Imtiaz Ali, Dong-Qing Wei, Abbas Khan, Yuanyuan Feng, Muhammad Waseem, Zahid Hussain, Arshad Iqbal, Syed Shujait Ali, Anwar Mohammad, Jianting Zheng
Malonyl-CoA serves as the main building block for the biosynthesis of many important polyketides, as well as fatty acid-derived compounds, such as biofuel. Escherichia coli, Corynebacterium gultamicum, and Saccharomyces cerevisiae have recently been engineered for the biosynthesis of such compounds. However, the developed processes and strains often have insufficient productivity. In the current study, we used enzyme-engineering approach to improve the binding of acetyl-CoA with ACC. We generated different mutations, and the impact was calculated, which reported that three mutations, that is, S343A, T347W, and S350W, significantly improve the substrate binding...
January 29, 2024: Biotechnology and Applied Biochemistry