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European Journal of Clinical Microbiology & Infectious Diseases

Laura Hidalgo, Fernando Salvador, Elena Sulleiro, Isabel López, Martha Balladares, Elena García, Carmen Paz, Adrián Sánchez-Montalvá, Pau Bosch-Nicolau, Augusto Sao-Avilés, Israel Molina
Blastocystis sp. is the most common intestinal parasite isolated in humans. The aim of the study was to describe the risk factors associated to Blastocystis sp. detection. A case-control retrospective study was carried out at Vall d'Hebron University Hospital (Barcelona, Spain), which receives all fecal samples collected in Barcelona at primary care level. Eligible patients were patients older than 18 years in whom three consecutive stool samples were examined for parasitic diagnosis from January to December 2017...
March 16, 2019: European Journal of Clinical Microbiology & Infectious Diseases
Linda Mueller, Cansu Cimen, Laurent Poirel, Marie-Christine Descombes, Patrice Nordmann
Our aim was to evaluate the prevalence of fosfomycin-resistant strains among ESBL-producing Escherichia coli isolates recovered from community patients in Switzerland. A total of 1225 ESBL-producing E. coli isolates were collected between 2012 and 2013 from a private and community laboratory. Fosfomycin resistance was assessed by using the novel rapid fosfomycin/E. coli NP test and agar dilution method. Resistant isolates were further investigated for acquired resistance genes fosA1-7 by PCR and sequencing...
March 15, 2019: European Journal of Clinical Microbiology & Infectious Diseases
Si-Ho Kim, Suhyun Oh, Kyungmin Huh, Sun Young Cho, Cheol-In Kang, Doo Ryeon Chung, Kyong Ran Peck
Extended-spectrum β-lactamase-producing Enterobacteriales (ESBL-PE) are often associated with inappropriate empirical therapy (IAT). The aim of this study was to investigate whether IAT of acute pyelonephritis (APN) caused by ESBL-PE is related to adverse outcomes. A retrospective cohort study was performed at a tertiary-care hospital from 2014 through 2016. Patients who had APN caused by ESBL-PE and were definitely treated with appropriate antibiotics for at least 7 days were enrolled. IAT was defined as when inappropriate empirical antibiotics were given 48 h or longer after initial diagnosis of APN...
March 13, 2019: European Journal of Clinical Microbiology & Infectious Diseases
Naomi J Gadsby, Martin P McHugh, Callum Forbes, Laura MacKenzie, Stephen K D Hamilton, David M Griffith, Kate E Templeton
Faster respiratory pathogen detection and antibiotic resistance identification are important in critical care due to the severity of illness, significant prior antibiotic exposure and infection control implications. Our objective was to compare the performance of the commercial Unyvero P55 Pneumonia Cartridge (Curetis AG) with routine bacterial culture methods and in-house bacterial multiplex real-time PCR assays. Seventy-four bronchoalveolar lavage specimens from patients admitted to a Scottish intensive care unit (ICU) over a 33-month period were tested prospectively by routine culture and viral PCR and retrospectively by Unyvero P55 and in-house bacterial PCR...
March 11, 2019: European Journal of Clinical Microbiology & Infectious Diseases
Maya Azrad, Elena Danilov, Sivan Goshen, Orna Nitzan, Avi Peretz
We compared the performance of two rapid antigen tests-QuikRead go® Strep A test (Orion Diagnostica, Espoo, Finland) and BD Veritor™ system (Becton, Dickinson and Company, Sparks, MD) with throat culture. Our aim was to evaluate each assay's performance and agreement compared to throat culture in order to choose one of the assays as a point-of-care test in the emergency room. One hundred throat samples were collected in triplicates from patients with suspected pharyngitis admitted to the emergency room. One throat swab was seeded for a throat culture...
March 9, 2019: European Journal of Clinical Microbiology & Infectious Diseases
Saskia J Bogers, Frederike V van Daalen, Sacha D Kuil, Menno D de Jong, Suzanne E Geerlings
The appropriate use of microbiological investigations is an important cornerstone of antibiotic stewardship programmes, but receives relatively limited attention. This study aimed to identify influencing factors in performing microbiological diagnostic tests and to assess the need for a clinical guideline. We performed a qualitative (focus group) and quantitative (online questionnaire survey) study among medical specialists and residents to identify physicians' considerations in performing microbiological diagnostic tests and to assess the need for a diagnostic guideline...
March 7, 2019: European Journal of Clinical Microbiology & Infectious Diseases
Milo Gatti, Laura Elisa Gasparini, Matteo Laratta, Anna Sigurtà, Anna Rossi, Paolo Brioschi, Osvaldo Chiara, Chiara Vismara, Francesco Scaglione, Sergio Arlati
To illustrate the effectiveness of our intensive multidisciplinary management (IMM) in the treatment of severely ill patients with necrotizing soft tissue infections (NSTIs). A retrospective observational study was conducted in a general ICU. Thirty-two consecutive patients undergoing IMM were carefully compared with 30 consecutive patients receiving a standard management (SM). IMM combined intensive care management, early surgical debridement followed by daily inspection of surgical wounds, close microbiological surveillance, and targeted high-dose antibiotics...
March 6, 2019: European Journal of Clinical Microbiology & Infectious Diseases
Stefan A Boers, Ruud Jansen, John P Hays
Recent advancements in next-generation sequencing (NGS) have provided the foundation for modern studies into the composition of microbial communities. The use of these NGS methods allows for the detection and identification of ('difficult-to-culture') microorganisms using a culture-independent strategy. In the field of routine clinical diagnostics however, the application of NGS is currently limited to microbial strain typing for epidemiological purposes only, even though the implementation of NGS for microbial community analysis may yield clinically important information...
March 5, 2019: European Journal of Clinical Microbiology & Infectious Diseases
Chiara Della Bella, Elisabetta Venturini, Savannah Devente, Paola Piccini, Simona Tapinassi, Leila Bianchi, Alessia Grassi, Marisa Benagiano, Heba F Mustafa Alnwaisri, Carlotta Montagnani, Elena Chiappini, Wilbert Bitter, Mario M D'Elios, Maurizio de Martino, Luisa Galli
Nontuberculous mycobacteria are the most frequent cause of chronic cervical lymphadenitis in childhood. The aim of the study was to evaluate the performance of IL-2, IL-17, and INF-γ in-house enzyme-linked immunospot assays using a Mycobacterium avium lysate, in order to identify a noninvasive diagnostic method of nontuberculous mycobacteria infection. Children with subacute and chronic lymphadenopathies or with a previous diagnosis of nontuberculous mycobacteria lymphadenitis were prospectively enrolled in the study...
March 5, 2019: European Journal of Clinical Microbiology & Infectious Diseases
Juan Carlos Rodríguez, Emilio Borrajo, Montserrat Bosque, Juan José Camarena, Javier Colomina, Ma Victoria Domínguez Márquez, Encarnación Fuentes, José María García-Aguayo, Adelina Gimeno, Nieves Gonzalo, Remedios Guna, Olalla Martínez, Rosario Moreno, José Miguel Nogueira, Nieves Orta, Josep Prat, Alberto Yagüe, Concepción Gimeno, David Navarro
No abstract text is available yet for this article.
March 2, 2019: European Journal of Clinical Microbiology & Infectious Diseases
Céline Slekovec, Jérôme Robert, Nathalie van der Mee-Marquet, Philippe Berthelot, Anne-Marie Rogues, Véronique Derouin, Pascal Cholley, Michelle Thouverez, Didier Hocquet, Xavier Bertrand
Although Pseudomonas aeruginosa has a non-clonal epidemic population structure, recent studies have provided evidence of the existence of epidemic high-risk clones. The aim of this study was to assess the molecular epidemiology of P. aeruginosa isolates responsible for infections in French ICUs, regardless of resistance patterns. For a 1-year period, all non-duplicate P. aeruginosa isolated from bacteremia and pulmonary infections in ten adult ICUs of six French university hospitals were characterized by antimicrobial susceptibility testing and genotyping (MLST and PFGE)...
March 2, 2019: European Journal of Clinical Microbiology & Infectious Diseases
Irene Galani, Maria Souli, Konstantina Nafplioti, Panagiora Adamou, Ilias Karaiskos, Helen Giamarellou, Anastasia Antoniadou
Relebactam is a β-lactamase inhibitor of class A and class C β-lactamases, including carbapenemases. We evaluated the ability of relebactam to restore imipenem susceptibility against a collection of Klebsiella pneumoniae isolates from Greek hospitals. We tested 314 non-MBL carbapenemase-producing K. pneumoniae consecutive clinical strains isolated from unique patients at 18 hospitals in Greece, between November 2014 and December 2016. Susceptibility testing of imipenem, imipenem-relebactam, meropenem, doripenem, gentamicin, and colistin was performed using broth microdilution...
March 1, 2019: European Journal of Clinical Microbiology & Infectious Diseases
Antonio Lalueza, Dolores Folgueira, Irene Muñoz-Gallego, Hernando Trujillo, Jaime Laureiro, Pilar Hernández-Jiménez, Noelia Moral-Jiménez, Cristina Castillo, Blanca Ayuso, Carmen Díaz-Pedroche, Marta Torres, Estibaliz Arrieta, Coral Arévalo-Cañas, Olaya Madrid, Carlos Lumbreras
The role of viral load in the outcome of patients requiring hospital admission due to influenza is not well established. We aim to assess if there is an association between the viral load and the outcome in hospitalized patients with a confirmed influenza virus infection. A retrospective observational study including all adult patients who were hospitalized in our center with a confirmed influenza virus infection from January to May 2016. Viral load was measured by real-time reverse-transcriptase-polymerase chain reaction (rRT-PCR) cycle threshold (Ct) value on upper respiratory tract samples...
February 28, 2019: European Journal of Clinical Microbiology & Infectious Diseases
Despoina Koulenti, Francesca Romana Parisella, Elena Xu, Jeffrey Lipman, Jordi Rello
Unfortunately, the Acknowledgements section was not included in the original version of the article. The said section is given here.
February 28, 2019: European Journal of Clinical Microbiology & Infectious Diseases
Vu Thi Nga, Tran Uyen Ngoc, Le Bui Minh, Vo Truong Nhu Ngoc, Van-Huy Pham, Le Long Nghia, Nguyen Lan Hung Son, Thi Hong Van Pham, Nguyen Duy Bac, Tran Viet Tien, Nguyen Ngoc Minh Tuan, Yang Tao, Pau Loke Show, Dinh-Toi Chu
In recent decades, exceeding 60% of infectious cases in human beings are originated from pathogenic agents related to feral or companion animals. This figure continues to swiftly increase due to excessive exposure between human and contaminated hosts by means of applying unhygienic farming practices throughout society. In Asia countries-renowned for lax regulation towards animal-trading markets-have experienced tremendous outbreaks of zoonotic diseases every year. Meanwhile, various epidemic surges were first reported in the residential area of China-one of the largest distributor of all animal products on the planet...
February 26, 2019: European Journal of Clinical Microbiology & Infectious Diseases
Adam L Bailey, Carey-Ann D Burnham
In order to realize the full potential of total laboratory automation (TLA) in the clinical microbiology laboratory, workflows must be optimized to match each laboratory's capabilities, patient population, and staffing model. Using TLA-based digital photography to monitor urine cultures, we sought to improve culture result turn-around-time (TAT) by changing the time at which a culture is first photographed and thus available for analysis/work-up (Pre1) from 18 h (16,391 cultures) to 16 h (53,113 cultures) (with a total of 24-h culture incubation in both time periods); in both time periods, cultures were set up 24/7, and culture work-up occurred during the day shift only...
February 26, 2019: European Journal of Clinical Microbiology & Infectious Diseases
Junpeng Zhao, Le Chang, Lunan Wang
Significant advances have been made in the molecular assays used for the detection of human immunodeficiency virus (HIV), which are crucial in preventing HIV transmission and monitoring disease progression. Molecular assays for HIV diagnosis have now reached a high degree of specificity, sensitivity and reproducibility, and have less operator involvement to minimize risk of contamination. Furthermore, analyses have been developed for the characterization of host gene polymorphisms and host responses to better identify and monitor HIV-1 infections in the clinic...
February 23, 2019: European Journal of Clinical Microbiology & Infectious Diseases
E Sogodogo, M Drancourt, Ghiles Grine
Methanogens are strictly anaerobic archaea metabolising by-products of bacterial fermentation into methane by using three known metabolic pathways, i.e. the reduction of carbon dioxide, the fermentation of acetate or the dismutation of methanol or methylamines. Methanogens described in human microbiota include only Euryarchaeota, i.e. Methanobrevibacter smithii, Methanobrevibacter oralis, Methanobrevibacter arbophilus, Methanobrevibacter massiliensis, Methanomassiliicoccus luminyensis, Methanosphaera stadtmanae and Ca...
February 22, 2019: European Journal of Clinical Microbiology & Infectious Diseases
Camila Freze Baez, Marianna Tavares Venceslau Gonçalves, Willker Menezes da Rocha, Leandro Magalhães de Souza, Flavia Savassi-Ribas, Núbia Karla de Oliveira Almeida, Serena Delbue, Maria Angelica Arpon Marandino Guimarães, Silvia Maria Baeta Cavalcanti, Flávio Barbosa Luz, Rafael Brandão Varella
Non-melanoma skin cancers (NMSC) share similar risk factors with other virus-related cancers, despite the lack of proved causal association between viral infection and NMSC development. We investigated the presence of Merkel cell polyomavirus (MCPyV), Epstein-Barr virus (EBV), and human papillomavirus (HPV) DNA in 83 NMSC fresh-frozen and 16 non-cancerous skin biopsies and evaluated viral infection according to demographical data, histopathological diagnosis, and ultraviolet exposure. Our results showed that 75% of NMSC biopsies were positive for at least one out of three viruses, whereas only 38% of non-cancerous skin biopsies were positive (p = 0...
February 21, 2019: European Journal of Clinical Microbiology & Infectious Diseases
Ellen H A van den Munckhof, Maurits N C de Koning, Wim G V Quint, Leen-Jan van Doorn, Maurine A Leverstein-van Hall
In clinical practice, the diagnosis of lower respiratory tract infections (LRTIs) is based on culture. The aim of this study was to evaluate whether a stepwise approach using microbiota analysis, species-specific quantitative real-time (q)PCRs and culture has the potential to be a more accurate and efficient diagnostic approach than culture alone. Sixty-two sputa obtained in a routine clinical setting from patients with a suspected LRTI were included. All sputa were analysed by culture, microbiota analysis based on the 16S ribosomal RNA gene and multiple species-specific qPCRs...
February 20, 2019: European Journal of Clinical Microbiology & Infectious Diseases
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