Hongwen Li, Jing Jiang, Haiying Kong, Wenbo Wu, Xiaomin Shao, Shuqi Qiu, Xianhai Zeng, Qinghong Zhong, Xinhui Yao, Xiantao Zeng, Lingshan Gou, Jian Xu
Mesenchymal stem cells (MSCs) have shown great potential as important therapeutic tools for dental pulp tissue engineering, with the maintenance and enhancement of their stemness being crucial for successful therapeutic application in vivo and three-dimensional (3D) spheroid formation considered a reliable technique for enhancing their pluripotency. Human exfoliated deciduous tooth stem cells (SHED) were cultured in a low attachment plate to form aggregates for five days. Then, the resulting spheroids were analyzed for pluripotent marker expression, paracrine secretory function, proliferation, signaling pathways involved, and distribution of key proteins within the spheroids...
November 2023: Journal of Clinical Pediatric Dentistry