Xinyu Zhang, Dan Ma, Baoqin Xuan, Debing Shi, Jie He, Minhao Yu, Hua Xiong, Yanru Ma, Chaoqin Shen, Fangfang Guo, Yingying Cao, Yuqing Yan, Ziyun Gao, Tianying Tong, Xiaoqiang Zhu, Jing-Yuan Fang, Haoyan Chen, Jie Hong
Long non-coding RNAs (lncRNAs) play important roles in carcinogenesis. However, the effect of lncRNA on chemoresistance and RNA alternative splicing remains largely unknown. In this study, we identified a novel lncRNA, CACClnc, which was upregulated and associated with chemoresistance and poor prognosis in colorectal cancer (CRC). CACClnc promoted CRC resistance to chemotherapy via promoting DNA repair and enhancing homologous recombination in vitro and in vivo. Mechanistically, CACClnc specifically bound to Y-box binding protein 1 (YB1, a splicing factor) and U2AF65 (a subunit of U2AF splicing factor), promoting the interaction between YB1 and U2AF65, and then modulated alternative splicing (AS) of RAD51 mRNA, and consequently altered CRC cell biology...
March 11, 2023: Oncogene