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Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire

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https://read.qxmd.com/read/30670905/genetic-characterization-of-a-hantavirus-isolated-from-heilongjiang-province-china
#1
Yulong Wang, Suya Cao, Cheng Cheng, Wendong Ju, Yuping Hua
Hantavirus is the causative agent of hemorrhagic fever with renal syndrome (HFRS). Heilongjiang Province is experiencing an epidemic of HFRS, the main causative agent is a variant of hantavirus called Seoul virus (SEOV). In this study, the entire genome of one SEOV, the DN2 strain, was sequenced and analyzed. The alignment analysis of the sequences indicated that the DN2 strain shares the highest homology with the SEOV-LYO852 strain. The nucleotide identity is 97.6% for the S segment, 97.7% for the M segment, and 98...
January 2019: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://read.qxmd.com/read/30670904/protective-efficacy-of-an-inactivated-brucella-abortus-vaccine-candidate-lysed-by-gi24-against-brucellosis-in-korean-black-goats
#2
Wong-Kyong Kim, Ja-Young Moon, Jeong-Sang Cho, Enkhsaikhan Ochirkhuyag, Md Rashedunnabi Akanda, Byung-Yong Park, Jin Hur
The efficacy of GI24-lysed Brucella abortus cells as a vaccine candidate against brucellosis in goats was evaluated on 2 groups of Korean black goats. Group A goats were immunized subcutaneously (SC) with sterile phosphate-buffered saline, whereas group B goats were immunized SC with approximately 3 × 109 lysed B. abortus cells. Subcutaneous immunization with the lysed cells did not cause any negative impact on the overall clinical status, such as behavior and appetite, throughout the study period. The enzyme-linked immunosorbent assay (ELISA) optical densities values for B...
January 2019: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://read.qxmd.com/read/30670903/comparison-of-4-commercial-modified-live-porcine-reproductive-and-respiratory-syndrome-virus-prrsv-vaccines-against-heterologous-korean-prrsv-1-and-prrsv-2-challenge
#3
Taehwan Oh, Hanjin Kim, Kee Hwan Park, Siyeon Yang, Jiwoon Jeong, Seeun Kim, Ikjae Kang, Su-Jin Park, Chanhee Chae
The efficacy of 4 commercial porcine reproductive and respiratory syndrome virus (PRRSV) modified-live vaccines (MLV), against PRRSV-1 and PRRSV-2 was evaluated and compared in growing pigs. Two of the vaccines were based on PRRSV-1 and two on PRRSV-2. There were no significant differences between each of the two PRRSV-1 MLV vaccines and the two PRRSV-2 MLV vaccines respectively based on virology, immunological, and pathological evaluations. Vaccination with either of the PRRSV-1 MLV vaccines resulted in reduced PRRSV-1 but not PRRSV-2 viremia...
January 2019: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://read.qxmd.com/read/30670902/peripartum-metabolic-profiles-in-a-holstein-dairy-herd-with-alarm-level-prevalence-of-subclinical-ketosis-detected-in-early-lactation
#4
Shin Oikawa, Hanan K Elsayed, Chihoko Shibata, Kyoko Chisato, Ken Nakada
The aim of this study was to characterize peripartum metabolic profiles, including the insulin sensitivity index, in cows diagnosed with subclinical ketosis (SCK) in the early stage of lactation. Cows that calved from January 2011 through December 2014 on a dairy farm with alarm level prevalence of SCK in Hokkaido, Japan ( n = 175) were used. Blood and body condition scores (BCS) were obtained at regular health examinations in 2 consecutive periods, the first between 14 and 2 d before parturition, and the second between 3 and 14 d after parturition...
January 2019: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://read.qxmd.com/read/30670901/comparative-molecular-analysis-of-fecal-microbiota-of-bobcats-lynx-rufus-and-domestic-cats-felis-catus
#5
David Eshar, Charlie Lee, J Scott Weese
The goal of this study was to explore and describe fecal microbiota of captive and wild bobcats (Lynx rufus) and compare the results to those of domestic cats (Felis catus) . Fecal samples from 27 bobcats (8 wild, 19 zoo-kept) were used for novel bacterial deoxyribonucleic acid (DNA) identification using next-generation sequencing of the V4 region of the bacterial 16S ribosomal ribonucleic acid (rRNA) gene, analyzed by Illumina sequencing, and then compared to data obtained from a colony of 10 domestic cats...
January 2019: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://read.qxmd.com/read/30670900/changes-in-antimicrobial-susceptibility-profiles-of-mycoplasma-bovis-over-time
#6
Hugh Y Cai, Rebeccah McDowall, Lois Parker, Emily I Kaufman, Jeff L Caswell
Mycoplasma bovis is a major cause of pneumonia, arthritis, and mastitis in cattle and can lead to significant economic losses. Antimicrobial resistance is a concern and further limits the already short list of drugs effective against mycoplasmas. The objective of this study was to examine changes in in vitro minimum inhibitory concentrations (MICs) of antimicrobials of aminoglycoside, fluoroquinolone, lincosamide, macrolide, pleuromutilin, phenicol, and tetracycline classes for 210 M. bovis isolates collected from 1978 to 2009...
January 2019: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://read.qxmd.com/read/30670899/use-of-standard-diagnostic-techniques-to-determine-eradication-of-infection-in-experimental-equine-septic-arthritis
#7
Roman V Koziy, Seiji Yoshimura, Ryan Dickinson, Joanna M Rybicka, Igor Moshynskyy, Musangu Ngeleka, Jose L Bracamonte, Elemir Simko
Septic arthritis is an important disease in horses, necessitating aggressive and prolonged therapy. In order to guide therapy, reliable methods of detecting the eradication of infection are needed. Therefore, the objective of this study was to investigate detection of eradication of infection in an experimental model of equine septic arthritis using standard diagnostic techniques. For this purpose, 17 adult horses were assigned to 3 experimental groups. The middle carpal joint of each horse was injected with Escherichia coli (Septic group, n = 8), lipopolysaccharide (LPS) (LPS group, n = 6), or sterile saline (Control group, n = 3) at day 0...
January 2019: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://read.qxmd.com/read/30670898/the-effect-of-transfixation-pins-on-the-biomechanical-properties-of-angled-acrylic-connecting-bars
#8
Xavier Montasell, Gregory Herndon, David Szwec, Guy Beauchamp
With acrylic external-fixation frames for fracture repair the acrylic columns can be contoured to allow greater versatility in the placement of transfixation pins, thus minimizing damage to the surrounding soft tissue and making mandibular and transarticular fixation easier. However, contouring affects the stiffness and ultimate strength of the construct under axial compression. In this study, polymethylmethacrylate columns 21 mm in diameter angled at 0°, 30°, 45°, 60°, or 90° with clamps were constructed...
January 2019: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://read.qxmd.com/read/30670897/cochleosaccular-scheibe-dysplasia-in-dogs-a-temporal-bone-study
#9
Nevra Keskin, Hasan Albasan, Irem Gul Sancak, Michael M Paparella, Sebahattin Cureoglu
The objective of this study was to evaluate any otopathologic changes in temporal bone specimens from dogs with deafness related to cochleosaccular (Scheibe) dysplasia (CSD). We used the canine temporal bone collections of the Otopathology Laboratory at the University of Minnesota and of the Massachusetts Eye and Ear Infirmary at Harvard University in Boston. Our morphometric analysis included measuring the areas of the stria vascularis and the spiral ligament and counting the number of spiral ganglion cells...
January 2019: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://read.qxmd.com/read/30670896/evaluation-of-effects-of-radiation-therapy-combined-with-either-pamidronate-or-zoledronate-on-canine-osteosarcoma-cells
#10
Katie Hoddinott, Michelle L Oblak, Geoffrey A Wood, Sarah Boston, Anthony J Mutsaers
Canine osteosarcoma is a devastating disease with an overall poor prognosis. Radiation therapy and bisphosphonates are currently used in combination for palliative treatment, despite a paucity of literature that investigates their combined use. The objectives of this study were to assess the in vitro effects of radiation therapy and bisphosphonates on canine osteosarcoma cells when used in combination. Canine osteosarcoma cell lines D17 and Dharma were treated with radiation and pamidronate or zoledronate, both alone and in combination...
January 2019: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://read.qxmd.com/read/30363385/-salmonella-fecal-shedding-in-pigs-from-birth-to-market-and-its-association-with-the-presence-of-salmonella-in-palatine-tonsils-and-submandibular-lymph-nodes-at-slaughter
#11
Margaret H Ainslie-Garcia, Abdolvahab Farzan, Jane E Newman, Robert M Friendship, Brandon N Lillie
Salmonella is an important cause of foodborne illnesses in humans. Food-producing animals, including swine, are a major source of Salmonella in food products. This study investigated on farm Salmonella fecal shedding in pigs at different production stages - from weaning to marketing - and its association with the presence of Salmonella in tissues at slaughter. Fourteen groups from 8 commercial farrowing sources (N = 809 pigs) were monitored 5 times from birth to slaughter. Fecal and tissue samples were collected from pigs and cultured for Salmonella...
October 2018: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://read.qxmd.com/read/30363384/the-effect-of-graded-concentrations-of-dietary-tryptophan-on-canine-behavior-in-response-to-the-approach-of-a-familiar-or-unfamiliar-individual
#12
James R Templeman, Gary M Davenport, John P Cant, Vern R Osborne, Anna-Kate Shoveller
Tryptophan (Trp) is an indispensable dietary amino acid that supports the modulation of mood and behavior in mammalian species through its role in the serotonergic pathway. This study assessed the behavior patterns of 36 healthy, female adult mixed-breed hound dogs fed a control diet (tryptophan = 0.18% as-fed) or 1 of several experimental diets with graded concentrations of tryptophan (0.05%, 0.1%, and 0.15% of the total diet) supplemented on top of the 0.18% basal level. At baseline, and every 8 weeks throughout the 24-week period, behavioral parameters were evaluated for each dog in response to the approach of an individual familiar to the dogs and another individual who was unfamiliar to the dogs...
October 2018: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://read.qxmd.com/read/30363381/a-method-to-detect-escherichia-coli-carrying-the-colistin-resistance-genes-mcr-1-and-mcr-2-using-a-single-real-time-polymerase-chain-reaction-and-its-application-to-chicken-cecal-and-porcine-fecal-samples
#13
Gabhan Chalmers, Kristin E Davis, Zvonimir Poljak, Robert Friendship, Michael R Mulvey, Anne E Deckert, Richard J Reid-Smith, Patrick Boerlin
Colistin is one of the last-resort antibiotics for the treatment of multidrug-resistant infections in humans, but transmissible colistin-resistance genes have emerged in bacteria from animals. The rapid and sensitive detection among animals of colonization with bacteria carrying these genes is critical in helping to control further spread. Here we describe a method for broth enrichment of colistin-resistant Escherichia coli from animal fecal and cecal samples followed by real-time polymerase chain reaction (PCR) for the simultaneous detection of two of the main colistin-resistance genes, mcr-1 and mcr-2...
October 2018: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://read.qxmd.com/read/30363380/competitive-luminex-immunoassays-for-detection-of-antibodies-to-foot-and-mouth-disease-and-vesicular-stomatitis-viruses-in-multiple-susceptible-hosts
#14
Charles Nfon, Diana Lusansky, Melissa Goolia, Ming Yang, Kate Hole, Leanne McIntyre
Foot-and-mouth disease (FMD) and vesicular stomatitis (VS) cause such similar clinical signs and lesions that laboratory tests are required to distinguish between infections caused by each virus. Using mouse anti-foot-and-mouth disease virus (FMDV) 3B monoclonal or polyclonal anti-vesicular stomatitis virus-New Jersey (VSV-NJ) antibodies and recombinant FMDV 3ABC or VSV-NJ glycoprotein (G) antigens coated to MagPlex beads, competitive Luminex immunoassays (cLIAs) were developed for FMDV and VSV-NJ, respectively...
October 2018: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://read.qxmd.com/read/30363379/comparison-of-saphenous-and-cephalic-blood-lactate-concentrations-in-dogs-with-gastric-dilatation-and-volvulus-45-cases
#15
Liron D Oron, Sigal Klainbart, Yaron Bruchim, Jantina McMurray, Søren Boysen, Matan Saar, Efrat Kelmer
Gastric dilatation and volvulus (GDV) is a life-threatening condition, suspected to result in differential tissue perfusion in different regions of the body. Lactate is a biomarker of hypoperfusion that correlates with treatment outcomes in dogs with GDV. This prospective observational study aimed to compare lactate between saphenous and cephalic venous samples in 45 client-owned dogs with GDV, to evaluate their association with treatment outcomes (survival, surgical complications, and duration of hospitalization) and to assess the correlation between lactate and other biochemical parameters...
October 2018: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://read.qxmd.com/read/30363376/molecular-epidemiology-antimicrobial-susceptibility-and-pulsed-field-gel-electrophoresis-genotyping-of-pseudomonas-aeruginosa-isolates-from-mink
#16
Yongda Zhao, Lili Guo, Jie Li, Binghu Fang, Xianhui Huang
Pseudomonas aeruginosa is an important animal pathogen and contributes to hemorrhagic pneumonia in mink. Between April 2011 and December 2016, samples of lung, liver, and spleen were collected from mink with this disease on 11 mink farms in 5 Chinese provinces. From these samples, we obtained 98 isolates of P. aeruginosa that belonged to 5 serotypes: G ( n = 58), I ( n = 15), C ( n = 8), M ( n = 5), and B ( n = 2); 10 isolates were not typeable (10/98). More than 90% of the isolates formed biofilms, and 85% produced slime...
October 2018: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://read.qxmd.com/read/30363366/use-of-platelet-rich-plasma-for-the-treatment-of-prostatic-cysts-in-dogs
#17
Enrico Bigliardi, Anna Maria Cantoni, Valeria De Cesaris, Laura Denti, Virna Conti, Mara Bertocchi, Francesco Di Ianni, Enrico Parmigiani, Stefano Grolli
Abnormalities of the canine prostate gland, in particular, benign hyperplasia and cysts, are frequent in dogs over 5 years of age. Treatment strategies for dogs with prostatic cysts include ultrasound-guided percutaneous drainage with alcohol sclerotherapy, reduction surgery, and omentalization. The aim of this study was to evaluate the use of platelet-rich plasma (PRP) for the treatment of prostatic cysts in dogs. Ten mixed breed adult male dogs were included in the study. Percutaneous drainage was performed prior to ultrasound-guided PRP injection with a 21G spinal needle...
October 2018: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://read.qxmd.com/read/30363350/hematological-and-serum-biochemical-profile-values-in-pregnant-and-non-pregnant-mares
#18
Babak Faramarzi, Lon J Rich, Jolan Wu
Pregnancy in many mammals, including mares, is associated with physiological changes that are reflected in hematological and biochemical profiles. Understanding those physiological changes and differentiating them from pathological changes is imperative for providing care and medical therapy in pregnant mares. Our objective was to compare normal hematological and biochemical profiles in healthy non-pregnant as well as healthy pregnant mares during the 1st and 2nd trimesters of pregnancy. Blood was collected by jugular venipuncture into ethylenediaminetetraacetic acid and serum tubes...
October 2018: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://read.qxmd.com/read/30363328/effects-of-immunosuppressive-prednisolone-therapy-on-pancreatic-tissue-and-concentration-of-canine-pancreatic-lipase-immunoreactivity-in-healthy-dogs
#19
Hiroshi Ohta, Kazuki Kojima, Nozomu Yokoyama, Noboru Sasaki, Yumiko Kagawa, Kiwamu Hanazono, Tomohito Ishizuka, Keitaro Morishita, Kensuke Nakamura, Satoshi Takagi, Mitsuyoshi Takiguchi
The objective of this study was to examine the effects of immunosuppressive prednisolone therapy on pancreatic tissue and the concentration of serum canine pancreatic lipase immunoreactivity (cPLI) in healthy dogs. Six healthy beagle dogs were subcutaneously administered an immunosuppressive dose of prednisolone [4 mg/kg body weight (BW)] once daily for either 2 or 3 weeks. Serum cPLI concentration was measured before and after treatment. Ultrasonographic examination of the pancreas and laparoscopic biopsy and histopathological examination of the right pancreatic lobe and the liver were also conducted before and after treatment...
October 2018: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://read.qxmd.com/read/30363310/correlation-between-transepidermal-water-loss-tewl-and-severity-of-clinical-symptoms-in-cats-with-atopic-dermatitis
#20
Marcin P Szczepanik, Piotr M Wilkołek, Łukasz R Adamek, Grzegorz Kalisz, Marcin Gołyński, Wiesław Sitkowski, Iwona Taszkun
The aim of this study was to demonstrate whether there is a correlation between transepidermal water loss (TEWL) and the severity of skin lesions in cats with feline atopic dermatitis (AD). The severity of symptoms was determined by using 2 scoring systems for clinically assessing the skin lesions in cats: Scoring Feline Allergic Dermatitis (SCORFAD) and Feline Extent and Severity Index (FeDESI). Such a correlation between TEWL and systems for clinically evaluating symptoms has already been shown in humans and dogs...
October 2018: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
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