Journals Journal of Clinical Laboratory...

Journal of Clinical Laboratory Analysis
Kadra Mohamed Abdillahi, Fatma Ceyla Eraldemir, Irfan Kösesoy
OBJECTIVE: In response to the nuanced glycemic challenges faced by women with iron deficiency anemia (IDA) associated with diabetes, this study uses advanced machine learning algorithms to redefine hemoglobin (Hb)A1c measurement values. We aimed to improve the accuracy of glycemic interpretation by recognizing the critical interaction between erythrocytes, iron, and glycemic levels in this specific demographic group. METHODS: This retrospective observational study included 17,526 adult women with HbA1c levels recorded from 2017 to 2022...
July 10, 2024: Journal of Clinical Laboratory Analysis
Mahdi Majidpour, Ramin Saravani, Saman Sargazi, Sara Sargazi, Mahdiyeh Harati-Sadegh, Shadi Khorrami, Mohammad Sarhadi, Ali Alidadi
BACKGROUND: The importance of long noncoding RNAs (lncRNAs) in various biological processes has been increasingly recognized in recent years. This study investigated how gene polymorphism in HOX transcript antisense RNA (HOTAIR) lncRNA affects the predisposition to chronic kidney disease (CKD). METHODS: This study comprised 150 patients with CKD and 150 healthy controls. A PCR-RFLP and ARMS-PCR techniques were used for genotyping the five target polymorphisms. RESULTS: According to our findings, rs4759314 confers strong protection against CKD in allelic, dominant, and codominant heterozygote genetic patterns...
July 3, 2024: Journal of Clinical Laboratory Analysis
Gizem Zorlu Görgülügil, Ayşegül Uğur Kurtoğlu, Sevcan Uğur, Erdal Kurtoğlu
BACKGROUND/OBJECTIVES: CD71+ erythroid cells (CECs) are immature red blood cells (proerythroblasts, erythroblasts, and reticulocytes). CECs play an important role in the development of sepsis and cancer by causing immunosuppression. We examined the CEC levels in the peripheral blood of beta thalassemia (βThal) patients and investigated the relationship between CECs and the clinical status of the patients, especially splenectomy. METHODS: Sixty-eight patients with βThal (46 splenectomized and 22 nonsplenectomized) and 15 healthy controls were included in this study...
June 26, 2024: Journal of Clinical Laboratory Analysis
Zahra Miri Karam, Atieh Karimi Gohari, Mohammad Javad Rezazadeh Khabaz, Abolfazl Yari, Seyed Mahdi Emami Meybodi, Rezvan Attari, Maryam Torabi, Farzane Vafaeie, Fateme Moradi Moraddahande, Sara Amiri, Kolsoum Saeidi
BACKGROUND: Nephronophthisis-4 (NPHP4) is an inherited renal ciliopathy described by renal fibrosis and progressive impairment of kidney function. This study aimed to investigate the genetic basis and clinical manifestations of NPHP4 in two Iranian siblings. METHODS: The proband was a 27-year-old male with features of end-stage renal disease, including anemia, uremia, polyuria, and polydipsia. It is worth mentioning that he has a 22-year-old sister with a similar presentation...
June 19, 2024: Journal of Clinical Laboratory Analysis
Yuanyuan Zhang, Haiming Gao, Lu Zhang, Yunjing Zhao, Chuang Qiu, Xiaoliang Liu
INTRODUCTION: Piebaldism is a rare autosomal dominant disorder characterized by congenital white forelock and depigmented patches, which is most commonly caused by deleterious variants in the KIT gene. METHODS: Four KIT variants were identified in a piebaldism case series by whole-exome sequencing. Functional experiments, including in vitro minigene reporter assay and enzyme-linked immunosorbent assay, were carried out to elucidate the pathogenicity of the variants...
June 17, 2024: Journal of Clinical Laboratory Analysis
Hasan Ejaz, Muhammad Usman Qamar, Aisha Farhana, Sonia Younas, Alia Batool, Durreshahwar Lone, Muhammad Atif, Mashael W Alruways, Muharib Alruwaili, Ismail Hamad, Samy Selim, Bi Bi Zainab Mazhari, Ali Farooq, Kashaf Junaid
BACKGROUND: The global spread of extended-spectrum beta-lactamase (ESBL)-producing and carbapenem-resistant Enterobacterales (CRE) poses a significant concern. Acquisition of antimicrobial resistance genes leads to resistance against several antibiotics, limiting treatment options. We aimed to study ESBL-producing and CRE transmission in clinical settings. METHODS: From clinical samples, 227 ESBL-producing and CRE isolates were obtained. The isolates were cultured on bacterial media and confirmed by VITEK 2...
June 17, 2024: Journal of Clinical Laboratory Analysis
Atieh Darbandi, Milad Abdi, Shirin Dashtbin, Sajad Yaghoubi, Mohammad Sholeh, Ebrahim Kouhsari, Talieh Darbandi, Roya Ghanavati, Behrouz Taheri
INTRODUCTION: Antibodies are significant agents in the immune system and have proven to be effective in treating bacterial infections. With the advancement of antibody engineering in recent decades, antibody therapy has evolved widely. AIM: This review aimed to investigate a new method as a therapeutic platform for the treatment of bacterial infections and explore the novel features of this method in conferring pathogen specificity to broad-spectrum antibiotics...
June 13, 2024: Journal of Clinical Laboratory Analysis
Yibing Hu, Jin Ding, Yanping Chen, Qunying Wang, Xiaoyun Yang, Hongjun Hua, Xiaohua Ye
BACKGROUND: Severe acute pancreatitis (SAP) is associated with tremendous systemic inflammation, T-helper 17 (Th17) cells, and regulatory T (Treg) cells play an essential role in the inflammatory responses. Meanwhile, soluble fibrinogen-like protein 2 (Sfgl2) is a critical immunosuppressive effector cytokine of Treg cells and modulates immune responses. However, the impact of SAP induction on Sfgl2 expression and the role of Sfgl2 in immunomodulation under SAP conditions are largely unknown...
June 9, 2024: Journal of Clinical Laboratory Analysis
Qiang Ye, Qiang Zhong, Guoping Huang, Wen Zhang
OBJECTIVE: To establish the reference range of serum concentration of vitamin A (VA) and vitamin E (VE) in Southern Sichuan area of China. METHODS: From August 1, 2021, to May 31, 2023, 9482 blood tablets were received for the screening of VA and VE. The information was divided into four different age groups: ≤1 year old, 1< to ≤6 years, 6< to ≤17 years, and 17< to ≤59 years. In each age group, the four seasons were further subdivided into spring, summer, autumn, and winter, as well as male and female genders...
June 7, 2024: Journal of Clinical Laboratory Analysis
Xiaohua Xu, Yongzhi Xu, Shengqiang Liang
BACKGROUND: The interference can be a significant source of laboratory errors with the potential to cause immunoassay results to drift. Therefore, we evaluated the interference in various endogenous and exogenous substances on immunoassay for angiotensin I (Ang I), angiotensin II (Ang II), aldosterone, and renin in vitro. METHODS: Ten endogenous and eight exogenous substances were evaluated at supraphysiologic or supratherapeutic plasma levels using the screening study to identify potential interfering substances...
June 1, 2024: Journal of Clinical Laboratory Analysis
Tae Yeul Kim, Minhee Kang, Hyang Jin Shim, On-Kyun Kang, Hee Jae Huh, Nam Yong Lee
BACKGROUND: Rapid antimicrobial susceptibility testing (AST) for bloodstream infections (BSIs) facilitates the optimization of antimicrobial therapy, preventing antimicrobial resistance and improving patient outcomes. QMAC-dRAST (QuantaMatrix Inc., Korea) is a rapid AST platform based on microfluidic chip technology that performs AST directly using positive blood culture broth (PBCB). This study evaluated the performance of QMAC-dRAST for Gram-negative bacteria using PBCB and subcultured colony isolates, comparing it with that of VITEK 2 (bioMérieux, France) using broth microdilution (BMD) as the reference method...
May 28, 2024: Journal of Clinical Laboratory Analysis
Maryam Salimi, Javad Javidnia, Leila Faeli, Azam Moslemi, Mohammad Taghi Hedayati, Iman Haghani, Seyed Reza Aghili, Maryam Moazeni, Parisa Badiee, Maryam Roudbari, Hossein Zarrinfar, Rasoul Mohammadi, Ensieh Lotfali, Sadegh Nouripour-Sisakht, Seyedmojtaba Seyedmousavi, Tahereh Shokohi, Mahdi Abastabar
BACKGROUND: The current study aimed to identify Iranian Nakaseomyces (Candida) glabrata complex species in the clinical isolates and determine their antifungal susceptibility profile. METHODS: In total, 320 N. glabrata clinical isolates were collected from patients hospitalized in different geographical regions of Iran. The initial screening was performed by morphological characteristics on CHROMagar Candida. Each isolate was identified by targeting the D1/D2 rDNA using a multiplex-PCR method...
May 22, 2024: Journal of Clinical Laboratory Analysis
Caner Turan, Ali Yurtseven, Pinar Yazici Ozkaya, Elif Azarsiz, Eylem Ulas Saz
BACKGROUND: Despite advancements in antibiotic therapy and resuscitation protocols, sepsis and septic shock remain major contributors to morbidity and mortality in children. We aimed to investigate the utility of soluble urokinase plasminogen activator receptor (suPAR) for the early detection of septic shock and to evaluate its accuracy in predicting mortality. METHODS: A prospective study was conducted in a tertiary pediatric emergency department (ED), enrolling patients diagnosed with the sepsis, severe sepsis, or septic shock...
May 6, 2024: Journal of Clinical Laboratory Analysis
Ayşegül Uğur Kurtoğlu, Sevcan Uğur, Mesut Göçer, Erdal Kurtoğlu
AIM: In this study, we investigated how splenectomy affects natural killer (NK) cell levels in patients with β-thalassemia major (β-TM). MATERIALS AND METHODS: Seventy patients with β-TM (38 splenectomized and 32 nonsplenectomized) and 25 healthy controls were included in this study. The hemogram parameters, ferritin, T lymphocyte, T-helper cell, T-suppressor cell, and NK cell numbers, were measured. RESULTS: The T lymphocyte (CD3+ ) level was found to be significantly higher in the patient group (p < 0...
May 2024: Journal of Clinical Laboratory Analysis
Itahisa Hernández-Chico, Enrique Rodríguez-Guerrero, Manuela Expósito-Ruiz, José María Navarro-Marí, José Gutiérrez-Fernández
BACKGROUND: Infections by glucose-nonfermenting gram-negative bacilli (NFGNB) pose a major public health problem due to multiresistance to beta-lactam antibiotics, especially plasmid-borne carbapenemases. Their detection by microbiology laboratories is challenging, and there is a need for easy-to-use and reliable diagnostic techniques. Our objective was to evaluate an in-house screening method to presumptively detect carbapenemases in NFGNB in a simple and clinically useful manner. METHODS: The study included 175 NFGNB isolates from urinary, respiratory, and rectal samples...
April 15, 2024: Journal of Clinical Laboratory Analysis
Sophie Brunner-Ziegler, Bernd Jilma, Gabriele Grimm, Petra Jilma-Stohlawetz
BACKGROUND: In newborns, elevated nucleated red blood cell (NRBC) levels can be associated with enhanced erythropoietic stress and might be predictive for adverse outcome. Also, the presence of NRBC in peripheral blood might lead to erroneous enumeration results of white blood cells in automated hematology analyzers. We aimed to assess the comparability of the Sysmex XN 1000 to manual slide reviews and correlation of NRBC with inflammation markers. METHODS: Specimens of 3397 children under 1 year were compared by automated and microscopic NRBC enumeration...
April 15, 2024: Journal of Clinical Laboratory Analysis
Filipa P Freitas, Jorge Reis, Joana Oliveira, Pedro Mota Veiga, Ana Raquel Paiva, Rui Soares
BACKGROUND: The Beckman Coulter DxH 900 is a haematological analyser capable of counting and sizing blood cells, and obtaining a complete blood cell count (CBC). This analyses different parameters of red blood cells (RBC), platelets and white blood cells/leukocytes. Some automated CBC counters present limitations due to specimen characteristics, abnormal cells or both factors. In the presence of abnormalities, the DxH 900 has a flagging system, warning the laboratory technician that something needs to be verified...
April 9, 2024: Journal of Clinical Laboratory Analysis
Shao-Wei Hua, Jie Wang, Zi-Jin Zhao, Feng-Yu Tian, Meng Zhao, Yu-Xin Wang, Rui-Qing Zhang, Zhi-Qiang Han, Shi-Jue Gao, Xiao-Na Lv, Hong-Yi Li, Xin-Xin Shen, Xue-Jun Ma, Zhi-Shan Feng
OBJECTIVE: This study aimed to establish a highly sensitive and rapid single-tube, two-stage, multiplex recombinase-aided qPCR (mRAP) assay to specifically detect the khe, blaKPC-2 , and blaNDM-1 genes in Klebsiella pneumoniae. METHODS: mRAP was carried out in a qPCR instrument within 1 h. The analytical sensitivities of mRAP for khe, blaKPC-2 , and blaNDM-1 genes were tested using recombinant plasmids and dilutions of reference strains. A total of 137 clinical isolates and 86 sputum samples were used to validate the clinical performance of mRAP...
April 8, 2024: Journal of Clinical Laboratory Analysis
Yan Qin, Yuhan Jia, Congcong Liang, Rui Fu, Zhaojun Liang, Yanlin Wang, Min Feng, Chong Gao, Jing Luo
BACKGROUND: Detection of IgG subclasses (IgGSc) is vital for the diagnosis and management of disease, especially IgG4-related diseases (IgG4-RD). This study aimed to evaluate the performances of the chemiluminescent immunoassay (CLIA) for detecting IgGSc and diagnosing IgG4-RD by IgGSc. METHODS: A total of 40 individuals with IgG4-RD, 40 with primary Sjogren's syndrome (pSS), and 40 healthy controls (HCs) were enrolled. Serum samples were collected for the simultaneous detection of IgG1, IgG2, IgG3, and IgG4 by the Siemens immunonephelometric assay and the CLIA...
April 2, 2024: Journal of Clinical Laboratory Analysis
Chunping Gu, Yongfang Zhang, Fumiao Yuan, Kaibin Huang, Zhenzhou Lin, Qiong Chen, Yan Chen, Yongming Wu, Dongmei Wang, Shengnan Wang
OBJECTIVE: This study aimed to indicate whether a declined plasma concentration of valproic acid (VPA) induced by co-administration of meropenem (MEPM) could affect the antiepileptic efficacy of VPA. METHODS: We retrospectively reviewed data of hospitalized patients who were diagnosed with status epilepticus or epilepsy between 2010 and 2019. Patients co-administered VPA and MEPM during hospitalization were screened and assigned to the exposure group, while those co-administerd VPA and other broad-spectrum antibiotics were allocated to the control group...
April 2, 2024: Journal of Clinical Laboratory Analysis
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