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Disease Markers

Xinyi Hu, Ming Su, Jun Lin, Lei Zhang, Wen Sun, Jian Zhang, Ye Tian, Wei Qiu
Renal ischemia/reperfusion (IR) injury is one of the most important risk factors for the occurrence of delayed graft function (DGF) after kidney transplantation; however, its mechanism remains not fully understood. In the present study, we screened differentially expressed genes in a murine model of renal IR injury by using high-throughput assays. We identified Corin as one of the most significantly downregulated genes among 2218 differentially expressed genes (≥2-fold, P < 0.05). By using a real-time qPCR assay, we observed that the expression of renal Corin in IR-injured mice was reduced to 11...
2019: Disease Markers
Yuanyuan Yang, Payal Deshpande, Karthik Krishna, Vinodh Ranganathan, Vasanth Jayaraman, Tianhao Wang, Kang Bei, John J Rajasekaran, Hari Krishnamurthy
Background and Aims: Rheumatoid arthritis (RA) and celiac disease (CD) are members of the autoimmune disease family while they have been shown to share multiple aspects in epidemiology and clinical manifestations. The aim of this study was to assess the presence of wheat protein antibodies in RA seropositive subjects and the presence of RA diagnostic markers in subjects with seropositive wheat-related disorders including CD. Methods: Serum samples were collected from 844 subjects with joint pain and/or gastrointestinal symptoms and tested by a CD panel (anti-tTG and anti-DGP), a Wheat Zoomer (WZ) antibody panel (IgG/IgA to 14 wheat proteins), and a RA panel (anti-CCP and anti-RF)...
2019: Disease Markers
Piotr Kamieniak, Joanna Bielewicz, Cezary Grochowski, Jakub Litak, Agnieszka Bojarska-Junak, Beata Daniluk, Tomasz Trojanowski
Objectives: We investigated the influence of spinal cord stimulation (SCS) on IFN- γ , IL-1 β , IL-6, TNF- α , IL-10, and TGF- β serum levels in failed back surgery syndrome (FBSS) patients. The study will try to give new insights into the mechanism of SCS action and the role of IFN- γ and other cytokines in neuropathic pain (NP) development. Materials and Methods: Clinical and biochemical assessment was conducted in four groups of patients: group 0 consisted of 24 FBSS patients qualified to SCS therapy, group 1 included 17 patients who were one month after implantation, group 2 featured 12 patients who were 3 months after the implantation, and group C (the control group) with no NP...
2019: Disease Markers
Jiechao Yang, Liang Zhou, Yanping Zhang, Juan Zheng, Jian Zhou, Zheqiang Wei, Jiaping Zou
Cancer bioinformatics has been used to screen possible key cancer genes and pathways. Here, through bioinformatics analysis, we found that high expression of diaphanous related formin 1 (DIAPH1) was associated with poor overall survival in head and neck squamous cell carcinoma and laryngeal squamous cell carcinoma (LSCC). The effect of DIAPH1 in LSCC has not been previously investigated. Therefore, we evaluated the expression, function, and molecular mechanisms of DIAPH1 in LSCC. Immunohistochemistry and western blot analysis confirmed the significant upregulation of DIAPH1 in LSCC...
2019: Disease Markers
Niclas C Blessin, Ronald Simon, Martina Kluth, Kristine Fischer, Claudia Hube-Magg, Wenchao Li, Georgia Makrypidi-Fraune, Björn Wellge, Tim Mandelkow, Nicolaus F Debatin, Doris Höflmayer, Maximilian Lennartz, Guido Sauter, Jakob R Izbicki, Sarah Minner, Franziska Büscheck, Ria Uhlig, David Dum, Till Krech, Andreas M Luebke, Corinna Wittmer, Frank Jacobsen, Eike-Christian Burandt, Stefan Steurer, Waldemar Wilczak, Andrea Hinsch
TIGIT is an inhibitory immune checkpoint receptor and a putative target for novel immune therapies. Here, we analysed two different types of tissue microarrays of healthy lymphatic and various inflamed tissues, colorectal and lung cancers, as well as >1700 tumour samples from 86 different tumour entities for TIGIT and/or PD-1 by bright field and/or multiplex fluorescence immunohistochemistry. TIGIT was detected in CD8+ cytotoxic T cells, CD4+ T helper cells, FOXP3+ regulatory T cells, and NK cells, but not in CD11c+ dendritic cells, CD68+ macrophages, and CD20+ B lymphocytes...
2019: Disease Markers
Dequan Liu, Yu Yun, Dechun Yang, Xinyu Hu, Xianxiang Dong, Nan Zhang, Lumei Zhang, Hua Yin, Weigang Duan
The main aim of the present study was to investigate the biological function of uric acid. The level of uric acid in different organs in normal male rats was determined with uric acid assay kits, and the expression level of genes in the organs was determined by RNA quantitative sequencing. The correlation analysis between uric acid in the organs and gene expression (measured by FPKM value) was made. Serum uric acid (SUA) in patients with breast cancer or with breast benign tumor was assayed when the diagnosis was made, and SUA in patients with breast cancer was also assayed just after chemotherapy...
2019: Disease Markers
Zheng Zhao, Ke-Nan Zhang, Rui-Chao Chai, Kuan-Yu Wang, Ruo-Yu Huang, Guan-Zhang Li, Yong-Zhi Wang, Jing Chen, Tao Jiang
Background: Researches on immunotherapy of glioblastoma multiforme (GBM, WHO grade IV) have increased exponentially in recent years. As a targeted therapy, a series of biomarkers have been identified in local tumor tissue, while circulating marker which could be detected in the body fluids is still lacking. ADAMTSL4, a secreted glycoprotein, was earlier found to play a critical role in a prognostic signature for primary GBM (pGBM). We aimed to investigate the role of ADAMTSL4 at transcriptome level and its relationship with clinical practice in pGBM...
2019: Disease Markers
Agata Sakowicz, Michalina Lisowska, Lidia Biesiada, Elżbieta Płuciennik, Agnieszka Gach, Magda Rybak-Krzyszkowska, Hubert Huras, Bartosz Sakowicz, Hanna Romanowicz, Agnieszka W Piastowska-Ciesielska, Mariusz Grzesiak, Tadeusz Pietrucha
Background: Preeclamptic pregnancies often present an intensified inflammatory state associated with the nuclear activity of NF κ B. NEMO is an essential regulator of nuclear factor kappa B (NF κ B) in cytoplasmic and nuclear cellular compartments. The aim of the present study is to examine the level and localization of the NEMO protein in preeclamptic and nonpreeclamptic placentas. Methods: The study includes 97 preeclamptic cases and 88 controls. NEMO distribution was analyzed immunohistochemically...
2019: Disease Markers
Xuan-Zhang Huang, Yu-Chong Yang, You Chen, Cong-Cong Wu, Rui-Fang Lin, Zhen-Ning Wang, Xi Zhang
Background: The prognostic value of preoperative anemia in gastric cancer remains unclear. Therefore, the purpose of the present study is to evaluate the prognostic value of preoperative anemia in gastric cancer. Methods: We searched Embase and PubMed databases for relevant studies from inception to March 2018. The prognostic value of preoperative anemia in gastric cancer was determined by calculating the hazard ratio (HR) and the corresponding 95% confidence interval (CI) as effect measures...
2019: Disease Markers
Chien-Yu Huang, Po-Li Wei, Jin-Wun Wang, Precious Takondwa Makondi, Ming-Te Huang, Hsin-An Chen, Yu-Jia Chang
Background: Osteosarcoma (OS) is the most common and most aggressive primary solid malignant bone tumor in children and young adults and has high rates of recurrence and metastasis. The endoplasmic reticulum (ER) stress pathway is important in regulating the chemo-responsiveness of cancer. However, the role of glucose-regulated protein 94 (GRP94) in regulating the response of OS to chemotherapy has never been explored. Methods: In this study, two OS cell lines, MG63 and 143B cells, were used to evaluate the mechanism by which GRP94 modulates the response of osteosarcoma to chemotherapy...
2019: Disease Markers
Xi Wang, Yujie Ning, Pan Zhang, Lei Yang, Cheng Li, Rong Zhou, Xiong Guo
Clinical variables contribute to the severity of Kashin-Beck disease (KBD). However, it is unclear if there is a correlation between gene expression and clinical variables. Peripheral blood samples were collected from 100 patients with KBD and 100 healthy controls from KBD-endemic areas to identify differentially expressed genes in KBD. Correlation analysis and multiple logistic regression analysis were performed using gene expression and clinical parameters. Immunohistochemistry (IHC) was used to detect the expression of related proteins in articular cartilage tissues...
2019: Disease Markers
Agnieszka Mizia-Malarz, Grażyna Sobol-Milejska
Deficiency or impaired function natural killer (NK) cells might result in the development of serious infections and promote the development of malignancies. The aim of our study was to assess the prognostic role of NK cell percentage in bone marrow on the day of acute lymphoblastic leukemia (ALL) diagnosis. 84 children (49 males = 58%; median age 5 yrs) with ALL were enrolled. The NK cell percentage was assessed using flow cytometry with antibodies against the cluster of differentiation (CD): CD3, CD56, and CD16...
2019: Disease Markers
Yunfeng Liu, Lanyan Fu, Lei Pi, Di Che, Yufen Xu, Hao Zheng, Haifeng Long, Lanlan Zeng, Ping Huang, Li Zhang, Tao Yu, Xiaoqiong Gu
Background: Kawasaki disease (KD) is an acute vasculitis disease that commonly causes acquired heart disease in children. Coronary artery aneurysm (CAA) is a major complication of KD. However, the pathogenesis of KD remains unclear. The results of a genome-wide association study (GWAS) showed that two functional single-nucleotide polymorphisms (SNPs; rs699A>G and rs5050T>G) in the angiotensinogen (AGT) gene were related to cardiovascular disease susceptibility. The purpose of our study was to estimate the relationship between the two GWAS-identified AGT gene polymorphisms and the risk of CAA in Southern Chinese children with KD...
2019: Disease Markers
Carmen Mecca, Ileana Giambanco, Rosario Donato, Cataldo Arcuri
The mechanistic target of rapamycin (mTOR) drives several physiologic and pathologic cellular processes and is frequently deregulated in different types of tumors, including glioblastoma (GBM). Despite recent advancements in understanding the molecular mechanisms involved in GBM biology, the survival rates of this tumor are still disappointing, primarily due to the lack of efficacious treatments. The phosphatase and tensin homolog (PTEN)/phosphatidylinositol-3-kinase (PI3K)/protein kinase B (AKT)/mTOR pathway has emerged as a crucial player in GBM development and progression...
2018: Disease Markers
Ning Li, Haiming Wu, Rongxin Geng, Qizhu Tang
Diabetic cardiomyopathy (DCM) is a disorder of the myocardium in diabetic patients, which is one of the critical complications of diabetes giving rise to an increased mortality. However, the underlying mechanisms of DCM remain incompletely understood presently. This study was designed to screen the potential molecules and pathways implicated with DCM. GSE26887 involving 5 control individuals and 7 DCM patients was selected from the GEO database to identify the differentially expressed genes (DEGs). DAVID was applied to perform gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses...
2018: Disease Markers
Zhong-Qing Chen, Ling-Sha Huang, Bo Zhu
Background: The correlation between tumor markers (TM) and TNM stage of non-small-cell lung cancer (NSCLC) has not been widely reported. Methods: TM levels (CEA, CA125, CA15-3, CA19-9, CA72-4, CYFRA21-1, and SCC-Ag) in 424 cases of lung adenocarcinoma (LAC), 166 cases of lung squamous cell carcinoma (LSCC), and 103 cases of benign chest disease (BCD) were analyzed before treatment. Results: By Kendall's tau-b correlation analysis, CEA, CA125, CA15-3, CA19-9, CA72-4, CYFRA21-1, and SCC-Ag were correlated with T stage of LAC ( r = 0...
2018: Disease Markers
Makoto Nagai, Yasutomo Imai, Yoshihiro Wada, Minori Kusakabe, Kiyofumi Yamanishi
Patients with generalized pustular psoriasis (GPP) often present with symptoms that must be differentiated from sepsis. Procalcitonin (PCT) and presepsin (P-SEP) are widely used as biomarkers for sepsis; therefore, we examined the serum PCT and P-SEP levels in patients with psoriatic diseases. The enrolled patients included 27 with psoriasis vulgaris (PV) (22 males, 5 females; mean age 47.7 years), 12 with psoriatic arthritis (PsA) (8 males, 4 females; mean age 51.3 years), and 15 with GPP (10 males, 5 females; mean age 63...
2018: Disease Markers
Ziliang Ye, Haili Lu, Manyun Long, Lang Li
Background: Whether serum uric acid (UA) is associated with all-cause mortality in patients with acute coronary syndrome (ACS) following percutaneous coronary intervention (PCI) remains unclear. Methods: We performed a retrospective cohort study of 2296 patients with ACS. Curve-fitting and Cox proportional-hazard regression models with a hazard ratio (HR) and 95% confidence interval (CI) were used. Results: During a mean follow-up of 246.31 ± 49...
2018: Disease Markers
Abdelkrim Khadir, Sina Kavalakatt, Mohammed Dehbi, Monira Alarouj, Abdullah Bennakhi, Ali Tiss, Naser Elkum
Background: Cardiovascular disease (CVD) risks persist in patients despite the use of conventional treatments. This might be due to chronic inflammation as reflected in epidemiological studies associating circulating low-grade inflammatory markers with CVD recurrent events. Here, we explored this potential link by assessing plasma dual-specificity phosphatase 1 (DUSP1) levels and comparing them to high-sensitivity CRP (hsCRP) and oxidized low-density lipoprotein (oxLDL) levels and their associations to conventional CVD risk factors in confirmed CVD patients...
2018: Disease Markers
Seoung-Jin Hong, Sang Wook Kang, Su Kang Kim, Young Sik Kim, Ju Yeon Ban
Background: Interleukin-1 β (IL-1 β ) plays an important role as a mediator of various inflammatory responses in chronic periodontitis. Several studies have investigated the potential relationship between IL-1 β polymorphism (rs16944) and susceptibility to chronic periodontitis; inflammatory process is involved, but conclusions is still controversial. Objective: The aim of this study was to determine whether the IL-1 β polymorphism (rs16944) is associated with susceptibility to chronic periodontitis...
2018: Disease Markers
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