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Anna Lindblad, Katarina Persson, Isak Demirel
The NLRP3 inflammasome, IL-1β release and pyroptosis (cell lysis) have recently been proposed to be essential for the progression of urinary tract infection (UTI) and elimination of intracellular bacterial niches. However, the effects of IL-1R antagonist (IL-1RA) on immune responses during UTI, except for its ability to disrupt IL-1β signalling, are not well understood. The aim of this study was to investigate the role of IL-1RA in UPEC colonization of bladder epithelial cells and the subsequent host inflammatory response...
July 9, 2019: Cytokine
Victor V S Albuquerque, Nathella Pavan Kumar, Kiyoshi F Fukutani, Beatriz Vasconcelos, Maria B Arriaga, Paulo S Silveira-Mattos, Subash Babu, Bruno B Andrade
The immune profile associated with distinct clinical forms of tuberculosis (TB) has been extensively described for adult populations. Nevertheless, studies describing immune determinants of pulmonary or extrapulmonary TB (PTB or EPTB, respectively) in children are scarce. Here, we retrospectively assessed plasma levels of several mediators of inflammation in age and sex-matched children from South India presenting with PTB (n = 14) or EPTB (n = 22) as well as uninfected healthy controls (n = 19) to identify biomarkers that could accurately distinguish different TB clinical forms...
July 9, 2019: Cytokine
Alicja E Grzegorzewska, Monika K Świderska, Hanna Winnicka, Leszek Niepolski, Maciej Bura, Małgorzata Łagiedo-Żelazowska, Paweł P Jagodziński
Responsiveness to the hepatitis B virus (HBV) vaccination in hemodialysis (HD) patients who had been exposed to the hepatitis E virus (HEV) and persistently generate antibodies against HEV remains unknown. Interferon (IFN)-λ3 positively correlates with the surface HBV antibodies (anti-HBs) in both healthy and HD subjects. We aimed to show whether HD patients differ in circulating IFN-λ3 and vaccine-induced anti-HBs titers concerning natural HEV immunization. HBV/HCV negative HD patients (31 HEV IgG positive, 45 HEV negative), HBV vaccinated and receiving booster doses as needed, had been tested for anti-HBs titers (CMIA) and IFN-λ3 concentrations (ELISA) in the blood collected before a dialysis session...
July 3, 2019: Cytokine
Hidemi Hashimoto, Mikio Watanabe, Naoya Inoue, Nachi Hirai, Emi Haga, Riku Kinoshita, Yoh Hidaka, Yoshinori Iwatani
The intractability of Graves' disease (GD) and the severity of Hashimoto's disease (HD) vary among patients. Both genetic and environmental factors may be associated with their prognoses. To clarify the role of methylation of the IFNG gene in the pathogenesis and prognosis of (AITDs), we examined interferon gamma (IFNG) methylation levels at various CpG sites and genotyped IFNG +874 A/T and +2109 C/T polymorphisms. We analyzed methylation 59 patients with HD, 57 patients with GD and 26 healthy volunteers by pyrosequencing...
July 3, 2019: Cytokine
Alan Valaperti, Pascal Bezel, Maya Vonow-Eisenring, Daniel Franzen, Urs C Steiner
Measurement of cytokines in peripheral blood and bronchoalveolar lavage fluid (BALF) is a useful method to assess human immune responses in a large range of pulmonary diseases. One of the major pre-analytical challenges of cytokine analysis is the quality and stability of cytokines in the timeframe between sample collection and the separation of supernatant from cells. To evaluate if the method of storage may affect cytokine quantification, whole blood and BALF were collected, aliquoted, and left at room temperature (RT) to be processed at different time points...
July 2, 2019: Cytokine
A Z Szóstek-Mioduchowska, A Baclawska, K Okuda, D J Skarzynski
Equine endometrosis (endometrial fibrosis) is a degenerative chronic process that occurs in the uterus of the mare and disturbs proper endometrial function. Fibrosis is attributed to excessive deposition of extracellular matrix (ECM) components. The turnover of ECM is mediated by matrix metallopeptidases (MMP). Previously, it was shown that cytokines modulate MMP expression in other tissues and may regulate fibrosis indirectly by attracting inflammatory cells to the site of inflammation and directly on various tissues...
June 29, 2019: Cytokine
Jun Guo, Yanfei Li, Ruqian Zhao, Xiaojing Yang
Humans and animals frequently experience dysmetabolism induced by inflammation. Zinc-α2-glycoprotein (ZAG), a newly identified adipokine, is potentially involved in lipid metabolism. Our previous study revealed that the ZAG content increased after lipopolysaccharide (LPS) treatment. To clarify ZAG's possible effects on inflammatory responses and lipid metabolism, we used gene overexpression and knockout mice as models to investigate the function of ZAG during inflammation. The results showed that LPS increased plasma triglyceride, non-esterified fatty acid and hepatic triglyceride, while ZAG overexpression decreased these effects...
June 28, 2019: Cytokine
Mohammad Salimi Asl, Ali Ahmadi, Jafar Salimian, Sepideh Shohani, Sadegh Azimzadeh Jamalkandi, Mostafa Ghanei
BACKGROUND AND OBJECTIVE: TNF-α -308 G/A variant is recognized to play an important role in the pathogenesis of chronic obstructive pulmonary disease (COPD). Although many studies have investigated the association of TNF-α-308 and COPD risk, a deep understanding of this association is lacking due to small subjects sizes and insufficiently study designs among different investigations. In this study, a systematic review and meta-analysis was performed based on published reports on the association of TNF-α and COPD...
June 28, 2019: Cytokine
Surinder Safaya, Mohammed Alfarhan, Ahmed Sulaiman, Abdulla Alsulaiman, Amein Al-Ali
BACKGROUND: Sickle cell anemia (SCA), a disorder with an important inflammatory component, where vasoocclusion is major contributor to the disease pathophysiology. Pro-inflammatory cytokines play an important regulatory role in the process of inflammation. We investigated the expression TL1A/DR3/DcR3 cytokine signaling pathway in peripheral blood mononuclear cells (PBMC) and their corresponding plasma levels in SCA subjects who presented with acute painful episodes. MATERIALS AND METHODS: PBMC were isolated from the blood of SCA subjects and normal healthy controls...
June 27, 2019: Cytokine
E Ramos-Martínez, J Rojas-Serrano, O García-Hernández, F J García-Vázquez, W A Andrade, G Avila, L Salinas-Pasquier, M R López-Vancell
BACKGROUND: Cancer is a high-impact disease throughout the world. A negative correlation has been established between the development of cancer and the Th2 immune response. Infection by helminth parasites is characterized by the induction of a strong and long-lasting Th2 response. The aim of this work was to evaluate the effect of the immune response induced by the infection with the helminth Hymenolepis nana, on the tumorigenesis induced by dimethylbenz-anthracene (DMBA) in mice. METHODOLOGY: Four different groups of 14 female BALB/c mice were formed; Group A, dimethyl sulfoxide (DMSO) (vehicle) was administered cutaneously, Group B infected with H...
June 27, 2019: Cytokine
Megumi Funakoshi-Tago, Rina Tsuruya, Fumihito Ueda, Aki Ishihara, Tadashi Kasahara, Hiroomi Tamura, Kenji Tago
In the majority of myeloproliferative neoplasms (MPNs) patients, a point mutation, V617F has been found in Janus kinase 2 (JAK2) gene, and this JAK2 mutant provoked aberrant signaling pathway. In the current study, we found that suppressor of cytokine signaling proteins 3 (SOCS3) possessed the tumor suppressive activity against the JAK2 V617F mutant-provoked cellular transformation. The knockdown of SOCS3 increased the expression level of the JAK2 V617F mutant, which enhanced the activation of signaling mediators, including signal transducer and activator of transcription 3 and 5 (STAT3, STAT5) and extracellular signal-regulated kinase (ERK), and also increased of the proliferation rate and tumorigenesis activity of Ba/F3 cells expressing the JAK2 V617F mutant and erythropoietin receptor (EpoR)...
June 27, 2019: Cytokine
Yaqing He, Zhuoying Feng, Wei Wang, Ying Chen, Jinquan Cheng, Jun Meng, Hong Yang, Yujie Wang, Xiangjie Yao, Qianjin Feng, Long Chen, Hailong Zhang, Maggie H T Wang, Benny C Y Zee, Xin Wang, Ming-Liang He
OBJECTIVE: New clinical indicators are urgently needed for predicting the progression and complications of hand-foot-and-mouth disease (HFMD) caused by EV-A71 infections. MATERIALS AND METHODS: Serum specimens from 132 EV-A71 HFMD patients and 73 health children were collected during 2012-2014 in Shenzhen, China. The specific cytokines/chemokines were detected with a 274-human cytokine antibody array, followed by a 38-inflammation cytokine array, and further validated by ELISA...
June 27, 2019: Cytokine
Bailing Tian, Chunshu Yang, Jianing Wang, Xiaoyu Hou, Shan Zhao, Yujia Li, Pingting Yang
OBJECTIVE: Brain-derived neurotrophic factor (BDNF) has been reported to be involved in the pathogenesis of autoimmune diseases and tyrosine kinase B (TrkB) is the specific receptor for BDNF. Our aim in this study was to investigate serum BDNF level and TrkB expression on peripheral blood T cell surface in patients with systemic lupus erythematosus (SLE) and explore potential relationship between serum BDNF and SLE. METHODS: Samples from fifty SLE patients and thirty healthy controls were evaluated...
June 27, 2019: Cytokine
Brett R Vanderwerff, Kevin J Church, Leen H Kawas, Joseph W Harding
Pancreatic cancer is an aggressive disease with a poor prognosis for which current standard chemotherapeutic treatments offer little survival benefit. Receptor tyrosine kinases (RTK)s have garnered interest as therapeutic targets to augment or replace standard chemotherapeutic treatments because of their ability to promote cell growth, migration, and survival in various cancers. Met and Ron, which are homologous RTKs activated by the ligands hepatocyte growth factor (HGF) and macrophage stimulating protein (MSP), respectively, are over-activated and display synergistic malignant effects in several cancers...
June 26, 2019: Cytokine
Amene Saghazadeh, Bahar Ataeinia, Kimia Keynejad, Amirhussein Abdolalizadeh, Armin Hirbod-Mobarakeh, Nima Rezaei
BACKGROUND: In the search for the causes of autism spectrum disorders (ASD), inflammatory markers have emerged as potential candidates. The present meta-analysis was performed on studies examining circulating concentrations of anti-inflammatory cytokines in people with ASD compared with control subjects without ASD. METHODS: We identified potentially eligible studies by systematically searching electronic databases from inception to February 2018. RESULTS: Twenty-five studies with a total of 1754 participants (1022 patients with ASD and 732 control subjects) were included in the mate-analysis; 4 for interferon (IFN)-α, 9 for interleukin (IL)-1 receptor antagonist (Ra), 9 for IL-4, 6 for IL-5, 3 for IL-9, 14 for IL-10, 7 for IL-13, and 6 for transforming growth factor (TGF)-β...
June 19, 2019: Cytokine
Alireza Milajerdi, Bagher Larijani, Ahmad Esmaillzadeh
BACKGROUND: No earlier study summarized findings on the effect of statins on inflammatory biomarkers in apparently healthy individuals or those with chronic diseases. This study was done to systematically review earlier publications on the effect of statins on serum concentrations of C-reactive protein (CRP) and Interleukin-6 (IL-6) in apparently healthy individuals or those with chronic diseases. METHODS: We searched relevant publications published up to December 2018 in PubMed, MEDLINE, SCOPUS, EMBASE, and Google Scholar databases...
June 19, 2019: Cytokine
Tegan Hartmann, Frank Marino, Rob Duffield
This study examined the acute effects of combined tobacco smoking and exercise on immune-inflammatory responses in smoker populations with shorter or longer smoking history. The cohort comprised 14 young male adult (YSM) and 12 middle-aged (MSM) male active cigarette smokers matched for aerobic fitness and smoking behavior. Following an initial familiarization and baseline testing session, participants completed a smoking and exercise protocol. This protocol involved the inhalation of two cigarettes (12 mg tar, 1 mg nicotine) within 15 min, and following a 10 min recovery period, 40 min of cycling at 50% peak aerobic workload...
June 19, 2019: Cytokine
Natalia Da Ponte Leguizamón, Elisandra Marcia Rodrigues, Michel Leandro de Campos, Andressa Vilas Boas Nogueira, Kennia Scapin Viola, Vanessa Karine Schneider, Daniela Morilha Neo-Justino, Mario Tanomaru-Filho, Willian Fernando Zambuzzi, Flavio Henrique-Silva, Andrea Soares-Costa, Gisele Faria, Joni Augusto Cirelli
Cystatins are natural inhibitors of cysteine peptidases. Recently, cystatins derived from plants, named phytocystatins, have been extensively studied. Among them, CsinCPI-2 proteins from Citrus sinensis were identified and recombinantly produced by our group. Thus, this study described the recombinant expression, purification, and inhibitory activity of this new phytocystatin against human cathepsins K and B and assessed the anti-inflammatory effect of CsinCPI-2 in vitro in mouse and in vivo in rats. In addition, the pro-osteogenic effect of CsinCPI-2 was investigated in vitro...
June 18, 2019: Cytokine
Lan Hai, Maria M Szwarc, David M Lonard, Kimal Rajapakshe, Dimuthu Perera, Cristian Coarfa, Michael Ittmann, Rodrigo Fernandez-Valdivia, John P Lydon
Although salivary gland cancers comprise only ∼3-6% of head and neck cancers, treatment options for patients with advanced-stage disease are limited. Because of their rarity, salivary gland malignancies are understudied compared to other exocrine tissue cancers. The comparative lack of progress in this cancer field is particularly evident when it comes to our incomplete understanding of the key molecular signals that are causal for the development and/or progression of salivary gland cancers. Using a novel conditional transgenic mouse (K5:RANKL), we demonstrate that Receptor Activator of NFkB Ligand (RANKL) targeted to cytokeratin 5-positive basal epithelial cells of the salivary gland causes aggressive tumorigenesis within a short period of RANKL exposure...
June 18, 2019: Cytokine
Ying Dong, Kirsten Glaser, Nicolas Schlegel, Heike Claus, Christian P Speer
OBJECTIVES: Conventionally regarded as a harmless skin commensal, Staphylococcus epidermidis accounts for the majority of neonatal late-onset sepsis and is shown to be associated with neonatal inflammatory morbidities, especially bronchopulmonary dysplasia. This study addressed the pro-inflammatory capacity of different S. epidermidis strains on human alveolar epithelial cells. METHODS: A549 cell monolayers were stimulated by live bacteria of S. epidermidis RP62A strain (biofilm-positive) and ATCC 12228 strain (biofilm-negative) at a multiplicity of infection ratio of 10 for 24 h...
June 18, 2019: Cytokine
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