Yujin Xiao, Mengfan Zhou, Changgen Liu, Siyu Gao, Chao Wan, Shunji Li, Chenxi Dai, Wei Du, Xiaojun Feng, Yiwei Li, Peng Chen, Bi-Feng Liu
Public health events caused by pathogens have imposed significant economic and societal burdens. However, conventional methods still face challenges including complex operations, the need for trained operators, and sophisticated instruments. Here, we proposed a fully integrated and automated centrifugal microfluidic chip, also termed IACMC, for point-of-care multiplexed molecular diagnostics by harnessing the advantages of active and passive valves. The IACMC incorporates multiple essential components including a pneumatic balance module for sequential release of multiple reagents, a pneumatic centrifugation-assisted module for on-demand solution release, an on-chip silicon membrane module for nucleic acid extraction, a Coriolis force-mediated fluid switching module, and an amplification module...
March 28, 2024: Biosensors & Bioelectronics