journal
Journals FASEB Journal : Official Publi...

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology

https://read.qxmd.com/read/38776246/emerging-concepts-of-myosin-18a-isoform-mechanobiology-in-organismal-and-immune-system-physiology-development-and-function
#1
REVIEW
William DeKryger, Zissis C Chroneos
Alternative and combinatorial splicing of myosin 18A (MYO18A) gene transcripts results in expression of MYO18A protein isoforms and isoform variants with different membrane and subcellular localizations, and functional properties. MYO18A proteins are members of the myosin superfamily consisting of a myosin-like motor domain, an IQ motif, and a coiled-coil domain. MYO18A isoforms, however, lack the ability to hydrolyze ATP and do not perform ATP-dependent motor activity. MYO18A isoforms are distinguished by different amino- and carboxy-terminal extensions and domains...
May 31, 2024: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/38775929/exploring-mitochondrial-dna-copy-number-in-circulating-cell-free-dna-and-extracellular-vesicles-across-cardiovascular-health-status-a-prospective-case-control-pilot-study
#2
JOURNAL ARTICLE
Chiara Rucci, Gaia de Simone, Saniya Salathia, Cristina Casadidio, Roberta Censi, Laura Bordoni
Cardiovascular disease (CVD) is a leading global cause of mortality, difficult to predict in advance. Evidence indicates that the copy number of mitochondrial DNA (mtDNAcn) in blood is altered in individuals with CVD. MtDNA released into circulation may act as a mediator of inflammation, a recognized factor in the development of CVD, in the long distance. This pilot study aims to test if levels of mtDNAcn in buffy coat DNA (BC-mtDNA), in circulating cellfree DNA (cf-mtDNA), or in DNA extracted from plasma extracellular vesicles (EV-mtDNA) are altered in CVD patients and if they can predict heart attack in advance...
May 31, 2024: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/38775797/knockdown-of-lap2%C3%AE-inhibits-adipogenesis-of-human-adipose-derived-stem-cells-and-ameliorates-high-fat-diet-induced-obesity
#3
JOURNAL ARTICLE
Hang Gu, Yuan Pan, Han Xiao, Lijun Zhao, Yiman Tang, Wenshu Ge
Adipogenesis, a pivotal cellular process involving the differentiation of mesenchymal stem cells (MSCs) to mature adipocytes, plays a significant role in various physiological functions. Dysregulation of adipogenesis is implicated in conditions such as obesity. However, the complete molecular understanding of adipogenesis remains elusive. This study aimed to uncover the novel role of lamina-associated polypeptide 2 alpha (LAP2α) in human adipose-derived stem cells (hASCs) adipogenesis and its impact on high-fat diet (HFD)-induced obesity and associated metabolic disturbances...
May 31, 2024: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/38775792/dihydroartemisinin-alleviates-doxorubicin-induced-cardiotoxicity-and-ferroptosis-by-activating-nrf2-and-regulating-autophagy
#4
JOURNAL ARTICLE
Zhi-Hui Lin, Hua-Qiang Xiang, Yong-Wei Yu, Yang-Jing Xue, Chang Wu, Cong Lin, Kang-Ting Ji
Although the use of Doxorubicin (Dox) is extensive in the treatment of malignant tumor, the toxic effects of Dox on the heart can cause myocardial injury. Therefore, it is necessary to find an alternative drug to alleviate the Dox-induced cardiotoxicity. Dihydroartemisinin (DHA) is a semisynthetic derivative of artemisinin, which is an active ingredient of Artemisia annua. The study investigates the effects of DHA on doxorubicin-induced cardiotoxicity and ferroptosis, which are related to the activation of Nrf2 and the regulation of autophagy...
May 31, 2024: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/38767449/mitochondria-transplantation-alleviates-cardiomyocytes-apoptosis-through-inhibiting-ampk%C3%AE-mtor-mediated-excessive-autophagy
#5
JOURNAL ARTICLE
Ning Jin, Mengyao Zhang, Li Zhou, Shanshan Jin, Haiqin Cheng, Xuewei Li, Yaqian Shi, Tong Xiang, Zongxiao Zhang, Zhizhen Liu, Hong Zhao, Jun Xie
The disruption of mitochondria homeostasis can impair the contractile function of cardiomyocytes, leading to cardiac dysfunction and an increased risk of heart failure. This study introduces a pioneering therapeutic strategy employing mitochondria derived from human umbilical cord mesenchymal stem cells (hu-MSC) (MSC-Mito) for heart failure treatment. Initially, we isolated MSC-Mito, confirming their functionality. Subsequently, we monitored the process of single mitochondria transplantation into recipient cells and observed a time-dependent uptake of mitochondria in vivo...
May 31, 2024: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/38758186/sec-a-core-hub-during-cell-fate-alteration
#6
REVIEW
Zhijing Zhang, Jingyi Xu, Jiqiang Liu, Jiaqiang Wang, Lei Lei
Pol II pause release is a rate-limiting step in gene transcription, influencing various cell fate alterations. Numerous proteins orchestrate Pol II pause release, thereby playing pivotal roles in the intricate process of cellular fate modulation. Super elongation complex (SEC), a large assembly comprising diverse protein components, has garnered attention due to its emerging significance in orchestrating physiological and pathological cellular identity changes by regulating the transcription of crucial genes...
May 31, 2024: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/38758184/mesenchymal-stromal-cells-protect-tissues-from-th1-immune-responses-via-il-11-secretion
#7
JOURNAL ARTICLE
WonKyung J Cho, Vinay K Pulimamidi, Sharad K Mittal, Sunil K Chauhan
Mesenchymal stromal cells (MSCs) have been shown to modulate the function of various subsets of T cells such as naïve CD4+ T cells and IFNγ+ CD4+ Th1 cells; however, mechanisms underlying this regulation have not been fully deciphered. Our in vitro culture assays demonstrate that MSCs suppress the activation and function of CD4+ T cells by secreting interleukin 11, and neutralization of IL11 abrogates MSC-mediated suppression of CD4+ T cell function. Moreover, delayed-type, exogenous supplementation of IL11 significantly suppressed IFNγ+ expression by Th1 cells...
May 31, 2024: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/38752683/the-role-of-yap-taz-on-joint-and-arthritis
#8
REVIEW
Mingcheng Lu, Mengqi Zhu, Zuping Wu, Wei Liu, Chuwen Cao, Jiejun Shi
Osteoarthritis (OA) and rheumatoid arthritis (RA) are two common forms of arthritis with undefined etiology and pathogenesis. Yes-associated protein (YAP) and its homolog transcriptional coactivator with PDZ-binding motif (TAZ), which act as sensors for cellular mechanical and inflammatory cues, have been identified as crucial players in the regulation of joint homeostasis. Current studies also reveal a significant association between YAP/TAZ and the pathogenesis of OA and RA. The objective of this review is to elucidate the impact of YAP/TAZ on different joint tissues and to provide inspiration for further studying the potential therapeutic implications of YAP/TAZ on arthritis...
May 31, 2024: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/38752545/c-cbl-induced-podocin-ubiquitination-contributes-to-the-podocytes-injury-in-diabetic-nephropathy
#9
JOURNAL ARTICLE
Lulu Liang, Mengfei He, Panpan Zhou, Shaokang Pan, Jingfang Chen, Linxiao Lv, Mingyang Hu, Sijie Zhou, Dongwei Liu, Zhangsuo Liu
The ubiquitination function in diabetic nephropathy (DN) has attracted much attention, but there is a lack of information on its ubiquitylome profile. To examine the differences in protein content and ubiquitination in the kidney between db/db mice and db/m mice, we deployed liquid chromatography-mass spectrometry (LC-MS/MS) to conduct analysis. We determined 145 sites in 86 upregulated modified proteins and 66 sites in 49 downregulated modified proteins at the ubiquitinated level. Moreover, 347 sites among the 319 modified proteins were present only in the db/db mouse kidneys, while 213 sites among the 199 modified proteins were present only in the db/m mouse kidneys...
May 31, 2024: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/38752538/inhibition-of-nlrp3-inflammasome-by-mcc950-under-hypoxia-alleviates-photoreceptor-apoptosis-via-inducing-autophagy-in-m%C3%A3-ller-glia
#10
JOURNAL ARTICLE
Shuang Gao, Na Li, Zhongjing Lin, Yisheng Zhong, Yanuo Wang, Xi Shen
NLRP3 inflammasome activation has emerged as a critical initiator of inflammatory response in ischemic retinopathy. Here, we identified the effect of a potent, selective NLRP3 inhibitor, MCC950, on autophagy and apoptosis under hypoxia. Neonatal mice were exposed to hyperoxia for 5 days to establish oxygen-induced retinopathy (OIR) model. Intravitreal injection of MCC950 was given, and then autophagy and apoptosis markers were assessed. Retinal autophagy, apoptosis, and related pathways were evaluated by western blot, immunofluorescent labeling, transmission electron microscopy, and TUNEL assay...
May 31, 2024: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/38752537/ddx58-variant-triggers-ifn-%C3%AE-induced-autophagy-in-trabecular-meshwork-and-influences-intraocular-pressure
#11
JOURNAL ARTICLE
Xinting Huang, Xiaoyu Zhou, Feng Zhang, Xiaobo Wang, Xuanchu Duan, Ke Liu
Singleton-Merten syndrome (SMS) is a rare immunogenetic disorder affecting multiple systems, characterized by dental dysplasia, aortic calcification, glaucoma, skeletal abnormalities, and psoriasis. Glaucoma, a key feature of both classical and atypical SMS, remains poorly understood in terms of its molecular mechanism caused by DDX58 mutation. This study presented a novel DDX58 variant (c.1649A>C [p.Asp550Ala]) in a family with childhood glaucoma. Functional analysis showed that DDX58 variant caused an increase in IFN-stimulated gene expression and high IFN-β-based type-I IFN...
May 31, 2024: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/38752523/susceptibility-to-recombinant-sars-cov-2-spike-protein-entry-in-the-lungs-of-high-fat-diet-induced-obese-mice
#12
JOURNAL ARTICLE
Koro Gotoh, Fumiko Satoh, Honami Uesugi, Takaaki Noguchi, Naoki Matsuda, Kentaro Sada, Shotaro Miyamoto, Yoshinori Ozeki, Yuichi Yoshida, Mitsuhiro Okamoto, Takayuki Masaki, Toshiaki Kawabe, Hirotaka Shibata
Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Obesity is a major risk factor for the development of COVID-19. Angiotensin-converting enzyme 2 (ACE2) is an essential receptor for cell entry of SARS-CoV-2. The receptor-binding domain of the S1 subunit (S1-RBD protein) in the SARS-CoV-2 spike glycoprotein binds to ACE2 on host cells, through which the virus enters several organs, including the lungs. Considering these findings, recombinant ACE2 might be utilized as a decoy protein to attenuate SARS-CoV-2 infection...
May 31, 2024: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/38747803/impact-of-heparanase-2-hpa2-on-cancer-and-inflammation-advances-and-paradigms
#13
REVIEW
Israel Vlodavsky, Maram Hilwi, Yasmin Kayal, Soaad Soboh, Neta Ilan
HPSE2, the gene-encoding heparanase 2 (Hpa2), is mutated in urofacial syndrome (UFS), a rare autosomal recessive congenital disease attributed to peripheral neuropathy. Hpa2 lacks intrinsic heparan sulfate (HS)-degrading activity, the hallmark of heparanase (Hpa1), yet it exhibits a high affinity toward HS, thereby inhibiting Hpa1 enzymatic activity. Hpa2 regulates selected genes that promote normal differentiation, tissue homeostasis, and endoplasmic reticulum (ER) stress, resulting in antitumor, antiangiogenic, and anti-inflammatory effects...
May 31, 2024: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/38747734/lactobacillus-casei-fermented-amomum-xanthioides-ameliorates-metabolic-dysfunction-in-high-fat-diet-induced-obese-mice
#14
JOURNAL ARTICLE
Jing-Hua Wang, Seung-Ju Hwang, Yu-Jin Choi, Tae-Wook Woo, Chang-Gue Son
Amomum xanthioides (AX) has been used as an edible herbal medicine to treat digestive system disorders in Asia. Additionally, Lactobacillus casei is a well-known probiotic commonly used in fermentation processes as a starter. The current study aimed to investigate the potential of Lactobacillus casei-fermented Amomum xanthioides (LAX) in alleviating metabolic disorders induced by high-fat diet (HFD) in a mouse model. LAX significantly reduced the body and fat weight, outperforming AX, yet without suppressing appetite...
May 31, 2024: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/38742812/complanatuside-a-ameliorates-2-4-6-trinitrobenzene-sulfonic-acid-induced-colitis-in-mice-by-regulating-the-th17-treg-balance-via-the-jak2-stat3-signaling-pathway
#15
JOURNAL ARTICLE
Yueyue Wang, Xue Song, Yongsheng Xia, Wenjing Zhang, Wenjie Li, Yu Wang, Jing Li, Zhijun Geng, Xiaofeng Zhang, Lian Wang, Lugen Zuo, Jianguo Hu
Immunity imbalance of T helper 17 (Th17)/regulatory T (Treg) cells is involved in the pathogenesis of Crohn's disease (CD). Complanatuside A (CA), a flavonol glycoside, exerts anti-inflammatory activities and our study aimed to identify its effect on TNBS-induced colitis and the possible mechanisms. We found that CA alleviated the symptoms of colitis in TNBS mice, as demonstrated by prevented weight loss and colon length shortening, as well as decreased disease activity index scores, inflammatory scores, and levels of proinflammatory factors...
May 31, 2024: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/38742811/mir-4645-3p-attenuates-podocyte-injury-and-mitochondrial-dysfunction-in-diabetic-kidney-disease-by-targeting-cdk5
#16
JOURNAL ARTICLE
Yue Zhang, Shunjie Xia, Xiaoxi Tian, Liming Yuan, Yuan Gao, Dan Liu, Huimin Qi, Shuo Wang, Zanchao Liu, Yang Li, Zhe Zhao, Wei Liu
Podocyte injury plays a critical role in the progression of diabetic kidney disease (DKD), but the underlying cellular and molecular mechanisms remain poorly understanding. MicroRNAs (miRNAs) can disrupt gene expression by inducing translation inhibition and mRNA degradation, and recent evidence has shown that miRNAs may play a key role in many kidney diseases. In this study, we identified miR-4645-3p by global transcriptome expression profiling as one of the major downregulated miRNAs in high glucose-cultured podocytes...
May 31, 2024: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/38742809/whole-transcriptome-sequencing-reveals-the-activity-of-the-pla2-family-members-in-androctonus-crassicauda-scorpionida-buthidae-venom-gland
#17
JOURNAL ARTICLE
Fatemeh Salabi, Hedieh Jafari
Phospholipase A2 is the most abundant venom gland enzyme, whose activity leads to the activation of the inflammatory response by accumulating lipid mediators. This study aimed to identify, classify, and investigate the properties of venom PLA2 isoforms. Then, the present findings were confirmed by chemically measuring the activity of PLA2. The sequences representing PLA2 annotation were extracted from the Androctonus crassicauda transcriptome dataset using BLAS searches against the local PLA2 database. We found several cDNA sequences of PLA2 classified and named by conducting multiple searches as platelet-activating factor acetylhydrolases, calcium-dependent PLA2s, calcium-independent PLA2s, and secreted PLA2s...
May 31, 2024: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/38742798/biosensor-capability-of-the-endometrium-is-mediated-in-part-by-altered-mirna-cargo-from-conceptus-derived-extracellular-vesicles
#18
JOURNAL ARTICLE
Tiago H C De Bem, Alessandra Bridi, Haidee Tinning, Rafael Vilar Sampaio, Irene Malo-Estepa, Dapeng Wang, Elton J R Vasconcelos, Ricardo Perecin Nociti, Ana C F C M de Ávila, Juliano Rodrigues Sangalli, Igor Garcia Motta, Gilmar Arantes Ataíde, Júlio C B da Silva, Yeda Fumie Watanabe, Angela Gonella-Diaza, Juliano C da Silveira, Guilherme Pugliesi, Flávio Vieira Meirelles, Niamh Forde
We tested the hypothesis that the biosensor capability of the endometrium is mediated in part, by the effect of different cargo contained in the extracellular vesicles secreted by the conceptus during the peri-implantation period of pregnancy. We transferred Bos taurus taurus embryos of different origin, in vivo (high developmental potential (IV)), in vitro (intermediate developmental potential (IVF)), or cloned (low developmental potential (NT)), into Bos taurus indicus recipients. Extracellular vesicles (EVs) recovered from Day 16 conceptus-conditioned medium were characterized and their microRNA (miRNA) cargo sequenced alongside RNA sequencing of their respective endometria...
May 31, 2024: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/38742770/single-nucleus-and-spatial-transcriptomic-profiling-of-healthy-human-hamstring-tendon
#19
JOURNAL ARTICLE
Jolet Y Mimpen, Lorenzo Ramos-Mucci, Claudia Paul, Alina Kurjan, Philippa A Hulley, Chinemerem T Ikwuanusi, Carla J Cohen, Stephen E Gwilym, Mathew J Baldwin, Adam P Cribbs, Sarah J B Snelling
The molecular and cellular basis of health in human tendons remains poorly understood. Among human tendons, hamstring tendon has markedly low pathology and can provide a prototypic healthy tendon reference. The aim of this study was to determine the transcriptomes and location of all cell types in healthy hamstring tendon. Using single nucleus RNA sequencing, we profiled the transcriptomes of 10 533 nuclei from four healthy donors and identified 12 distinct cell types. We confirmed the presence of two fibroblast cell types, endothelial cells, mural cells, and immune cells, and identified cell types previously unreported in tendons, including different skeletal muscle cell types, satellite cells, adipocytes, and undefined nervous system cells...
May 31, 2024: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/38739537/mechanical-stretch-preconditioned-adipose-derived-stem-cells-elicit-polarization-of-anti-inflammatory-m2-like-macrophages-and-improve-chronic-wound-healing
#20
JOURNAL ARTICLE
Jiahao He, Shengzhou Shan, Taoran Jiang, Sizheng Zhou, Jiaqi Qin, Qingfeng Li, Zheyuan Yu, Dejun Cao, Bin Fang
Transplantation of adipose-derived stem cells (ASCs) is a promising option in the field of chronic wounds treatment. However, the effectiveness of ASCs therapies has been hampered by highly inflammatory environment in chronic wound areas. These problems could be partially circumvented using efficient approaches that boost the survival and anti-inflammatory capacity of transplanted ASCs. Here, by application of mechanical stretch (MS), we show that ASCs exhibits increased survival and immunoregulatory properties in vitro...
May 31, 2024: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
journal
journal
29811
1
2
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.