Jinting Meng, Zihao Xu, Xinhao Li, Baozheng Wang, Xiaowei Zhang, Zikang Xie, Chen Zhang, Hong Wang, Yingwei Zhang
To achieve highly sensitive and reliable detection of apurinic/apyrimidinic endonuclease 1 (APE1), a critical cancer diagnostic biomarker, we designed a DNA walker-based dual-mode biosensor, utilizing cellular endogenous dual enzymes (APE 1 and Flap endonuclease 1 (FEN 1)) to collaborate in activating and propelling DNA walker motion on DNA-functionalized Au nanoparticles. Incorporating both fluorescence and electrochemical detection modes, this system leverages signal amplification from DNA walker movement and cascade amplification through tandem hybridization chain reactions (HCR), achieving highly sensitive detection of APE 1...
July 14, 2024: Biosensors & Bioelectronics
Yi He, Lijun Luo, Libo Li, Tianyan You, Xuegeng Chen
Molecularly imprinted polymers (MIP) have been widely owing to their specificity, however, their singular structure imposes limitations on their performance. Current enhancement methods, such as doping with inorganic nanomaterials or introducing various functional monomers, are limited and single, indicating that MIP performances require further advancement. In this work, a dual-modification approach that integrates both conductive inorganic nanomaterials and diverse bifunctional monomers was proposed to develop a multifunctional MIP-based electrochemical (MMIP-EC) sensor for diuron (DU) detection...
July 14, 2024: Biosensors & Bioelectronics
Hui Xu, Chenru Li, Renjie Mao, Xue Wang, Yufei Fan, Haijie Lu, Jing Liu, Hong Zhou
The development of dual-mode strategies with superior sensitivity and accuracy have garnered increasing attention for researchers in Aflatoxin B1 (AFB1) analysis. Herein, a colorimetric-electrochemiluminescence (ECL) dual-mode biosensor was constructed for onsite and ultrasensitive determination of AFB1. The multi-wall carbon nanotubes (MWCNTs) were integrated with the ZnO metal organic frameworks (MOFs) to accelerate the electron transfer and boost the ECL intensity of g-C3 N4 nanoemitters. Through the aptamer-based DNA sandwich assay, the CuO@CuPt nanocomposites were introduced onto the electrode and acted as the dual functional signal nanoprobes...
July 14, 2024: Biosensors & Bioelectronics
Shuzhen Lv, Yuting Zhou, Huijie Wang, Lingyi Kong, Sai Bi
A spatial-resolved and self-calibrated photoelectrochemical (PEC) biosensor has been fabricated by a multifunctional CeO2 /CdS heterostructure, achieving portable and sensitive detection of carcinoembryonic antigen (CEA) using a homemade 3D printing device. The CeO2 /CdS heterostructure with matched band structure is prepared to construct the dual-photoelectrodes to improve the PEC response of CeO2 . In particular, as the photoactive nanomaterial, the CeO2 also plays the role of peroxidase mimetic nanozymes...
July 14, 2024: Biosensors & Bioelectronics
Haixia Zhang, Yating Yang, Feiran Xie, Yu Lan, Lin Li, Zhenlei Song, Lu Gao, Yi Huang, Peihong Xiao
Drug-induced liver injury (DILI) poses a severe threat to public health. Endoplasmic reticulum (ER) stress contributes significantly to DILI pathogenesis, with peroxynitrite (ONOO- ) identified as a pivotal indicator. However, the temporal and spatial fluctuations of ONOO- associated with ER stress in the pathogenesis of DILI remain unclear. Herein, a novel ER-specific near-infrared (NIR) probe (QM-ONOO) with aggregation-induced emission (AIE) features for monitoring ONOO- fluctuations in DILI was elaborately constructed...
July 14, 2024: Biosensors & Bioelectronics
Yudie Yang, Xiankang Niu, Binqiu Duan, Jitao Lu, Xiaomei Zhang
In this study, a dual-mode biosensor based on the heterojunction of Cu2 O@Cu2 S/D-TA COF was constructed for ultra-sensitive detection of Hg2+ using both photoelectrochemical and electrochemical approaches. Briefly, a 2D ultra-thin covalent organic framework film (D-TA COF film) with excellent photoelectrochemical signals was prepared on ITO surfaces through an in situ growth method. Subsequently, the probe H1 was immobilized onto the biosensor via Au-S bonds. In the presence of Hg2+ , the formation of T-Hg2+ -T complexes triggered hybridization chain reactions (HCR), leading to the attachment of abundant Cu2 O@Cu2 S probes onto the biosensor...
July 13, 2024: Biosensors & Bioelectronics
Meritxell Rovira, Céline Lafaye, Silvia Demuru, Brince Paul Kunnel, Joan Aymerich, Javier Cuenca, Francesc Serra-Graells, Josep Maria Margarit-Taulé, Rubaiyet Haque, Mathieu Saubade, César Fernández-Sánchez, Cecilia Jimenez-Jorquera
The development of wearable devices for sweat analysis has experienced significant growth in the last two decades, being the main focus the monitoring of athletes health during workouts. One of the main challenges of these approaches has been to attain the continuous monitoring of sweat for time periods over 1 h. This is the main challenge addressed in this work by designing an analytical platform that combines the high performance of potentiometric sensors and a fluidic structure made of a plastic fabric into a multiplexed wearable device...
July 11, 2024: Biosensors & Bioelectronics
Yunqi Chen, Chen Xu, Mingyang Sun, Guanghua Zhao, Zhongjiang Wang, Chenyan Lv
Food safety and human health remain significant concerns in the food industry. Detecting food contaminants and diagnosing diseases are critical aspects. Ferritin, an iron storage protein widely found in nature, offers unique advantages. Its hollow protein nanocage structure, distinct interfaces, hydrophobic or hydrophilic channels, and B-C loop regions recognized by transferrin receptor 1 make ferritin versatile for detecting heavy metals, free radicals, and bioimaging both in vitro and in vivo. This review summarizes ferritin's general characteristics, its specific properties as biosensors, and its applications in food safety and in vivo imaging...
July 11, 2024: Biosensors & Bioelectronics
Heru Agung Saputra, Jae Heun Chung, Md Ali Zaber Sahin, Deog-Su Park, Yoon-Bo Shim
A disposable dual-output biosensor to detect program death-ligand 1 (PD-L1) was developed for immunotherapy progress monitoring and early cancer detection in a single experimental setup. The aptamer probe was assembled on rGO composited with carboxylated terthiophene polymer (rGO-pTBA) to specifically capture PD-L1 protein labeled with a new redox mediator, ortho-amino phenol para sulphonic acid, for amperometric detection. Each sensing layer was characterized through electrochemical and surface analysis experiments, then confirmed the sensing performance...
July 11, 2024: Biosensors & Bioelectronics
Jiawang Hu, Nanlin Hu, Donglei Pan, Yan Zhu, Xuan Jin, Shikai Wu, Yuan Lu
Non-invasive detection of tumors is of utmost importance to save lives. Nonetheless, identifying tumors through gas analysis is a challenging task. In this work, biosensors with remarkable gas-sensing characteristics were developed using a self-assembly method consisting of peptides and MXene. Based on these biosensors, a mimetic biosensor array (MBA) was fabricated and integrated into a real-time testing platform (RTP). In addition, machine learning (ML) algorithms were introduced to improve the RTP's detection and identification capabilities of exhaled gas signals...
July 10, 2024: Biosensors & Bioelectronics
Zhenhua Chen, Yang Xie, Yue Cao, Yu Wang, Meng Zhao, Yingsong Wu, Banglao Xu, Guanfeng Lin
Early and rapid diagnostic of acute myocardial infarction (AMI) during its developing stage is crucial due to its high fatality rate. Heart-type fatty acid binding protein (h-FABP) is an ideal biomarker for the quantitative diagnosis of AMI, surpassing traditional markers such as myoglobin, creatine phosphokinase-MB, and troponin in terms of sensitivity, specificity, and prognostic value. To obtain diagnostic and prognostic information, a precise and fully quantitative measurement of h-FABP is essential, typically achieved through an immunosorbent assay like the enzyme-linked immunosorbent assay...
July 10, 2024: Biosensors & Bioelectronics
Yuanyuan Cheng, Xuechi Yin, Yuechun Li, Shaochi Wang, Shouyu Xue, Qiaoying Wu, Jianlong Wang, Daohong Zhang
The multiple-readout capability of multimodal detection enhances the flexibility, reliability, and accuracy of lateral flow immunoassay (LFIA). The conjugation of two different metal-organic frameworks (MOFs) as a new-generation composite material offers extraordinary opportunities for developing multimodal LFIA. It is anticipated to compensate limitations of traditional single colorimetric signal LFIA and improve the analysis performance. Herein, an ultra-bright fluorescent AIE-MOF was proposed and coupled with an in-situ growth of Prussian blue (PB) nanoparticles strategy to obtain a novel multimodal signal tracer (AIE-MOF@PB)...
July 9, 2024: Biosensors & Bioelectronics
Yongzhuang Lu, Haoran Wang, Qiyao Li, Xiaoxu Zhang, Yuying Jia, Zheng Zhao, Yanfu Huan, Ben Zhong Tang
Researchers unremittingly strive to develop innovative luminophores to enhance intrinsic electrochemiluminescence (ECL) performance. However, the potential to harness facile strategies, such as manipulating the physical properties of luminophores while retaining functional chemical properties to fabricate cost-effective ECL complexes, remains underexplored. Herein, we reported a novel and efficient one-step galvanic technique to actualize aggregation-enhanced ECL (AEECL) of ruthenium complexes. It marked the first instance of the galvanic process being employed to synthesize aggregate luminophores through electrostatic attraction...
July 6, 2024: Biosensors & Bioelectronics
Sang-Mook You, Ki-Baek Jeong, Dong-Gook Kang, Sang-Min Kim, Young-Rok Kim
Glutathione (GSH) is indispensable for maintaining redox homeostasis in biological fluids and serves as a key component in cellular defense mechanisms. Accurate assessment of GSH relative to its oxidized counterpart, glutathione disulfide (GSSG), is critical for the early diagnosis and understanding of conditions related to oxidative stress. Despite existing methods for their quantification, the label-free and simultaneous measurement of GSH and GSSG in biological fluid presents significant challenges. Herein, we report the use of an alpha-hederin (Ah) nanopore for the direct measurement of the GSH:GSSG ratio in simulated biological fluid, containing fetal bovine serum (FBS)...
July 6, 2024: Biosensors & Bioelectronics
Lingjun Sha, Ya Cao, Lin Wang, Yujia Qin, Sha Zhu, Jing Zhao, Genxi Li
Circulating tumor cell (CTC) has been a valuable biomarker for the diagnosis of breast cancer, while folate receptor is a kind of cell surface receptor glycoprotein which is overexpressed in breast cancer. In this work, we have designed and fabricated an electrochemical biosensor for sensitive detection of folate receptor-positive CTCs based on mild reduction assisted CRISPR/Cas system. Specifically, folate functionalized magnetic beads are firstly prepared to capture CTCs owing to the strong affinity between folate and the folate receptors on the surface of cells...
July 6, 2024: Biosensors & Bioelectronics
Giulio Rosati, Arben Merkoçi, Ruslan Alvarez-Diduk, Anja Boisen, Alan Thomson
No abstract text is available yet for this article.
July 5, 2024: Biosensors & Bioelectronics
Hua Chai, Jiayue Shi, Yuan Zhuang, Peng Miao
Controllable assembly of DNA nanostructure provides a powerful way for quantitative analysis of various targets including nucleic acid molecules. In this study, we have designed detachable DNA nanostructures at electrochemical sensing interface and constructed a ligation chain reaction (LCR) strategy for amplified detection of miRNA. A three-dimensional DNA triangular prism nanostructure is fabricated to provide suitable molecule recognition environment, which can be further regenerated for additional tests via convenient pH adjustment...
July 4, 2024: Biosensors & Bioelectronics
Xuelian Lyu, Jiaru Fang, Dong Liu, Qianni Wu, Ying Li, Chunlian Qin, Jilin Zheng, Ning Hu
Bradyarrhythmia, a life-threatening cardiovascular disease, is an increasing burden for the healthcare system. Currently, surgery, implanted device, and drug are introduced to treat the bradyarrhythmia in clinical practice. However, these conventional therapeutic strategies suffer from the invasive surgery, power supply, or drug side effect, respectively, hence developing the alternative therapeutic strategy is necessarily imperative. Here, a convenient and effective strategy to treat the bradyarrhythmia is proposed using near-infrared-triggered Au nanorod (NR) based plasmonic photothermal effect (PPE)...
July 4, 2024: Biosensors & Bioelectronics
Yanis Berkane, Juan Pedro Cascales, Emmanuel Roussakis, Alexandre G Lellouch, Julian Slade, Nicolas Bertheuil, Mark A Randolph, Curtis L Cetrulo, Conor L Evans, Korkut Uygun
Continuous oxygenation monitoring of machine-perfused organs or transposed autologous tissue is not currently implemented in clinical practice. Oxygenation is a critical parameter that could be used to verify tissue viability and guide corrective interventions, such as perfusion machine parameters or surgical revision. This work presents an innovative technology based on oxygen-sensitive, phosphorescent metalloporphyrin allowing continuous and non-invasive oxygen monitoring of ex-vivo perfused vascularized fasciocutaneous flaps...
July 3, 2024: Biosensors & Bioelectronics
Hongwenjie Ma, Yicheng Tian, Derong Kong, Mingquan Guo, Changhao Dai, Qiang Wang, Shenwei Li, Zhengan Tian, Yunqi Liu, Dacheng Wei
An effective strategy for accurately detecting single nucleotide variants (SNVs) is of great significance for genetic research and diagnostics. However, strict amplification conditions, complex experimental instruments, and specialized personnel are required to obtain a satisfactory tradeoff between sensitivity and selectivity for SNV discrimination. In this study, we present a CRISPR-based transistor biosensor for the rapid and highly selective detection of SNVs in viral RNA. By introducing a synthetic mismatch in the crRNA, the CRISPR-Cas13a protein can be engineered to capture the target SNV RNA directly on the surface of the graphene channel...
July 2, 2024: Biosensors & Bioelectronics
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