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DNA and Cell Biology

Ollin Celeste Martínez-Ramírez, Rebeca Pérez-Morales, Clementina Castro-Hernández, Maria Eugenia Gonsebatt, Leonora Casas-Ávila, Margarita Valdés-Flores, Pavel Petrosyan, Valeria Ponce de León-Suárez, Julieta Rubio
O6 -Methylguanine-DNA methyltransferase (MGMT) is an enzyme that repairs the DNA damage caused by the tobacco habit, and low activity of this enzyme has been associated with a risk of lung cancer (LC). Our objective was to determine the association of the promoter methylation and the rs12917 polymorphism of MGMT with formation of DNA bulky adducts and the risk of LC in the Mexican Mestizo population. In this study are included 431 subjects. High-resolution melting analysis was used to determine the polymorphism MGMT rs12917 and methylation levels...
February 20, 2019: DNA and Cell Biology
Mengyu Gao, Bingqi Zhang, Yuting He, Qing Yang, Lihong Deng, Yuqi Zhu, Enjiang Lai, Menghua Wang, Laduona Wang, Guang Yang, Guangneng Liao, Ji Bao, Hong Bu
The shortage of human hepatocytes continues to be a significant limitation for the widespread application of hepatocyte transplantation and bioartificial liver (BAL) support therapy. Recombinant activation gene 2 (Rag2) and fumarylacetoacetate hydrolase (Fah)-deficient mice could be highly repopulated with human hepatocytes. However, Fah/Rag2-deficient mice can only produce up to 1 × 108 human hepatocytes per mouse. We hypothesized that 2-10 × 1010 human hepatocytes can be produced per Fah/Rag2-deficient pig, which is an adequate supply for hepatocyte transplantation and BAL therapy...
February 14, 2019: DNA and Cell Biology
Sicheng Gao, Yun Miao, Yijie Liu, Xing Liu, Xingliang Fan, Yan Lin, Pingan Qian, Jun Zhou, Yaoyao Dai, Li Xia, Po Zhu, Junfeng Zhu
Abnormal expression of O-Linked β-N-acetylglucosamine (O-GlcNAc) and β-catenin is a general feature of cancer and contributes to transformed phenotypes. In this study, we identified the interaction between O-GlcNAc and β-catenin, and explored their effects on the progression of liver cancer. Our results demonstrated that upregulation of O-GlcNAc was induced by high glucose, whereas the application of PuGNAc and GlcNAc increased β-catenin protein expression levels, as well as the protein's stability and nuclear accumulation in the liver cancer cell lines HEP-G2 and HuH-7...
February 14, 2019: DNA and Cell Biology
Xiang Zhang, Bo Qiao, Zhenming Hu, Weidong Ni, Shuquan Guo, Gang Luo, Hanxiang Zhang, Honglei Ren, Lvetao Zou, Peng Wang, Wei Shui
Intervertebral disk degeneration (IDD) is a common disease that is caused by degeneration of the nucleus pulposus (NP). One goal in the treatment of IDD is delaying or reversing the degeneration of NP via the transformation of exogenous genes. This study first investigated the role of BMP9 in the extracellular matrix (ECM) of nucleus pulposus cells (NPCs) and its mechanism. We found that BMP9 promotes the expression of ECM in NPCs, and the key molecules of Notch signaling, namely, NICD-1, hes and hey, and it was significantly altered in BMP9-transfected NPCs, which suggests that BMP9 may regulate the ECM via the Notch signaling pathway...
February 13, 2019: DNA and Cell Biology
Ruoyi Qu, Xuelian Li, Xiaowei Quan, Lingzi Xia, Xue Fang, Hang Li, Baosen Zhou
CYP24A1 plays important roles in antiproliferative effects, which have been proved in many human tumor cells. Polymorphisms in CYP24A1 may affect the risk of lung cancer, but the results remained inconclusive. To enhance the understanding of possible relationship between CYP24A1 polymorphism rs6068816 and lung cancer risks, we first carried out this case-control study among Chinese female nonsmokers, including 345 lung cancer patients and 351 noncancer controls. Our results revealed that individuals carrying CT and CC genotype were associated with decreasing lung cancer risk (adjusted odds ratios were 0...
February 6, 2019: DNA and Cell Biology
Zilong Zhao, Fanjian Li, Qi Guo, Yuan Zhou, Yuyang Miao, Ying Li, Zengguang Wang, Rongcai Jiang, Jing-Fei Dong, Xiao Liu, Jianning Zhang, Yanjun Zhang
Collagen is a major component of the subendothelial matrix and participates in bleeding arrest by activating and aggregating platelets at the site of vascular injury. The most common type I collagen exists in both soluble and fibrillar forms, but structural exchangeability between the two forms is currently unknown. Using atomic force microscopy, we show that type I collagen switches between soluble and fibrillar forms in a pH-dependent and ion-independent manner. Fibrillar collagen is rope like with characteristic "D-bands...
February 6, 2019: DNA and Cell Biology
Xingxia Zhang, Weiguo Tao
The vital role of long noncoding RNAs (lncRNAs) on the acute myeloid leukemia (AML) has been increasingly recognized. This study aims to explore the unknown function of lncRNA LINC00152 in the leukemogenesis of AML. LINC00152 is determined to be upregulated in the AML samples, and the overexpression of LINC00152 is also authenticated in the advanced French-American-British (FAB) AML patients and closely correlated with the poor outcome of AML patients. The functional experiments state that knockdown of LINC00152 suppresses the proliferation, accelerates the apoptosis, and induces the cycle arrest of AML cells...
February 1, 2019: DNA and Cell Biology
Xiaohui Li, Ping Jiang, Haibin Yu, Yuwei Yang, Lixin Xia, Runjun Yang, Xibi Fang, Zhihui Zhao
MicroRNAs (miRNAs) can regulate a variety of biological functions such as fat and cholesterol synthesis, translocation, and utilization by regulating degradation or by inhibiting target mRNA translation. In this study, the target gene of miR-21-3p was analyzed through bioinformatics, which predicted Elovl5. Furthermore, miR-21-3p was verified for regulation of triglyceride expression. The results showed that luciferase activity was significantly lower in cells cotransfected with miR-21-3p and Elovl5 reporter vector, pmiR-RB-REPORT-Elovl5-WT, than in cells with miR-21-3p and reporter control vectors, pmiR-RB-REPORT-Elovl5-mut and pmiR-RB-REPORT-Elovl5-si, indicating that target sites exist in the 3'UTR of Elovl5...
February 1, 2019: DNA and Cell Biology
Yuanyuan Nie, Jiaxuan Li, Yan Jin, B L Grégoire Nyomba, Peter A Cattini, Hana Vakili
Pancreatic β-cell failure is characterized by compromised insulin secretion in response to glucose, which ultimately results in hyperglycemia, the clinical hallmark of type 2 diabetes mellitus (T2DM). Acute exposure to plasma free fatty acids (FFAs) potentiates glucose stimulated insulin secretion (GSIS), while chronic exposure impairs GSIS, and the latter has been associated with the mechanism of β cell failure in obesity linked T2DM. By contrast, growth hormone (GH) signaling has been linked positively to GSIS in β cells...
January 31, 2019: DNA and Cell Biology
Zohreh Rezaei, Ahmadreza Sebzari, Dor Mohammad Kordi-Tamandani, Kazem Dastjerdi
Resistance to trastuzumab has become a limiting factor for therapeutic efficacy of human epidermal growth factor 2 (HER2)-positive breast cancer. Different expression levels of miRNAs in cancer cells have been associated with poor prognosis and response to chemotherapy. The aim of this study was to evaluate miRNAs that were thought to be associated with HER2-positive breast cancer chemoresistance. In this study, the relative expression of candidate miRNAs to U6 RNA was evaluated in trastuzumab-resistant and trastuzumab-sensitive cells using relative real-time PCR...
January 31, 2019: DNA and Cell Biology
Yu Mao, Zhanzhao Fu, Lixin Dong, Yue Zheng, Jing Dong, Xin Li
As a common malignancy in women, cervical squamous cell carcinoma is a major cause of cancer-related mortality globally. Recent studies have demonstrated that long non-coding RNA (lncRNA) can function as potential biomarkers in cancer prognosis; however, little is known about its role in cervical cancer. In this study, we downloaded the gene expression profiles along with the clinical data of patients with cervical squamous cell carcinoma from The Cancer Genome Atlas. By applying bioinformatics analysis including random forest selection and Least Absolute Shrinkage and Selection Operator (LASSO) cox regression model along with 10-fold cross-validation, we constructed a 26-lncRNAs risk model that can be used to predict the overall survival of cervical squamous cell carcinoma...
January 30, 2019: DNA and Cell Biology
Shuangyin Zhang, Shixiong Gao, Yingbin Wang, Ping Jin, Fengjiao Lu
Long noncoding RNAs (lncRNAs) have been reported to play a key role in diversity cardiovascular diseases, including cardiac fibrosis. The present study aims to investigate the biological role of lncRNA SRA1 in the activation of cardiac myofibroblasts and the underlying mechanism. Results showed that the expression of SRA1 was upregulated accompanied by cardiac fibrosis in an abdominal aortic banding-treated rat model. Ang-II treatment increased the SRA1 expression of cardiac myofibroblasts, whereas SRA1 knockdown by siRNA inhibited the proliferation, myofibroblast conversion, and collagen production of cardiac myofibroblasts induced by Ang-II...
January 29, 2019: DNA and Cell Biology
Peng Wang, Lingling Ma, Shu'an Wang, Linfang Li, Qing Wang, Rutong Yang, Ya Li
The capacity to develop adventitious roots (AR) from cuttings is a key factor for the mass deployment of superior genotypes in the woody plant, including Catalpa Scop. Catalpa Scop. was characterized as having excellent woody qualities in China. However, the knowledge of the molecular mechanisms of AR formation is limited in Catalpa Scop. In this study, for the first time, association mapping for AR formation was performed on a selected sample of 108 Catalpa accessions. Genetic diversity and population structure was estimated on the basis of 54 gene-derived simple sequence repeat markers...
January 24, 2019: DNA and Cell Biology
Ce Yuan, Subbaya Subramanian
Worldwide, colorectal cancer (CRC) is one of the leading causes of cancer-related deaths. Recent advances in high-throughput technologies have shown that the gut microbiota may have a major influence on human health, including CRC. Nonetheless, how the gut microbiota interacts with tumor cells in CRC patients is largely unknown. Studies have shown that the microbiota fills in a variety of niche metabolic pathways that the host does not possess. For example, the microbiota produces butyrate, which provides the colon's epithelial cells with about 70% of their energy needs...
January 22, 2019: DNA and Cell Biology
Ming Su, Xinyi Hu, Jun Lin, Lei Zhang, Wen Sun, Jian Zhang, Ye Tian, Wei Qiu
Renal ischemia/reperfusion injury (IRI) is a main risk factor for the occurrence of delayed graft function or primary graft nonfunction of kidney transplantation. However, it lacks ideal molecular markers for indicating IRI in kidney transplantation. The present study is to explore novel candidate genes involved in renal IRI. Experimental renal IRI mouse models were constructed, and the differentially expressed genes were screened using a microarray assay. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis were performed...
January 22, 2019: DNA and Cell Biology
Yanyan Shi, Pan Wang, Yanlei Guo, Xiaoling Liang, Yuan Li, Shigang Ding
Helicobacter pylori is a major cause of gastric cancer. This study was aimed to explore the characteristic of DNA damage induced by H. pylori infection in gastric cancer AGS cells. After infection with H. pylori, the reactive oxygen species (ROS) levels in AGS cells were significantly higher than those in the uninfected cells. Cells with longer comet tails were detected after infection with H. pylori. The number of apurinic/apyrimidinic endonuclease 1- and phosphorylated H2AX-positive cells was significantly increased compared with the number of negative control cells...
January 18, 2019: DNA and Cell Biology
Gaspar Banfalvi
D-ribose and D-arabinose differ only by the steric orientation of their C2-OH groups. The initial reactions and emergence of RNA depended on the position, reactivity, and flexibility of the C2-OH moiety in the ribose molecule. The steric relationship of the C2- and C3-OH groups favored the selection of ribose, ribonucleotide, and RNA synthesis and excluded the possibility of xenonucleic acid-based life on Earth. This brief review provides a hypothesis based on the absence of nucleotides and enzymes under prebiotic conditions and on the polymerization of ribose 5-phosphate units leading to the polarized formation of the ribose-phosphate backbone...
January 14, 2019: DNA and Cell Biology
Xian-Min Shen, Jin Cheng
P-glycoprotein (P-gp), encoded by the MDR1 (multidrug resistance 1) gene, involves in the efflux of multiple compounds, such as certain antiepileptic drugs. The aim of this research was to observe the impacts of MDR1 (C3435T) variant on the efflux of phenytoin, carbamazepine, valproate, and phenobarbital in vitro. Stable recombinant LLC-PK1 cell systems transfected with MDR13435C (wild-type allele) and MDR13435T (variant allele) were constructed. The influences of MDR1 (C3435T) variant on the sensitivity, intracellular accumulation, and transepithelial permeability of antiepileptic drugs were assessed...
January 11, 2019: DNA and Cell Biology
Ya Zhang, Xin Zhang, Xiaowen Wang, Herui Wang, Xiaohui Wu, Hong Xu, Qian Shen
Congenital anomalies of the kidney and urinary tract (CAKUT) are the leading cause of end-stage renal disease in children. Our group has discovered that Holliday Junction resolvase gene Gen1 is a potential candidate gene for CAKUT. Gen1 mutant mice showed CAKUT phenotypes similar to those observed in retinoic acid (RA)-deficient models. The expression of Raldh2, which encodes the key enzyme in RA synthesis, was reduced in Gen1 mutant metanephros through RNA sequencing. By real-time reverse transcription-PCR, the expression of both Raldh2 and downstream Ret was reduced in embryonic day (E) 11...
January 11, 2019: DNA and Cell Biology
Linjie Wang, Xueying Li, Zhe Chao, Tao Zhong, Jiazhong Guo, Yan Wang, Li Li, Hongping Zhang
Nicotinamide phosphoribosyl transferase (NAMPT) is a cytokine which is secreted by adipose tissue and involved in the process of immunoreactions, inflammation, and lipid metabolism through the endocrine or paracrine. However, the information regarding NAMPT and its transcriptional regulation in goats remains unknown. In this study, the inactivation of glycogen synthase kinase 3β (GSK3β) decreased the supernatant NAMPT productions in goat adipocytes. The luciferase activities were kept in high levels in pGL3 (-735/+34) and the deletion from -735 bp to -486 bp significantly decreased the luciferase activity (p < 0...
January 7, 2019: DNA and Cell Biology
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