journal
https://read.qxmd.com/read/38164963/temperature-adaptation-patterns-in-chinese-cattle-revealed-by-trpm2-gene-mutation-analysis
#21
JOURNAL ARTICLE
Dekai Liu, Yifan Yang, Zhefu Chen, Yijie Fan, Jianyong Liu, Yibing Xu, Zulfiqar Ahmed, Jicai Zhang, Fuqiang Li, Xingshan Qi, Weiru Song, Kaixia Zhu, Jiangcai Gongque, Guomei Li, Bizhi Huang, Chuzhao Lei
Cattle are sensitive to temperature fluctuations but adapt well to inclement weather conditions. When environmental temperatures exceed specific thresholds, heat stress becomes a critical concern for cattle. The TRPM2 gene, which resides on cattle chromosome 1 encodes a TRP channel protein, holding a unique capacity to sense temperature changes and facilitate rapid response to avoid heat stress. Here, we utilized the Bovine Genome Variation Database (BGVD) (https://animal.omics.pro/code/index.php/BosVar), and identified a missense mutation site, c...
January 2, 2024: Animal Biotechnology
https://read.qxmd.com/read/38526422/effects-of-dietary-supplementation-of-grape-seed-extract-in-comparison-with-excessive-level-of-vitamin-e-on-growth-performance-and-antioxidant-function-of-broilers
#22
JOURNAL ARTICLE
Man Wang, Zongze He, Zhaolong Xiong, Hongwei Liu, Xiang Zhou, Jian He
The present study was carried out to evaluate the effects of dietary vitamin E (VE) or grape seed extract (GSE) on the growth performance and antioxidant function of broilers. Two hundred sixteen broiler chicks were randomly assigned to 3 diets: diet supplemented with oxidized rice bran oil (CN group), CN group with 25 mg/kg VE or 100 mg/kg GSE. Dietary VE or GSE improved the growth performance, reverted the disturbed levels of liver antioxidant enzymes, and reduced liver damage of broilers fed oxidized rice bran oil...
November 2024: Animal Biotechnology
https://read.qxmd.com/read/38519440/microbial-transcriptome-integrative-analysis-of-heat-stress-effects-on-amino-acid-metabolism-and-lipid-peroxidation-in-poultry-jejunum
#23
JOURNAL ARTICLE
Young-Jun Seo, Chiwoong Lim, Byeonghwi Lim, Jun-Mo Kim
Despite the significant threat of heat stress to livestock animals, only a few studies have considered the potential relationship between broiler chickens and their microbiota. Therefore, this study examined microbial modifications, transcriptional changes and host-microbiome interactions using a predicted metabolome data-based approach to understand the impact of heat stress on poultry. After the analysis, the host functional enrichment analysis revealed that pathways related to lipid and protein metabolism were elevated under heat stress conditions...
November 2024: Animal Biotechnology
https://read.qxmd.com/read/38520296/toll-like-receptor-4-tlr4-gene-polymorphism-and-its-association-with-somatic-cell-score-and-milk-production-traits-in-indian-dromedary-camels
#24
JOURNAL ARTICLE
Seema Bishnoi, Basanti Jyotsana, Virendra Kumar, Ved Prakash, Rakesh Ranjan, Shirish Dadarao Narnaware, Urmila Pannu
Our study aimed to explore the genetic variation in the Toll-like receptor 4 (TLR4) gene and establish its association with somatic cell score (SCS) and milk production traits in four Indian camel breeds namely Bikaneri, Kachchhi, Jaisalmeri and Mewari. TLR4 gene fragment of 573 bp spanning 5' UTR, exon-1 and partial intron-1 region was amplified and genotyped using the PCR-sequence based typing method. Only one SNP located at position C472T was identified. Genotyping revealed two alleles (C and T) and three genotypes: CC, CT and TT...
March 23, 2024: Animal Biotechnology
https://read.qxmd.com/read/38157229/effect-of-dhcr7-on-adipocyte-differentiation-in-goats
#25
JOURNAL ARTICLE
Zhibin Li, Tingting Hu, Ruiwen Li, Jinlan Li, Youli Wang, Yanyan Li, Yaqiu Lin, Yong Wang, Xing Jiani
Cholesterol is regarded as a signaling molecule in regulating the metabolism and function of fat cells, in which 7-Dehydrocholesterol reductase (DHCR7) is a key enzyme that catalyzes the conversion of 7-dehydrocholesterol to cholesterol, however, the exact function of DHCR7 in goat adipocytes remains unknown. Here, the effect of DHCR7 on the formation of subcutaneous and intramuscular fat in goats was investigated in vitro , and the result indicated that the mRNA level of DHCR7 showed a gradual downward trend in subcutaneous adipogenesis, but an opposite trend in intramuscular adipogenesis...
December 29, 2023: Animal Biotechnology
https://read.qxmd.com/read/38149679/identification-of-ago2-and-plec-genes-polymorphisms-in-hu-sheep-and-their-relationship-with-body-size-traits
#26
JOURNAL ARTICLE
Jia Liu, Wenxin Zheng, Weimin Wang, Xiaobin Yang, Yongliang Huang, Panpan Cui, Zongwu Ma, Xiwen Zeng, Rui Zhai, Xiuxiu Weng, Weiwei Wu, Xiaoxue Zhang
The body size traits are major traits in livestock, which intuitively displays the development of the animal's bones and muscles. This study used PCR amplification, Sanger sequencing, KASPar genotyping, and quantitative real-time reverse transcription PCR (qRT-PCR) to analyze the Single-nucleotide polymorphism and expression characteristics of Argonaute RISC catalytic component 2 ( AGO2 ) and Plectin ( PLEC ) genes in Hu sheep. Two intron mutations were found in Hu sheep, which were AGO2 g.51700 A > C and PLEC g...
December 27, 2023: Animal Biotechnology
https://read.qxmd.com/read/38147885/rna-seq-analysis-of-small-intestine-transcriptional-changes-induced-by-starvation-stress-in-piglets
#27
JOURNAL ARTICLE
Yijia Ma, Tong Guo, Jianqin Ding, Zhiling Dong, Yifei Ren, Chang Lu, Yan Zhao, Xiaohong Guo, Guoqing Cao, Bugao Li, Pengfei Gao
Piglets may experience a variety of stress injuries, but the molecular regulatory mechanisms underlying these injuries are not well understood. In this study, we analysed the ileum of Large White (LW) and Mashen (MS) piglets at different times of starvation using chemical staining and transcriptome analysis. The intestinal barrier of piglets was damaged after starvation stress, but the intestinal antistress ability of MS piglets was stronger than LW piglets. A total of 8021 differentially expressed genes (DEGs) were identified in two breeds...
December 26, 2023: Animal Biotechnology
https://read.qxmd.com/read/38142270/morpometric-and-molecular-characterization-of-surguli-goat-through-co1-gene-in-district-kohat
#28
JOURNAL ARTICLE
Muhammad Munir Khan, Syed Muhammad Suhail, Hafiz Abdul Majid, Ijaz Ahmad, Umer Sadique, Rajwali Khan, Iftikhar Ahmad, Asim Ijaz, Khalid Khan, Farhad Ali, Muhammad Saeed Khan, Ahmed A El-Mansi
The present study was designed with the aim to study morphometric characterization as well as phylogeny and diversity of the local Surguli goat at their breeding tract district Kohat through mitochondrial DNA region, i.e., Cytochrome C Oxidase Subunit One (CO1) gene. Morphometric data and blood samples were collected from thirty (30) pure goats. Morphometric analysis showed that sex had significant effect ( p  < 0.05) on body weight, body length, hearth girth and horn length while no significant effect ( p  > 0...
December 23, 2023: Animal Biotechnology
https://read.qxmd.com/read/38141161/transcriptome-sequencing-analysis-of-bovine-mammary-epithelial-cells-induced-by-lipopolysaccharide
#29
REVIEW
Jingjing Liu, Yingkui Gao, Xing Zhang, Zhonghua Hao, Huaqiang Zhang, Rong Gui, Fang Liu, Chao Tong, Xuebing Wang
Mastitis in cows is caused by the inflammation of the mammary glands due to an infection by external pathogenic bacteria. Mammary gland epithelial cells, which are in direct contact with the external environment, are responsible for the first line of defense of the mammary gland against pathogenic bacteria, playing an essential role in immune defense. To investigate the mechanism of bovine mammary epithelial cells in the inflammatory process, we treated the cells with LPS for 12 hours and analyzed the changes in mRNA by transcriptome sequencing...
December 23, 2023: Animal Biotechnology
https://read.qxmd.com/read/38100547/effect-of-nk-lysin-peptides-on-bacterial-growth-mic-antimicrobial-resistance-and-viral-activities
#30
JOURNAL ARTICLE
Haitham A Yacoub, Maged Mostafa Mahmoud, Ahmed M Al-Hejin, Turki S Abujamel, Shams Tabrez, Sherif Abd-Elmaksoud
NK-lysins from chicken, bovine and human are used as antiviral and antibacterial agents. Gram-negative and gram-positive microorganisms, including Streptococcus pyogenes, Streptococcus mutans, Escherichia coli, Pseudomonas aeruginosa, Klebsiella oxytoca, Shigella sonnei, Klebsiella pneumoniae and Salmonella typhimurium , are susceptible to NK-lysin treatment. The presence of dominant TEM-1 gene was noted in all untreated and treated bacteria, while TOHO-1 gene was absent in all bacteria. Importantly, β-lactamase genes CTX-M-1, CTX-M-8, and CTX-M-9 genes were detected in untreated bacterial strains; however, none of these were found in any bacterial strains following treatment with NK-lysin peptides...
December 15, 2023: Animal Biotechnology
https://read.qxmd.com/read/38088885/genome-wide-selection-signatures-address-trait-specific-candidate-genes-in-cattle-indigenous-to-arid-regions-of-india
#31
JOURNAL ARTICLE
Nidhi Sukhija, Anoop Anand Malik, Joel M Devadasan, Aishwarya Dash, Kangabam Bidyalaxmi, D Ravi Kumar, M Kousalaya Devi, Anjali Choudhary, K K Kanaka, Rekha Sharma, Shashi Bhushan Tripathi, Saket Kumar Niranjan, Jayakumar Sivalingam, Archana Verma
The peculiarity of Indian cattle lies in milk quality, resistance to diseases and stressors as well as adaptability. The investigation addressed selection signatures in Gir and Tharparkar cattle, belonging to arid ecotypes of India. Double digest restriction-site associated DNA sequencing (ddRAD-seq) yielded nearly 26 million high-quality reads from unrelated seven Gir and seven Tharparkar cows. In all, 19,127 high-quality SNPs were processed for selection signature analysis. An approach involving within-population composite likelihood ratio (CLR) statistics and between-population F ST statistics was used to capture selection signatures within and between the breeds, respectively...
December 13, 2023: Animal Biotechnology
https://read.qxmd.com/read/38085574/effects-of-nanoselenium-supplementation-on-lactation-performance-nutrient-digestion-and-mammary-gland-development-in-dairy-cows
#32
JOURNAL ARTICLE
Yapeng Liu, Jing Zhang, Lijun Bu, Wenjie Huo, Caixia Pei, Qiang Liu
The objective of this experiment was to evaluate the influence of nanoselenium (NANO-Se) addition on milk production, milk fatty acid synthesis, the development and metabolism regulation of mammary gland in dairy cows. Forty-eight Holstein dairy cows averaging 720 ± 16.8 kg of body weight, 66.9 ± 3.84 d in milk (dry matter intake [DIM]) and 35.2 ± 1.66 kg/d of milk production were divided into four treatments blocked by DIM and milk yields...
December 12, 2023: Animal Biotechnology
https://read.qxmd.com/read/38347693/development-of-real-time-rt-pcr-systems-for-detection-and-quantitation-of-bovine-enteric-viral-pathogens
#33
JOURNAL ARTICLE
Monika Punia, Sushila Maan, Kanisht Batra, Deepika Chaudhary, Bhanita Devi, Aman Kumar, Suresh Kumar Gahlawat, Narender Singh Maan
The enteric viruses in animals are responsible for severe and devastating losses to the livestock owners with a profound negative impact on animal, health, welfare, and productivity. These viruses are usually transmitted via the feco-oral route and primarily infect the digestive tract of the humans, bovines and different mammals as well as birds. Some of the important enteric viruses in ruminants are: Rotavirus A (RVA), Peste des petits virus (PPRV), Norovirus (NV), Bovine corona virus (BoCV) and Bluetongue virus (BTV)...
December 2023: Animal Biotechnology
https://read.qxmd.com/read/37764644/immunolocalization-of-antibacterial-peptide-s100a7-in-mastitis-goat-mammary-gland-and-lipopolysaccharide-induces-the-expression-and-secretion-of-s100a7-in-goat-mammary-gland-epithelial-cells-via-tlr4-nf%C3%AE%C2%BAb-signal-pathway
#34
JOURNAL ARTICLE
Yutong Yan, Kunyuan Zhu, Mingzhen Fan, Wenjing Wan, Xiaoe Zhao, Menghao Pan, Baohua Ma, Qiang Wei
The antimicrobial peptide S100A7, with antimicrobial activities for a broad spectrum of bacteria, has attracted more and more attention for the prevention and treatment of mastitis. However, there is little information about the expression and regulation mechanism of S100A7 in mastitis goats. This study revealed that S100A7 was mainly expressed in the stratified squamous epithelium of teat skin and streak canal, and S100A7 was present weakly in the healthy goat alveolus yet densely in the mastitis goat collapsed alveolus...
December 2023: Animal Biotechnology
https://read.qxmd.com/read/38032316/candidate-circrnas-related-to-skeletal-muscle-development-in-dazu-black-goats
#35
JOURNAL ARTICLE
Chengli Liu, Pu Yang, Xiao Wang, Baiju Xiang, Guangxin E, Yongfu Huang
Circular RNA (CircRNA), as a classical noncoding RNA, has been proven to regulate skeletal muscle development (SMD). However, the molecular genetic basis of circRNA regulation in muscle cells remains unclear. In this study, the expression patterns of circRNAs in the longissimus dorsi muscle at embryonic day 75 and postnatal day 1 in DBGs were investigated to identify the key circRNAs that play an important role in SMD in goats. A total of 140 significantly and differentially expressed circRNAs (DEcircRNAs) were identified among the groups at different developmental stages...
November 30, 2023: Animal Biotechnology
https://read.qxmd.com/read/38006583/-orychophragmus-violaceus-and-or-chicory-forage-affects-performance-egg-quality-sensory-evaluation-and-antioxidative-properties-in-native-laying-hens
#36
JOURNAL ARTICLE
W Y Zhao, Q Q Zhang, Y F Zhao, C Chang, X Wang, A L Geng
Orychophragmus violaceus (OV) and chicory ( Cichorium intybus L., CC) can be used as fresh or dry forage for animals. To determine whether OV and/or CC have beneficial effects on performance and egg quality, a total of 1212 28-wk-old Beijing You Chicken (BYC) laying hens with similar performance were randomly allocated to 4 groups with 3 replicate pens per group, and 101 birds per pen. The birds were fed a basal diet (control), the basal diet + OV (3.507 kg/d/pen), the basal diet + CC (2...
November 25, 2023: Animal Biotechnology
https://read.qxmd.com/read/38006579/analysis-of-oct4-and-pgp9-5-gene-expression-in-prenatal-and-postnatal-buffalo-bubalus-bubalis-testes
#37
JOURNAL ARTICLE
Ashritha Q Carlo, Devendra Pathak, Ratan K Choudhary, Opinder Singh, Neelam Bansal
This study aimed to investigate and characterize the spermatogonial stem cells (SSCs) in buffaloes at different stages of development, including prenatal, neonatal, prepubertal, and adult testes. We sought a comprehensive understanding of these cells through a combination of histological, immunohistochemical, and ultrastructural analyses. Specifically, we examined changes in the expression of two potential SSC markers, OCT4 and PGP9.5, using immunohistochemistry. Additionally, we conducted a real-time quantitative polymerase chain reaction (RT-qPCR) to assess the relative gene expression of OCT4 and PGP9...
November 25, 2023: Animal Biotechnology
https://read.qxmd.com/read/38006572/comparative-transcriptome-profile-analysis-of-granulosa-cells-from-porcine-ovarian-follicles-during-early-atresia
#38
JOURNAL ARTICLE
Jinbi Zhang, Xinxin Qin, Caixia Wang, Yuge Zhang, Yizhe Dou, Shiyong Xu, Jingge Liu, Zengxiang Pan
At various stages of ovarian follicular development, more than 99% of follicles will be eliminated through a degenerative process called atresia. The regulatory mechanisms of atresia have been elucidated to some extent, involving hormones, growth factors, cytokines, and other factors. However, the stimuli initiating atresia in follicular granulosa cells remain unknown. In this study, we isolated the granulosa cells from porcine ovarian follicles (3-5 mm diameter) divided into healthy follicles (HFs) and early atretic follicles (EAFs)...
November 25, 2023: Animal Biotechnology
https://read.qxmd.com/read/38006247/whole-genome-resequencing-revealed-genomic-variants-and-functional-pathways-related-to-adaptation-in-indian-yak-populations
#39
JOURNAL ARTICLE
Amod Kumar, Mahesh Dige, Saket Kumar Niranjan, Sonika Ahlawat, Reena Arora, Aneet Kour, Ramesh Kumar Vijh
The present study aims to identify genomic variants through a whole genome sequencing (WGS) approach and uncover biological pathways associated with adaptation and fitness in Indian yak populations. A total of 30 samples (10 from each population) were included from Arunachali, Himachali and Ladakhi yak populations. WGS analysis revealed a total of 32171644, 27260825, and 32632460 SNPs and 4865254, 4429941, and 4847513 Indels in the Arunachali, Himachali, and Ladakhi yaks, respectively. Genes such as RYR2 , SYNE2 , BOLA , HF1 , and the novel transcript ENSBGRG00000011079 were found to have the maximum number of high impact variants in all three yak populations, and might play a major role in local adaptation...
November 25, 2023: Animal Biotechnology
https://read.qxmd.com/read/37982395/condition-optimization-for-electroporation-transfection-in-horse-skeletal-muscle-satellite-cells
#40
JOURNAL ARTICLE
Tseweendolmaa Ulaangerel, Minna Yi, Undarmaa Budsuren, Yingchao Shen, Hong Ren, Bold Demuul, Dongyi Bai, Dulguun Dorjgotov, Gantulga Davaakhuu, Tuyatsetseg Jambal, Manglai Dugarjav, Gerelchimeg Bou
Satellite cells are an important cellular model for studying muscle growth and development and mammalian locomotion-related molecular mechanisms. In this study, we investigated the effects of voltage, pulse duration, and DNA dosage on horse skeletal muscle satellite cells' electroporation transfection efficiency using the eukaryotic expression plasmid Td Tomato-C1 (5.5 kb) encoding the red fluorescent protein gene mainly based on fluorescence-positive cell rate and cell survival rate. By comparison of different voltages, pulse durations, and DNA doses, horse skeletal muscle satellite cells have nearly 80% transfection efficiency under the condition of voltage 120 V, DNA dosage 7 µg/ml, and pulse duration 30 ms...
November 20, 2023: Animal Biotechnology
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