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Biomedical Chromatography: BMC

Cuiyun Wang, Fei Jia, Yafei Zhang
The purpose of this study was to develop and validate an LC-MS/MS method for simultaneous determination of idelalisib and GS-563117 in dog plasma. The analytes were extracted using ethyl acetate and then separated on a Waters ACQUITY UPLC BEH C18 column (50 × 2.1 mm, i. d., 1.7 μm) using 0.1% formic acid in water and acetonitrile as mobile phase at a flow rate of 0.3 mL/min in gradient elution mode. The analytes were quantified using selected reaction monitoring (SRM) with precursor-to-product transitions at m/z 416...
February 18, 2019: Biomedical Chromatography: BMC
Yantong Zhu, Haitao Yu, Tengfei Li, Duo Li, Li Ding, Chang Shu
A sensitive liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous determination of ramelteon and its active metabolite M-II in human plasma. After extracted from 200 μL of plasma by protein precipitation, the analytes and internal standard (IS) diazepam were separated on a Hedera ODS-2 (5 μm, 150 mm × 2.1 mm) column with a mobile phase consisted of methanol-0.1% formic acid in 10 mM ammonium acetate solution (85:15, v/v) delivered at a flow rate of 0...
February 17, 2019: Biomedical Chromatography: BMC
Zhengxiang Xia, Lei Lv, Xuemei Di, Jiyang Xue, Zhen Gao, Guoqing Zhang, Hai Zhang
Ermiao pill (EMW), a traditional Chines medicine (TCM), composed of two herb pair phellodendri cortex (PC) and atractylodis rhizome (AR), used for the treatment of PID and inflammatory related diseases. However, the underlying mechanism was still margin. The compatibility played a crucial role in the complex drugs such as TCM, it wasn't well clear. Now we proposed a compositive strategy, which integrated pharmacokinetics and network pharmacology to explore the compatibility in EMW. Firstly, a simple, rapid, and selective method based on UPLC-MS/MS was established and validated for simultaneous qualification of six alkaloids in rat plasma, which was used for a comparative pharmacokinetic study of EMW and its constituted herb PC...
February 12, 2019: Biomedical Chromatography: BMC
Jia Liu, Hanqing Liu, Qingqi Zeng
The aim of this study was to investigate the effect of naringenin on the pharmacokinetics of ibrutinib in rats. A simple and sensitive quantitation method based on ultra-high performance liquid chromatography-Q-Exactive Orbitrap tandem mass spectrometry was developed and validated for t determination of ibrutinib in rat plasma. The samples were extracted using ethyl acetate containing 1% triethylamine and the separated on a Waters ACQUITY UPLC BEH C18 column with acetonitrile and water containing 0.1% formic acid as mobile phase...
February 12, 2019: Biomedical Chromatography: BMC
Xiaoxiao Gao, Jing Miao, Xinhua Guo, Yan Song, Tingting Lv, Luqi Huang, Wenyuan Gao, Xia Li
Gastrodia elata, as a natural plant with nourishing and edible value, its quality and pharmacological activity play a vital role in food and pharmaceutical production. In the present study, a novel approach for simultaneous determination of six components based on high performance liquid chromatography (HPLC) coupled with photodiode array detection (PDA) was developed for the quality evaluation of Gastrodia elata from different regions. Meanwhile, antioxidant and alpha-glucosidase inhibitory activities were estimated and compared...
February 11, 2019: Biomedical Chromatography: BMC
Yi Zheng, Ziqing Wang, Gabrielle Lui, Déborah Hirt, Jean-Marc Treluyer, Sihem Benaboud, Radia Aboura, Inès Gana
Levofloxacin, pefloxacin, ciprofloxacin and moxifloxacin are four fluoroquinolones used in the treatment of serious bacterial infections. A concentration-dependent antibacterial activity of fluoroquinolones is well known. Therefore, therapeutic drug monitoring in daily clinical practice is warranted to assure the therapies efficacy and prevent bacterial resistance. The purpose of the present study was to develop a method using high pressure liquid chromatography with ultraviolet detector for simultaneous quantification of these four fluoroquinolones in human plasma...
February 11, 2019: Biomedical Chromatography: BMC
Mevlut Albayrak, Alptug Atıla, Elif Yılmazel Ucar, Omer Araz, Yucel Kadıoglu
Macitentan is an endothelin receptor antagonist commonly used in the treatment of pulmonary arterial hypertension (PAH). A novel, rapid, simple and sensitive UPLC-MS/MS method was developed and validated for pharmacokinetic study and the determination of macitentan in PAH patients. Macitentan and bosentan which is used as internal standard were detected using APCI in positive ion and MRM mode by monitoring the mass transitions m/z 589.1→203.3 and 552.6→311.5, respectively. Chromatographic separation was performed on reverse phase C18 column (5 μm, 4...
February 6, 2019: Biomedical Chromatography: BMC
Weijia Zheng, A M Abd El-Aty, Seong-Kwan Kim, Jeong-Min Choi, Da-Hee Park, Kyung-Hee Yoo, Young-Sun Kang, Jong-Sup Jeon, Ahmet Hacımüftüoğlu, Jae-Han Shim, Ho-Chul Shin
The present study was carried out to determine sixteen antibiotics belonging to seven different groups (tetracyclines, sulfonamides, penicillins, fluoroquinolones, macrolides, lincosamides, and trimethoprims) in duck meat. A solid-phase extraction (SPE) method based on Oasis HLB cartridges coupled with liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI+ /MS-MS) was developed. Solutions of 0.1 M ethylenediaminetetraacetic acid disodium salt (Na2 EDTA) and 2% trifluoroacetic acid (TFA) were used for the preliminary extraction of the target antibiotics from duck meat, and n-hexane was used for purification prior to SPE...
January 31, 2019: Biomedical Chromatography: BMC
Hyun-A Oh, Hyunbeom Lee, Keon Wook Kang, Ji Hye Im, Donghak Kim, Hyun Ok Yang, Byung Hwa Jung
Traditional herbal medicine consists of multiple components. There are interactions among the components, which affects both potency and toxicity. The preparation of herbal medicines can be a cause of interactions between multi-components in herbs. To demonstrate the difference in multi-herb interactions based on the preparation methods, the change in the active components in the different preparations of Socheongryong-tang (SCRT) was evaluated using metabolomics profiling. We performed multi-component profiling of the decoction of SCRT (SCRTD) and individual herb mixture (SCRTM) using ultra-high-performance liquid chromatography coupled with a quadrupole time of flight mass spectrometry (UPLC-QTOF-MS)...
January 30, 2019: Biomedical Chromatography: BMC
Liu Yang, Hai Jiang, Xinyue Guo, Ajiao Hou, Wenjing Man, Xudong Xing, Meiling Yan, Bingyou Yang, Qiuhong Wang, Haixue Kuang
Supercritical fluid chromatography (SFC) is a safe and ecofriendly analytical technique that has not been fully applied to the analysis of traditional Chinese medicine (TCM). This is the first study on the separation of six quality markers (Q-markers) including paeoniflorin, albiflorin, benzoyl paeoniflorin, oxypaeoniflorin, gallic acid, and benzoic acid from raw, wine-baked, and vinegar-baked Paeoniae Alba Radix (PAR) by SFC. Optimum separation was achieved on an HSS C18 SB column (100×3.0 mm, 1.8 μm particles) with a gradient elution of high-purity carbon dioxide as mobile phase A and methanol/acetonitrile (70:30, v/v) with 0...
January 28, 2019: Biomedical Chromatography: BMC
Xiaomin Xu, Xiaohong Ding, Bo Yuan, Weiwei Li, Yimei Wang, Yi Jin, Haiyan Xu
A rapid, simple and sensitive LC-MS/MS method was established and validated for simultaneous quantification of ticagrelor and its active metabolite AR-C124910XX in human plasma. After plasma samples were deproteinized with acetonitrile, the post-treatment samples were chromatographed on a Dikma C18 column interfaced with a triple quadrupole tandem mass spectrometer. Electrospray negative ionization mode (ESI) and multiple reaction monitoring were adopted to assay ticagrelor and AR-C124910XX. Acetonitrile and 5 mM ammonium acetate was used as the mobile phase with a gradient elution at a flow rate of 0...
January 24, 2019: Biomedical Chromatography: BMC
Osama Abdallah, Moustafa El Agamy, Ehab Abdelraheem, Farag Malhat
An analytical method for the determination of buprofezin residues in cabbage and cauliflower was developed and validated using gas chromatography with Ion Trap mass spectrometry (GC/ITMS). The analyte protectant (AP) D-sorbitol was used at a concentration level of 0.5 mgml-1 in acetonitrile instead of in matrix for constructing the calibration curves of the buprofezin standard. The average recoveries ranged from 91.3% to 96.8%, with RSD of ≤ 2.7%. The limit of detection (LOD) and quantitation (LOQ) of the method in cabbage and cauliflower were 1...
January 23, 2019: Biomedical Chromatography: BMC
Rob Turner, Sean Robert Wevrett, Suzanne Edmunds, Marc Brown, Robert Atkinson, Tim Shea
The aim of this investigation was to develop receiver and extraction fluids, and subsequently validate an analytical method to quantify the permeation and penetration of flurbiprofen into human pharynx tissue using a Franz diffusion cell. The solubility and stability of flurbiprofen in a suitable receiver fluid, and a suitable extraction method and fluid to recover and quantitate flurbiprofen from human pharynx tissue, were investigated using high-performance liquid chromatography (HPLC). The potential interference of human pharynx tissue in the receiver fluid was also investigated...
January 22, 2019: Biomedical Chromatography: BMC
Hongwei Zhao, Supan Cheng, Longfei Zhang, Hongjing Dong, Yongqing Zhang, Xiao Wang
Ultra-high pressure extraction combined with high-speed counter-current chromatography was employed to extract and purify wedelolactone and isodemethylwedelolactone from Ecliptae Herba. The operating conditions of ultra-high pressure extraction were optimized using an orthogonal experimental design. The optimal conditions were 80% aqueous methanol solvent, 200 MPa pressure, 3 min extraction time and 1:20 (g/mL) solid/liquid ratio for extraction of wedelolactone and isodemethylwedelolactone. After extraction by ultra-high pressure, the extraction solution was concentrated and subsequently extracted with ethyl acetate, a total of 2...
January 21, 2019: Biomedical Chromatography: BMC
Ganesh S Moorthy, Christina Vedar, Ahila S Moorthy, Janice L Prodell, Athena F Zuppa
Dexmedetomidine (Dex), a highly selective α2 -adrenergic agonist, is used primarily for the sedation and anxiolysis of adults and children in the intensive care setting. A sensitive and selective assay for Dex in pediatric plasma was developed by employing ultra-high liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with d4-Dex as an internal standard. Dex was extracted from 0.1 mL of plasma by μ-elution solid-phase extraction (SPE). Separation was achieved with a Waters XBridge C18 column with a flow rate of 0...
January 21, 2019: Biomedical Chromatography: BMC
Sreekanth Dittakavi, Rakesh Kumar Jat, Ramesh Mullangi
A simple, sensitive and rapid assay method has been developed and validated as per regulatory guidelines for the estimation of enasidenib on mice dried blood spots (DBS) using liquid chromatography coupled to tandem mass spectrometry with electro spray ionization in the positive-ion mode. The method employs liquid extraction of enasidenib from DBS disk of mice whole blood followed by chromatographic separation using 0.2% formic acid:acetonitrile (25:75, v/v) at a flow rate of 1.0 mL/min on an Atlantis dC18 column with a total run time 2...
January 21, 2019: Biomedical Chromatography: BMC
Weijia Zheng, Jeong-Min Choi, A M Abd El-Aty, Kyung-Hee Yoo, Da-Hee Park, Seong-Kwan Kim, Young-Sun Kang, Ahmet Hacımüftüoğlu, Jing Wang, Jae-Han Shim, Ho-Chul Shin
Pesticides, which are used as plant protection products, can enter the food chain, and exposure to these xenobiotics can cause a wide array of health problems in humans. Therefore, the objective of the present study was to develop an analytical method for the simultaneous determination of residual spinosad (sum of spinosyn A and D), temephos, and piperonyl butoxide in porcine muscle, egg, milk, eel, flatfish and shrimp (sampling period: February to June, 2018) using liquid chromatography-triple quadrupole tandem mass spectrometry (LC-MS/MS)...
January 21, 2019: Biomedical Chromatography: BMC
Rafaela Sponchiado, Julia Medeiros Sorrentino, Natália Olegário, Sendy Sales Oliveira, Leticia Malgarim Cordenonsi, Gustavo Pozza Silveira, Alexandre M Fuentefria, Andreas S L Mendez, Martin Steppe, Cássia V Garcia
The purpose of this paper is to describe the glycosylation of ambrisentan (AMB) by cultures of Cunninghamella elegans ATCC 9245. AMB is an endothelin receptor antagonist, which is used to treat pulmonary arterial hypertension. Filamentous fungi are morphologically complex and may exhibit different forms depending on species and the nature of the culture medium. Biotransformation study was conducted to investigate the ability of C. elegans to metabolize AMB. Parameters were optimized by testing on different culture media and concentrations, pH, drug concentration, static and shaking conditions...
January 20, 2019: Biomedical Chromatography: BMC
Hiroaki Yamaguchi, Nariyasu Mano
Membrane transporters are expressed in various bodily tissues and play essential roles in the homeostasis of endogenous substances and the absortion, distribution, and/or excretion of xenobiotics. For transporter assays, radioisotope-labeled compounds have been mainly used. However, commercially available radioisotope-labeled compounds are limited in number and relatively expensive. Chromatographic analyses such as high-performance liquid chromatography with ultraviolet absorptiometry and liquid chromatography with tandem mass spectrometry have also been applied for transport assays...
January 20, 2019: Biomedical Chromatography: BMC
Kouroush Salimi, Çigdem Kip, Ömür Çelikbıçak, Duygu Deniz Usta, Aslı Pınar, Bekir Salih, Ali Tuncel
In this study, poly (vinylphosphonic acid-co-ethylene dimethacrylate), poly (VPA-co-EDMA) capillary monolith was synthesized as a starting material for obtaining a stationary phase for microscale enrichment of phosphopeptides. The chelation of active phosphonate groups with Ti (IV) ions gave a macroporous monolithic column with a mean pore size of 5.4 μm. The phosphopeptides from different sources were enriched on Ti (IV) attached-poly (VPA-co-EDMA) monolith by using a syringe-pump. The monolithic capillary columns exhibited highly sensitive/selective enrichment performance with the phosphoprotein concentrations as low as 1...
January 18, 2019: Biomedical Chromatography: BMC
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