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Human Cell

Akiyoshi Nakayama, Hirotaka Matsuo, Akira Ohtahara, Kazuhide Ogino, Masayuki Hakoda, Toshihiro Hamada, Makoto Hosoyamada, Satoshi Yamaguchi, Ichiro Hisatome, Kimiyoshi Ichida, Nariyoshi Shinomiya
Renal hypouricemia (RHUC) is a disease caused by dysfunction of renal urate reabsorption transporters; however, diagnostic guidance and guidelines for RHUC have been lacking, partly due to the low evidence level of studies on RHUC. This review describes a world-first clinical practice guideline (CPG) and its first version in English for this condition. It was developed following the "MINDS Manual for Guideline Development" methodology, which prioritizes evidence-based medicine. It was published in Japanese in 2017 and later translated into English...
February 19, 2019: Human Cell
Rieko Oyama, Fusako Kito, Mami Takahashi, Marimu Sakumoto, Kumiko Shiozawa, Zhiwei Qiao, Rei Noguchi, Takashi Kubo, Shunichi Toki, Fumihiko Nakatani, Akihiko Yoshida, Akira Kawai, Tadashi Kondo
Dedifferentiated chondrosarcoma is an aggressive mesenchymal tumor of the bone, and novel therapies are needed to improve its clinical outcomes. Patient-derived cell lines are essential tools for elucidating disease mechanisms associated with poor prognosis and for developing therapies. However, few lines and xenografts have been previously reported in dedifferentiated chondrosarcoma. We established a novel patient-derived dedifferentiated chondrosarcoma cell line, NCC-dCS1-C1. Primary dedifferentiated chondrosarcoma tissues were obtained at the time of surgery and subjected to primary tissue culture...
February 8, 2019: Human Cell
Fusako Kito, Rieko Oyama, Marimu Sakumoto, Kumiko Shiozawa, Zhiwei Qiao, Shunichi Toki, Akihiko Yoshida, Akira Kawai, Tadashi Kondo
Myxofibrosarcoma (MFS) is an aggressive sarcoma that requires novel therapeutic approaches to improve its clinical outcome. Cell lines are a valuable tool for pre-clinical research; however, there is a lack of patient-derived cell lines of MFS available from public cell banks. This study aimed to develop a patient-derived cell line of MFS. A cell line designated NCC-MFS1-C1 was established from the primary tumor tissue of an 82-year-old male patient with MFS. The short tandem repeat pattern of NCC-MFS1-C1 cells was identical to that of the original tumor, but distinct from that of any other cell lines in public cell banks...
February 8, 2019: Human Cell
Yoshinori Takei
New neurons are continuously generated in the adult brain. This generation primarily occurs in the subventricular zone (SVZ) of the lateral ventricles and the subgranular zone (SGZ) of the hippocampal dentate gyrus. In the SGZ, neural stem cells (NSCs) give rise to glutamatergic granule cells that integrate into the hippocampal circuitry. Reduction of neurogenesis in the hippocampus impairs learning and memory, which suggests that this process is important for adult hippocampal function. Indeed, the neurogenesis is reduced in the progression of aging, which is thought to contribute to age-related cognitive impairment...
February 7, 2019: Human Cell
Chunmei Cui, Yuehua Li, Ying Liu
Diabetic retinopathy (DR) is one of the common microvascular complications of diabetes mellitus, which is the main cause of blindness in diabetic patients. Angiogenesis plays an important role in retinal detachment and retinal microvascular inflammation throughout the whole development of DR. This study aimed to investigate the regulatory effect and the potential mechanism of miR-377 on high glucose and hypoxia-induced angiogenesis and inflammation in human retinal endothelial cells, and found that the miR-377 level was significantly increased after high glucose and hypoxia-mimetic agent to simulate the DR milieu...
January 31, 2019: Human Cell
Peipei Wu, Yong Chen
Chemotherapy-induced neuropathic pain (CINP) is a common and debilitating side effect of cancer treatment. Evodiamine, a major effective compound isolated from Evodia rutaecarpa, has been associated with anti-inflammatory and anti-nociceptive effects, an important therapeutic strategy for the treatment of neuropathic pain. However, the effects of evodiamine on CINP remain unknown. Thus, this study aims to investigate the pharmacological potential of evodiamine in attenuating paclitaxel-induced peripheral neuropathy...
January 30, 2019: Human Cell
Jie Li, Zhao-Ping Chu, Hua Han, Yuan Zhang, Fei Tian, Jun-Qin Zhang, Xiang-Hua Huang
This study explores the role of miR-93-5p in high-risk HPV-positive (HR-HPV) cervical cancer by targeting of BTG3. Cervical tissues were collected from 332 patients with conditions of chronic cervicitis (n = 42), low-grade cervical intraepithelial neoplasia (CIN I, n = 51), CIN II (n = 49), CIN III (n = 43), cervical cancer (n = 90), and normal cervical tissues (n = 57). HR-HPV DNA was detected by Hybrid Capture 2, and the expressions of miR-93-5p and BTG3 were determined by qRT-PCR and Western blot...
January 28, 2019: Human Cell
Naruwa Tokunaga, Ryota Kishi, Tomoko Sasai, Mikako Saito
To clarify the potential role of gap junction in cell-cell contact response, the expression of connexin30.3 gene (Cx30.3), a specifically expressed isoform in undifferentiated state of mouse embryonic stem (ES) cell line EB3 was investigated under different cell-cell contact conditions. ES cells were cultured by hanging drop culture method to increase cell-cell contact frequency. As control, a single cell culture was conducted. After culture for 12 h, the Cx30.3 expression level in hanging drop culture reached 1...
January 23, 2019: Human Cell
Tomomi Yokoyama, Hiromi Yagi Mendoza, Tomoko Tanaka, Hisataka Ii, Riya Takano, Ken Yaegaki, Hiroshi Ishikawa
Liver transplantation is the most effective treatment for treating liver cirrhosis. However, a limited number of donors, graft rejection, and other complications can undermine transplant success. It is considered that cell transplantation is an alternative approach of liver transplantation. We previously developed a protocol for hepatic differentiation of cluster of differentiation 117+ stem cells isolated from human exfoliated deciduous tooth pulp (SHEDs) under hydrogen sulfide exposure. These cells showed excellent hepatic function...
January 12, 2019: Human Cell
Yukihiro Seiji, Takaaki Ito, Yasuko Nakamura, Yuko Nakaishi-Fukuchi, Akira Matsuo, Naruki Sato, Hiroyuki Nogawa
Embryonic lungs were obtained from embryonic day 13.5 ICR mice. The lung-tip epithelium isolated using dispase treatment was embedded in low-growth factor Matrigel, cultured in DMEM/F12 medium containing 0.1% bovine serum albumin, supplemented with insulin, transferrin, and selenium (ITS), with or without fibroblast growth factor 7 (FGF7), and were observed for 14 days. With the addition of FGF7, the tip epithelium grew to form a cyst by culture day 7. Then, tubular tufts-like alveolus appeared around the cyst surface...
January 11, 2019: Human Cell
Chuanmeng Pan, Adheesh Bhandari, Yehuan Liu, Erjie Xia, Lizhi Lin, Shixu Lv, Ouchen Wang
Luminal B HER-2-negative (LBHN) subtype is one of the major subtypes of breast cancer according to different features, clinical behaviors, and treatment response. The LBHN subtype shows a poor prognosis and is insensitive to endocrine therapy. Our work aim is to investigate the prognostic factor in the LBHN subgroup and, meanwhile, try to obtain an optimal prognostic index (PI) contrapose LBHN subgroup which helps to guide chemotherapy. A total of 515 female LBNH patients who underwent diagnosis and surgery at our hospitals from August 2008 to August 2018 were enrolled...
December 17, 2018: Human Cell
Hajime Nomura, Kunihiko Umekita, Yuuki Hashikura, Kazumi Umeki, Ikuo Yamamoto, Yatsuki Aratake, Mineki Saito, Hiroo Hasegawa, Katsunori Yanagihara, Akihiko Okayama
We previously reported the diversity of structure and integration sites of human T-cell leukemia virus type 1 (HTLV-1) provirus among different MT-2 cell lines. This raised the question as to whether cell phenotypes also differed among MT-2 cell lines. The influence of two different MT-2 cell lines (MT-2J and MT-2B) on the growth of the promonocytic leukemia cell line, U937, was investigated. Protein levels and mRNA expression of cytokines were also investigated. In addition, Western blot analysis of HTLV-1 regulatory proteins, Tax and HBZ, was also performed...
December 17, 2018: Human Cell
Yanfeng Lu, Min Bu, Huifang Yun
Cardiomyocyte apoptosis plays an important role in ischemia/reperfusion (I/R)-induced myocardial injury. Autophagy is suggested to be widely implicated in regulating cell survival and death. The cardioprotection of sevoflurane postconditioning has been long recognized, but the underlying mechanisms are not well understood. This study aims to investigate whether the cardioprotective effects of sevoflurane are associated with autophagy regulation. An in vitro hypoxia/reoxygenation (H/R) model was established in human induced pluripotent stem cell-derived cardiomyocytes...
December 12, 2018: Human Cell
Yanming Lu, Yaqin Li, Wenjing Zhou, Bo Ding, Qing Yu
Natural killer T cells (NKT cells) and regulatory T cells (Treg cells) are two important immune regulatory cells which both play critical roles in asthma. Our previous experiments revealed that activation of Treg cells suppressed NKT cells in asthma. However, the possible regulatory effects and the mechanisms linking Treg cells and NKT cells remain poorly understood. The current study was designed to further investigate the regulatory effect and its possible mechanisms of Treg cells on NKT cells function, especially the distribution of NKT cells...
December 11, 2018: Human Cell
Miho Watanabe, Akihiro Ohyama, Hiroshi Ishikawa, Akira Tanaka
The regeneration of bone tissue is an active area of research, and future clinical applications are expected. Here, to establish new bone graft materials and an experimental bone tissue model, we generated united compact and spongy bone tissues containing vascular networks from human dental pulp stem cells in vitro. We applied the cell bead and cell sheet methods to construct three-dimensional bone tissue, which was cultured using a circumfusion apparatus for 30 days. Using micro-computed tomography, we assessed structural differences between compact and spongy bone...
December 6, 2018: Human Cell
Marie Fukutake, Daigo Ochiai, Hirotaka Masuda, Yushi Abe, Yu Sato, Toshimitsu Otani, Shigeki Sakai, Noriko Aramaki-Hattori, Masayuki Shimoda, Tadashi Matsumoto, Kei Miyakoshi, Yae Kanai, Kazuo Kishi, Mamoru Tanaka
Adult wound healing can result in fibrotic scarring (FS) characterized by excess expression of myofibroblasts and increased type I/type III collagen expression. In contrast, fetal wound healing results in complete regeneration without FS, and the mechanism remains unclear. Amniotic fluid cells could contribute to scar-free wound healing, but the effects of human amniotic fluid cells are not well characterized. Here, we determined the effect of human amniotic fluid stem cells (hAFS) on FS during wound healing...
December 1, 2018: Human Cell
Shanshan Lin, Bo Zhu, Guozhi Huang, Qing Zeng, Chuhuai Wang
Studies have demonstrated that mesenchymal stem cells (MSCs) can promote tumor growth, and MSC microvesicles (MVs) are very important in the tumor microenvironment and information transfer between cells during tumorigenesis and development. However, the potential effects and mechanisms of MSC-MVs on tumor growth are still controversial. Here in this study, we investigated the roles and effects of human bone marrow MSC-MVs (hBMSC-MVs) on human osteosarcoma (U2OS) cell growth under hypoxia in vitro and in vivo...
November 30, 2018: Human Cell
Chao Yang, Longshuang He, Gaofei Chen, Zeqian Ning, Zhibai Xia
Chloride (Cl- ), a primary anion in the extracellular fluid, plays an important role in a variety of physiological and pathological processes, such as cell apoptosis and proliferation. However, the information about Cl- in cancer cell apoptosis and chemoresistance is poorly understood. In the present study, we found that temozolomide (TMZ) treatment led to a decrease in intracellular concentration of Cl- ([Cl- ]i ) in both U87 and TMZ-resistant U87/R glioma cells. The decrease in [Cl- ]i was more noticeable in U87 cells than in U87/R cells...
November 13, 2018: Human Cell
Huanhuan Dong, Shuping Ming, Jie Fang, Yun Li, Ling Liu
Cerebrovascular smooth muscle cells (SMCs) hyperplasia is an important contributor to cerebrovascular remodeling during hypertension. The aim of present study was to investigate the effects of Icariin on cerebrovascular SMCs proliferation and remodeling and the underlying mechanisms. The results revealed that Icariin administration attenuated the enhanced basilar artery constriction in angiotensin II (AngII)-induced hypertension rat model, as well as the inhibition of basilar artery diameter reduction in response to AngII and phenylephrine...
November 1, 2018: Human Cell
Yuta Kinoshita, Seiya Arita, Haruka Murazoe, Kazuo Kitamura, Shinya Ashizuka, Kyoko Inagaki-Ohara
Adrenomedullin (AM) exerts a potent anti-inflammatory effect. Intrarectal or consecutive intravenous administrations of AM reduce pathological manifestations in rodent colitis models. However, in clinical applications, a safer administration route that provides stronger alleviation of patient burden is preferred. We investigated whether subcutaneously administered AM is effective against dextran sulfate sodium (DSS)-induced colitis. C57BL/6J mice were administered 1% DSS in drinking water and received AM at 8, 40 or 80 nmol/kg subcutaneously once a day for 7 consecutive days...
October 10, 2018: Human Cell
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