Yuzhu Zhao, Biying Zhang, Huawei Gu, Tongxin Xu, Qiling Chen, Jin Li, Penghui Zhou, Xueqiang Guan, Ling He, Yanying Liang, Kekun Zhang, Shuwen Liu, Kan Shi
In this study, we conducted a comprehensive investigation into a GH3 family β-glucosidase (BGL) from the wild-type strain of Oenococcus oeni and its mutated counterpart from the acid-tolerant mutant strain. Our analysis revealed the mutant BGL's remarkable capacity to adapt to wine-related stress conditions, including heightened tolerance to low pH, elevated ethanol concentrations, and metal ions. Additionally, the mutant BGL exhibited superior hydrolytic activity towards various substrates. Through de novo modeling, we identified specific amino acid mutations responsible for its resilience to low pH and high ethanol environments...
May 2024: Food Microbiology