Biotechnology & Genetic Engineering Reviews

To explore the effect of acute hypervolemic hemodilution (AHH) with bicarbonated Ringer's solution (BRS) on perioperative serum S100β protein (S100β) and neuron-specific enolase (NSE) in elderly patients undergoing spine surgery. Ninety patients with lumbar spondylolisthesis and fracture surgery admitted to our hospital from January 2022 to August 2022 were selected as the study subjects, and they were randomly and equally divided into group H1 (AHH with BRS), group H2 (AHH with lactated Ringer's solution) and group C (no hemodilution)...

The aim of this study was to investigate the causal relationship between autoimmune disorders and celiac disease (CeD) through Mendelian randomization (MR). Single nucleotide polymorphisms (SNPs) significantly associated with 13 autoimmune diseases were extracted from the summary statistics of European genome-wide association studies (GWAS), and their effects were examined by Inverse variance-weighted (IVW) in a large European GWAS on CeD. Finally, reverse MR was performed to investigate the causal effects of CeD on autoimmune traits...

This study aims to study the effffects of Tiaoshen Changzhi acupuncture on behavior and striatum ΔFosB in rats with Levodopa-induced Dyskinesias (LIDs). In this experimental study, Levodopa-induced Dyskinesia (LID) rat models were established by 6-OHDA double-target injection and randomly assigned to six groups, with ten rats in each group. The rats were subjected to difffferent interventions for 28 days, and their behavior was observed. Additionally, the content of ΔFosB, a marker of neuronal activation, in the rat striatum was detected by immunohistochemistry and qRT-PCR...

To explore the significance of the analysis of pathological characteristics of breast cancer and the detection of myeloid-derived suppressor cell (MDSC) levels in peripheral blood for the evaluation of biological characteristics. 138 breast cancer patients were enrolled as the research group, while 138 patients with benign breast diseases were included as the control group. All patients underwent pathological analysis and detection of peripheral blood MDSCs levels, progesterone receptor (PR), estrogen receptor (ER), human epidermal growth factor receptor 2 (HER-2), and proliferating cell nuclear antigen (Ki-67)...

Acute anterior uveitis (AAU) can cause great pain to patients. Th cytokines in peripheral blood are significantly changed in these patients, including serum interleukin-23 (IL-23), interleukin-17 (IL-17), interleukin-4 (IL-4), interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α) and transforming growth factor-β1 (TGF-β1) increase. However, the relationship between Th cytokines and recurrence of AAU is still unclear. Ninety-two cases of AAU were enrolled from January 2020 to April 2022 in our hospital (observation group)...

The purpose of this study was to assess the effects of psychological interventions on the pregnancy rates of infertile women undergoing assisted reproductive technology (ART). Using the electronic databases PubMed, EM Base, Cochrane Library, WOS, CNKI, WanFang Data, CSTJ, and CBM, a systematic literature search was conducted in the second week of August 2019. Randomized controlled trials (RCTs) on the effect of psychological interventions on the pregnancy rate of infertile women undergoing assisted reproductive technology were collected...

The role of IBA in regulating the recovery of liver cancer was investigated using a rat model of liver cancer and an intraoperative blood return model (IBA). SD rats were used to construct the IBA model. Kupffer cells were isolated from liver cancer tissues, and their biological characteristics were analyzed by flow cytometry. Comet assay was used to detect DNA damage in tumor cells; clone formation assay and transwell assay were used to detect tumor cell proliferation and migration ability. Western blot analysis was used to determine the changes in related signaling pathways...

To analyze psychological and emotional changes in breast cancer patients at different ages before and after surgery based on data. The clinical data of 363 patients undergoing radical mastectomy for breast cancer in our hospital from December 2019 to December 2021 were selected for retrospective analysis. The patients' psychological and emotional changes before and after surgery were evaluated with the mental health symptom self-rating scale, and patients' quality of life was assessed by World Health Organization Quality of Life (WHOQOL)-BREF...

Carotenoids are fat-soluble bio pigments often responsible for red, orange, pink and yellow coloration of fruits and vegetables. They are commonly referred as nutraceutical which is an alternative to pharmaceutical drugs claiming to have numerous physiological benefits. However their activity often get disoriented by photonic exposure, temperature and aeration rate thus leading to low bioavailability and bio accessibility. Most of the market value for carotenoids revolves around food and cosmetic industries as supplement where they have been continuously exposed to rigorous physico-chemical treatment...

OBJECTIVE: To explore the diagnostic value of ultrasound and nerve electromyography (EMG) in the diagnosis of carpal tunnel syndrome (CTS) and its severity in the elderly. METHODS: The data of 140 elderly CTS patients were retrospectively analyzed. The data of 80 patients with other diseases with similar symptoms and high suspicion of CTS during the same period were retrospectively analyzed. Pearson method was used to analyze the correlation between cross-sectional area (CSA) and motor nerve conduction velocity (MCV), distal motor latency (DML), compound muscle action potential (CMAP), sensory conduction velocity (SCV), middle-latency (ML) and sensory nerve action potential (SNAP) levels...

Caudal anesthesia alleviates the strong pain endured by children during surgical treatment for concealed penis. In the traditional method, anesthesiologists identify the puncture point using the 'blind probe' method, which leads to anesthesia induction failure in children. Ultrasound has recently gained wide attention for its guidance in peripheral nerve block analgesia. However, the clinical significance of wireless ultrasound - guided caudal anesthesia technology in children remains unexplored. This study investigated the clinical value of wireless ultrasound - guided caudal anesthesia in children undergoing concealed penis surgery...

The leaves of Rumex vescarius L. are used locally to treat diabetes, a chronic illness. A flavonoid called Luteolin from R. vesicarius was chosen to explore for the antidiabetic potential through the in vivo antidiabetic test against male albino Wistar rats that had been induced with diabetes due to alloxan. Additionally, docking screening was carried out with the aid of autodock software to identify probable moiety that might be in charge of its anti-diabetic effect. Given at a dose of 100 mg/kg body weight, luteolin from R...

Uncoordinated 51-like kinase 1 (ULK1) is an essential part involved in autophagy to maintain cell viability and homeostasis. Herein, the expression levels of ULK1 in colon cancer (CC) were investigated, and its clinicopathological features and potential function were analyzed. Data of ULK1 were obtained from a public database. UCSC XENA RNAseq data were uniformly processed by using the Toil process. STRING was employed for identification of co-expression genes and development of PPI networks whose interaction scores exceeded 0...

This study analyzed sequencing and clinical data from the Cancer Genome Atlas (TCGA) and gene expression synthesis, and used Chinese glioma Genome Atlas (CGGA) data for external validation. The expression of DCP2 in normal brain and tumor tissue was compared. We analyzed the clinical and molecular characteristics and prognostic value of DCP2 in glioma. In addition, DCP2 expression levels were evaluated in 30 glioma tissue samples and upregulated in glioma samples compared to normal brain tissue ( p  < 0...

Immune checkpoint blockade (ICB) has emerged as a promising immunotherapeutic approach for the treatment of various tumors. However, the efficacy of this therapy is limited in a subset of patients, and it is important to develop strategies to enhance immune responses. Studies have demonstrated a critical role of gut microbiota in regulating the therapeutic response to ICB. Gut microbiota composition, diversity, and function are mediated by metabolites, such as short-chain fatty acids and secondary bile acids, that interact with host immune cells through specific receptors...

This study aimed to evaluate the potential of deep learning applied to the measurement of echocardiographic data in patients with sudden cardiac death (SCD). 320 SCD patients who met the inclusion and exclusion criteria underwent clinical evaluation, including age, sex, BMI, hypertension, diabetes, cardiac function classification, and echocardiography. The diagnostic value of deep learning model was observed by dividing the patients into two groups: training group (n=160) and verification group (n=160), as well as two groups of healthy volunteers (n=200 for each group) during the same period...

Through the analysis of the existing case data, this study explored the application effect of predictive nursing combined with bird nest nursing in children with hypoxic ischemic encephalopathy. The data of 120 children with hypoxic-ischemic encephalopathy admitted to our hospital from May 2019 to June 2021 were retrospectively analyzed, and they were divided into A ( n  = 40), B ( n  = 40) and C ( n  = 40) groups according to different nursing methods. Neonatal neurobehavioral assessment (NBNA), Canadian derivatives clearing corporation (CDCC) and Gesell Developmental Schedules (GDS) scores were compared among the three groups...

To explore the analysis on application effect and prognostic factors of medical care combined with nursing in the elderly with type 2 diabetes mellitus (T2DM) and cerebral infraction (CI) based on targeted management mode. The clinical data of 180 elderly patients with T2DM and CI in our hospital from August 2017 to August 2019 were selected for retrospective analysis. Their cognitive function and daily living ability before and after intervention were evaluated, using the National Institutes of Health Stroke Scale (NIHSS) to evaluate their prognosis...

To explore the correlation between pure tone audiometry at all frequencies and distortion product otoacoustic emission (DPOAE) of hidden hearing loss (HHL). The workers exposed to noise from a factory in Zhangjiakou (noise exposure group, n  = 73) and normal young and middle-aged people recruited by society (control group, n  = 77) from August 2021 to April 2022 were study subjects, and all of them underwent audiometry in clinic. Compared with the control group, the noise exposure group had significantly higher threshold by extended high-frequency audiometry at all frequencies and higher signal-to-noise ratio threshold (SNR50) (all P  < 0...

This investigation aimed to elucidate the application impact of prehospital-hospital integrated emergency nursing in individuals with acute cerebral infarction (ACI). Data of 230 ACI patients admitted to our hospital from May 2021 to July 2022 were retrospectively analyzed and divided into A and B groups (AG and BG) according to different nursing methods. The inter-group comparison of the treatment time (time for physician arrival, time for completion of examination, time between admission and thrombolytic therapy, length of stay in emergency) was compared...