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Histology and Histopathology | Page 2

Juliana Knief, Pamela Lazar-Karsten, Ulrich Wellner, Richard Hummel, Christoph Thorns
Tumor-infiltrating lymphocytes (TILs) have commonly been associated with markedly improved prognosis in a variety of human cancers, including carcinomas of the upper and lower gastrointestinal tract. Especially the presence of T-cells (cytotoxic as well as helper cells) seems to define a subgroup of patients with prolonged overall and event-free survival. The density of TILs was assessed via immunohistochemistry for CD8 and CD103 in a population of 228 adenocarcinomas of the esophagogastric junction. Density of CD8+ T-lymphocytes was inversely correlated with depth of tumor infiltration (p=0...
April 2, 2019: Histology and Histopathology
Ondine Lucaciu, Dragos Apostu, Alexandru Mester, Radu Septimiu Campian, Dan Gheban, Richard J Miron
Absorbable atelo-collagen type 1 represents a new approach for guided bone regeneration with several reported advantages such as: osteoblast attachment, proliferation, mineralization potential, absorption of growth factors and inhibition of bacterial pathogen colonization. The aim of this study was to assess the clinical, radiological (preoperative width, re-entry width, gain), Periotest measurements and histologic benefits of atelo-collagen-derived bovine bone grafts (ImploBone) in combination with an atelo-collagen type I barrier membrane (ImploSorb) for guided bone regeneration (GBR) of atrophic alveolar crest in thirteen patients...
April 1, 2019: Histology and Histopathology
Shuhei Otsuki, Oscar Alvarez-Garcia, Martin K Lotz, Masashi Neo
The expression of heparan sulfate endosulfatases (Sulfs) was investigated in the intervertebral disc (IVD) to clarify their role in IVD homeostasis. Sulf-1 and -2 expression was elucidated in normal and degenerated human IVD. Age-related effects on Sulf expression, type II collagen levels, and structural changes were analyzed in IVDs of wild-type (WT) and Sulf-1 knockout (Sulf-1⁻/⁻) mice. The effect of recombinant Sulf-1 (100 ng/ml) and Sulf-1 knockdown on heparan sulfate proteoglycan and collagen expression in ATDC5 cells was examined...
March 29, 2019: Histology and Histopathology
Salvatore Desantis, Maria Mastrodonato, Gianluca Accogli, Giacomo Rossi, Alberto Maria Crovace
Although the use of probiotics in human and animal medicine is growing, their mode of action remains poorly understood. This study examined the effects of a multi-strain probiotic (SLAB51™) on the morphology and carbohydrate composition of mucins secreted by goblet cells of intestinal crypts in growing-finishing pigs. Sections of duodenum, caecum and colon from pigs fed for 12 weeks with an orally administered control basal diet (No-Pro) or one with a probiotic blend (Pro) were processed for microscopic analysis and stained with (1) haematoxylin-eosin for structural and morphometrical investigation; (2) conventional histochemistry (periodic acid-Schiff, Alcian Blue pH 2...
March 27, 2019: Histology and Histopathology
Umar Nishan, Rafaela Rosa-Ribeiro, Carlos Lenz Cesar, Hernandes F Carvalho
The high incidence of prostatic diseases, including malignant tumors, makes the understanding of prostate biology very important. Androgen deprivation, blockade by orchiectomy, or chemical castration causes prostate and tumor shrinkage. The gene networks involved in a cell type-specific fashion are rather unknown. This work was undertaken to identify genes with annotated function in transcription regulation that might define transitions in gene expression. A total of 15 potential regulatory genes were identified...
March 26, 2019: Histology and Histopathology
Diletta Francesca Squarzanti, Tiziana Cena, Rita Sorrentino, Mario Migliario, Annalisa Chiocchetti, Lia Rimondini, Barbara Azzimonti, Guido Valente
AIMS: to evaluate OPN, MCM7, Ki-67, p53, Bcl-2 and 53BP1 presence, together with the abnormal adaptive CD4 and CD8 T-cell response markers expression in a series of oral lichen planus (OLP) affected patients and assess their combined contribution for a more objective disease classification. METHODS AND RESULTS: In this ex-vivo retrospective analysis, biopsy specimens from 28 adults with a clinical diagnosis of OLP at different progression degree (16 reticular, 2 plaque-like, 1 erosive and 9 mixed type) were collected...
March 25, 2019: Histology and Histopathology
Wen-Ji Jia, Yun Yuan, Chun-Yun Wu
Cerebral ischemia affects many especially with the ageing population. The ensuing ischemic reactions include oxidative stress, inflammation, and excitotoxicity among others. In the search for effective therapeutic strategies for cerebral ischemia, activated microglia which are the key player in neuroinflammation are now recognized as a potential therapeutic target. Microglia possess both neurotoxic and neuroprotective roles. They are protective by continuously surveilling the microenvironment, phagocytosing dead cells, secreting trophic factors and sculpting the neuronal connections by removing axons and pruning excess synapses...
March 22, 2019: Histology and Histopathology
Xijiao Yu, Shuang Liu, Xue Chen, Yanmei Du, Xiaochun Yin, Yi Du, Shu Li
The study is to verify whether Calcitonin gene related peptide (CGRP) gene-modified rat bone mesenchymal stem cells (BMSCs) are promising seed cells with great potential to repair bone defect. A recombinant lentiviral vector overexpressing CGRP peptide, pLenO-DCE-CGRP, was constructed and transfected into rBMSCs to obtain stable CGRP expression. Differently transfected rBMSCs: CGRP- modified rBMSCs (CGRP group), empty vector- modified rBMSCs (Vector group) and normal rBMSCs (Control group) were transferred to collagen scaffold to repair rat skull defects (n=6)...
March 21, 2019: Histology and Histopathology
Qing Li, Aihua Zhang, Changying Xing, Yanggang Yuan
Chronic kidney disease (CKD) is recognized as a worldwide health problem. Progression of CKD may lead to many serious complications, which are associated with increased morbidity and mortality. Presently, there is no satisfactory treatment. Thus, targeted therapies are urgently needed. The kidneys are second to the heart in terms of mitochondrial abundance and oxygen consumption. Thus, it is not surprising that mitochondrial homeostasis is absolutely essential for the normal function of the kidney. In fact, a number of reports indicate that mitochondria are involved in the progression of CKD...
March 20, 2019: Histology and Histopathology
Óscar Mariño-Crespo, Elisa Cuevas-Álvarez, Amy L Harding, Craig Murdoch, Almudena Fernández-Briera, Emilio Gil-Martín
Aims and experimental design. The acute-phase protein haptoglobin (Hp) has been recently detected in colorectal cancer (CRC) tissue, where its expression correlates with metastasis. Recently, we identified Hp as a CDw75 antigen-expressing protein in colorectal tissue. To deepen the knowledge of this protein in CRC, we studied the expression of Hp in healthy and tumour tissue specimens from 62 CRC patients by immunohistochemistry and Western blotting, as well as in the Caco-2 and HT-29 CRC cell lines by quantitative PCR, immunofluorescence microscopy and flow cytometry...
March 13, 2019: Histology and Histopathology
Kanae Nosaka, Karen Makishima, Tomohiko Sakabe, Yohei Yurugi, Makoto Wakahara, Yasuaki Kubouchi, Yasushi Horie, Yoshihisa Umekita
Micropapillary carcinoma (MPC), a relatively rare histologic carcinoma observed in various organs, is associated with vascular invasion, nodal metastasis, and poor prognosis. MPC is different from papillary carcinoma as it has no fibrovascular core and is thus considered essentially hypovascular. MPCs are known to upregulate glucose transporter 1 (GLUT1) via the activation of a transcription factor, hypoxia-inducible factor (HIF)-1. Here we evaluated the expression of nutrient transporters in MPCs to gain a better understanding of the system used by MPCs to compensate for their intrinsic poor vascularity...
March 11, 2019: Histology and Histopathology
Koji Okudela, Hiromasa Arai, Hideya Kitamura, Tomohisa Baba, Hideaki Mitsui, Takehisa Suzuki, Misaki Sugiyama, Emi Honda, Mai Matsumura, Tamiko Takemura, Toshiaki Kataoka, Chihiro Koike, Yoko Tateshi, Michihiko Tajiri, Takashi Ogura, Kenichi Ohashi
A normal counterpart and precancerous lesion that non-terminal respiratory unit (TRU) lung adenocarcinomas (LADCs) develop from have not been clarified. Non-TRU LADCs specifically express hepatocyte nuclear factor 4α (HNF4α). Thus, we have been interested in airway epithelial cells that express HNF4α as the potential precursor of non-TRU LADC. The purposes of the present study are to report the frequency and distribution of HNF4α-expressing cells at the different airway levels, and to investigate the potential significance of the expression of HNF4α in the histogenesis of non-TRU LADC with a special reference to the relationship to bronchiolar metaplasia in idiopathic interstitial pneumonia...
March 8, 2019: Histology and Histopathology
Huifang Zhan, Juan Jin, Shikai Liang, Li Zhao, Jianguang Gong, Qiang He
BACKGROUND: Diabetic kidney disease (DKD), one of the most common causes of end-stage renal disease(ESRD), remains prevalent in many populations. Podocyte loss and apoptosis play a crucial role in the progression of DKD. Tripterygium glycoside (TG), a widely used Chinese herb, exerted comprehensive protective effects on preventing DKD progression. This study was performed to assess the podocyte protective effect of tripterygium glycoside on DKD by the potential role of activation of autophagy and downregulating β-arrestin-1...
March 6, 2019: Histology and Histopathology
Rosa María Vigueras-Villaseñor, Tania Jiménez Cabrera, Margarita Chávez Saldaña, Francisco Jiménez Trejo, Osvaldo Cuevas Alpuche, Julio César Rojas-Castañeda
Cryptorchidism (CO) is a risk factor for infertility in men. It is associated with an increase in oxidative stress which alters the differentiation of the gonocytes to spermatogonia. Epigallocatechin-3-gallate (EGCG) is an antioxidant that acts as a free radical scavenger and activates the antioxidant enzymes. The aim of this work was to investigate if EGCG plays a role in the protection of the testicle from alterations generated by CO and its possible mechanism. Male rabbits 7 days old were divided into four groups and distributed as follows: 1) control (C) treated with EGCG vehicle (V) (C/V); 2) C with administration of EGCG from 65 to 120 days postpartum (dpp) (C/EGCG); 3) CO induced by administration of 17β-estradiol plus EGCG vehicle (CO/V) and 4) CO plus EGCG administration (CO/EGCG)...
March 4, 2019: Histology and Histopathology
Javier Martín-López, Consuelo Pérez-Rico, Natalio García-Honduvilla, Julia Buján, Gemma Pascual Gemma
INTRODUCTION: Pterygium is a conjunctival fibrovascular tissue growth on the cornea. The pathogenesis of pterygium involves several factors such as the presence of active angiogenic factors. Expansion of the lymphatic microvasculature has also been hypothesized. This study examines the activity of the angiogenic/lymphangiogenic factor VEGF and the expression of vascular and lymphatic endothelial proteins in pterygia and normal conjunctival tissues. MATERIAL AND METHODS: Primary grade 2 pterygium (n=20) and normal conjunctiva (n=20) biopsies were obtained during surgery after written informed consent...
March 1, 2019: Histology and Histopathology
Shih-Chiang Huang, Hsuan-Ying Huang
Solitary fibrous tumor (SFT) is a distinct fibroblastic neoplasm of intermediate biological potential, prototypically presenting as a pleura-associated tumor characterized by patternless proliferation of generally banal oval to spindle cells with hemangiopericytoma-like staghorn vessels in fibrocollagenous stroma. Over the past decades, the clinicopathological spectrum of SFT has been ever-expanding with the incorporation of cases exhibiting myxoid, giant cell-containing, and fat-forming histology, as well as those from extrathoracic sites, including the meninx...
April 2019: Histology and Histopathology
Jasna Metovic, Chiara Gallino, Eugenio Zanon, Riccardo Bussone, Roberto Russo, Elena Vissio, Laura Annaratone, Luca Conti, Mauro Papotti, Paola Cassoni, Isabella Castellano
Eccrine spiradenoma is a rare lesion originating from eccrine sweat glands, with only few cases reported in breast tissue: we here describe for the first time, an eccrine spiradenoma arising in the nipple. An 84 year-old woman with a lesion enlarging her right nipple, showing ulcerations and eczema-like changes of the covering skin, was admitted to our hospital. Surgical excision of the central quadrant with nipple-areola complex was performed, followed by histopathological evaluation which revealed an adenoma with predominantly basaloid epithelial cells...
February 26, 2019: Histology and Histopathology
Lourdes Florensa, Leonor Arenillas, Xavier Calvo, Encarnación Pérez-Vila, Sara Montesdeoca, Ana Ferrer, Soledad Woessner
The diagnosis of myelodysplastic syndromes is based on the presence of cytopenias, dysplastic morphological features on peripheral blood (PB) and bone marrow (BM), cytogenetic abnormalities and requires to rule out other diseases resembling these conditions. Optical cytomorphology is the cornerstone of diagnosis of MDS. The recognition of cytological myelodysplasia has a crucial value in diagnosis and prognosis of MDS. Assessment of cytological morphology requires, like other diagnostic techniques (flow cytometry, cytogenetics, histological morphology), experienced observers and the availability of high quality and properly stained samples...
February 19, 2019: Histology and Histopathology
Philipp Pieroh, Angela Ehrlich, Chalid Ghadban, Ludmilla Litvak, Hanno Steinke, Christoph Josten, Johannes Karl Maria Fakler, Faramarz Dehghani
The histological processing of musculoskeletal tissue might be challenging. The alteration of tissue composition e.g. by calcification of soft tissue in the elderly, after trauma or surgical interventions makes the histological processing of fixed tissue difficult. Additional steps of decalcification are then needed that probably affect the staining quality. In the present work, the effects of different decalcification agents and the intermedium methyl benzoate on histological staining methods and immunohistochemistry have been compared...
February 13, 2019: Histology and Histopathology
Edite Vartina, Mara Pilmane, Romans Lacis
INTRODUCTION: One of the risk factors for cardiovascular disease is inflammation. The role that it plays in the pathogenesis of cardiovascular disease remains a topic of ongoing research. The aim of this study was to identify the appearance and distribution of inflammatory markers, interleukins 1α (Il-1α) and 10 (Il-10) and β defensins 2 (βD2), 3 (βD3), and 4 (βD4), in the right atrial tissue from different acquired heart diseases. METHODS: During cardiac surgery, right atrial tissue fragments were taken from 23 patients with acquired heart diseases...
February 8, 2019: Histology and Histopathology
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