journal
https://read.qxmd.com/read/37841960/effective-methods-for-immobilization-of-non-adherent-pv11-cells-while-maintaining-their-desiccation-tolerance
#21
JOURNAL ARTICLE
Hiroto Fuse, Takahiro Kikawada, Richard Cornette
UNLABELLED: Pv11 was derived from embryos of the sleeping chironomid Polypedilum vanderplanki , which displays an extreme form of desiccation tolerance known as anhydrobiosis. Pre-treatment with a high concentration of trehalose allows Pv11 cells to enter anhydrobiosis. In the dry state, Pv11 cells preserve transgenic luciferase while retaining its activity. Thus, these cells could be utilized for dry-preserving antibodies, enzymes, signaling proteins or other valuable biological materials without denaturation...
December 2023: Cytotechnology
https://read.qxmd.com/read/37841959/deciphering-variations-in-the-endocytic-uptake-of-a-cell-penetrating-peptide-the-crucial-role-of-cell-culture-protocols
#22
JOURNAL ARTICLE
Joshua Diaz, Jean-Philippe Pellois
UNLABELLED: Delivery tools, including cell-penetrating peptides (CPPs), are often inefficient due to a combination of poor endocytosis and endosomal escape. Aspects that impact the delivery of CPPs are typically characterized using tissue culture models. One problem of using cell culture is that cell culture protocols have the potential to contribute to endosomal uptake and endosomal release of CPPs. Hence, a systematic study to identify which aspects of cell culturing techniques impact the endocytic uptake of a typical CPP, the TMR-TAT peptide (peptide sequence derived from HIV1-TAT with the N-terminus labeled with tetramethylrhodamine), was conducted...
December 2023: Cytotechnology
https://read.qxmd.com/read/37841958/hypoxia-induced-the-upregulation-of-ndufa4l2-promoted-colon-adenocarcinoma-progression-through-ros-mediated-pi3k-akt-pathway
#23
JOURNAL ARTICLE
Nianyuan Ye, Yibo Wang, Peng Jiang, Huaji Jiang, Wei Ding, Zheng Zhang, Cheng Xi
The NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 4-like 2 (NDUFA4L2) gene has been reported to be upregulated in colorectal cancer (CRC) and is associated with worse prognosis. However, the specific function and underlying mechanism of NDUFA4L2 in colon adenocarcinoma (COAD) under hypoxia has never been investigated. Our study discovered that hypoxia promoted the viability, metastasis, and epithelial-mesenchymal transition (EMT) of COAD cells. Besides, hypoxia-induced HIF-1α upregulated the expression of NDUFA4L2 which served as an oncogene and an independent diagnostic and prognostic marker in COAD...
December 2023: Cytotechnology
https://read.qxmd.com/read/37841957/mir-224-5p-inhibits-osteoblast-differentiation-and-impairs-bone-formation-by-targeting-runx2-and-sp7
#24
JOURNAL ARTICLE
Siyang Ding, Yunfei Ma, Jiashu Yang, Yuting Tang, Yucui Jin, Lingyun Li, Changyan Ma
UNLABELLED: Osteoporosis is a complicated multifactorial disorder characterized by low bone mass and deteriorated bone microarchitecture with an elevated fracture risk. MicroRNAs play important roles in osteoblastic differentiation. In the present study, we found that miR-224-5p was markedly downregulated during the osteogenic differentiation of C2C12 cells. Overexpression of miR-224-5p in C2C12 cells inhibited osteoblast activity, as indicated by reduced ALP activity, matrix mineralization and the expression of osteogenic marker genes...
December 2023: Cytotechnology
https://read.qxmd.com/read/37841956/the-m6a-reader-igf2bp1-manipulates-bub1b-expression-to-affect-malignant-behaviors-stem-cell-properties-and-immune-resistance-of-non-small-cell-lung-cancer-stem-cells
#25
JOURNAL ARTICLE
Shuo Hu, Xi Yan, Wen Bian, Bin Ni
N6-methyladenosine (m6A) modification is the most common internal modification in eukaryotic mRNA and an important mechanism for post-transcriptional regulation of genes. This study focuses on the role of the m6A reader insulin-like growth factor 2 mRNA binding protein 1 (IGF2BP1) in the malignant behaviors of non-small-cell lung cancer (NSCLC) cells and especially the cancer stem cells (CSCs). We obtained IGF2BP1 as an aberrantly upregulated gene linking to poor survival of patients with NSCLC by bioinformatics, and then confirmed increased IGF2BP1 expression in NSCLC tissues and cells, especially in the enriched CSCs...
December 2023: Cytotechnology
https://read.qxmd.com/read/37655276/assessing-mtt-and-sulforhodamine-b-cell-proliferation-assays-under-multiple-oxygen-environments
#26
JOURNAL ARTICLE
Ming Yao, Glenn Walker, Michael P Gamcsik
UNLABELLED: Cell proliferation can be measured directly by counting cells or indirectly using assays that quantitate total protein or metabolic activity. However, for comparing cell proliferation under varying oxygen conditions it is not clear that these assays are appropriate surrogates for cell counting as cell metabolism and protein synthesis may vary under different oxygen environments. We used permeable bottom tissue culture ware to compare proliferation assays as a function of static oxygen concentrations under oxygen partial pressure ( p O2 ) levels ranging from 2 to 139 mmHg...
October 2023: Cytotechnology
https://read.qxmd.com/read/37655275/h3k27-acetylation-induced-fstl1-upregulation-by-p300-runx1-co-activation-exacerbated-autophagy-mediated-neuronal-damage-and-nf-%C3%AE%C2%BAb-stimulated-inflammation-in-alzheimer-s-disease
#27
JOURNAL ARTICLE
Dongmei Dai, Junzheng Xie, Yun Zheng, Fangbin Chen, Bin Zhao, Li Miao
Follistatin-like protein 1 (FSTL1) has been demonstrated to participate in the pathogenesis of several neurological diseases. The current study informed the role of H3K27 acetylation-induced FSTL1 upregulation in Alzheimer's disease (AD). Our investigation discovered the upregulated FSTL1 expression and enhanced autophagy activity in AD. FSTL1 knockdown successfully attenuated the injuries of Aβ1-42 -challenged SH-SY5Y cells through the inhibition of autophagy activity. Besides, FSTL1 deficiency suppresses the inflammatory response and NF-κB signaling in AD...
October 2023: Cytotechnology
https://read.qxmd.com/read/37655274/effect-assessment-of-a-type-of-xeno-free-and-serum-free-human-adipose-derived-mesenchymal-stem-cells-culture-medium-by-proliferation-and-differentiation-capacities
#28
JOURNAL ARTICLE
Shanshan Chen, Li Meng, Shanshan Wang, Yan Xu, Wenbin Chen, Jianfeng Wei
Human mesenchymal stem cells (hMSCs) possess broad prospects in pre-clinical research. In vitro amplification of hMSCs requires appropriate medium to reach the number of seed cells with clinical significance. However, the uncertainty of the heterologous components of the traditional fetal bovine serum (FBS) culture medium has great safety risks. Moreover, existing commercial hMSCs medium is very expensive, therefore a safer and more optimal hMSCs medium is urgently needed. Accordingly, we developed five components adipose-derived hMSCs (hADMSCs) medium without xenogenic components, named E5 SFM...
October 2023: Cytotechnology
https://read.qxmd.com/read/37655273/identification-of-differentially-expressed-mirnas-and-mrnas-associated-with-the-regulation-of-breast-cancer-via-in-silico-and-in-vitro-methods
#29
JOURNAL ARTICLE
Pelin Telkoparan-Akillilar, Dilek Cevik
miRNA expressions are altered during development of breast cancer (BC). The aim of this study is to identify novel cancer-related miRNAs and pathways to understand the mechanisms of BC subtypes. GSE59247 dataset was downloaded from gene expression omnibus (GEO) database and analyzed with GEO2R software. The differential miRNA expressions in BC cells were evaluated by miRNome PCR array. Venn diagram was used to reveal co-differentially expressed miRNAs between GSE59247 dataset and miRNome array. Clinical prognostic significance of selected miRNAs was evaluated via Kaplan Meier curve...
October 2023: Cytotechnology
https://read.qxmd.com/read/37655272/effect-of-milk-and-whey-on-proliferation-and-differentiation-of-placental-stromal-cells
#30
JOURNAL ARTICLE
Bircan Boga, Merve Akbulut, Erkan Maytalman, Ilknur Kozanoglu
UNLABELLED: Fetal bovine serum (FBS), which is widely used in cell culture media, has the potential to cause medical and ethical problems. Here, an experimental study using milk or whey proteins containing essential nutrients and growth factors is presented to limit the use of FBS in cell culture media produced for cell and tissue regeneration. Study groups were formed by culturing human placenta mesenchymal stem cells, known to have high proliferation and differentiation capacity, with milk or whey solution at increasing concentrations, alone or in combination with FBS...
October 2023: Cytotechnology
https://read.qxmd.com/read/37655271/new-high-throughput-screening-method-for-chinese-hamster-ovary-cell-lines-expressing-low-reduced-monoclonal-antibody-levels-application-of-a-system-controlling-the-gas-phase-over-cell-lysates-in-miniature-bioreactors-and-facilitating-multiple-sample-setup
#31
JOURNAL ARTICLE
Tsuyoshi Yamaguchi, Mie Fukuda, Yuichi Matsumoto, Takaaki Mori, Shinsuke Kikuchi, Ryuma Nagano, Koichi Yamamoto, Kaori Wakamatsu
Interchain disulfide bonds in monoclonal antibodies may be reduced during large-scale mAb production using Chinese hamster ovary (CHO) cells. This reaction lowers the mAb product yield and purity; however, it may be prevented by screening cell lines that are unsusceptible to reduction and using them in mAb production. Antibody reduction susceptibility may be cell line-dependent. To the best of our knowledge, however, an efficient method of screening reduction-unsusceptible CHO cell lines has not been previously reported...
October 2023: Cytotechnology
https://read.qxmd.com/read/37655270/lobetyolin-inhibits-cell-proliferation-and-induces-cell-apoptosis-by-downregulating-asct2-in-gastric-cancer
#32
JOURNAL ARTICLE
Lin Cheng, Haoqing Zhai, Juan Du, Gang Zhang, Gan Shi
Gastric cancer (GC) is a heterogeneous disease and is the fifth most common cancer worldwide. Lobetyolin, as a bioactive ingredient extracted from Codonopsis pilosula (Franch.) Nannf. , has been reported to exert anti-tumor effects in several cancer types. This study was aimed to investigate the role of lobetyolin in GC and the associated mechanism. MKN-45 and MKN-28 cells were incubated with concentrations of lobetyolin for 24 h. The viability and survival of GC cells were evaluated by performing MTT assay...
October 2023: Cytotechnology
https://read.qxmd.com/read/37389132/fabricating-the-cartilage-recent-achievements
#33
REVIEW
Nesa Fani, Maria Peshkova, Polina Bikmulina, Reihaneh Golroo, Peter Timashev, Massoud Vosough
This review aims to describe the most recent achievements and provide an insight into cartilage engineering and strategies to restore the cartilage defects. Here, we discuss cell types, biomaterials, and biochemical factors applied to form cartilage tissue equivalents and update the status of fabrication techniques, which are used at all stages of engineering the cartilage. The actualized concept to improve the cartilage tissue restoration is based on applying personalized products fabricated using a full cycle platform: a bioprinter, a bioink consisted of ECM-embedded autologous cell aggregates, and a bioreactor...
August 2023: Cytotechnology
https://read.qxmd.com/read/37389131/an-agarose-alginate-microfluidic-device-for-the-study-of-spheroid-invasion-atra-inhibits-cafs-mediated-matrix-remodeling
#34
JOURNAL ARTICLE
Mohammad Reza Nasiraee, Shabnam Shahrivari, Soheila Sayad, Hoda Mahdavi, Neda Saraygord-Afshari, Zeinab Bagheri
UNLABELLED: Growing evidence demonstrates that cancer-associated fibroblasts (CAF) are responsible for tumor genesis, growth, metastasis, and treatment response. Therefore, targeting these cells may contribute to tumor control. It has been proposed that targeting key molecules and pathways of proliferative functions can be more effective than killing CAFs. In this regard, multicellular aggregates, like spheroids, can be used as human tumor models. Spheroids closely resemble human tumors and mimic many of their features...
August 2023: Cytotechnology
https://read.qxmd.com/read/37389130/development-of-a-cell-line-from-skeletal-trunk-muscle-of-the-fish-labeo-rohita
#35
JOURNAL ARTICLE
Mukunda Goswami, Nevil Pinto, B S Yashwanth, A Sathiyanarayanan, Reza Ovissipour
Labeo rohita is a widely cultivated tropical freshwater carp and found in rivers of South Asian region. A new cell line, designated LRM, has been developed from the muscle tissue of L. rohita . Muscle cells were subcultured up to 38 passages in a Leibovitz's-15 (L-15) supplemented with 10% FBS (Fetal Bovine Serum) and 10 ng/ml bFGF. The LRM cells exhibited fibroblastic morphology with a doubling time of 28 h, and a plating efficiency of 17%. A maximum growth rate was observed for LRM cells at 28 °C, 10% FBS and 10 ng/ml bFGF...
August 2023: Cytotechnology
https://read.qxmd.com/read/37389129/m2-macrophage-derived-extracellular-vesicles-augment-immune-evasion-and-development-of-colorectal-cancer-via-a-circrna_ccdc66-microrna-342-3p-metadherin-axis
#36
JOURNAL ARTICLE
Linfeng Fan, Guofeng Xu, Xiangfu Zeng
The M2 macrophages are major components in the tumor microenvironment and are closely linked to immune suppression and tumor metastasis. This work focuses on how M2 macrophage-derived extracellular vesicles (EVs) affect colorectal cancer (CRC) progression. THP-1 monocytes were induced to differentiate to M0 or M2 macrophages, and the macrophage-derived EVs (M0-EVs and M2-EVs, respectively) were collected and identified. The M2-EVs stimulation augmented proliferation, mobility, and the in vivo tumorigenic activity of CRC cells...
August 2023: Cytotechnology
https://read.qxmd.com/read/37389128/bioinformatics-analysis-of-synovial-fluid-derived-mesenchymal-stem-cells-in-the-temporomandibular-joint-stimulated-with-il-1%C3%AE
#37
JOURNAL ARTICLE
Yiting Lou, Ran Tao, Xiaoyan Weng, Suzhen Sun, Yong Yang, Binbin Ying
The stimulation of interleukin-1β (IL-1β) is the risk factor for temporomandibular joint osteoarthritis (TMJOA). We aim to investigate IL-1β stimulation-related gene and signal pathways in synovial fluid-derived mesenchymal stem cells (SF-MSCs) inflammatory activation to predict the occurrence of TMJOA. The microarray dataset GSE150057 was downloaded from the gene expression omnibus (GEO) database, and principal component analysis (PCA) was performed on the involved genes to obtain differential genes (DEGs)...
August 2023: Cytotechnology
https://read.qxmd.com/read/37389127/dehp-exposure-impairs-human-skeletal-muscle-cell-proliferation-in-primary-culture-conditions-preliminary-study
#38
JOURNAL ARTICLE
Elizabeth Brassea-Pérez, Vanessa Labrada-Martagón, Claudia J Hernández-Camacho, Ramón Gaxiola-Robles, José Pablo Vázquez-Medina, Tania Zenteno-Savín
The plasticizer di (2-ethylhexyl) phthalate (DEHP) inhibits differentiation, impairs glucose metabolism, and decreases mitochondrial function in murine muscle satellite cells; however, if these effects are translated to human cells is unknown. The goal of this study was to evaluate changes in morphology and proliferation of primary human skeletal muscle cells exposed to DEHP. Rectus abdominis muscle samples were obtained from healthy women undergoing programed cesarean surgery. Skeletal muscle cells were isolated and grown under standard primary culture conditions, generating two independent sample groups of 25 subcultures each...
August 2023: Cytotechnology
https://read.qxmd.com/read/37187949/targeting-a-novel-circitch-mir-421-btg1-axis-is-effective-to-suppress-the-malignant-phenotypes-in-hepatocellular-carcinoma-hcc-cells
#39
JOURNAL ARTICLE
Xiaodong Li, Xuedong Yin, Heyi Bao, Chang Liu
Circular RNA-based competing endogenous RNA (ceRNA) networks contribute to the initiation and development of various types of cancer, including hepatocellular carcinoma (HCC). Although a novel circular RNA itchy E3 ubiquitin protein ligase (circITCH) is identified as a tumor suppressor in HCC, its detailed molecular mechanisms have not been fully delineated. The present study was designed to resolve this issue, and we firstly verified that circITCH suppressed the malignant phenotypes in HCC cells by regulating a novel miR-421/B-cell translocation gene 1 (BTG1) axis...
June 2023: Cytotechnology
https://read.qxmd.com/read/37187948/molecular-chaperones-hsp40-hsp70-stip1-and-hsp90-are-involved-in-stabilization-of-cx43
#40
JOURNAL ARTICLE
Li An, Hong Gao, Yi Zhong, Yanqiu Liu, Ying Cao, Jing Yi, Xiang Huang, Chunlei Wen, Rui Tong, Zhijun Pan, Xu Yan, Meiyan Liu, Shengzhao Wang, Xue Bai, Hao Wu, Tingju Hu
To investigate the involvement of stress induced phosphoprotein 1 (STIP1), heat shock protein (HSP) 70, and HSP90 in ubiquitination of connexin 43 (Cx43) in rat H9c2 cardiomyocytes. Co-immunoprecipitation was used to detect protein-protein interactions and Cx43 ubiquitination. Immunofluorescence was used for protein co-localization. The protein binding, Cx43 protein expression, and Cx43 ubiquitination were reanalyzed in H9c2 cells with modified STIP1 and/or HSP90 expression. STIP1 bound to HSP70 and HSP90, and Cx43 bound to HSP40, HSP70, and HSP90 in normal H9c2 cardiomyocytes...
June 2023: Cytotechnology
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