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Journals Toxicology in Vitro : An Inter...

Toxicology in Vitro : An International Journal Published in Association with BIBRA

https://read.qxmd.com/read/39004236/modulation-of-adipogenesis-and-lipogenesis-by-indomethacin-and-pantoprazole
#1
JOURNAL ARTICLE
Bita Entezari, Hasan Akbaba, Hande Gurer-Orhan
Endocrine disruptors are suggested to act as potential "obesogens" by interacting with various metabolic processes in adipose tissue. Besides industrial chemicals that are blamed to act as endocrine disruptors as well as obesogens, pharmaceuticals can also cause obesogenic effects as unintended adverse effects. However, limited studies evaluated the obesogenic adverse effects of pharmaceuticals. Based on this information, the present study aimed to investigate possible in vitro adipogenic/lipogenic potential of indomethacin and pantoprazole that are prescribed during pregnancy...
July 12, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/39002813/integrated-transcriptomic-and-metabolomic-analysis-reveals-the-underlying-mechanisms-for-male-reproductive-toxicity-of-polystyrene-nanoplastics-in-mouse-spermatocyte-derived-gc-2spd-ts-cells
#2
JOURNAL ARTICLE
Hang Han, Zhen Zhang, Bo Xu, Liyang Ding, Hong Yang, Tiantian He, Xing Du, Xiuying Pei, Xufeng Fu
BACKGROUND: Polystyrene nanoplastics (PS-NPs), are ubiquitous pollution sources in human environments, posing significant biosafety and health risks. While recent studies, including our own, have illustrated that PS-NPs can breach the blood-testis barrier and impact germ cells, there remains a gap in understanding their effects on specific spermatogenic cells such as spermatocytes. METHODS AND RESULTS: Herein, we employed an integrated approach encompassing phenotype, metabolomics, and transcriptomics analyses to assess the molecular impact of PS-NPs on mouse spermatocyte-derived GC-2spd(ts) cells...
July 11, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38996828/qsar-modeling-for-cytotoxicity-of-sulfur-containing-shikonin-oxime-derivatives-targeting-hct-15-mgc-803-bel-7402-and-mcf-7-cell-lines
#3
JOURNAL ARTICLE
Abderrahim Diane, Salima Ben Tahar, Abdennacer El Mrabet, Reda Rabie, Taoufiq Saffaj, Bouchaib Ihssane
Targeting cancer cells through drug-based treatment or combination therapy protocols involving chemical compounds can be challenging due to multiple factors, including their resistance to bioactive compounds and the potential of drugs to damage healthy cells. This study aims to investigate the relationship between the structure of novel sulfur-containing shikonin oxime compounds and the corresponding cytotoxicity against four cancer types, namely colon, gastric, liver, and breast cancers, through computational chemistry tools...
July 10, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38996827/the-recovery-from-taxane-mediated-apoptosis-in-pc-3-castration-resistant-metastatic-prostate-cancer-cells
#4
JOURNAL ARTICLE
Gamze Guney Eskiler, Asuman Deveci Ozkan, Merve Acikel Elmas, Melek Ozturk, Serap Arbak
Here, we revealed the reversibility of cabazitaxel (CBZ)-induced apoptosis in PC-3 castration resistant metastatic prostate cancer cells (mCRPC) through the hallmarks of apoptosis. The recovery of PC-3 cells from apoptosis upon removal of CBZ at different recovery periods was evaluated by Annexin V, DNA damage, oxidative damage, mitochondrial membrane depolarization, and caspase activation. Our results showed that the administration of CBZ caused apoptosis for 72 h in PC-3 cells. However, recovered cells exhibited decreased nuclear damage, plasma membrane disruption, ROS level, release cytochrome c level and caspase-3 activation with upregulation of Bcl-2 expression upon removal of especially 1 nM CBZ for 24 h recovery period in PC-3 cells...
July 10, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38972516/evaluating-the-combined-and-individual-cytotoxic-effect-of-beauvericin-enniatin-b-and-ochratoxin-a-on-breast-cancer-cells-leukemia-cells-and-fresh-peripheral-blood-mononuclear-cells
#5
JOURNAL ARTICLE
Ana Juan-García, Ana-María Ilie, Cristina Juan, Lola Martínez
Beauvericin (BEA), Enniatin B (ENN B), and Ochratoxin A (OTA) are mycotoxins produced by fungi species. Their main effect on several organs and systems is associated with chronic exposure going from immunotoxicity, estrogenic disorders, and renal failure to cancer (in animals and humans). OTA belongs to Group 1 according to the International Agency for Research in Cancer (IARC) and it has legislated limited values; not happening for BEA nor ENN B. Exposure to mixtures of mycotoxins occurs through food intake in daily consumption...
July 5, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38972515/toxicity-of-new-psychoactive-substance-threo-4-methylmethylphenidate-4-mmph-prediction-of-toxicity-using-in-silico-methods
#6
JOURNAL ARTICLE
Łukasz Niżnik, Karolina Jabłońska, Michał Orczyk, Martyna Orzechowska, Joanna Toporowska-Kaźmierak, Marta Sowińska, Judyta Jasińska, Kamil Jurowski
This study represents the first application of in silico methods to evaluate the toxicity of 4-methylphenidate (4-Mmph), a new psychoactive substance (NPS). Using advanced in silico toxicology tools, it was feasible to anticipate key aspects of 4-Mmph's toxicological profile, including acute toxicity (LD50 ), genotoxicity, cardiotoxicity, and possible endocrine disruption. The findings indicate significant acute toxicity with variability among species, a high potential for adverse effects in the gastrointestinal system and lungs, a low genotoxic potential, a significant likelihood of skin irritation, and a notable cardiotoxicity risk associated with hERG channel inhibition...
July 5, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38972514/predicting-the-binding-affinity-of-chemicals-to-estrogen-receptor-using-hansen-solubility-parameters
#7
JOURNAL ARTICLE
Nobuyuki Fujiwara, Len Ito
Receptor-binding tests for the receptors of various substances are widely employed to identify drug candidates and predict the biological effects of chemical substances. Here, the results of chemicals binding to estrogen receptor (ER) reported in a validation study of the Organization for Economic Cooperation and Development TG 455 and the Hansen solubility parameter (HSP) values of the test substances were compared and examined using the Hansen sphere method, thus predicting potential HSPs that correspond to the ER-binding domain of agonists...
July 5, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38971396/aerosol-exposure-at-air-liquid-interface-ae-ali-in-vitro-toxicity-system-characterisation-particle-deposition-and-the-importance-of-air-control-responses
#8
JOURNAL ARTICLE
Alison Buckley, Chang Guo, Adam Laycock, Xianjin Cui, Marie-France Belinga-Desaunay-Nault, Eugenia Valsami-Jones, Martin Leonard, Rachel Smith
Experimental systems allowing aerosol exposure (AE) of cell cultures at the air-liquid-interface (ALI) are increasingly being used to assess the toxicity of inhaled contaminants as they are more biomimetic than standard methods using submerged cultures, however, they require detailed characterisation before use. An AE-ALI system combining aerosol generation with a CULTEX® exposure chamber was characterised with respect to particle deposition and the cellular effects of filtered air (typical control) exposures...
July 4, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38950639/selected-phytocannabinoids-inhibit-sn-38-and-cytokine-evoked-increases-in-epithelial-permeability-and-improve-intestinal-barrier-function-in-vitro
#9
JOURNAL ARTICLE
Dylan T Marsh, Scott D Smid
Irinotecan use is linked to the development of gastrointestinal toxicity and inflammation, or gastrointestinal mucositis. Selected phytocannabinoids have been ascribed anti-inflammatory effects in models of gastrointestinal inflammation, associated with maintaining epithelial barrier function. We characterised the mucoprotective capacity of the phytocannabinoids: cannabidiol, cannabigerol, cannabichromene and cannabidivarin in a cell-based model of intestinal epithelial stress occurring in mucositis. Transepithelial electrical resistance (TEER) was measured to determine changes in epithelial permeability in the presence of SN-38 (5 μM) or the pro-inflammatory cytokines TNFα and IL-1β (each at 100 ng/mL), alone or with concomitant treatment with each of the phytocannabinoids (1 μM)...
June 29, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38945378/circpdss1-hsa_circ_0017998-silencing-induces-ferroptosis-in-non-small-cell-lung-cancer-cells-by-modulating-the-mir-137-slc7a11-gpx4-gclc-axis
#10
JOURNAL ARTICLE
Ling Wu, Ni Li, Linwen Zhu, Guofeng Shao
BACKGROUND: Circular RNAs (circRNAs) regulate the tumorigenesis of non-small-cell lung cancer (NSCLC). CircPDSS1 (hsa_circ_0017998) has been newly discovered, and its role in NSCLC remains elusive. We aimed to investigate the functional roles and downstream targets of circPDSS1 in NSCLC cells. MATERIALS AND METHODS: Cellular viabilities were measured through the Cell Counting Kit-8 (CCK-8) assay, whereas cell death was assessed through flow cytometry. The lactate dehydrogenase activity, malondialdehyde levels, ferrous iron, and reactive oxygen species were measured using commercial assay kits...
June 28, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38945377/human-in-vitro-percutaneous-absorption-of-bisphenol-s-assessment-of-the-skin-reservoir-and-occlusion-effects
#11
JOURNAL ARTICLE
Fabrice Marquet, Catherine Champmartin, Claire Seiwert, Matthieu Aubertin, Stéphane Viton, Lisa Chedik, Frédéric Cosnier
Bisphenol S (BPS) was introduced in many industrial and commercial applications as a presumed safer alternative to bisphenol A. However, concerns have been raised surrounding skin absorption and potential persistence of BPS and its related toxic effects in humans. A previous study revealed the likelihood of a reservoir building up in exposed skin. Here, we studied the interactions of BPS solubilized in acetone, ultrapure water, or artificial sebum with freshly excised human skin samples. In vitro tests were performed in static Franz diffusion cells, to explore reservoir and occlusion effects, absorption and metabolism...
June 28, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38945376/selective-inhibition-of-hdac6-by-n-acylhydrazone-derivative-reduces-the-proliferation-and-induces-senescence-in-carcinoma-hepatocellular-cells
#12
JOURNAL ARTICLE
Guilherme Álvaro Ferreira-Silva, Daniel Alencar Rodrigues, Carolina Girotto Pressete, Ester Siqueira Caixeta, Angel Mauricio Castro Gamero, Marta Miyazawa, João Adolfo Costa Hanemmann, Carlos Alberto Manssour Fraga, Alexandre Ferro Aissa, Marisa Ionta
Hepatocellular carcinoma (HCC) is a significant contributor to cancer-related deaths globally. Systemic therapy is the only treatment option for HCC at an advanced stage, with limited therapeutic response. In this study, we evaluated the antitumor potential of four N-acylhydrazone (NAH) derivatives, namely LASSBio-1909, 1911, 1935, and 1936, on HCC cell lines. We have previously demonstrated that the aforementioned NAH derivatives selectively inhibit histone deacetylase 6 (HDAC6) in lung cancer cells, but their effects on HCC cells have not been explored...
June 28, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38936442/three-dimensional-reconstruction-of-skin-with-melanoma-a-model-for-study-of-invasion-in-vitro
#13
JOURNAL ARTICLE
Elenn Suzany Pereira Aranha, Leilane Bentes de Sousa, Bianca Lima Sousa, Emerson Lucena da Silva, Felipe Pantoja Mesquita, Emerson Silva Lima, Ana Paula Negreiros Nunes Alves, Maria Elisabete Amaral de Moraes, Raquel Carvalho Montenegro, Marne Carvalho de Vasconcellos
Melanoma is a type of tumor skin with high metastatic potential. Reconstructed human skin, development for pre-clinic assay, are make using primary human cells, but with same limitations. The aim this study was to characterize a cell culture model, with structure similar to human skin containing melanoma cells entirely from cell lines. Reconstructed skin with melanoma were development using human fibroblasts (MRC5), human epidermal keratinocytes (HaCat), and human melanoma (SK-MEL-28) embedded in collagen type I...
June 25, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38936441/influence-of-alkylthio-and-arylthio-derivatives-of-tert-butylquinone-on-the-induction-of-dna-damage-in-a-human-hepatocellular-carcinoma-cell-line-hepg2
#14
JOURNAL ARTICLE
Djordjević Aleksić Jelena, Kolarević Stoimir, Jovanović Marić Jovana, Kračun-Kolarević Margareta, Žegura Bojana, Štern Alja, Sladić Dušan, Novaković Irena, Vuković-Gačić Branka
The aim of this study was to investigate the effects of tert-butylquinone (TBQ) and its alkylthio and arylthio derivatives on DNA in vitro, using acellular and cellular test systems. Direct interaction with DNA was studied using the plasmid pUC19. Cytotoxic (MTS assay) and genotoxic (comet assay and γH2AX focus assays) effects, and their influence on the cell cycle were studied in the HepG2 cell line. Our results show that TBQ and its derivatives did not directly interact with DNA. The strongest cytotoxic effect on the HepG2 cells was observed for the derivative 2-tert-butyl-5,6-(ethylenedithio)-1,4-benzoquinone (IC50 64...
June 25, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38906201/exploring-the-combined-impact-of-cisplatin-and-copper-cysteamine-nanoparticles-through-chemoradiation-an-in-vitro-study
#15
JOURNAL ARTICLE
Mahsa Ejtema, Nahid Chegeni, Amanollah Zarei Ahmady, Zeinab Salehnia, Masoume Shams, Sasan Razmjoo
Copper-Cysteamine nanoparticles (Cu-Cy NPs) have emerged as promising radiosensitizers in cancer treatment. This study aims to investigate the combined therapeutic effect of these nanoparticles and cisplatin using a clinical linear accelerator to enhance the efficacy of chemoradiation therapy for cervical cancer. Following successful synthesis and characterization of Cu-Cy NPs, the cytotoxicity effect of these nanoparticles and cisplatin in various concentrations was evaluated on HeLa cancer cells, individually and in combination...
June 19, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38906200/-1-h-nmr-spectroscopic-characterisation-of-hepg2-cells-as-a-model-metabolic-system-for-toxicology-studies
#16
JOURNAL ARTICLE
Maren Jinks, Emily C Davies, Berin A Boughton, Samantha Lodge, Garth L Maker
The immortalised human hepatocellular HepG2 cell line is commonly used for toxicology studies as an alternative to animal testing due to its characteristic liver-distinctive functions. However, little is known about the baseline metabolic changes within these cells upon toxin exposure. We have applied high-resolution 1 H Nuclear Magnetic Resonance (NMR) spectroscopy to characterise the biochemical composition of HepG2 cells at baseline and post-exposure to hydrogen peroxide (H2 O2 ). Metabolic profiles of live cells, cell extracts, and their spent media supernatants were obtained using 1 H high-resolution magic angle spinning (HR-MAS) NMR and 1 H NMR spectroscopic techniques...
June 19, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38901786/construction-of-an-in-vitro-simulated-one-compartment-extravascular-administration-model-and-its-comparison-with-classic-in-vitro-administration-model-in-copper-chloride-induced-hepg2-cell-death
#17
JOURNAL ARTICLE
Lailai Yan, Dawei Fu, Jie Chen, Mingmei Hao, Juanling Fu, Biyun Yao, Weidong Hao, Peng Zhao
In this study, we designed an in vitro administration device based on compartment model theory and utilized it to construct an in vitro simulated one compartment extravascular administration model of copper chloride. Within the Cmax range of 3.91-1000.00 μM, the measured concentration-time curves of the simulated one compartment extravascular administration model almost coincide with the corresponding theoretical curves. The measured values of toxicokinetic parameters, including ke , T1/2 , ka , T1/2a , Tmax , Cmax , CL, and AUC0-∞ are close to the corresponding theoretical values...
June 18, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38901785/cannabidiol-protects-mouse-hippocampal-neurons-from-neurotoxicity-induced-by-amyloid-%C3%AE-peptide-25-35
#18
JOURNAL ARTICLE
Karen Del Carmen Barboza Salgado, Rosiene Gomes de Freitas Nascimento, Pedro José Fernandes Nunes Coelho, Laser Antonio Machado Oliveira, Katiane de Oliveira Pinto Coelho Nogueira
Alzheimer's disease (AD), the most prevalent form of dementia worldwide, is a significant health concern, according to the World Health Organization (WHO). The neuropathological diagnostic criteria for AD are based on the deposition of amyloid-β peptide (Aβ) and the formation of intracellular tau protein tangles. These proteins are associated with several overlapping neurodegenerative mechanisms, including oxidative stress, mitochondrial dysfunction, lipid peroxidation, reduced neuronal viability, and cell death...
June 18, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38876227/effect-of-long-term-inorganic-arsenic-exposure-on-erythropoietin-production-in-vitro
#19
JOURNAL ARTICLE
Md Anamul Haque, Akari Yoshimoto, Hiroshi Nakagawa, Kazuhiko Nishimura
Arsenic is widely present in the environment in trivalent and pentavalent forms; long-term arsenic exposure due to environmental pollution has become a problem. Previous reports have shown that 24-h exposure to arsenate (as pentavalent arsenic) potentiates erythropoietin (EPO) production via reactive oxygen species (ROS) in EPO-producing HepG2 cells. However, the effects of long-term arsenate exposure on EPO production remain unclear. In HepG2 cells subcultured for 3 weeks in the presence of arsenate, EPO mRNA levels were lower than those in untreated cells...
June 12, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38876226/fangchinoline-inhibits-mouse-oocyte-meiosis-by-disturbing-mpf-activity
#20
JOURNAL ARTICLE
Shi-Cai Gao, Ming-Zhe Dong, Bing-Wang Zhao, Sai-Li Liu, Jia-Ni Guo, Si-Min Sun, Yuan-Yuan Li, Yuan-Hong Xu, Zhen-Bo Wang
Fangchinoline (FA) is an alkaloid derived from the traditional Chinese medicine Fangji. Numerous studies have shown that FA has a toxic effect on various cancer cells, but little is known about its toxic effects on germ cells, especially oocytes. In this study, we investigated the effects of FA on mouse oocyte maturation and its potential mechanisms. Our results showed that FA did not affect meiosis resumption but inhibited the first polar body extrusion. This inhibition is not due to abnormalities at the organelle level, such as chromosomes and mitochondrial, which was proved by detection of DNA damage and reactive oxygen species Further studies revealed that FA arrested the oocyte at the metaphase I stage, and this arrest was not caused by abnormal kinetochore-microtubule attachment or spindle assembly checkpoint activation...
June 12, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
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