Afsaneh Mohammadnejad, Mette Soerensen, Jonas Mengel-From, Marianne Nygaard, Maria Timofeeva, Liang He, Signe Bedsted Clemmensen, Ulrich Halekoh, Rikke Hedegaard Dahlrot, Qihua Tan, Jacob B Hjelmborg
Identifying genetic factors affecting the regulation of the O-6-Methylguanine-DNA Methyltransferase (MGMT) gene and estimating the genetic contribution of the MGMT gene through within-pair correlation in monozygotic twin pairs is of particular importance in various types of cancer such as glioblastoma. We used gene expression data in whole blood from 448 monozygotic twins from the Middle Age Danish Twins (MADT) study to investigate genetic regulation of the MGMT gene by performing a genome-wide association study (GWAS) of the variation in MGMT expression...
March 20, 2023: Genomics
Jun-Song Ren, Wen Bai, Jing-Juan Ding, Ya Zhao, Su-Yu Wang, Xi Chen, Qin Jiang
Wet age-related macular degeneration (wAMD) is the leading cause of blindness among the elderly in industrialized nations. Anti-vascular epidermal growth factor (VEGF) therapy via intravitreal injection is the most effective clinical treatment for wAMD due to high concentrations of VEGF that promote choroidal neovascularization. While PIWI proteins participate in various biological processes, their function in AMD remains unclear. In this study, we discovered that PIWIL4 expression is elevated in a laser-induced choroidal neovascularization (CNV) model and that it regulates angiogenesis in vitro and in vivo...
March 17, 2023: Genomics
Xuezheng Xu, Yikun Ju, Xueheng Zhao, Pu Yang, Fang Zhu, Bairong Fang
Skin cutaneous melanoma (SKCM) is the most life-threatening skin cancer and lacks early detection and effective treatment strategies. Many long noncoding RNAs are associated with the development of tumors and may serve as potential immunotherapeutic targets. In this study, microarray analysis was performed to screen for differentially expressed lncRNAs between SKCM and normal tissues, and SMG7-AS1 was identified as an upregulated lncRNA in SKCM. Subsequently, bioinformatic analysis revealed that dysregulation of SMG7-AS1 influences metastasis and immune infiltration...
March 15, 2023: Genomics
Linyao Zhao, Congkuan Song, Yong Li, Fanen Yuan, Qingyu Zhao, Huimin Dong, Baohui Liu
BACKGROUND: Glioma is the most common primary tumor in the human central nervous system. This study was designed to explore the expression of BZW1 in glioma and its relevance to the clinicopathological features and outcome of glioma patients. METHODS: Glioma transcription profiling data were obtained from The Cancer Genome Atlas (TCGA). TIMER2, GEPIA2, GeneMANIA, and Metascape were searched in the present study. Cell and animal experiments were conducted to verify the effect of BZW1 on glioma cell migration in vitro and in vivo...
March 10, 2023: Genomics
Golam Rbbani, Artem Nedoluzhko, Prabhugouda Siriyappagouder, Fedor Sharko, Jorge Galindo-Villegas, Joost A M Raeymaekers, Rajesh Joshi, Jorge M O Fernandes
Muscle growth in teleosts is a complex biological process orchestrated by numerous protein-coding genes and non-coding RNAs. A few recent studies suggest that circRNAs are involved in teleost myogenesis, but the molecular networks involved remain poorly understood. In this study, an integrative omics approach was used to determine myogenic circRNAs in Nile tilapia by quantifying and comparing the expression profile of mRNAs, miRNAs, and circRNAs in fast muscle from full-sib fish with distinct growth rates. There were 1947 mRNAs, 9 miRNAs, and 4 circRNAs differentially expressed between fast- and slow-growing individuals...
March 9, 2023: Genomics
Christopher J Giacoletto, Ronald Benjamin, Hong-Wen Deng, Jerome I Rotter, Martin R Schiller
The genetic code has degenerate codons that produce no change in the translated protein sequence and are generally thought to be silent. However, some synonymous variants are clearly not silent. Herein, we questioned the frequency of non-silent synonymous variants. We tested how random synonymous variants in the HIV Tat transcription factor effect transcription of an LTR-GFP reporter. Our model system has the advantage of directly measuring the function of the gene in human cells. Approximately, 67% of synonymous variants in Tat were non-silent, either having reduced activity or were full loss-of-function alleles...
March 7, 2023: Genomics
Zachary Johnson, Xiguang Xu, Yu Lin, Hehuang Xie
Post-transcriptional RNA modifications have been recognized as key regulators of neuronal differentiation and synapse development in the mammalian brain. While distinct sets of 5-methylcytosine (m5 C) modified mRNAs have been detected in neuronal cells and brain tissues, no study has been performed to characterize methylated mRNA profiles in the developing brain. Here, together with regular RNA-seq, we performed transcriptome-wide bisulfite sequencing to compare RNA cytosine methylation patterns in neural stem cells (NSCs), cortical neuronal cultures, and brain tissues at three postnatal stages...
March 6, 2023: Genomics
Juliana González-Tobón, Allison Diaz, Tyler C Helmann, Mary Ann Karp, Margery Daughtrey, Paul V Stodghill, Melanie J Filiatrault
The taxonomy of Pseudomonas has been extensively studied, yet the determination of species is currently difficult because of recent taxonomic changes and the lack of complete genomic sequence data. We isolated a bacterium causing a leaf spot disease on hibiscus (Hibiscus rosa-sinensis). Whole genome sequencing revealed similarity to Pseudomonas amygdali pv. tabaci and pv. lachrymans. The genome of this isolate (referred to as P. amygdali 35-1) shared 4987 genes with P. amygdali pv. hibisci, but possessed 204 unique genes and contained gene clusters encoding putative secondary metabolites and copper resistance determinants...
March 6, 2023: Genomics
Guo Yang, Ting Li, Jiayu Liu, Zhen Quan, Miao Liu, Yuan Guo, Yingying Wu, Liping Ou, Xiaohou Wu, Yongbo Zheng
Prostate cancer (PCa) is a common malignant cancer in elderly males in Western countries. Whole-genome sequencing confirmed that long non-coding RNAs (lncRNAs) are frequently altered in castration-resistant prostate cancer (CRPC) and promote drug resistance to cancer therapy. Therefore, elucidating the prospective role of lncRNAs in PCa oncogenesis and progression is of remarkable clinical significance. In this study, gene expression in prostate tissues was determined using RNA-sequencing datasets, and the gene diagnostic and prognostic values of CRPC were analyzed using bioinformatics...
March 6, 2023: Genomics
Li Guowei, Lu Xiufang, Xu Qianqian, Jin Yanping
To explore the regulatory mechanism of FDX1 methylation in malignant phenotype of glioma. We screened pathway through bioinformatic analysis, proceeded RIP and cell models to verify the regulation of RNAs and mitophagy. We chose Clone and Transwell assay to evaluate the malignant phenotype of glioma cells. MMP was detected by flow cytometry and mitochondrial morphology was observed by TEM. We constructed animal models to study the sensibility of glioma cells to cuproptosis. We successfully obtained the signal pathway...
March 6, 2023: Genomics
Linan Bao, Yang Chu, Hui Kang
Liver fibrosis is the result of the accumulation of extracellular matrix (ECM) that cannot be cleared. Bioinformatic analysis showed that LINC01711 was significantly overexpressed in hepatic fibrosis. The regulatory mechanism of LINC01711 was clarified and confirmed the transcription factors associated with LINC01711. Functionally, LINC01711 promoted LX-2 cell proliferation and migration, indicating that it exerts effects promoting the progression of hepatic fibrosis. Mechanistically, LINC01711 increased the expression of xylosyltransferase 1 (XYLT1), which is an important protein for constructing the ECM...
March 3, 2023: Genomics
Xu Cai, Shaihong Zhu
The role of VDAC1 in osteosarcoma is unclear. We explored the effect of VDAC1 on osteosarcoma development by combining bioinformatic analysis and experimental identification. This study suggested that VDAC1 is an independent prognostic factor for osteosarcoma. Patients with high VDAC1 expression have a poor survival rate. VDAC1 was overexpressed in osteosarcoma cells. After silencing VDAC1, the proliferation of osteosarcoma cells decreased, and the apoptosis rate increased. Gene set variation analysis and gene set enrichment analysis indicated that VDAC1 was associated with the MAPK signaling pathway...
March 3, 2023: Genomics
Ping Wang, Yanli Zhang, Xiaodong Lv, Jian Zhou, Shundong Cang, Yongping Song
We sought to extend our observation of LncRNA ADAMTS9-AS1 and to specifically uncover its role on the stemness of lung adenocarcinoma (LUAD) cancer cells. ADAMTS9-AS1 was poorly expressed in LUAD. The high ADAMTS9-AS1 expression was positively associated with overall survival. ADAMTS9-AS1 overexpression attenuated the colony-forming capacity and reduced stem cell-like population of LUAD cancer stem cells (CSCs). Furthermore, ADAMTS9-AS1 overexpression increased E-cadherin expression in addition to the downregulated expressions of Fibronectin and Vimentin in LUAD spheres...
March 2, 2023: Genomics
Shun Ke, Jing Wang, Jun Lu, Minghao Fang, Ruichao Li
OBJECTIVES: We aimed at probing impact of LINC00858 on esophageal squamous cell carcinoma (ESCC) progression via ZNF184-FTO-m6 A-MYC axis. METHODS: Expression of related genes (LINC00858, ZNF184, FTO, and MYC) was detected in ESCC tissues or cells and their relationships were assessed. After expression alterations in ESCC cells, cell proliferation, invasion, migration, and apoptosis were detected. Tumor formation in nude mice was conducted. RESULTS: LINC00858, ZNF184, FTO, and MYC were overexpressed in ESCC tissues and cells...
March 1, 2023: Genomics
Xi Zeng, Ning Wang, Chuanying Xiang, Qiang Liu, Daiyu Li, Yangyang Zhou, Xiaomin Zhang, Yu Xie, Weijun Zhang, Hong Yang, Ming Jiang, Xianchun Zong, Quanming Zou, Yun Shi
The role of peptidoglycan-associated lipoprotein (Pal) in A. baumannii pathogenesis remains unclear. Here, we illustrated its role by constructing a pal deficient A. baumannii mutant and its complementary strain.Transcriptome analysis of the WT and pal mutant by revealed a total of 596 differentially expressed genes. Gene Ontology analysis revealed that pal deficiency caused the downregulation of genes related to material transport and metabolic processes. The pal mutant showed a slower growth and was sensitive to detergent and serum killing compared to WT strain, whereas, the complemented pal mutant showed rescued phenotype...
March 1, 2023: Genomics
Ziyu Wang, Fenglian Xu, Xueyan Zhao, Yi Zhang, Xi Wang, Zhirui Zhang, Lele Zixin Yang, Joshua Sam Badshah, Baohui Xu, Rong Xie, Weirong Fang
Astrocytes activate and crosstalk with neurons influencing inflammatory responses following ischemic stroke. The distribution, abundance, and activity of microRNAs in astrocytes-derived exosomes after ischemic stroke remains largely unknown. In this study, exosomes were extracted from primary cultured mouse astrocytes via ultracentrifugation, and exposed to oxygen glucose deprivation/re‑oxygenation injury to mimic experimental ischemic stroke. SmallRNAs from astrocyte-derived exosomes were sequenced, and differentially expressed microRNAs were randomly selected and verified by stem-loop real time quantitative polymerase chain reaction...
February 28, 2023: Genomics
Jihong Zhang, Quanwu Liang, Yuqiong Li, Zhiping Deng, Ge Song, Haihua Wang, Mingli Yan, Xuewen Wang
Tomato is a widely cultivated fruit and vegetable and is valued for its flavor, colour, and nutritional value. C6 -aldehydes, such as (E)-2-hexenal, not only have antibacterial and antifungal properties but also function as signaling molecules that control the defense mechanisms of plants, including tomatoes. In this study, we used liquid chromatography-mass spectrometry (LC-MS) and RNA sequencing techniques to generate metabolome and transcriptome datasets that elucidate the molecular mechanisms regulating defense responses in tomato leaves exposed to (E)-2-hexenal...
February 26, 2023: Genomics
R Ros-Freixedes, R N Pena, C Richart, J Nadal
The genetic dynamics of wild populations with releases of farm-reared reinforcements are very complex. These releases can endanger wild populations through genetic swamping or by displacing them. We assess whether there are genomic differences between wild and farm-reared red-legged partridges (Alectoris rufa) and to identify differential selection signals between both populations. We sequenced the whole genome of 30 wild and 30 farm-reared partridges. Both partridges had similar nucleotide diversity (π)...
February 25, 2023: Genomics
Rayner González-Prendes, Martijn F L Derks, M A M Groenen, Raquel Quintanilla, Marcel Amills
In general, the relationship between the predicted functional consequences of missense mutations mapping to genes known to be involved in human diseases and the severity of disease manifestations is weak. In this study, we tested in pigs whether missense single nucleotide polymorphisms (SNPs), predicted to have consequences on the function of genes related to lipid metabolism are associated with lipid phenotypes. Association analysis demonstrated that nine out of 72 nominally associated SNPs were classified as "highly" or "very highly consistent" in silico-predicted functional mutations and did not show association with lipid traits expected to be affected by inactivation of the corresponding gene...
February 24, 2023: Genomics
Forough Joudaki, Ahmad Ismaili, Seyed Sajad Sohrabi, Seyedeh Zahra Hosseini, Danyal Kahrizi, Hadi Ahmadi
Gall oak (Quercus infectoria) is a native tree of Iran, whose gall extract is used to treat many diseases. The presence of abundant secondary metabolites with various bioactivities in this plant has made it medically important. Despite its medicinal value, due to the lack of genomic information, the biosynthetic pathways of these compounds in this species are still unknown. The current research was aimed at observing, characterizing, and investigating the biosynthetic pathways of these compounds in Q.infectoria...
February 23, 2023: Genomics
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