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Monika Kubiak, Monika Borkowska, Wojciech Białas, Paulina Korpys, Ewelina Celińska
Fed-batch cultivation is the preferred bioprocessing strategy applied in microbial production of proteins. Feeding strategy is crucial parameters to be optimized upon development of a fed-batch process. In this study, we investigated impact of different feeding strategies on production of recombinant enzymatic protein in Y. lipolytica cultures. From amongst tested strategies, comprising intermittent and continuous feedings, also in cascade with respiratory factors, intermittent feeding executed after complete exhaustion of glycerol from the medium, with moderate amplitude of osmolarity, was the most beneficial in terms of the secretory enzyme amount, its volumetric productivity and specific activity...
February 13, 2019: Yeast
Ravinder Kumar
During the last couple of decades, the methylotrophic yeast, Pichia pastoris has emerged as an important yeast species owing to its increasing importance both in industry and in basic research. The presently available methods for P. pastoris transformation necessitate the preparation of competent cells which requires lots of resource, space, time and efforts. This limits the number of transformations that can be performed by an individual in a given time. This paper is reporting a modification in the available protocols, which makes P...
February 12, 2019: Yeast
Galina Khroustalyova, Gaia Giovannitti, Daria Severini, Rita Scherbaka, Benedetta Turchetti, Pietro Buzzini, Alexander Rapoport
Yeast cells are able to transition into a state of anhydrobiosis (temporary reversible suspension of metabolism) under conditions of desiccation. One of the most efficient approaches for understanding the mechanisms underlying resistance to dehydration-rehydration is to identify yeasts which are stable under such treatments, and compare them with moderately resistant species and strains. In the current study, we investigated the resistance to dehydration-rehydration of six psychrotolerant yeast strains belonging to two species...
February 6, 2019: Yeast
Edgar Velastegui, Chrispian Theron, Julio Berrios, Patrick Fickers
Pichia pastoris is a well-established cell factory for recombinant protein synthesis. Various optimization strategies of processes based on AOX1 promoter have been investigated, including methanol co-feeding with glycerol or sorbitol during the induction stage. Compared to carbon sources, comparatively little research has been devoted to the effects of nitrogen sources. Several reports have described the benefits of adding casamino acids (CA) to the recombinant protein production medium, however without considering its effects at the gene expression level...
January 30, 2019: Yeast
F A Cubillos, B Gibson, N Grijalva-Vallejos, K Krogerus, J Nikulin
The burgeoning interest in archaic, traditional and novel beer styles has coincided with a growing appreciation of the role of yeasts in determining beer character as well as a better understanding of the ecology and biogeography of yeasts. Multiple studies in recent years have highlighted the potential of wild Saccharomyces and non-Saccharomyces yeasts for production of beers with novel flavour profiles and other desirable properties. Yeasts isolated from spontaneously-fermented beers as well as from other food systems (wine, bread, kombucha) have shown promise for brewing application and there is evidence that such cross-system transfers have occurred naturally in the past...
January 30, 2019: Yeast
Quentin Czerwiec, Abdelghani Idrissitaghki, Nabila Imatoukene, Maurice Nonus, Brigitte Thomasset, Jean-Marc Nicaud, Tristan Rossignol
Cyclopropane fatty acids which can be simply converted to methylated fatty acids are good unusual fatty acid candidates for long term resistance to oxidization and low temperature fluidity useful for oleochemistry and biofuels. Cyclopropane fatty acids are present in low amount in plants or bacteria. In order to develop a process for large scale bio-lipids production, we expressed ten cyclopropane fatty acid synthases from various organisms in the oleaginous yeast Yarrowia lipolytica, a model yeast for lipid metabolism and naturally capable of producing large amount of lipids...
January 24, 2019: Yeast
Hans-Werner Mewes
From 1989 to 1997 the Yeast Genome was sequenced by a worldwide international consortium initiated and conducted by André Goffeau (1935-2018). The article describes the pioneering collaboration of yeast scientists from a bioinformatics perspective. Indeed, the yeast genome has turned bioinformatics from an exotic hobby of few nerds into a discipline indispensable for answering biological questions using computational methods.
January 16, 2019: Yeast
Cécile Fairhead, Gilles Fischer, Gianni Liti, Cécile Neuvéglise, Joseph Schacherer
No abstract text is available yet for this article.
January 15, 2019: Yeast
Lisa-Marie Kuhl, Gerben Vader
In meiosis, DNA break formation and repair is essential for the formation of crossovers between homologous chromosomes. Without crossover formation, faithful meiotic chromosome segregation and sexual reproduction cannot occur. Crossover formation is initiated by the programmed, meiosis-specific introduction of numerous DNA double strand breaks, after which specific repair pathways promote recombination between homologous chromosomes. Despite its crucial nature, meiotic recombination is fraud with danger: when positioned or repaired inappropriately, DNA breaks can have catastrophic consequences on genome stability of the resulting gametes...
January 9, 2019: Yeast
Mick Tuite
No abstract text is available yet for this article.
December 18, 2018: Yeast
Kartikeya Joshi, Ling Cao, Philip J Farabaugh
Saccharomyces cerevisiae has been an important model for determining the frequency of translational misreading events, those in which a tRNA pairs incorrectly to the mRNA and inserts an amino acid not specified by the codon in the mRNA. Misreading errors have been quantified in vivo using reporter protein systems or mass spectrometry with both approaches converging on a simple model for most misreading. The available data show that misreading tRNAs must form stereotypical base mismatches that correspond to those that can mimic Watson-Crick base pairs when formed in the ribosomal A site...
December 17, 2018: Yeast
Aisling Y Coughlan, Kenneth H Wolfe
Point centromeres, found in some ascomycete yeasts such Saccharomyces cerevisiae, are very different in structure from the centromeres of other eukaryotes. They are tiny, non-repetitive, and contain only two short conserved sequence motifs. Until recently, point centromeres were thought to have a single evolutionary origin, in the budding yeast family Saccharomycetaceae. Most yeasts outside this family have centromeres that are many kilobases in size. Some have centromeres consisting of a large inverted repeat sequence, others have centromeric clusters of retrotransposons, and a third group including Candida albicans has centromeres with no conserved sequence features...
December 17, 2018: Yeast
Lyne Jossé, Tarun Singh, Tobias von der Haar
One of the central hypotheses in the theory of codon usage evolution is that in highly expressed genes particular codon usage patterns arise because they facilitate efficient gene expression and are thus selected for in evolution. Here we use plasmid copy number assays and growth rate measurements to explore details of the relationship between codon usage, gene expression level, and selective pressure in Saccharomyces cerevisiae. We find that when high expression levels are required optimal codon usage is beneficial and provides a fitness advantage, consistent with evolutionary theory...
December 11, 2018: Yeast
Yukie Misumi, Satoko Nishioka, Akira Fukuda, Takeshi Uemura, Mikiko Nakamura, Hisashi Hoshida, Rinji Akada
In the yeast Saccharomyces cerevisiae, the plasmid YEp, containing a partial sequence from a natural 2 μm plasmid, has been frequently used to induce high levels of gene expression. In this study, we used Japanese sake yeast natural cir0 strain as a host for constructing an entire 2 μm plasmid with an expression construct using the three-fragment gap-repair method without Escherichia coli manipulation. The 2 μm plasmid contains two long inverted repeats, which is problematic for PCR amplification. Therefore, we amplified it by dividing into two fragments, each containing a single repeat together with an overlapping sequence for homologous recombination...
December 7, 2018: Yeast
Oleh Smutok, Maria Karkovska, Tetiana Prokopiv, Taras Kavetskyy, Wladimir Sibirnyj, Mykhailo Gonchar
During the recent decades, a lot of data about the significance of D-lactate determination in food technology and quality control has been accumulated. Nowadays, the development of new methods for the determination of D-lactate is very relevant, especially with regards to biosensors. To construct a D-lactate-selective biosensor, we suggest using the mitochondria of recombinant yeast cells of Ogataea (Hansenula) polymorpha "tr6" (gcr1 catX/Δcyb2, prAOX_DLDH) overproducing D-lactate: cytochrome c-oxidoreductase (DLDH, EC 1...
December 6, 2018: Yeast
Marie-Therese Fröhlich-Wyder, Emmanuelle Arias-Roth, Ernst Jakob
Numerous traditionally aged cheeses are surface ripened and develop a biofilm, known as the cheese rind, on their surfaces. The rind of such cheeses comprises a complex community of bacterial and fungal species that are jointly responsible for the typical characteristics of the various cheese varieties. Surface ripening starts directly after brining with the rapid colonization of the cheese surface by yeasts. The initially dominant yeasts are acid and salt tolerant and are capable of metabolizing the lactate produced by the starter lactic-acid bacteria and of producing NH3 from amino acids...
December 4, 2018: Yeast
María Ángeles Galán-Ladero, María Teresa Blanco-Blanco, María Coronada Fernández-Calderón, Leopoldo Lucio, Yolanda Gutiérrez-Martín, María Teresa Blanco, Ciro Pérez-Giraldo
Candida tropicalis is an emergent pathogen with a high rate of mortality associated with it; however, less is known about its pathogenic capacity. Biofilm formation (BF) has important clinical repercussions, and it begins with adherence to a substrate. The adherence capacity depends principally on the cell surface hydrophobicity (CSH) and, at a later stage, on specific adherence due to adhesins. The ALS family in C. tropicalis, implicated in adhesion and BF, is represented in several CTRG genes. In this study, we determined the biofilm-forming ability, the primary adherence, and the CSH of C...
November 26, 2018: Yeast
Marc Boutry, Henri Batoko, François Chaumont, Michel Ghislain, Charles Hachez, Pierre Morsomme
No abstract text is available yet for this article.
November 23, 2018: Yeast
Young H Ko, Katarzyna Niedźwiecka, Margarida Casal, Peter L Pedersen, Stanisław Ułaszewski
3-bromopyruvate (3BP) is a small, highly reactive molecule formed by bromination of pyruvate. In the year 2000 the antitumor properties of 3BP were discovered. Studies using animal models proved its high efficacy for anti-cancer therapy with no apparent side effects. This was also found to be the case in a limited number of cancer patients treated with 3BP. Due to the 'Warburg effect' most tumor cells exhibit metabolic changes, e.g., increased glucose consumption and lactic acid production resulting from mitochondrial bound overexpressed hexokinase 2...
November 21, 2018: Yeast
Michael Palmgren, Pierre Morsomme
The plasma membrane H+ -ATPase of fungi and plants is a single polypeptide of fewer than 1,000 residues that extrudes protons from the cell against a large electric and concentration gradient. The minimalist structure of this nanomachine is in stark contrast to that of the large multi-subunit FO F1 ATPase of mitochondria, which is also a proton pump, but under physiological conditions runs in the reverse direction to act as an ATP synthase. The plasma membrane H+ -ATPase is a P-type ATPase, defined by having an obligatory phosphorylated reaction cycle intermediate, like cation pumps of animal membranes, and thus this pump has a completely different mechanism to that of FO F1 ATPases, which operates by rotary catalysis...
November 16, 2018: Yeast
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