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Research in Microbiology

Can Wang, Puyuan Li, Wenkai Niu, Xin Yuan, Huiying Liu, Yong Huang, Xiaoping An, Hang Fan, Lilan Zhangxiang, Liyuan Mi, Jing Zheng, Yannan Liu, Yigang Tong, Zhiqiang Mi, Changqing Bai
Klebsiella pneumoniae is one of the major Gram-negative bacterial pathogens causing hospital-acquired multidrug-resistant infections, and the antimicrobial treatment options are scarce. The lack of available antimicrobials has prompted the development of alternative strategies for the treatment of these infections. In this study, a K. pneumoniae bacteriophage (vB_KpnP_IME321) targeting a KN1 capsular type strain, Kp409, was isolated, characterized and sequenced. This bacteriophage has a latent period of 20 min and a burst size of approximately 410 pfu/cell...
February 1, 2019: Research in Microbiology
Hong-Yeoul Ryu, Ruxin Duan, Seong Hoon Ahn
Protein arginine methylation, an evolutionarily conserved post-translational modification, serves critical cellular functions by transferring a methyl group to a variety of substrates, including histones and some transcription factors. In budding yeast, Hsl7 (histone synthetic lethal 7) displays type II PRMT (protein arginine methyltransferase) activity by generating symmetric dimethylarginine residues on histone H2A in vitro. However, identification of the in vivo substrate of Hsl7 and how it contributes to important cellular processes remain largely unexplored...
January 17, 2019: Research in Microbiology
Lingyan Jiang, Xiaomin Li, Runxia Lv, Lu Feng
Salmonella enterica serovar Typhimurium uses virulence factors encoded by Salmonella pathogenicity island 1 (SPI-1) to invade the intestinal epithelium. The low oxygen-induced transcriptional regulator LoiA is established to be a positive regulator of SPI-1 genes and can also repress the expression of the ATP-dependent Lon protease, a negative regulator of SPI-1 genes. Whether the repression of lon by LoiA is associated with its regulation of SPI-1 genes, as well as the regulatory mechanism involved, is unknown...
January 12, 2019: Research in Microbiology
Xia Yan, Jun Tao, Hong-Li Luo, Lei-Tao Tan, Wei Rong, Hui-Ping Li, Chao-Zu He
Xanthomonas campestris pv. campestris (Xcc) secretes a suite of effectors into host plants via the type III secretion system (T3SS), modulating plant immunity defenses. Strain Xcc8004 causes black rot in brassica plants, including Arabidopsis thaliana, making it a classical model for the study of Xanthomonas pathogenesis. XopLXcc8004 was defined as a T3SS effctor (T3SE) since its homologues XopLXcv85-10 from X campestris pv. vesicatoria (Xcv85-10) contribute to virulence in host plants. Except for its virulence on Chinese radish plants, little was previously known about the regulation and function of XopLXcc8004 ...
December 27, 2018: Research in Microbiology
Agnieszka Torzewska, Katarzyna Bednarska, Antoni Różalski
Infectious urolithiasis is a consequence of long-standing urinary tract infections with urease-positive bacteria, especially Proteus spp. However, because of the often mixed nature of urinary tract infections, in the case of urinary stones formation, several species of bacteria may be involved in the process. The purpose of the study was to determine the impact of the bacterial species: Escherichia coli, Klebsiella pneumoniae, Providencia stuartii, Pseudomonas aeruginosa and Staphylococcus aureus on the crystallization caused by Proteus mirabilis...
December 3, 2018: Research in Microbiology
Lenka Fišarová, Roman Pantůček, Tibor Botka, Jiří Doškař
Coagulase-negative staphylococci (CoNS) are an important cause of human and animal diseases. Treatment of these diseases is complicated by their common antimicrobial resistance, caused by overuse of antibiotics in hospital and veterinary environment. Therefore, they are assumed to serve as a reservoir of resistance genes often located on plasmids. In this study, we analyzed plasmid content in 62 strains belonging to 10 CoNS species of human and veterinary origin. In 48 (77%) strains analyzed, 107 different plasmids were detected, and only some of them showed similarities with plasmids found previously...
November 29, 2018: Research in Microbiology
Williamson Gustave, Zhao-Feng Yuan, Raju Sekar, Veronica Toppin, Jinjing-Yuan Liu, Yu-Xiang Ren, Jun Zhang, Zheng Chen
Soil Microbial Fuel Cells (MFCs) are devices that can generate electricity by using the flooded soil's anode respiring microbial consortium. When the MFC starts to work, the microbial community in the anode vicinity rapidly changes. This shift in the microbial community results in many dead cells that may release their DNA (relic DNA) and obscure culture independent estimates of microbial community composition. Although relic DNA is expected to increase in MFCs, the effect of relic DNA has not been investigated in the soil MFCs system...
November 20, 2018: Research in Microbiology
Ramkumar Iyer, Samir H Moussa, Ruben Tommasi, Alita A Miller
The major Gram-negative gated efflux channel TolC has been extensively characterized in Escherichia coli but there is minimal information about Klebsiella pneumoniae TolC. Using an arabinose-inducible plasmid-based expression system, we show that the K. pneumoniae TolC complements the efflux defect in an E. coli K-12 ΔtolC strain, restoring wild-type levels of resistance towards most antibiotics suggesting that it can interact with the E. coli AcrB efflux pump. We characterize the efflux properties of K. pneumoniae TolC using an orthogonal whole cell-based assay and quantify the extrusion of environment-sensitive fluorescent probes and contrast the findings with the E...
November 20, 2018: Research in Microbiology
Ruijuan Li, Linda Helbig, Jun Fu, Xiaoying Bian, Jennifer Herrmann, Michael Baumann, A Francis Stewart, Rolf Müller, Aiying Li, Daniel Zips, Youming Zhang
Abnormal blood vessels and hypoxic and necrotic regions are common features of solid tumors and related to the malignant phenotype and therapy resistance. Certain obligate or facultative anaerobic bacteria exhibit inherent ability to colonize and proliferate within solid tumors in vivo. Escherichia coli Nissle 1917 (EcN), a non-pathogenic probiotic in European markets, has been known to proliferate selectively in the interface between the viable and necrotic regions of solid tumors. The objective of this study was to establish a tumor-targeting therapy system using the genetically engineered EcN for targeted delivery of cytotoxic compounds, including colibactin, glidobactin and luminmide...
November 14, 2018: Research in Microbiology
Hebin Liao, Xiaowu Zhong, Lei Xu, Qiang Ma, Yongjian Wang, Yan Cai, Xiaolan Guo
OxyR is an important regulatory protein that plays a key role in anti-oxygenation, and its deletion causes a special VBNC (Viable But Non-Culturable) state in many bacteria including Salmonella typhimurium. The S. typhimurium in the VBNC state can grow in LB broth but cannot grow on an LB plate unless its concentration is sufficiently high. However, the mechanism that reverses this state is not clear. In this study, conditioned media containing autoinducer collected from the wild type strain can restore the growth of low concentrations of the oxyR mutant strain on LB plates, and S...
November 7, 2018: Research in Microbiology
Tantan Gao, Mingzheng Ding, Ching-Hong Yang, Haiyan Fan, Yunrong Chai, Yan Li
The rhizosphere bacterium Bacillus cereus 905 is capable of promoting plant growth through effective colonization on plant roots. The sodA2-encoding manganese-containing superoxide dismutase (MnSOD2) is important for survival of B. cereus 905 in the wheat rhizosphere. However, the genes involved in regulating sodA2 expression and the mechanisms of rhizosphere colonization of B. cereus 905 are not well elucidated. In this study, we found that the deletion of the ptsH gene, which encodes the histidine-phosphorylatable protein (HPr), a component of the phosphotransferase system (PTS), causes a decrease of about 60% in the MnSOD2 expression...
November 3, 2018: Research in Microbiology
Catherine J Edward, Athanasios Kotsiopoulos, Susan T L Harrison
Leptospirillum ferriphilum is the dominant iron-oxidising bacterium in traditional microbial communities utilised in bioprocesses for gold recovery from sulfidic minerals. Ferrous iron oxidation activity and growth of unadapted and thiocyanate-adapted L. ferriphilum HT was studied in batch culture across increasing thiocyanate (SCN- ) concentrations in the range 0-2 mg/L to assess the feasibility of recycling remediated cyanidation wastewaters. Thiocyanate concentrations of 1 mg/L and 1.4 mg/L induced an inhibitory effect in the unadapted culture wherein ferrous iron oxidation rate and cell growth were compromised...
November 2, 2018: Research in Microbiology
Laurent Debarbieux, Patrick Forterre, Mart Krupovic, Mzia Kutateladze, David Prangishvili
No abstract text is available yet for this article.
November 2, 2018: Research in Microbiology
Tomas Hessler, Susan T L Harrison, Robert J Huddy
Biological sulphate reduction (BSR) is a promising low-cost treatment of acid rock drainage effluents. In this paper, the system performance and microbial ecology of a lactate supplemented BSR up-flow anaerobic packed bed reactor (UAPBR) are evaluated across reactor height and compared to a continuous stirred tank reactor (CSTR). The biomass concentrations of planktonic and biofilm communities were quantified and subsequently characterised by 16S rRNA gene amplicon sequencing. The defined microbial communities were shown to correlate with differing availability of lactate, volatile fatty acids produced from lactate degradation and sulphate concentration...
October 8, 2018: Research in Microbiology
Bo Zeng, Zhiwen Lai, Lijin Sun, Zhongbao Zhang, Jianhua Yang, Zaixin Li, Jie Lin, Zhi Zhang
Polycystic ovary syndrome (PCOS) is a complex endocrine and metabolic disorder that affects 9-21% of reproductive-aged women. Affected women frequently display obesity, insulin resistance, and inflammation. Altered gut microbial community has been reported in PCOS and obese PCOS patients. However, the profile of the gut microbial community in insulin resistant PCOS (IR-PCOS) patients still remains unknown. In this study, next-generation sequencing based on the 16S rRNA gene was used to compare the gut microbial composition of women with IR-PCOS (n = 9, PCOS with insulin resistance), NIR-PCOS (n = 8, PCOS alone) and healthy controls (n = 8, HC)...
October 4, 2018: Research in Microbiology
Shintaro Maeno, Akinobu Kajikawa, Leon Dicks, Akihito Endo
Fructophilic lactic acid bacteria (FLAB) are unique in the sense that they prefer D-fructose over D-glucose as main carbon source. If D-glucose is metabolised, electron acceptors are required and significant levels of acetate are produced. These bacteria are found in environments rich in D-fructose, such as flowers, fruits and the gastrointestinal tract of insects feeding on fructose-rich diets. Fructobacillus spp. are representatives of this unique group, and their fructophilic characteristics are well conserved...
October 3, 2018: Research in Microbiology
Alexandro Guterres, Jonathan Gonçalves, Elba Regina Sampaio de Lemos
Bartonellosis is an emerging serious diseases of zoonotic relevance caused by an expanding number of recently discovered species of Bartonella. Many studies use partial gltA gene sequencing as the method of choice for species attribution, and a plethora of studies have utilized only this gene to describe Bartonella diversity. We observe a lack of correspondence between the phylogenies constructed using the complete gltA gene sequences and the small gene fragment usually used in phylogenetic analyzes. Everything indicates that important changes occurred by using larger fragments...
September 12, 2018: Research in Microbiology
Anke Licht, Martin Bommer, Tobias Werther, Kristin Neumann, Carolin Hobe, Erwin Schneider
Canonical ATP-binding cassette import systems rely on extracellular substrate binding proteins (SBP) for function. In gram-negative bacteria, SBPs are usually freely diffusible in the periplasm and, where studied, exist in excess over their cognate transporters. However, in vitro studies with the maltose transporter of Escherichia coli (MalFGK2 ) have demonstrated that mechanistically one copy of its SBP (MalE) per transport complex is sufficient for activity. To address whether such a condition is physiologically relevant, we have characterized a homolog of the E...
September 4, 2018: Research in Microbiology
Klaas Martinus Pos
No abstract text is available yet for this article.
September 2018: Research in Microbiology
Zhongxin Luo, Shengjie Li, Kun Hou, Guodong Ji
In this study, the spatial and seasonal bacterioplankton community dynamics were investigated in the main channel of the Middle Route of the South-to-North Water Diversion Project (MRP) using Illumina HiSeq sequencing. Water samples were collected in spring and summer from south to north at eight water quality monitoring stations, respectively. The results showed that seasonal changes had a more pronounced effect on the bacterioplankton community compositions (BCCs) than spatial variation. The diversity analysis results indicated that samples of summer have more operational taxonomic units (OTUs), higher richness and diversity than those in spring...
September 1, 2018: Research in Microbiology
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