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Immunological Investigations

Mohammad Mahdi Eftekharian, Rezvan Noroozi, Alireza Komaki, Mehrdokht Mazdeh, Soudeh Ghafouri-Fard, Mohammad Taheri
Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is a long noncoding RNA (lncRNA) with a possible role in the regulation of immune responses. A previous study has demonstrated down-regulation of this lncRNA in multiple sclerosis (MS) patients. In the current study, we genotyped two MALAT1 single nucleotide polymorphisms (SNPs) in 428 Iranian MS patients and 505 healthy subjects. The G allele of the rs619586 was significantly under-represented in MS patients compared with controls (OR (95% CI) = 0...
March 1, 2019: Immunological Investigations
Fateme Rahmani, Hossein Hadinedoushan, Nasrin Ghasemi
This study determined the roles of OX40 and OX40L in women with recurrent spontaneous abortion (RSA). We compared the expression of OX40 and OX40L genes in peripheral blood mRNA levels and serum levels of OX40L in women with a history of RSA to the control group. In this case-control study, 40 women with a history of RSA (case group), and 40 others with no history of abortion (control group) were investigated. The expressions of OX40 mRNA and OX40L mRNA were determined in the two groups using the quantitative polymerase chain reaction...
February 22, 2019: Immunological Investigations
Yong Zeng
Five different serine protease homologs (SPH) transcripts presumably or possibly resulting from alternative splicing were cloned from the hemocytes of crayfish (Procambarus clarkii) in this paper. Although different deletions of cDNA of SPH-2 and SPH-4 were found in the 5' untranslated regions, they shared the same open reading frame and encoded a 424 amino acids protein with a calculated molecular weight of 45.84 kDa compared with SPH-5. The predicted cutting site of the signal peptide was located between Ala22 and Glu23 ; a clip domain and a trypsin-like serine protease domain were located in the N-terminal and the C-terminal, respectively...
February 22, 2019: Immunological Investigations
Yasmin Nabiel, Alaa Mosbah
BACKGROUND: This study was conducted to compare the levels of maternal serum soluble endoglin (sEng) and cell-free fetal DNA (cffDNA) in pregnant females with PE to normotensive pregnant ones, together with relating these levels to preeclampsia (PE) severity and onset. Method of the study: It was a comparative study in Mansoura University Hospital, Egypt, to detect the levels of serum sEng by ELISA besides the levels of cffDNA by quantitative real-time polymerase chain reaction in 80 pregnant females suffering from PE in addition to 80 normotensive pregnant ones that were included as control...
February 22, 2019: Immunological Investigations
Mengjie Zhang, Jiali Yang, Jian Zhou, Weiwu Gao, Yi Zhang, Yuxin Lin, Huaizhi Wang, Zhihua Ruan, Bing Ni
Programmed death-1 (PD-1), a key immune checkpoint molecule, has been developed as an oncotherapy target for various carcinomas. However, treatment with anti-PD-1 elicited only a minimal effect in pancreatic ductal adenocarcinoma (PDAC). Subsequent studies revealed the existence of a subset of PD-1+ T cells coexpressing CD38 and CD101, representing a fixed dysfunctional subpopulation that are not able to be rescued by anti-PD-1 immunotherapy. However, whether this subpopulation of PD-1 expressing CD8+ T cells could be useful in predicting PDAC stage or prognosing survival is unknown...
January 28, 2019: Immunological Investigations
Amarilla B Mandola, Jacov Levy, Amit Nahum, Nurit Hadad, Rachel Levy, Anna Rylova, Amos J Simon, Atar Lev, Raz Somech, Arnon Broides
Neutrophil chemotactic defects have been reported previously in patients with hyper-IgE syndrome. Bi-allelic mutations in dedicator of cytokinesis 8 (DOCK8) gene usually cause an autosomal recessive hyper-IgE syndrome phenotype. Data are lacking about expression of DOCK8 protein in neutrophils or the possible role of DOCK8 in neutrophil function. We sought to determine if DOCK8 protein is expressed in neutrophils and if DOCK8 plays a role in neutrophil function. The expression of DOCK8 protein was assessed in neutrophils from healthy volunteers with and without activators...
January 28, 2019: Immunological Investigations
Mengya Chen, Xingxing Hu, Meng Wu, Jiajia Yang, Renfang Han, Yubo Ma, Xu Zhang, Yaping Yuan, Rui Liu, Mengmeng Wang, Guangming Jiang, Jixiang Deng, Shengqian Xu, Jianhua Xu, Zongwen Shuai, Faming Pan
OBJECTIVES: To investigate the role of osteoprotegerin (OPG), receptor activator of nuclear factor-kB ligand (RANKL), and RANKL/OPG ratio in the pathogenesis of ankylosing spondylitis (AS). METHODS: Studies that compared serum levels of OPG, RANKL, and RANKL/OPG ratio between AS patients and healthy controls were gathered. Pooled standardized mean differences (SMDs) with 95% confidence intervals (CIs) were calculated by the random-effects model. RESULTS: Twenty studies containing 1592 AS patients and 1064 healthy controls were included in this meta-analysis...
January 28, 2019: Immunological Investigations
Leema George, Tamizhselvi Ramasamy, Kns Sirajudeen, Venkatraman Manickam
Lipopolysaccharide (LPS) induces apoptosis in murine macrophages through the autocrine secretion of tumor necrosis factor (TNF)-α and nitric oxide (NO). LPS-induced inflammation in murine macrophages is associated with hydrogen sulfide (H2 S) production. In this present study, we reported the novel role of H2 S in LPS-induced apoptosis and its underlying molecular mechanism specifically at late phases in murine macrophage cells. Stimulation of RAW 264.7 macrophages with LPS resulted in a time- and dose-dependent induction of apoptosis...
January 28, 2019: Immunological Investigations
Xinying Li, Jocelyn Darby, A Bruce Lyons, Gregory M Woods, Heinrich Körner
INTRODUCTION: Macrophage phagocytosis of pathogens and tumour cells is an important early event in protection against infectious disease and cancer. As tumour necrosis factor α (TNF) is an important cytokine in macrophage activation, we investigated the involvement of TNF in macrophage phagocytosis of tumour cells. METHODS: We used Devil Facial Tumour Disease (DFTD) cancer cells as the target tumour cells. The Tasmanian devil (Sarcophilus harrisii) population is threatened by the transmissible DFTD...
January 19, 2019: Immunological Investigations
Haruka Hirosaki, Yosuke Maeda, Masayuki Shimojima, Ken Maeda, Hiroyuki Iwata, Masahiro Takeyoshi
Anti-TNF antibodies are major therapeutics for rheumatoid arthritis and have been approved for marketing in many countries. Antibody-dependent cellular cytotoxicity (ADCC) is considered to be a potential mechanism of action of anti-TNF antibodies, since some anti-TNF antibodies have been confirmed to induce cytotoxic effects on TNF-producing cells via ADCC and complement-dependent cytotoxicity (CDC) in in vitro experiments. In this study, we established a new stable effector cell line expressing human FcγRIIIa, CD16:KHYG-1, and compared the performance of this cell line with that of peripheral blood mononuclear cells (PBMCs) in ADCC assays against CHO-derived target cells expressing protease-sensitive pro-TNF...
December 20, 2018: Immunological Investigations
Ata Mahmoodpoor, Hadi Hamishehkar, Kamran Shadvar, Zohreh Ostadi, Sarvin Sanaie, Seied Hadi Saghaleini, Nader D Nader
OBJECTIVE: To modulate the inflammatory response in respiratory distress syndrome (ARDS) with selenium. BACKGROUND: Selenium replenishes the glutathione peroxidase proteins that are the first line of defense for an oxidative injury to the lungs. METHODS: Forty patients with ARDS were randomized into two groups: the SEL+ group being administered sodium selenite and the SEL- group receiving normal saline for 10 days. Blood samples were taken on Day-0, DAY-7, and Day-14 for assessment of IL-1 beta, IL-6, C-reactive protein, GPx-3, and selenium...
February 2019: Immunological Investigations
Nasrin Iranshahi, Shirin Assar, Seyed Mojtaba Amiri, Parisa Zafari, Adel Fekri, Mahdi Taghadosi
BACKGROUND: Interleukin-35 (IL-35) is a member of the IL-12 family of heterodimeric cytokines produced by regulatory T (Treg) cells. This immunosuppressive cytokine can prevent exaggerated inflammatory responses like those responsible for the development of rheumatoid arthritis (RA). This study aims to determine the correlation between the gene expression of Epstein-Barr virus-induced gene 3 (EBI-3) and IL-12A (p35) subunits of IL-35 in peripheral blood leukocytes with immunological and clinical parameters in RA patients...
December 4, 2018: Immunological Investigations
Eun-Kyung Moon, Hae-Ji Kang, Ki-Back Chu, Su-Hwa Lee, Dong-Hun Lee, Yunsoo Soh, Fu-Shi Quan
Influenza virus-like particle (VLPs) vaccines are a promising alternative to conventional egg-based vaccines. Evaluation of vaccine efficacy induced by HA-M1 VLPs, NA-M1 VLPs or M1 VLPs against virus challenge infection would provide important insight into vaccine design strategy. In this study, we generated VLPs containing hemagglutinin (HA), neuraminidase (NA) or M1 proteins derived from the A/California/04/09. Mice were immunized intramuscularly with HA-M1, NA-M1 or M1 VLPs and protective immunity was evaluated by assessing lung virus loads against low (5LD50) or high (100LD50) lethal dose of homologous virus challenges...
November 15, 2018: Immunological Investigations
Giacomo Crotti, Francesco Gianfagna, Marialaura Bonaccio, Augusto Di Castelnuovo, Simona Costanzo, Mariarosaria Persichillo, Amalia De Curtis, Chiara Cerletti, Maria Benedetta Donati, Giovanni de Gaetano, Licia Iacoviello
The association between obesity and mortality in the elderly remains controversial. To test the association between BMI and mortality, with the hypothesis of a mediation by low-grade inflammation (LGI), a prospective study design (median follow-up 7.8 years) was used on a sample of 4,970 elderly subjects (age ≥ 65 years) from the Moli-sani Study cohort. The association between BMI categories and overall or cause-specific mortality (hazard ratio, HR) was calculated by multivariable Cox regression. Dose-response relationship was tested using restricted cubic splines...
November 13, 2018: Immunological Investigations
Ibnu A Ariyanto, Riwanti Estiasari, Lukman Edwar, Nandini Makwana, Silvia Lee, Patricia Price
BACKGROUND: Active infections with cytomegalovirus (CMV) increase NK cell expression of the inhibitory receptor LIR-1 and the activating receptor NKG2C in transplant recipients. However, the effects of CMV on NK cells are different in HIV patients stable on antiretroviral therapy (ART) and have not been analyzed in young HIV patients beginning ART. METHODOLOGY: We followed a cohort of 78 Indonesian HIV patients beginning ART. CMV antibodies were measured in plasma before ART (baseline), and after 1, 3, 6, and 12 months...
November 13, 2018: Immunological Investigations
Farshid Fathi, Zahra Faghih, Bijan Khademi, Toomaj Kayedi, Nasrollah Erfani, Abbas Gahderi
INTRODUCTION: Head and neck squamous cell carcinomas (HNSCCs) are the most common cancers of head and neck and the sixth most common malignancy worldwide. Programmed cell death 1 (PD-1) is an immune inhibitory molecule which through interaction with its ligands recruits protein phosphatase resulting in immune response inhibition. Expression of PD-1 ligands on tumor cells is considered as one of the crucial immune evasion mechanisms. This study aimed to investigate the association of PD-1 gene polymorphisms at positions PD1...
October 30, 2018: Immunological Investigations
Miki Nagashima, Madoka Koyanagi, Yutaka Arimura
Allergic diseases have increased in the last three decades. Mast cells play a critical role in allergic diseases along with allergen-specific immunoglobulin E (IgE). Following mast cell degranulation elicited by ligation of the IgE-FcεRI receptor complex with allergen, allergic reactions are followed by various symptoms such as vascular hyperpermeability, mucous secretion, itching, sneezing, wheezing, rashes, fever, and anaphylactic shock. Susceptibility or inclination to allergy varies depending on individual genetic traits and living environment, and it has long been believed that such an inclination is determined by an immunologic balance of T helper cell types...
October 18, 2018: Immunological Investigations
Ana Barić, Luka Brčić, Sanda Gračan, Veselin Škrabić, Marko Brekalo, Marta Šimunac, Vesela Torlak Lovrić, Iva Anić, Maja Barbalić, Tatijana Zemunik, Ante Punda, Vesna Boraska Perica
BACKGROUND: Hashimoto's thyroiditis (HT) is the most common form of autoimmune thyroid disorders characterized by lower production of thyroid hormones and positivity to autoantibodies to thyroglobulin (TgAb) and/or thyroid peroxidase (TPOAb). We performed a comprehensive phenotypic characterization of patients with HT, with specific focus on thyroid autoimmunity, to get better understanding of disease manifestation. METHODS: We collected information on thyroid-specific phenotypes (TSH, T3, T4, fT4, TgAb, TPOAb, thyroid volume) and other clinical phenotypes (age, body surface area, number of hypothyroidism symptoms, blood pressure) from 290 patients with HT without levothyroxine (LT4) therapy with the aim to test for correlations between thyroid-specific and clinical phenotypes...
October 17, 2018: Immunological Investigations
YunLong Zhuang, XiXi Li, Xiaohua Li, HuiCong Xu, Hui Ye, Di Sun, XiangZhong Liu, GuiJie Ren
BACKGROUND: A large proportion of patients with chronic hepatitis B (CHB) in China do not respond to entecavir (ETV) treatment. It remains unclear whether the Killer immunoglobulin-like receptor (KIR) genotypes and haplotypes were associated with the advantage of seroconversion in phepatitis B e-Antigen (HBeAg) positive CHB patients treated with ETV. METHODS: Polymerase chain reaction with sequence-specific primers (PCR-SSP) was used to analyze KIR genes in a Chinese Han population of 198 ETV-treated HBeAg-positive CHB patients and 200 healthy controls...
October 16, 2018: Immunological Investigations
Binnian Zhu, Tianyu He, Xiangyun Gao, Minghua Shi, Hongxiang Sun
PURPOSE: The purified fraction of Albizia julibrissin saponins (AJSAF) was evaluated and characterized for the adjuvant activity on porcine reproductive and respiratory syndrome virus (PRRSV) vaccine. METHODS: The effects of AJSAF on serum PRRSV N protein-specific antibody titers, splenocyte proliferation, natural killer (NK) cell activity, mRNA expression of cytokines and transcription factors, secretion of cytokines, T cells response in splenocytes, as well as delayed type hypersensitivity (DTH) in the mice immunized PRRSV vaccine were determined by ELISA, MTT assay, flow cytometry and quantitative real-time PCR (qRT-PCR)...
October 16, 2018: Immunological Investigations
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