Cameron Sadegh, Huixin Xu, Jason Sutin, Benoit Fatou, Suhasini Gupta, Aja Pragana, Milo Taylor, Peter N Kalugin, Miriam E Zawadzki, Osama Alturkistani, Frederick B Shipley, Neil Dani, Ryann M Fame, Zainab Wurie, Pratik Talati, Riana L Schleicher, Eric M Klein, Yong Zhang, Michael J Holtzman, Christopher I Moore, Pei-Yi Lin, Aman B Patel, Benjamin C Warf, W Taylor Kimberly, Hanno Steen, Mark L Andermann, Maria K Lehtinen
Post-hemorrhagic hydrocephalus (PHH) refers to a life-threatening accumulation of cerebrospinal fluid (CSF) that occurs following intraventricular hemorrhage (IVH). An incomplete understanding of this variably progressive condition has hampered the development of new therapies beyond serial neurosurgical interventions. Here, we show a key role for the bidirectional Na-K-Cl cotransporter, NKCC1, in the choroid plexus (ChP) to mitigate PHH. Mimicking IVH with intraventricular blood led to increased CSF [K+ ] and triggered cytosolic calcium activity in ChP epithelial cells, which was followed by NKCC1 activation...
March 1, 2023: Neuron