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Journal of Clinical Laboratory Analysis

Siwen Liang, Hongwei Li, Xuhua Shen, Ruifeng Liu
BACKGROUND: Previous studies suggested that adiponectin (APN) could ameliorate ischemia/reperfusion injury and endothelial dysfunction in patients with acute myocardial infarction. However, the relationship between serum APN level and coronary flow after primary percutaneous coronary intervention (PPCI) in patients with ST-segment elevation myocardial infarction (STEMI) is unclear. METHODS: A total of 144 patients with STEMI treated by PPCI were enrolled and divided into two groups based on the mean serum APN level on admission...
February 19, 2019: Journal of Clinical Laboratory Analysis
Ling Xue, Songyuan Luo, Huanyu Ding, Yuan Liu, Wenhui Huang, Xiaoping Fan, Min Wu, Xuhua Jian, Cheng Huang, Jianfang Luo, Ruixin Fan
BACKGROUND: This study aimed to investigate whether miR-146a-5p was involved in the pathogenesis of thoracic aortic dissection (AD) via regulating the biological function of vascular smooth muscle cells (VSMCs). METHODS: Circulating miR-146a-5p level was measured by quantitative polymerase chain reaction (qPCR) in AD patients and healthy controls. Human dissected aortic samples were obtained from patients with thoracic AD Stanford type A undergoing surgical repair, and normal control samples were from organ donors who died from nonvascular diseases...
February 19, 2019: Journal of Clinical Laboratory Analysis
Qian Liu, Jilong Fan, Aiguo Xu, Li Yao, Yan Li, Wenjun Wang, Wei Liang, Fumeng Yang
BACKGROUND: Neuron-specific enolase (NSE) is an important tumor marker in the serum of patients with lung cancer. Elevated serum NSE levels are also associated with many other diseases. However, there is no unified population reference interval for serum NSE. This study aimed to investigate the distribution of serum NSE in healthy Chinese adults aged 20-79 years and to establish its reference interval in Chinese population. METHODS: A total of 10 575 healthy subjects were in line with the requirements of this study...
February 19, 2019: Journal of Clinical Laboratory Analysis
Zhenhua Du, JiQin Liu, Hua Zhang, BuHe Bao, RuiQi Zhao, Ying Jin
BACKGROUND: In vitro hemolysis is still the most common source of pre-analytical nonconformities. This study aimed to investigate the hemolytic effects on commonly used biochemical tests as well as to determine the hemolysis index (HI) thresholds on Siemens Advia 2400 chemistry analyzer. METHODS: Peripheral blood samples were collected from forty healthy volunteers. Hemolysis was achieved using syringes. Five hemolysis levels were produced including the no hemolysis group, slight hemolysis group, mild hemolysis group, moderate hemolysis group, and heavy hemolysis group...
February 19, 2019: Journal of Clinical Laboratory Analysis
Li Zhou, Dong-Mei Xiao, Wen Qin, Bin-Hua Xie, Ting-Hui Wang, Hua Huang, Bao-Jing Zhao, Xi Han, Qing-Qing Sun, Xiu-Di Wu, Han Cen
BACKGROUND: Emerging evidence indicates that some hematological markers have critical value in evaluating treatment response. This study was performed to determine the clinical value of hemoglobin (Hb), platelet (Plt), neutrophil-to-lymphocyte ratio (NLR), and platelet-to-lymphocyte ratio (PLR) in rheumatoid arthritis (RA) patients treated with tocilizumab (TCZ). METHODS: Fifty-two RA patients receiving TCZ were recruited and followed for 6 months. The values of abovementioned hematological markers were collected...
February 19, 2019: Journal of Clinical Laboratory Analysis
Arno Floore, Albertus Hesselink, Anja Oštrbenk, Elia Alcaniz, Beate Rothe, Helle Pedersen, Montserrat Torres Hortal, Saskia Doorn, Wim Quint, Karl Ulrich Petry, Mario Poljak, Kate Cuschieri, Jesper Bonde, Silvia de Sanjosé, Maaike Bleeker, Daniëlle Heideman
BACKGROUND: HPV-based cervical screening detects women at an increased risk of cervical cancer and precancer. To differentiate among HPV-positive women those with (pre)cancer, triage testing is necessary. The detection of cancer-associated host-cell DNA methylation (FAM19A4 and hsa-mir124-2) in cervical samples has shown valuable as triage test. This multicenter study from 6 collaborating European laboratories and one reference laboratory was set out to determine the intra- and inter-laboratory agreement of FAM19A4/mir124-2 DNA methylation analysis utilizing the QIAsure Methylation Test...
February 13, 2019: Journal of Clinical Laboratory Analysis
Zi-Lan Huang, Pei-Pei Meng, Yun Yang, Sheng-Yu Wang, Xiu-Fang Zhang, Fang-Hong Luo, Jiang-Hua Yan, Ting Wu
BACKGROUND: Neuropilins (Nrps) are a new type of broad-spectrum tumor marker. Currently, a method for accurate simultaneous quantification of Nrps is not available. We aimed to develop a bead-based and duplexed flow cytometric assay that could be used for accurate and simultaneous quantification of Nrp1 and Nrp2 for scientific research or clinical diagnosis. METHODS: We coupled anti-human Nrp1-11# mAb and anti-human Nrp2-C3 mAb to magnetic beads 18# and 25#, respectively...
February 13, 2019: Journal of Clinical Laboratory Analysis
Fan Yu, Qianjin Bai, Shihong Zhang, Yongmei Jiang
OBJECTIVE: The purpose of the present study was to explore the predictive effects of soluble fms-like tyrosine kinase-1 (sFlt-1) and placental growth factor (PlGF) for preeclampsia. METHODS: A total of 1580 singleton pregnant women aged 18-45 years were included in this study. Serum samples were collected and stored frozen during their regular obstetric examinations. A total of 48 women who were eventually diagnosed with preeclampsia among them were defined as the preeclampsia group, other 134 women who were matched with age and sample collecting gestational weeks and finally diagnosed without preeclampsia were selected as control...
February 13, 2019: Journal of Clinical Laboratory Analysis
Tangyuheng Liu, Jiajia Song, Mei Zhang, Siyue Li, Jinya Zhang, Xuejiao Hu, Zhenzhen Zhao, Wu Peng, Qian Wu, Hao Bai, Yinyu Li, Xiaojun Lu, Binwu Ying
BACKGROUND: We investigated the relationship between hepatitis B virus (HBV)-related pathogenesis and single nucleotide polymorphisms (SNPs) in interleukin-21 (IL-21)-JAK-STAT signaling pathway genes. METHODS: We used the high-resolution melting (HRM) method to genotype five SNPs (IL-21 rs2221903, IL-21 rs4833837, IL-21 receptor (IL-21R) rs2285452, JAK3 rs3008, and STAT3 rs1053023) in 546 HBV-infected patients and 353 healthy Chinese subjects. The HBV-infected patients were further divided into subgroups based on the HBV-related pathologies: chronic hepatitis B (CHB), HBV-related liver cirrhosis (LC), and HBV-related hepatocellular carcinoma (HCC)...
February 13, 2019: Journal of Clinical Laboratory Analysis
Mansour Amin, Samaneh Yousef Pour, Tahereh Navidifar
INTRODUCTION: Empyema is one of the important causes of pediatric hospital admissions. AIM: In this study, we had investigated the frequency rates of S aureus, S pneumoniae, H influenzae, and P aeruginosa using PCR and bacterial culture among children suffering from empyema in Ahvaz city, Iran. METHODS: This was a descriptive study conducted on the patients hospitalized in ICUs of two teaching Hospitals of Ahvaz, Iran, between March and September 2018 on 105 pleural fluid (PF) samples of the children less than 16 years of age with the diagnosis of empyema thoracis...
February 9, 2019: Journal of Clinical Laboratory Analysis
You Lan, Mao Zhou, Zijuan Jian, Qun Yan, Siyi Wang, Wenen Liu
BACKGROUND: The emerging pks-positive (pks+ ) strains have aroused great public concern recently. Colibactin, encoded by pks gene cluster, has been reported to be involved in DNA damage and increased virulence. Little is known about its prevalence among Klebsiella pneumoniae-induced bloodstream infections (BSIs). Therefore, the aim of this study was to investigate the prevalence of pks gene cluster, and molecular and clinical characteristics of K pneumoniae-induced BSIs. METHODS: A total of 190 non-duplicate K pneumoniae bloodstream isolates were collected at a university hospital in China from March 2016 to March 2018...
February 8, 2019: Journal of Clinical Laboratory Analysis
Li Huang, Congying Chen, Lijuan Yang, Rong Wan, Guoyong Hu
OBJECTIVES: We aimed to evaluate the values of neutrophil-to-lymphocyte ratio (NLR) and white blood cell (WBC) in predicting severity of acute pancreatitis (AP) with different etiologies. METHODS: We compared NLR and WBC levels in patients with different etiologies and AP severity. The optimal cutoff value for them to predict severe acute pancreatitis (SAP) was determined by receiver operating characteristic (ROC) curve analysis. RESULTS: Both NLR and WBC were elevated in patients with SAP...
February 8, 2019: Journal of Clinical Laboratory Analysis
Danchen Wang, Songlin Yu, Yicong Yin, Shaowei Xie, Qian Cheng, Honglei Li, Xinqi Cheng, Ling Qiu
BACKGROUND: Iodine, an essential nutrient, is the most important trace element in thyroid hormone synthesis and maintenance of thyroid function. This study investigated the iodine nutrition status in healthy Chinese adults and assessed the relationship between urinary iodine concentration (UIC) and thyroid hormone levels. METHODS: A cross-sectional, multicenter study was conducted between October 2017 and January 2018, with 1017 adults recruited from five cities in China...
February 8, 2019: Journal of Clinical Laboratory Analysis
Uğur Aksu, Oktay Gulcu, Emrah Aksakal, Kamuran Kalkan, Mustafa Öztürk, Ali Fuat Korkmaz, Abdulkadir Uslu, Selami Demirelli
BACKGROUND: Although interventional methods are the first-line treatment options in ST-segment elevation myocardial infarction (STEMI), the incidence of stent restenosis (SR) is frequent. We investigated the relationship between CRP/albumin ratio (CAR), a novel indicator of inflammatory response, and SR in this study. METHOD: This study was carried out on the patients who underwent coronary angiography in our clinic between January 2017 and September 2017. Two groups were generated according to restenosis status (group 1: restenosis (-), group 2: restenosis (+)), and clinical biochemical and angiographical features were compared...
February 8, 2019: Journal of Clinical Laboratory Analysis
Chadarat Ampasavate, Wasimon Jutapakdee, Rungsinee Phongpradist, Singkome Tima, Adisak Tantiworawit, Pimlak Charoenkwan, Dujrudee Chinwong, Songyot Anuchapreeda
BACKGROUND: Overexpression of fms-like tyrosine kinase 3 (FLT3) protein in leukemia is highly related to poor prognosis and reduced survival rate in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL) patients. Simple but efficient quantification of FLT3 protein levels on the leukemic cell surface using flow cytometry had been developed for rapid determination of FLT3 on intact cell surface. METHODS: Quantitation protocol for FLT3 biomarker in clinical samples was developed and validated...
February 8, 2019: Journal of Clinical Laboratory Analysis
Ling Chen, Yue Qin, Tenglong Zhang, Ning Ding, Yi Chen, Zhe Zhang, Chengye Guo
BACKGROUND: To determine whether cancer-associated fibroblasts (CAFs) are associated with microvessel density (MVD) and lymphatic vessel density (LVD) in lung adenocarcinoma (ADC) or are not prognostic. METHODS: Ninety-three lung adenocarcinoma patients without adjuvant therapy between January 2010 and June 2011 were enrolled. CAFs, MVD, and LVD were identified by α-smooth muscle actin (α-SMA), CD34 and D2-40 staining via immunohistochemistry. Staining intensities were assessed and quantified...
February 8, 2019: Journal of Clinical Laboratory Analysis
Woo-Jae Kwoun, Jeong-Yeal Ahn, Pil-Whan Park, Yiel-Hea Seo, Kyung-Hee Kim, Ja Young Seo, Hwan Tae Lee, Kuai-Han Yoo
BACKGROUND: Bone marrow (BM) study plays an important role as initial investigation specimen of lymphoma as well as staging lymphoma. This study aimed to investigate the utility of BM studies for classification of lymphoma and evaluate features of BM involvement by lymphoma over a period of 11 years. METHODS: A total of 1162 cases of BM studies for lymphoma evaluation were reviewed for the incidence of lymphoma subtypes, the percentage of marrow involvement, the pattern of involvement and discordance with histopathologic diagnoses of lymph nodes and other tissues...
February 6, 2019: Journal of Clinical Laboratory Analysis
Jing Qiu, Hui Zhou, Yang Jing, Chen Dong
BACKGROUND: Although recent studies have indicated that gut microbiome dysbiosis was significantly associated with the onset of type 2 diabetes mellitus (T2DM), information on the role of blood microbiome in T2DM development is scarce. METHODS: Fifty incident T2DM cases and 100 matched non-T2DM controls were selected from a prospective cohort study of "135." The composition of the blood microbiome was characterized using bacterial 16S ribosomal RNA (16S rRNA) gene sequencing from pre-diagnostic blood sample...
February 4, 2019: Journal of Clinical Laboratory Analysis
Liyin Qiu, Mian Pan, Ronglian Zhang, Kunhai Ren
BACKGROUND: Histological chorioamnionitis (HCA) is one of the leading causes of spontaneous preterm birth, thus, to identify novel biomarkers for the early diagnosis of HCA is in a great need. OBJECTIVE: To investigate the diagnostic value of maternal peripheral blood platelet-to-white blood cell ratio (PLT/WBC) and platelet (PLT) counts in HCA-related preterm birth. METHODS: A total of 400 patients with preterm birth were enrolled in this study: non-HCA group (n = 193) and HCA group (n = 207), and 87 full-term pregnancies were enrolled as the control...
February 4, 2019: Journal of Clinical Laboratory Analysis
Fatma Aydın, Nilgün Çakar, Zeynep Birsin Özçakar, Nermin Uncu, Özge Başaran, Semanur Özdel, Elif Çelikel, Atilla H Elhan, Fatoş Yalçınkaya
BACKGROUND: Familial Mediterranean fever (FMF) is the most common hereditary monogenic autoinflammatory disease caused by mutations in the MEFV gene. It is controversial whether E148Q alteration is an insignificant variant or a disease-causing mutation. The aim of this study was to evaluate the clinical features and disease severity of FMF patients carrying E148Q mutation. METHODS: Files of FMF patients were retrospectively evaluated. Patients with at least one E148Q mutation were included to the study...
February 4, 2019: Journal of Clinical Laboratory Analysis
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