journal
Journals Biotechnology and Applied Bioc...

Biotechnology and Applied Biochemistry

https://read.qxmd.com/read/38287712/improving-the-substrate-binding-of-acetyl-coa-carboxylase-accb-from-streptomyces-antibioticus-through-computational-enzyme-engineering
#21
JOURNAL ARTICLE
Imtiaz Ali, Dong-Qing Wei, Abbas Khan, Yuanyuan Feng, Muhammad Waseem, Zahid Hussain, Arshad Iqbal, Syed Shujait Ali, Anwar Mohammad, Jianting Zheng
Malonyl-CoA serves as the main building block for the biosynthesis of many important polyketides, as well as fatty acid-derived compounds, such as biofuel. Escherichia coli, Corynebacterium gultamicum, and Saccharomyces cerevisiae have recently been engineered for the biosynthesis of such compounds. However, the developed processes and strains often have insufficient productivity. In the current study, we used enzyme-engineering approach to improve the binding of acetyl-CoA with ACC. We generated different mutations, and the impact was calculated, which reported that three mutations, that is, S343A, T347W, and S350W, significantly improve the substrate binding...
January 29, 2024: Biotechnology and Applied Biochemistry
https://read.qxmd.com/read/38282371/analysis-of-genes-specific-to-the-early-maturation-stage-of-sesamum-indicum-seeds-by-subtraction-method
#22
JOURNAL ARTICLE
Yu Okuno, Akihiro Kishikawa, Hisashi Imakouji, Motonobu Yoshida
The mechanisms regulating the content ratio of unsaturated fatty acid in sesame oil need to be clarified in order to breed novel varieties with high contents of unsaturated fatty acids. Full-length cDNA libraries prepared from sesame seeds 1 to 3 weeks after flowering were subtracted with cDNAs from plantlets of 4 weeks after germination. A total of 1545 cDNA clones was sequenced. The functions of novel genes expressed specifically during the early maturation of sesame seeds were investigated by the transformation of Arabidopsis thaliana...
January 28, 2024: Biotechnology and Applied Biochemistry
https://read.qxmd.com/read/38253987/spontaneous-nanoemulsification-of-cinnamon-essential-oil-formulation-characterization-and-antibacterial-and-antibiofilm-activity-against-fish-spoilage-caused-by-serratia-rubidaea-bfmo8
#23
JOURNAL ARTICLE
Jasmin Suriya Arul Raj, Sheena Aliyas, Yolin Angel Poomany Arul Soundara Rajan, Kasi Murugan, Ponmurugan Karuppiah, Natarajan Arumugam, Abdulrahman I Almansour, Perumal Karthikeyan
The contemporary food industry's uses of nanoemulsions (NEs) include food processing, effective nutraceutical delivery, the development of functional chemicals, and the synthesis of natural preservatives, such as phytocompounds. Although cinnamon essential oil (CEO) is widely used in the cosmetic, pharmaceutical, and food industries, it is difficult to add to aqueous-based food formulations due to its weak stability and poor water solubility. This study describes the formulation of a CEO nanoemulsion (CEONE) by spontaneous emulsification and evaluates its antibacterial and antibiofilm properties against biofilm-forming Serratia rubidaea BFMO8 isolated from spoiled emperor fish (Lethrinus miniatus)...
January 22, 2024: Biotechnology and Applied Biochemistry
https://read.qxmd.com/read/38246886/poly-%C3%AE-glutamic-acid-overproduction-of-bacillus-licheniformis-atcc-9945-a-by-developing-a-novel-optimum-culture-medium-and-glutamate-pulse-feeding-using-different-experimental-design-approaches
#24
JOURNAL ARTICLE
Mehrdad Ebrahimzadeh Kouchesfahani, Ali Bahrami, Valiollah Babaeipour
The commercial production of multifunctional, biocompatible, and biodegradable biopolymers such as poly-γ-glutamic acid via microbial fermentation requires the development of simple and cheap methods for mass production. This study optimized the poly-γ-glutamic acid production of Bacillus licheniformis ATCC 9945a in several steps. At first, the most critical components of the culture medium, including l-glutamic acid, citric acid, and glycerol, were selected by screening nine factors through the Plackett-Burman experimental design and then were optimized using the response surface method and the central composite design algorithm...
January 21, 2024: Biotechnology and Applied Biochemistry
https://read.qxmd.com/read/38246885/intramyocardial-delivery-of-injectable-hydrogel-with-arctigenin-alleviated-myocardial-ischemia-reperfusion-injury-in-rats
#25
JOURNAL ARTICLE
Chengyin Liu, Xuejun Jiang, Lanyu Liang, Han Liu, Li Li, Qing Shan
Arctigenin belongs to a major bioactive component of Fructus arctii and has been found with cardioprotective effects on rats with ischemia-reperfusion (I/R) injury. The application of arctigenin is limited due to poor water solubility and low bioavailability. Hydrogel drug delivery systems can improve the efficacy and safety of drugs, increase drug utilization, and reduce side effects. We hypothesized that hydrogels containing arctigenin would facilitate the effect of arctigenin and alleviate I/R injury in the rat heart...
January 21, 2024: Biotechnology and Applied Biochemistry
https://read.qxmd.com/read/38238920/the-importance-benefits-and-future-of-nanobiosensors-for-infectious-diseases
#26
REVIEW
Th S Dhahi, Alaa Kamal Yousif Dafhalla, Sawsan Ali Saad, Duria Mohammed Ibrahim Zayan, Amira Elsir Tayfour Ahmed, Mohamed Elshaikh Elobaid, Tijjani Adam, Subash C B Gopinath
Infectious diseases, caused by pathogenic microorganisms such as bacteria, viruses, parasites, or fungi, are crucial for efficient disease management, reducing morbidity and mortality rates and controlling disease spread. Traditional laboratory-based diagnostic methods face challenges such as high costs, time consumption, and a lack of trained personnel in resource-poor settings. Diagnostic biosensors have gained momentum as a potential solution, offering advantages such as low cost, high sensitivity, ease of use, and portability...
January 18, 2024: Biotechnology and Applied Biochemistry
https://read.qxmd.com/read/38233730/overproduction-of-bacterial-cellulose-from-acetobacter-xylinum-bpr2001-using-food-industries-wastes
#27
JOURNAL ARTICLE
Sirwan Khanchezar, Valiolah Babaeipour, Atiyeh Sadat Mostafa
In this study, a cost-effective complex culture media containing molasses and corn steep liquor (CSL) was developed for the high production of bacterial cellulose (BC) by investigating the effect of four effective factors on BC production at three levels using Taguchi and combined methods. The predicted and actual values of BC production in optimal conditions by Taguchi and combined methods were 8.41 and 14.52 g/L, respectively. These results showed that the combined method was more suitable for predicting the optimal conditions in the optimization of BC production, the cost of developed culture medium was around 94% cost of HS medium preparation, molasses was the most effective factor in both experimental design methods, and initial pH adjustment had little impact on BC production...
January 17, 2024: Biotechnology and Applied Biochemistry
https://read.qxmd.com/read/38225871/biotechnological-insights-into-extracellular-enzyme-production-by-thermotolerant-fungi-from-hot-springs-and-caves-morphology-pellets-formation-and-protease-production
#28
JOURNAL ARTICLE
Adriana Jazmín Legorreta-Castañeda, Guadalupe Guerra-Sánchez, Karina García-Gutiérrez, Dario Rafael Olicón-Hernández
This study investigates the thermotolerant fungal biodiversity in caves and hot springs, focusing on their potential for extracellular enzyme production, specifically proteases. Samples were collected from the Cardonal region in Hidalgo, Mexico, using three different isolation methods. The study characterizes the morphological diversity of the isolated fungi and identifies various genera, including Aspergillus, Penicillium, Trichoderma, Cladosporium, and Fusarium, based on morphology. The isolated fungi were screened for their ability to produce extracellular enzymes on solid media, with a particular emphasis on proteases due to their industrial significance...
January 15, 2024: Biotechnology and Applied Biochemistry
https://read.qxmd.com/read/38225854/innovations-in-dengue-virus-detection-an-overview-of-conventional-and-electrochemical-biosensor-approaches
#29
REVIEW
Shadan Raza, Renu Poria, Deepak Kala, Nishant Sharma, Anil K Sharma, Nkurunziza Florien, Hardeep S Tuli, Ankur Kaushal, Shagun Gupta
Globally, people are in great threat due to the highly spreading of viral infectious diseases. Every year like 100-300 million cases of infections are found, and among them, above 80% are not recognized and irrelevant. Dengue virus (DENV) is an arbovirus infection that currently infects people most frequently. DENV encompasses four viral serotypes, and they each express comparable sign. From a mild febrile sickness to a potentially fatal dengue hemorrhagic fever, dengue can induce a variety of symptoms. Presently, the globe is being challenged by the untimely identification of dengue infection...
January 15, 2024: Biotechnology and Applied Biochemistry
https://read.qxmd.com/read/38225826/construction-and-characterization-of-a-promoter-library-with-varying-strengths-to-enhance-acetoin-production-from-xylose-in-serratia-marcescens
#30
JOURNAL ARTICLE
Jie Liu, Di Liu, Tingting Sun, Tai-Ping Fan, Yujie Cai
Serratia marcescens is utilized as a significant enterobacteria in the production of various high-value secondary metabolites. Acetoin serves as a crucial foundational compound of development and finds application in a broad range of fields. Furthermore, S. marcescens HBQA-7 is capable of utilizing xylose as its exclusive carbon source for acetoin production. The objective of this study was to utilize a constitutive promoter screening strategy to enhance both xylose utilization and acetoin production in S. marcescens HBQA-7...
January 15, 2024: Biotechnology and Applied Biochemistry
https://read.qxmd.com/read/38225812/enzymatic-properties-of-ornithine-decarboxylase-from-clostridium-aceticum-dsm1496
#31
JOURNAL ARTICLE
Qian Tan, Linbo Gou, Tai-Ping Fan, Yujie Cai
Clostridium aceticum DSM1496 is an acid-resistant strain in which ornithine decarboxylase (ODC) plays a crucial role in acid resistance. In this study, we expressed ODC derived from C. aceticum DSM1496 in Escherichia coli BL21 (DE3) and thoroughly examined its enzymatic properties. The enzyme has a molecular weight of 55.27 kDa and uses pyridoxal-5'-phosphate (PLP) as a coenzyme with a Km  = 0.31 mM. ODC exhibits optimal activity at pH 7.5, and it maintains high stability even at pH 4.5...
January 15, 2024: Biotechnology and Applied Biochemistry
https://read.qxmd.com/read/38212282/production-of-the-prolyl-endoprotease-pep-from-aspergillus-sp-fsde-16-by-solid-state-fermentation-ssf-and-use-for-producing-a-gluten-free-beer
#32
JOURNAL ARTICLE
Thaís Cartaxo de Almeida, Sharline Florentino de Melo Santos, Everaldo Silvino Dos Santos
Beer is a beverage that contains gluten and cannot be consumed by people with celiac disease. In this context, the enzyme prolyl endoprotease (PEP) can be used to reduce the gluten content in beer. The present study aimed to produce the PEP from Aspergillus sp. FSDE 16 using solid-state fermentation with 5 conditions and comparing with a similar commercial enzyme produced from Aspergillus niger in the production of a gluten-free beer. The results of the performed cultures showed that during the culture, the most increased protease activity (54...
January 11, 2024: Biotechnology and Applied Biochemistry
https://read.qxmd.com/read/38185532/in-vitro-and-in-vivo-assessment-of-antimicrobial-enzymatic-and-antifouling-properties-of-self-potent-lichen-symbiotic-bacteria
#33
JOURNAL ARTICLE
Rubavathi Subbaiyan, Ayyappadasan Ganesan
This study aimed to comprehensively assess the antimicrobial, antifouling, and antibiofilm-forming potential of lichen symbiotic bacteria against marine fouling bacterial strains. A total of 50 lichen-associated bacteria (LAB) isolates were successfully characterized and evaluated for their effectiveness in mitigating biofouling caused by various marine biofoulers. Through a battery of biological assays encompassing enzymatic, antagonistic, antimicrobial, and antifouling assays, 15 LAB isolates were identified based on their antagonist activities...
January 7, 2024: Biotechnology and Applied Biochemistry
https://read.qxmd.com/read/38128959/extraction-process-physicochemical-properties-and-digestive-performance-of-red-yeast-rice-starch
#34
JOURNAL ARTICLE
Mengting Bai, Kai Liu, Yulin Wang, Shuguang Hou, Xiaofang Li, Jia Luo
In the present study, taking red yeast rice (RYR) as the raw material, the optimum extraction process of RYR starch was investigated through a single-factor experiment and the Box-Behnken design: The liquid-to-solid ratio was 5 mL/g, the concentration of sodium hydroxide solution was 0.075 mol/L, and the extraction time was 3.1 h. Under these extraction conditions, the extraction rate of starch reached 90.077%. To explore the influence of solid-state fermentation on RYR starch, three different fermentation stages of RYR starch, raw rice starch, semi-gelatinized rice starch, and RYR starch were used as test materials to determine the changes in the physicochemical properties and glycemic index (GI) values of RYR starch during solid-state fermentation...
December 21, 2023: Biotechnology and Applied Biochemistry
https://read.qxmd.com/read/38112040/mucilage-assisted-fabrication-of-molybdenum-trioxide-nanostructures-for-photothermal-ablation-of-breast-cancer-cells
#35
JOURNAL ARTICLE
Reem A Alomari, Jegan Athinarayanan, Vaiyapuri Subbarayan Periasamy, Ali A Alshatwi
Nanostructures have been used for various biomedical applications due to their optical, antibacterial, magnetic, antioxidant, and biocompatible properties. Cancer is a prevalent disease that severely threatens human life and health. Thus, innovative and effective therapeutic approaches are urgently required for cancer. Photothermal therapy (PTT) is a promising approach to killing cancer cells. In this investigation, we developed a low-cost, simple, green technique to fabricate molybdenum trioxide nanostructures (MNs) using Opuntia ficus-indica mucilage as a template...
December 19, 2023: Biotechnology and Applied Biochemistry
https://read.qxmd.com/read/38082547/improving-the-capturing-ability-of-swirl-based-microfluidic-chip-by-introducing-baffle-wall
#36
JOURNAL ARTICLE
Yanping Dang, Qin Zhang, Zhiming Ou, Shuai Hu
Microfluidics technology is promising in developing microparticle manipulation technology due to its nondestructive control and notable adaptability. The manipulation of microparticle based on swirling stagnation point is one of the feasible microfluidics biotechnologies. Aiming to improve the regulation and control of microparticle, baffle wall is introduced into the 2-microchannel flow field. The theory of wall attachment jet is employed to elucidate the effect of baffle wall. Subsequently, finite volume method simulation is conducted by modeling the swirling flow region (SFR), and the swirling strength is calculated to characterize the SFR's particle-capturing ability...
December 11, 2023: Biotechnology and Applied Biochemistry
https://read.qxmd.com/read/38082540/resveratrol-loaded-decellularized-ovine-pericardium-ecm-introduced-for-tissue-engineering
#37
JOURNAL ARTICLE
Mozafar Khazaei, Morteza Alizadeh, Leila Rezakhani
An ideal scaffold for skin tissue engineering should have a suitable potential for antibacterial activity, no hemolysis, sufficient porosity for air exchange, water retention capacity, and a suitable swelling rate to maintain tissue moisture. Considering this issue, our study used decellularized ovine pericardial tissue's extracellular matrix (ECM). These scaffolds were decellularized with sodium dodecyl sulfate (SDS) and sodium deoxycholate (SD) detergents along with vacuum methods. Following imaging with scanning electron microscopy (SEM), analysis of the mechanical properties, and the measurement of the amount of DNA, collagen, and glycosaminoglycan (GAG), our study observed that the three-dimensional (3D) structure of ECM was largely preserved...
December 11, 2023: Biotechnology and Applied Biochemistry
https://read.qxmd.com/read/38062650/inhibition-of-biofilm-quorum-sensing-and-swarming-motility-in-pathogenic-bacteria-by-magnetite-manganese-ferrite-and-nickel-ferrite-nanoparticles
#38
JOURNAL ARTICLE
Yasemin İspirli Doğaç, Alfred Ngenge Tamfu, Selahattin Bozkurt, Mehmet Kayhan, Mustafa Teke, Ozgur Ceylan
Resistance to antibiotics by pathogenic bacteria constitutes a health burden and nanoparticles (NPs) are being developed as alternative and multipurpose antimicrobial substances. Magnetite (Fe3 O4 np), manganese ferrite (MnFe2 O4 np) and nickel ferrite (NiFe3 O4 np) NPs were synthesized and characterized using thermogravimetric analysis, transmission electron microscopy, Fourier transformed infra-red, and X-ray diffraction. The minimal inhibitory concentrations (MIC) ranged from 0.625 to 10 mg/mL against gram-positive (Staphylococcus aureus ATCC 25923 and Enterococcus faecalis ATCC 29212), gram-negative (Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853) and candida (Candida albicans ATCC 10239 and Candida tropicalis ATCC 13803) species...
December 7, 2023: Biotechnology and Applied Biochemistry
https://read.qxmd.com/read/38054375/analysis-of-highly-frequent-point-mutations-in-glycoprotein-c-glycoprotein-n-and-nucleoprotein-of-cchfv
#39
JOURNAL ARTICLE
Neha Kaushal, Manoj Baranwal
Crimean-Congo hemorrhagic fever virus (CCHFV) is classified among top 10 priority pathogens by World Health Organization. CCHFV belongs to Bunyaviridae family and negative sense ssRNA genome composed of three RNA segments: L, M, and S. RNA viruses show higher mutation rate as compared to DNA viruses. To gain deeper understanding of impact of point mutations in CCHFV M and S segment, mutation profiling, homology modeling, and molecular dynamic (MD) simulation were performed. Structural glycoproteins (glycoprotein C [Gc] and glycoprotein N [Gn]) of CCHFV are important for host-virus interaction and genome packaging, whereas CCHFV nucleoprotein (NP) is crucial for viral replication...
December 6, 2023: Biotechnology and Applied Biochemistry
https://read.qxmd.com/read/38054266/evaluation-of-the-relationship-of-cytokines-concentrations-tumor-necrosis-factor-alpha-interleukin-6-and-c-reactive-protein-in-obese-diabetics-and-obese-non-diabetics-a-comparative-study
#40
JOURNAL ARTICLE
Imad Jassim Mohammad, Soheila Kashanian, Ronak Rafipour, Husam Aljwaid, Sadegh Hashemi
Obesity has been linked to a low-grade inflammatory process in the white adipose tissue. Our study aims to detect the relationship between cytokine levels of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and C-reactive protein (CRP) in obese diabetics, compared to obese non-diabetics, Iraqi individuals. Ninety Iraqi adults, 45 type 2 diabetic and 45 non-diabetic obese, were selected as controls. Serum levels of TNF-α, IL-6, CRP, homeostatic model assessment for homeostasis model assessment of insulin resistance (HOMA-IR), body fat, and body mass index (BMI) were measured...
December 6, 2023: Biotechnology and Applied Biochemistry
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