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Journal of Basic Microbiology

Mariana Solans, María I Messuti, Gabriela Reiner, Micaela Boenel, Gernot Vobis, Luis G Wall, Jose M Scervino
This study tested the solubilization of phosphorus by five actinobacterial strains in liquid media containing Ca3 PO4 ; AlPO4 or FePO4 as the sole phosphate source, and discusses the possible mechanisms involved in this process. P solubilization by different strains was accompanied by a significant drop in pH from 7.0 to 2.15-5.0 after 14 days. The efficiency of different strains depended on the P-source. Streptomyces spp. MM140 and MM141 were the most efficient in solubilizing Ca3 PO4 , MM136, and MM141 were the most efficient in solubilizing AlPO4 , while all strains were equally efficient in solubilizing FePO4 ...
February 15, 2019: Journal of Basic Microbiology
Yuming Zhang, Chunhai Zhao, Zhihua Ni, Menghua Shao, Mengying Han, Dawei Huang, Fengsong Liu
Xylulose kinase is an important enzyme involved in xylose metabolism, which is considered as essential biocatalyst for sustainable lignocellulosic-derived pentose utilization. Bacillus coagulans IPE22 is an ideal bacterium for refinery due to its strong ability to ferment xylose at high temperature. However, the B. coagulans xylose utilization mechanism remains unclear and the related promising enzymes need to be developed. In the present study, the gene coding for xylulose kinase from B. coagulans IPE22 (Bc-XK) was expressed in Escherichia coli BL21 (DE3)...
February 12, 2019: Journal of Basic Microbiology
Zhou Chen, Yangliu Liu, Ahmed A Zaky, Lu Liu, Yaoyao Chen, Siting Li, Yingmin Jia
A novel xylanase from the filamentous fungus Aspergillus flavus was purified and characterized as the β-1, 4-endoxylanase (designed as AfXynB) with a molecular mass (32.2 kDa), which is different from all of the previously reported xylanases from the same strain. AfXynB was optimally active at pH 7.5 and 55 °C, respectively. It was stable up to 50 °C within range of pH 4.0-9.5, and displayed an excellent tolerance to various cations, reagents, and proteases. AfXynB showed specific activity toward beechwood xylan but no detected activity toward CMC and pNP-β-D-xylopyranoside...
February 12, 2019: Journal of Basic Microbiology
Egemen Özkan, Burcu Kartal, Merve Yılmazer, Bedia Palabıyık
This study focuses on the effect of iron on hexose transporters which perform glucose uptake. For this aim, we investigated the role of iron in glucose utilization and expression of hexose transporters in Schizosaccharomyces pombe. We applied different iron concentrations (1, 2, 5, 10 mM) to the cells grown up to mid-logarithmic phase. According to analysis of cell viability and morphology, we determined 2 mM and 5 mM as non-toxic and toxic doses, respectively. Besides, glucose consumption efficiency increased (1...
February 7, 2019: Journal of Basic Microbiology
Ola A Olapade, Lauren Rasmussen
The effects of varying concentrations (ranging from 0 to 10 μM) of two different metals that is, iron (Fe) and copper (Cu) on indigenous bacterial populations and their hydrolytic enzyme activities within the bacterioplankton assemblages from the surface waters of the Kalamazoo River were examined under controlled microcosm conditions. The two metals were added to water samples collected from the Kalamazoo River and examined for bacterial abundance and leucine aminopeptidase activities at various time intervals over a 48 h incubation period in the dark...
February 1, 2019: Journal of Basic Microbiology
Yalan Liu, Yiling Deng, Ping Chen, Mengjie Duan, Xiaoshan Lin, Yi Zhang
To discuss the microbial structure that develops during the course of compost burials of polyvinyl alcohol (PVA)-based material and analyze the effect of the degree of PVA alcoholysis on the PVA-degrading ability of each strain. Two bacterial strains exhibiting a high level of PVA-degrading ability and two mixed cultures were isolated from compost loaded with PVA-based material. The rod-shaped Gram-positive bacterium DG22 was identified by 16S rDNA sequencing analysis as Bacillus sp. DG22, and the rod-shaped Gram-negative bacterium DG14 was identified as Paenibacillus sp...
January 29, 2019: Journal of Basic Microbiology
Lynette Lincoln, Veena S More, Sunil S More
In the present study, glucoamylase produced from a soil bacterium Paenibacillus amylolyticus NEO03 was cultured under submerged fermentation conditions. The extracellular enzyme was purified by starch adsorption chromatography and further by gel filtration, with 2.73-fold and recovery of 40.02%. The protein exhibited molecular mass of ∼66,000 Da as estimated by SDS-PAGE and depicted to be a monomer. The enzyme demonstrated optimum activity at pH range 6.0-7.0 and temperature range 30-40 °C. Glucoamylase was mostly activated by Mn2+ metal ions and depicted no dependency on Ca2+ ions...
January 25, 2019: Journal of Basic Microbiology
Li Li, Wu Qu, Min Jin, Wenjie Di, Runying Zeng
An agarase gene (agaM1) was cloned, expressed and characterized by using Escherichia coli as host strain, revealing the outstanding properties of recombinant AgaM1 (rAgaM1) in agarose degradation and neoagaro-oligosaccharides (NAs) production in our previous work. In current study, agaM1 was extracellularly expressed in Bacillus subtilis, and we aim to assess the ability of the supernatant of recombinant B. subtilis fermentation broth containing rAgaM1 to degrade agarose without protein purification, which would save the cost of purification and avoid the activity loss during purification...
January 23, 2019: Journal of Basic Microbiology
Alok K Sinha, Bhaskar Parli Venkateswaran, Sarat C Tripathy, Amit Sarkar, Sabu Prabhakaran
Iron is an important element for growth and metabolism of all marine organisms, including bacteria. Most (99.9%) of iron in oceans are bound to organic ligands like siderophores and siderophore-like compounds. Distribution of bioavailable iron mainly depends on pH and temperature of the ocean. Due to global warming and ocean acidification, bioavailability of iron may alter and in turn effect the response of marine bacteria. In this study, we investigated the effect of growth conditions like pH, temperature, and iron (III) concentrations on growth and siderophore production in selected heterotrophic bacteria isolated from waters around Kerguelen Islands (KW) and Prydz Bay (PB)...
January 23, 2019: Journal of Basic Microbiology
Jing Peng, Di Wu, Yue Liang, Lei Li, Yanbin Guo
Rahnella aquatilis HX2 was isolated from Beijing vineyard soil and used as a plant growth-promoting rhizobacterium in the field. Previous studies have shown that it has a broad in vitro antimicrobial spectrum and could inhibit a variety of plant pathogenic bacteria and fungi. In this study, a gene, acdS, encoding 1-aminocyclopropane-1-carboxylic acid-deaminase was disrupted by in-frame deletion in the HX2 strain. Compared to the wild-type, the acdS-mutant had higher rates of nitrogen fixation, reduced indole-3-acetic acid production, lowered efficacy as a biological control agent against the grape crown gall pathogen Agrobacterium vitis...
January 15, 2019: Journal of Basic Microbiology
Yattapu P Reddy, Ravindra K Yadav, Keshawanand Tripathi, Gerard Abraham
The mutant strain of the diazotrophic cyanobacterium Anabaena doliolum able to tolerate high temperature was isolated by induced mutation techniques using ethyl methane sulphonate. This mutant strain exhibited higher temperature tolerance than the wild type. The wild type was able tolerate temperature up to 40 °C whereas the mutant was able to grow at an elevated temperature of 48 °C. This mutant exhibited higher growth rate, heterocyst frequency, and nitrogen fixation. Mutant strains exhibited comparable levels of chlorophyll, phycocyanin, PS II activity, and O2 evolution as compared to unexposed control...
January 14, 2019: Journal of Basic Microbiology
Xiaomin Wang, Shanyun Wang, Guoshuai Shi, Weidong Wang, Guibing Zhu
Ammonia oxidation, mainly driven by ammonia-oxidizing archaea (AOA) and bacteria (AOB), plays an important role in determining the rate of nitrification in riparian zones. However, the underlying factors driving the distribution and activity of AOA and AOB in riparian zones, especially in the rhizosphere of Phragmites communis remain unknown. This study revealed the dominance of AOA in ammonium oxidization with higher abundance and activity in both rhizosphere and bulk soil in summer and winter over AOB in riparian zones, based on molecular methods and double-inhibitors method...
January 9, 2019: Journal of Basic Microbiology
Can Yin, Yunbo Wang, Yongan Zhang, Haihua Wang, Ran Tao, Yu Li, Chang Keun Sung
Esteya vermicola is an endophytic fungus of pine wood nematode with demonstrated biocontrol efficacy. At present, the methods for detection of this fungus from pine wood is still inconvenient and inefficient such as thin-section microscopic observations. In the present study, a simple protocol was developed for wood sample preparation for effective extraction of fungal DNA from wood samples for PCR detection. The protocol of preparation of the sample involves washing in sterile water overnight on a shaking table followed by filtration and centrifugation to obtain the extracted DNA...
January 9, 2019: Journal of Basic Microbiology
Shilpa Mujumdar, Prachi Joshi, Neha Karve
Bioemulsifiers (BE) and biosurfactants (BS) are considered as multifunctional biomolecules of 21st century because of their functional abilities and eco-friendly properties. They are produced by various microorganisms under versatile and extreme environmental conditions. They have tremendous applications in various industries such as petroleum, food, medicine, pharmaceutical, chemical, paper & pulp, textile, and cosmetics. Currently, they are also considered as "green molecules" because of their wide applications in bioremediation of soil...
January 7, 2019: Journal of Basic Microbiology
Nagarajan Kumaran, M Hussain Munavar
Δlon mutant of Escherichia coli becomes hypersensitive to DNA damaging agents and over-produce capsule due to stabilization of the Lon substrates, namely, SulA and RcsA, respectively. These phenotypes were earlier found to be suppressed in Δlon ssrA::cat/pUC4 K and Δlon faa (DnaJ, G232D) strains, called as "Alp" strains. We observed that a plasmid carrying an E. coli chromosomal fragment harboring few genes, a heat shock gene htpY and a portion of dnaK capable of encoding truncated N-terminal ATPase domain (244 aa) could suppress lon mutant phenotypes...
January 7, 2019: Journal of Basic Microbiology
John Selesteen Charles Adlin Jenifer, Mariavincent Michaelbabu, Chinnadurai Lelin Eswaramoorthy Thirumalaikumar, Selva Raj Jeraldin Nisha, Ganapathi Uma, Thavasimuthu Citarasu
Antagonistic haloalkaliphilic Nocardiopsis sp. AJ1 (GenBank JX575136.1), isolated and identified from the saline soil of Kovalam solar salterns was able to produce antimicrobial secondary metabolites and effectively suppressed several bacterial and fungal pathogens. The metabolite extracted from ethyl acetate precipitation suppressed the bacterial and fungal pathogens to the range between 2.14 and 20.14 mm and also controlled the shrimp killer virus WSSV by 83% than the control and significantly (p < 0...
January 3, 2019: Journal of Basic Microbiology
Saurav Haloi, Tapas Medhi
A biosurfactant, produced by Achromobacter sp. TMB1 is reported through this investigation having physiochemical properties useful for operational and remedial activities in petroleum industries. The strain isolated from soils of local petrol pumps when allowed to grow in glucose containing mineral salts media (MSM) in ambient environment that is, 30 °C and varying pH in between 5.5 and 7.2, showed surface tension reduction as low as 34.25 dyne cm-1 at an interval of 48-72 h. Taguchi experiment confirmed the contribution of glucose, yeast extracts, and NaNO3 concentration on biosurfactant production...
January 2, 2019: Journal of Basic Microbiology
Wasim Sajjad, Guodong Zheng, Xiangxian Ma, Muhammad Rafiq, Muhammad Irfan, Wang Xu, Barkat Ali
The exploration of microbial diversity in extremely acidic habitats has provided a vital base for the progression of minerals biotechnology. Three indigenous iron-oxidizing acidophilic bacterial strains were isolated through serial dilution of enriched bacterial culture from Baiyin Copper Mine Stope, China. The morphological, biochemical, physiological, and phylogenetic characteristics of isolates were investigated. These isolates were motile, Gram-negative, and curved shape with pleomorphism except isolate WG101 that was a straight rod...
December 28, 2018: Journal of Basic Microbiology
Hongyu Zhang, Shirui Dong, Tingting Lou, Suying Wang
Marine Streptomyces sp. CC0208 isolated from the Bohai Bay showed high efficiency of cellulose degradation under optimized fermentation parameters. Also, as one of the bioinformatics-based approaches for the discovery of novel natural product and enzyme effectively, genome mining has been developed and applied widely. Herein, we reported the complete genome sequence of Streptomyces sp. CC0208.Whole-genome sequencing analysis revealed a genome size of 9,325,981 bp with a linear chromosome, GC content of 70...
December 27, 2018: Journal of Basic Microbiology
BalaAbirami Sundaramoorthy, Sathyanarayana N Gummadi
Arabitol has several applications in food and pharmaceutical industries as a natural sweetener, dental caries inhibitor, and texturing agent. Newly isolated yeast strains from seawater, sugarcane plantation soil samples, and Zygosaccharomyces rouxii 2635 from MTCC were tested for arabitol production. The yield of arabitol was found to be higher in seawater isolate (24.6 g L-1 ) compared to two soil isolates (22.5 g L-1 ) and Z. rouxii (19.4 g L-1 ). Based on ITS 26S rDNA sequence analysis, the seawater isolate was identified as Pichia manchurica...
December 27, 2018: Journal of Basic Microbiology
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