Li An, Hong Gao, Yi Zhong, Yanqiu Liu, Ying Cao, Jing Yi, Xiang Huang, Chunlei Wen, Rui Tong, Zhijun Pan, Xu Yan, Meiyan Liu, Shengzhao Wang, Xue Bai, Hao Wu, Tingju Hu
To investigate the involvement of stress induced phosphoprotein 1 (STIP1), heat shock protein (HSP) 70, and HSP90 in ubiquitination of connexin 43 (Cx43) in rat H9c2 cardiomyocytes. Co-immunoprecipitation was used to detect protein-protein interactions and Cx43 ubiquitination. Immunofluorescence was used for protein co-localization. The protein binding, Cx43 protein expression, and Cx43 ubiquitination were reanalyzed in H9c2 cells with modified STIP1 and/or HSP90 expression. STIP1 bound to HSP70 and HSP90, and Cx43 bound to HSP40, HSP70, and HSP90 in normal H9c2 cardiomyocytes...
June 2023: Cytotechnology