on the growth plate and long bone tissue in growing rats. METHODS: Sixty male Wistar rats were randomly assigned to two groups: Control (Con) and Diet-restricted (Res). After weaning, the Res rats were offered 50% of the chow ingested by the control (ad libitum food intake). The animals were subdivided into two subgroups with follow-ups up to 56 or 70 days. After euthanasia, the growth plate of tibias was analyzed by histomorphometry, micro-computed tomography, and mechanical test...
March 18, 2019: Acta Cirúrgica Brasileira
Maria Jose Misael da Silva Morsoleto, Valeria Sella, Paula Machado, Fernando do Bomfim, Maria Helena Fernandes, Fernando Morgado, Gaspar de Jesus Lopes Filho, Helio Plapler
PURPOSE: To analyze aspects of the biomodulating effect of light in biological tissues, bone cells from surgical explants of the femur of rats were irradiated with low intensity laser. METHODS: Bone cells were cultured and irradiated with LASER light (GaAlAs). Growth, cell viability, mineralized matrix formation, total protein dosage, immunostimulatory properties, cytochemical analysis, gene expression of bone proteins were examined using live cell imaging and cell counting by colorimetric assay...
February 28, 2019: Acta Cirúrgica Brasileira
Yang Zhao, Qian Wang, Bin Zang
PURPOSE: To explore the effect of milk fat globule-epidermal growth factor 8 (MFG-E8) on sepsis-induced acute kidney injury (SAKI). METHODS: Male C57BL/6 mice were randomized to control, sham, CLP, CLP+PBS, and CLP+rmMFG-E8 groups. SAKI was induced by cecal ligation and puncture (CLP). Recombinant mouse MFG-E8 (rmMFG-E8) (20 μg/kg) or PBS (vehicle) was administered intraperitoneally. Blood, urine and renal tissue were collected at 24 h after CLP. Blood samples were tested for serum kidney injury biomarker and cytokines...
February 28, 2019: Acta Cirúrgica Brasileira
Yan Li, Fanjun Meng
PURPOSE: To investigate the effects of icariside II on brain tissue oxidative stress and Nrf2/HO-1 expression in rats with cerebral ischemia-reperfusion injury (CIRI). METHODS: One hundred SD rats were randomly divided into sham-operated, model, and 5, 10 and 20 mg/kg icariside II groups, 20 rats in each group. The middle cerebral artery occlusion model (ischemia for 2 h followed by reperfusion for 24 h) was established in the later 4 groups. In later 3 groups, at reperfusion beginning, the rats were intragastrically administrated with 5, 10 and 20 mg/kg icariside II, respectively...
February 28, 2019: Acta Cirúrgica Brasileira
Vanessa Nogueira Lages Braga, Camila de Carvalho Juanes, Hélio de Souza Peres Júnior, José Robson de Sousa, Bruno Coêlho Cavalcanti, Francisco Vagnaldo Fechine Jamacaru, Telma Leda Gomes de Lemos, Conceição Aparecida Dornelas
PURPOSE: To evaluate red propolis, gum arabic and L-lysine activity on colorectal preneoplastic lesions induced by azoxymethane (AOM). METHODS: The study featured 4 control groups (I-IV) and 4 experimental groups (V-VIII), totaling 48 rats. Once a week for 2 weeks, animals on control groups received saline, while animals in experimental groups received azoxymethane (15 mg/kg i.p.). The follow up along 16 weeks included daily oral gavage to administer water (I and V), L-lysine (150 mg/kg)(II and VI), própolis (100mg/5ml/kg)(III and VII), or gum arabic (5ml/kg)(IV and VIII)...
February 28, 2019: Acta Cirúrgica Brasileira
Milton Rigoberto Fonseca Quispe, Wilson Salgado Júnior
PURPOSE: To compare open Lichtenstein repair and laparoscopic transabdominal preperitoneal (TAPP) repair to treat primary unilateral hernia, regarding systemic inflammatory response, postoperative pain, and complications. METHODS: A non-randomized prospective cohort study, with the preoperative and postoperative (24 hours) collection of blood samples for C reactive protein (CRP), interleukin 6 (IL-6), leukocyte and neutrophil analysis. Visual Analog Scale (VAS) was used to quantify the level of pain, and the operative time was correlated with the inflammatory response...
February 28, 2019: Acta Cirúrgica Brasileira
Marcos Fiuza de Carvalho, Thuliermes Lopes Pamplona, Márcio Alencar Barreira, Francisco Vagnaldo Jacuru Fechine, Lúcio Flávio Gonzaga-Silva, Ricardo Reges Maia de Oliveira
PURPOSE: To evaluate the impact of the combination of BRL 37344 and tadalafil (TDF) on the reduction of overactive bladder (OB) symptoms. METHODS: Thirty mice were randomized into 5 groups (G) of 6 animals each. L-NAME was used to induce DO. G1: Control; G2: L-NAME; G3: L-NAME + TDF; G4: L-NAME + BRL 37344; G5: L-NAME + TDF + BRL 37344. After 30 days of treatment, the animals were submitted to cystometry to evaluate non-voiding contractions (NVC), threshold pressure (TP), baseline pressure (BP), frequency of micturition (FM) and threshold volume (TV)...
February 28, 2019: Acta Cirúrgica Brasileira
Guangming Zhang, Guanghua Cui, Shuangxi Tong, Qingxian Cao
PURPOSE: To investigate the protective effects of salvianolic acid A (SAA) on renal damage in rats with chronic renal failure (CRF). METHODS: The five-sixth nephrectomy model of CRF was successfully established in group CRF (10 rats) and group CRF+SAA (10 rats). Ten rats were selected as sham-operated group (group S), in which only the capsules of both kidneys were removed. The rats in group CRF+SAA were intragastrically administrated with 10 mg/kg SAA for 8 weeks...
February 28, 2019: Acta Cirúrgica Brasileira
George Gonçalves Dos Santos, Luisa Queiroz Vasconcelos, Suelen Cristina da Silva Poy, Renata Dos Santos Almeida, Aryon de Almeida Barbosa Júnior, Silvia Rachel de Albuquerque Santos, Alexandre Malta Rossi, Fúlvio Borges Miguel, Fabiana Paim Rosa
PURPOSE: To analyze, histomorphologically, the influence of the geometry of nanostructured hydroxyapatite and alginate (HAn/Alg) composites in the initial phase of the bone repair. METHODS: Fifteen rats were distributed to three groups: MiHA - bone defect filled with HAn/Alg microspheres; GrHA - bone defect filled with HAn/Alg granules; and DV - empty bone defect; evaluated after 15 days postoperatively. The experimental surgical model was the critical bone defect, ≅8...
February 28, 2019: Acta Cirúrgica Brasileira
Cristina Pires Camargo, Renan Dias Frassei, Daniel Imbassahy de Sá Bittencourt Camara E Silva, Robert Zawadzki Pfann, Luiza de Campos Moreira da Silva, Julio Morais-Besteiro, Rolf Gemperli
PURPOSE: To evaluate the hyaluronic acid (HA) inflammatory reaction, fibroblasts, fibrosis and duration of effect in the dorsal region of tobacco-exposed rats. METHODS: Ten Wistar rats were divided into two groups: tobacco-exposed-group (TEG;n=5) and air-control-group (CG;n=5). The TEG animals were tobacco-exposed twice a day, 30-minutes/session, during 60 days. After this period, all animals received 0.1 mL HA subcutaneous injection in the dorsal area. The volume of HA was measured immediately after HA injection and weekly using a hand-caliper in nine weeks...
February 28, 2019: Acta Cirúrgica Brasileira
Changxia Dong, Peng Liu, Huaizhou Wang, Mei Dong, Guangxin Li, Yuanbin Li
PURPOSE: To investigated the effects of ginsenoside Rb1 on diabetic retinopathy in streptozotocin-induced diabetic rats. METHODS: Diabetes was induced by a single intraperitoneal injection of streptozotocin (80 mg/kg) in male Wistar rats. Ginsenoside Rb1 (20, 40 mg/kg) was injected (i.p.) once a day for 4 weeks. Then, using fundus photography, the diameter and vascular permeability of retinal vessels were investigated. Retinal histopathology was undertaken. Contents of malondialdehyde (MDA) and glutathione (GSH) in retinas were assayed...
February 28, 2019: Acta Cirúrgica Brasileira
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