Ricarda C Steffens, Paul Folda, Nikole L Fendler, Miriam Höhn, Katharina Bücher-Schossau, Susanne Kempter, Nicole L Snyder, Laura Hartmann, Ernst Wagner, Simone Berger
A cationic, dendrimer-like oligo(aminoamide) carrier with four-arm topology based on succinoyl tetraethylene pentamine and histidines, cysteines, and N -terminal azido-lysines was screened for plasmid DNA delivery on various cell lines. The incorporated azides allow modification with various shielding agents of different polyethylene glycol (PEG) lengths and/or different ligands by copper-free click reaction, either before or after polyplex formation. Prefunctionalization was found to be advantageous over postfunctionalization in terms of nanoparticle formation, stability, and efficacy...
March 5, 2024: Bioconjugate Chemistry