Ying Bai, Liz Bentley, Chao Ma, Navaratnam Naveenan, James Cleak, Yixing Wu, Michelle M Simon, Henrik Westerberg, Ramón Casero Cañas, Neil Horner, Rajesh Pandey, Keanu Paphiti, Ulrike Schulze, Joffrey Mianné, Tertius Hough, Lydia Teboul, Jeroen H F de Baaij, Roger D Cox
ARL15, a small GTPase protein, was linked to metabolic traits in association studies. We aimed to test the Arl15 gene as a functional candidate for metabolic traits in the mouse. CRISPR/Cas9 germline knockout (KO) of Arl15 showed that homozygotes were postnatal lethal and exhibited a complete cleft palate (CP). Also, decreased cell migration was observed from Arl15 KO mouse embryonic fibroblasts (MEFs). Metabolic phenotyping of heterozygotes showed that females had reduced fat mass on a chow diet from 14 weeks of age...
November 2023: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology