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Biotechnology and Applied Biochemistry

Fernanda Zaninette, Giulliana Aparecida Lopes de Melo Rocha, Rosemeire Aparecida Bom Pessoni, Marcia Regina Braga, Kelly Simões, Rita de Cassia Leone Figueiredo-Ribeiro, Mauricio Batista Fialho
Fructooligosaccharides (FOS) are fructose-based oligosaccharides employed as additives to improve the nutritional and technological properties of foods. The rhizosphere of inulin-accumulating plants from the Cerrado (Brazilian savanna) harbor fungi capable of synthesizing FOS from sucrose through the transfructosylating activity of β-fructosyltransferases and/or β-fructofuranosidases. Here, we investigated the ability of Penicillium janczewskii Zaleski CCIBt 3352, a fungus isolated from the rhizosphere of Chrysolaena obovata (Asteraceae), to produce FOS in medium supplemented with sucrose concentrations of 30, 100 or 150 g L-1 ...
February 13, 2019: Biotechnology and Applied Biochemistry
Yasen Maimaitiyiming, Chang Yang, Yun Wang, Liaqat Hussain, Hua Naranmandura
Aptamers are short, single-stranded nucleic acid (DNA or RNA) oligonucleotides that can be obtained by a technique called systematic evolution of ligands by exponential enrichment (SELEX) in vitro. Due to superior properties such as small size, high binding affinity and stability, they are considered to be feasible tools for diagnosis and treatment of disease. In the current study, we attempted to screen a high affinity DNA aptamer to selectively target the colorectal carcinoma Caco-2 cells by using cell-based SELEX approach...
February 12, 2019: Biotechnology and Applied Biochemistry
Ling Li, Xiaolong Hao, Hang Liu, Wei Wang, Xueqing Fu, Yanan Ma, Qian Shen, Minghui Chen, Kexuan Tang
Artemisia annua is the only natural source of the sesquiterpenoid artemisinin, which is widely used to treat malaria. The phytohormone jasmonic acid (JA) can significantly promote artemisinin biosynthesis in A. annua. AabHLH1 can bind and activate artemisinin biosynthetic genes, such as AaADS and AaCYP71AV1. In this study, we proved that AabHLH1 was responsive to MeJA treatment and highly expressed in glandular trichome-enriched tissues, and that its expression profile was similar to that of AaADS. Yeast two-hybrid assays showed that AabHLH1 interacted with all nine AaJAZ proteins in A...
February 4, 2019: Biotechnology and Applied Biochemistry
Zdeněk Perutka, Miloslav Šufeisl, Miroslav Strnad, Marek Šebela
Undesirable effects of the pathogen Botrytis cinerea include reduced quality and quantity of wine grapes. Winemaking is also complicated by the formation of a protein haze in white wines and oxidative browning of red wines. We analyzed proteins in experimental Moravian white wines characterized by their instability and haze formation in bottles during storage despite prior bentonite treatment. To study the relationship of wine proteins and haze we carried out proteomics on hazy and clear white wines produced with partly or largely botrytized grapes and standard reference wines...
February 4, 2019: Biotechnology and Applied Biochemistry
Lei Lei, Jiangtao Liu, Xiao Ma, Hong Yang, Zhongli Lei
A dual-stimuli-responsive support material for pectinase immobilization through ionic bonding was prepared. Specifically, polystyrene-b-polymethylacrylic (PS-b-PMAA), light- and pH-sensitive polystyrene-(5-propargylether-2-nitrobenzyl bromoisobutyrate)-b-poly(diethylamino)ethyl methacrylate-b-poly(polyethylene glycol methacrylate) (PS-ONB-PDEAEMA-b-PPEGMA) were synthesized through atom transfer radical polymerization (ATRP), click chemistry and hydrolysis. The two parts could self-assemble into the micelles in aqueous solution...
February 4, 2019: Biotechnology and Applied Biochemistry
Xiao Li, Cong Huang, Chao-Qun Xu, Ya-Li Tan, Yu-Di Luo, Kun Zou, Jian-Hua Li, Zhang-Shuang Deng, Zhong Zheng, Han Ye, Xiao-Long Zhang, Nian Zheng
The high cell density culture (HCDC) of baker's yeast FX-2 was investigated in a 50 L(A) automatic bioreactor. Herein, it was found firstly that the Crabtree effect clearly existed in batch fermentation with higher glucose content, then the critical initial glucose content range (≤2.00 g·L-1 ) was reasonably ascertained to effectively avoid Crabtree effect. In next fed-batch fermentations with different strategies, the second strategy (maintain ethanol concentration lower than 0.10% and pH around 4.80) was confirmed more beneficial to yeast growth than the first strategy (keep RS not more than 2...
February 4, 2019: Biotechnology and Applied Biochemistry
Abdalla Ali Salim, Sanja Grbavčić, Nataš aŠekuljica, Maja Vukašinović-Sekulić, Jelena Jovanović, Sonja Jakovetić Tanasković, Nevena Luković, Zorica Knežević-Jugović
Protease, cellulase and α-amylase producing B. subtilis strain was cultivated by solid-state fermentation technique using soybean meal as a substrate. The aim of the present study was to establish a highly efficient enzymes' extraction method as a first stage in downstream processing. The conventional extraction procedure was optimized by determing pH, stirring rate, solid/liquid ratio and time of extraction on enzymes' recoveries from fermented soybean meal. Yields of leached enzymes were compared to the amounts of enzymes that are achieved with ultrasound-assisted extraction (UAE)...
January 29, 2019: Biotechnology and Applied Biochemistry
Amirhossein Ghaemi, Kaveh Abdi, Shohreh Javadi, Masoud Zare Shehneh, Fatemeh Yazdian, Meisam Omidi, Hamid Rashedi, Bibi Fatemeh Haghiralsadat, Omid Asayeshnaeini
Sulfur compounds are essential for many industries and organisms; however, they cause serious respiratory problems for human beings. Therefore, determination of sulfur concentration is of paramount importance. The research approach in the field of detecting contaminants has led to smaller systems that provide faster and more effective ways for diagnosis purposes. In this study, a novel portable amperometric graphene oxide-protein biosensor platform is investigated. The main characteristic of this structure is the implementation of a micro-fluidic configuration...
January 22, 2019: Biotechnology and Applied Biochemistry
Suguna Ponnusamy, Saranya Viswanathan, Abirami Periyasamy, Shenbagarathai Rajaiah
Polyhydroxyalkanoates (PHAs) are one of the most important biopolyesters produced by vast number of microorganisms that exist in the environment. PHAs gained much attention due to its inherent biodegradable and biocompatible ability along with its physico-chemical properties which are similar to those of conventional petroleum-based plastics. In the present study, PHA producing bacterial strains was isolated from earthworm. Initially, four bacterial strains were selected based on the intensity of fluorescence from eighty non-clonal isolates...
January 17, 2019: Biotechnology and Applied Biochemistry
Nima Mosavat, Pooran Golkar, Mayam Yousefifard, Rabia Javed
Thymus species are aromatic plants with diverse applications in food industries and medicine. This study was conducted to evaluate the potential effect of ZnO nanoparticles on callus proliferation and thymol and carvacrol production in three Thymus species, i.e., T. vulgaris, T. daensis, T. kotschyanus and Zataria multiflora. For this purpose, callus induction was performed on Murashige and Skoog (MS) medium containing different plant growth regulators (PGRs). After optimization of callus growth, the effects of different concentrations of ZnO nanoparticles (100 and 150 mgL-1 ) were investigated...
January 16, 2019: Biotechnology and Applied Biochemistry
Satish V Patil, Sunil H Koli, Bhavana V Mohite, Rahul Patil, Rohini R Patil, Hemant P Borase, Vikas S Patil
Naringinase has high industrial importance, still, the progress in naringinase research is quite slow. The unavailability of effective, simple screening method which will be applicable to different microorganisms like bacteria, fungi and actinomycetes are one of the major reason for this gap. Therefore a simple plate assay was developed for effective screening of microorganisms for naringinase by exposing to iodine vapors. This invented plate assay will fill the technological void for simple screening method and will lead to screen more potent industrially important naringinase producing microorganisms...
January 16, 2019: Biotechnology and Applied Biochemistry
N Beukes, R-A Levendal, E Prinsloo, C Frost
β-cells contain a prominent endoplasmic reticulum (ER), disruptions to cell homeostasis and function, activities the unfolded protein response (UPR). Currently, no direct protocols measure the UPR initiation. Current methods to measure ER stress, include the quantification of nitric oxide (indirect method), western blotting and qRT-PCR of downstream components. However, these methods do not account for the overlap with mitochondrial dysfunction. In this study, INS-1E cells were exposed to pro-inflammatory cytokines to induce ER stress, as determined using nitric oxide, thioflavin-T binding and β-cell functionality (insulin production)...
January 15, 2019: Biotechnology and Applied Biochemistry
Junming Luo, Shaohua Peng, Wenbin Bai, Zhaoyu Wu, Zhongshu Shan, Zetao Wu, Xin Yuan, Xiaoming Che, Zhili Duan, Jinwu Peng, Yichun Wang, Shukun Zhang
Oval cells, a kind of hepatic progenitor cell quiescent at normal condition, activates to proliferate and differentiate into hepatocytes under severe and long-term liver injury, which usually raises severe inflammation. However, how oval cell survives in the inflammatory milieu interne is still unclear. TNFα, mimicking inflammatory hepatic milieu interne, was used to treat oval cell line, WB-F344, to test the protective function of matrilin 2. In this study, our data suggested that matrilin 2 prevented TNFα-induced apoptosis in WB-F344 cells via inhibiting ASK1/MKK7/JNK pathway...
January 9, 2019: Biotechnology and Applied Biochemistry
Jian-Bin Shi, Ning Wang, Hong Zhou, Qing-Hua Xu, Gen-Tu Yan
Gibberellins is one kind of important endogenous hormone in plants, and regulates vegetative and reproductive growth of plants. GA2ox is a class of oxidase that plays a regulatory role in the third stage of gibberellins synthesis. In this paper, we cloned the GhGA2ox1 gene from an upland cotton (Gossypium hirsutum L. var. CCRI49). The results showed that, the CDS of GhGA2ox1 is 996 bp. It coded 331 amino acids this protein, which has high homology with GhGA2ox2 and NtGA2ox1. The quantitative real-time PCR showed that, under the conditions of salt and drought stress, the expression of GhGA2ox1 had a higher up-regulation in root, stem and leaf of transgenic plant, compared with non-transgenic plant...
January 8, 2019: Biotechnology and Applied Biochemistry
Yi-Shu Tang, Dongmei Wang, Chunxia Zhou, Shuren Zhang
Bacterial magnetosomes (BMs) are used as carriers for antibodies, enzymes, and nucleic acids. This study aimed to demonstrate the clinical utility of BMs derived from Magnetospirillum gryphiswaldense for use in anti-tumor immunotherapy. Bis(sulfosuccinimidyl) suberate (BS3) was used to prepare BM-anti-4-1BB antibody complexes. We used syngeneic TC-1 mouse models of cancer to investigate whether BMs combined with an anti-4-1BB agonistic antibodies could enhance the therapeutic effects of anti-4-1BB antibodies in localized disease settings...
January 1, 2019: Biotechnology and Applied Biochemistry
Karin Torres-Obreque, Giovanna Pastore Meneguetti, Débora Custódio, Gisele Monteiro, Adalberto Pessoa-Junior, Carlota Oliveira Rangel-Yagui
Crisantaspase is an asparaginase enzyme produced by Erwinia chrysanthemi and used as to treat acute lymphoblastic leukemia (ALL) in case of hypersensivity to E. coli ASNase. The main disadvantages of crisantaspase are the short half-life (10 hours) and immunogenicity. In this sense, its PEGylated form (PEG-crisantaspase) could not only reduce immunogenicity but also improve plasma half-life. In this work, we developed a process to obtain a site-specific N-terminal PEGylated crisantaspase (PEG-crisantaspase)...
December 29, 2018: Biotechnology and Applied Biochemistry
Ilaria Penna, Ennio Albanesi, Rosalia Bertorelli, Tiziano Bandiera, Debora Russo
Ultraviolet (UV) light exposure is the primary factor responsible for skin photoaging, affecting all the skin layers, mainly through the production of reactive oxygen species (ROS), activation of inflammatory responses, and apoptosis. In keeping with this evidence, exogenous supplementation with dietary antioxidants has been shown to provide photoprotective benefits. Moreover, oral administration of hyaluronic acid (HA) has been proved to reduce the signs of aged skin, such as wrinkles, and increase hydration and elasticity...
December 27, 2018: Biotechnology and Applied Biochemistry
Jinhua Zhang, Sanli Zang, Baoqing Bai, Sanhong Fan
Limonin, a compound of highly-oxidized triterpenoids, has potential functions in preventing or slowing the occurrences of many diseases. In this study, five different bacterial strains were isolated and identified from Citrus maxima (Burm.) Merr. cv. Shatian Yu. Morphological characteristics and 16S rRNA gene sequencing identified them as Bacillus spp, in which two limonin-producing endophytes named P and P9 were discovered by high-performance liquid chromatography and mass spectrometry using an inorganic salt medium and two natural media, also the production was greater in natural medium 1 (4...
December 22, 2018: Biotechnology and Applied Biochemistry
Prasun Kumar, Anoth Maharjan, Hang-Bae Jun, Beom Soo Kim
Renewable energy resources are considered to be promising for the development of a sustainable circular economy. Among various alternatives, the microbial route for various biofuels production is quite lucrative. Use of cellulose and lignocellulose for methane, H2 , organic acids, ethanol, and cellulase has been explored a lot in the past few decades. The major leftover or a co-product of these processes belongs to lignin - an aromatic cross-link polymer and one of the most abundant complex compounds on earth...
December 20, 2018: Biotechnology and Applied Biochemistry
Feng Zhou, Jing Ju, Yongjun Fang, Xiaoxuan Fan, Shuguang Yan, Qiang Wang, Pengfang Wei, Fuliang Duan, Feng Miao, Zhenyuan Hu, Maode Wang
The present study aimed to investigate the protective effects of salidroside (SAL) on 1-methyl-4-phenylpyridinium (MPP+ )-induced PC12 cell model for Parkinson's disease. PC12 cells were pretreated with SAL in different concentration and then exposed to MPP+ . To evaluate the effects of SAL on cytotoxicity, the survival rate was tested by MTT asssay and the apoptosis was tested via flow cytometry and western blot. ROS, GSH and MDA were detected to analysis the effects of SAL on oxidative stress. The mRNA and protein levels of inflammatory factors TNF-α and IL-1β were also determined by RT-qPCR and western blot...
December 13, 2018: Biotechnology and Applied Biochemistry
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