Journals Folia Histochemica et Cytobiol...

Folia Histochemica et Cytobiologica
Guangbo Xiang, Kai Shi, Jinjun Wang
INTRODUCTION: Lupus nephritis (LN) is an autoimmune glomerulonephritis secondary to systemic lupus erythematosus. Commonly, immunosuppressive agents are required for treating LN. However, frequent use of conventional immunosuppressants may produce a variety of side effects. Hence, seeking alternative drugs for treating LN is very important. This report aims to figure out the immunoregulatory efficacy of celastrol (CLT) in LN. MATERIAL AND METHODS: A spontaneous in vivo model of LN was established in FasL-deficient B6/gld mice...
2022: Folia Histochemica et Cytobiologica
Ming Yu, Zhengguo Yi, Shenghui Chen, Xiaopeng Chen, Xiaofeng Xie
INTRODUCTION: The purpose of this study is to elucidate the impact of long non-coding RNA (lncRNA) MIR4435-2HG/microRNA (miR)-125b-5p/ Semaphorins 4D (Sema4D) on colorectal cancer (CRC) cell propagation and migration. MATERIAL AND METHODS: Sema4D expression in 73 pairs of CRC tissues and matched adjacent normal tissues was measured by qRT-PCR and western blot and its association with pathological characteristics of CRC patients was analyzed by chi-square test. Also, the expression of MIR4435-2HG, miR-125b-5p and Sema4D in CRC cell lines was detected by qRT-PCR and Western blot...
2022: Folia Histochemica et Cytobiologica
Qian Zhang, Yan Cui, Sijiu Yu, Junfeng He, Meng Wang, Yangyang Pan, Kun Yang, Kun Yang
INTRODUCTION: The thymus is the site of development and maturation of functional T lymphocytes and is critically important to the immune system. The purpose of this study was to examine the expression of markers of T lymphocytes, macrophages, dendritic cells, B lymphocytes and plasmocytes in the yak thymus. MATERIALS AND METHODS: Twenty healthy male yaks were divided into newborn (2-4 weeks old, n = 10) and adult (3-4 years old, n = 10) group. qRT-PCR was used to evaluate the mRNA expression level of the main markers of the studied cell types...
2022: Folia Histochemica et Cytobiologica
Ying Liu, Yunen Liu, Yan Zhao, Mi Wu, Shun Mao, Peifang Cong, Rufei Zou, Mingxiao Hou, Hongxu Jin, Yongli Bao
INTRODUCTION: Clarifying the role and mechanism of exosome gel in wound repair can provide a new effective strategy for wound treatment. MATERIALS AND METHODS: The cellular responses of adipose mesenchymal stem cell-derived exosomes (AMSC-exos) and the wound healing ability of AMSC-exos-loaded β-chitin nanofiber (β-ChNF) hydrogel were studied in vitro in mouse fibroblasts cells (L929) and in vivo in rat skin injury model. The transcriptome and proteome of rat skin were studied with the use of sequenator and LC-MS/MS, respectively...
2022: Folia Histochemica et Cytobiologica
Fouzia Zerrouk, Billel Chaouad, Adel Ghoul, Naima Chalour, Anissa Moulahoum, Zineb Khiari, Mohamed El Hadi Cherifi, Souhila Aouichat, Karim Houali, Yasmina Benazzoug
INTRODUCTION: Cardiovascular diseases were defined as coronary artery, cerebrovascular, or peripheral arterial disease. Hyperhomocysteinemia (Hhcy) is an independent risk factor of cardiovascular diseases, including atherosclerosis. Our previous studies demonstrated the involvement of Hhcy in cardiovascular remodeling in the sand rat Psammomys obesus. MATERIAL AND METHODS: An experimental Hhcy was induced, in the sand rat Psammomys obesus, by a daily intraperitoneal injection of 70 mg/kg of methionine for a total duration of 6 months...
2022: Folia Histochemica et Cytobiologica
Xia Yi, Ping Wu, Yunyan Fan, Ying Gong, Jianyun Liu, Jianjun Xiong, Xiaoyuan Xu
INTRODUCTION: In osteoporosis field, it had been clinically well established a given relationship between bone formation and lipid accumulation. Although numerous molecules had been well documented for adipogenesis and osteoblastogenesis (adipo-osteoblastogenesis), the reciprocal transcriptional regulation still remains to be explored. MATERIAL AND METHODS: Here, we tried to identify the common candidate genes of adipocyte/osteoblastocyte differentiation at 3, 5, and 7 days using human mesenchymal stem cells (hMSCs) via RNA-Seq technique...
2022: Folia Histochemica et Cytobiologica
Xiao-Ning Lu, Guan-Jun Ju, Yu-Xin Wang, Yong-Liang Wang, Kun Wang, Jian-Le Chen, Wei Cai, Qi-Wei Zang
INTRODUCTION: To investigate the clinical significance of Ly-1 antibody reactive clone (LYAR) in non-small-cell lung cancer (NSCLC). MATERIAL AND METHODS: The expressions of LYAR at the protein level in representative paired NSCLC tumor tissues and adjacent non-tumor tissues were measured by Western blot and immunohistochemistry. Kaplan-Meier method was used to calculate the survival curve of patients with NSCLC. Cell Counting Kit-8 assay and flow cytometry were used to estimate the cell proliferation and cell cycle, respectively...
December 10, 2021: Folia Histochemica et Cytobiologica
Natalia Matysiak, Lukasz Mielanczyk, Krzysztof Kaczmarek, Malgorzata Zaba, Edyta Reichman-Warmusz, Romuald Wojnicz
INTRODUCTION: Cardiac papillary fibroelastomas (CPFs) are rare benign cardiac tumors typically found on the heart valves. The previously published data on the CPF focused on its clinical presentation, optimal management, and prognosis. However, histogenesis of these lesions remains controversial. Accordingly, the aim of this study was to establish the role of endocardial endothelium (EE) in CPF formation. MATERIALS AND METHODS: Four CPF tumors removed from the right atrioventricular valves were analyzed using hematoxylin & eosin, orcein, and Masson trichrome staining together with immunochemistry for CD-34, CD-68, vimentin, vWF and α-SMA...
December 8, 2021: Folia Histochemica et Cytobiologica
Li Yun, Lining Wang, Yalan Pan, Mengmin Liu, Qiuge Han, Jie Sun, Guanglu Yang, Jirimutu Xiao, Yang Guo, Yong Ma
INTRODUCTION: . The occurrence of osteoporosis (OP) has drawn considerable attention from scholars around the world due to the significant impacts thereof on the social economy and the quality of human life. OP research has been rapidly expanding since the inclusion of microRNAs (miRNAs) as critical regulators of gene-expression. However, despite the ability to evaluate miRNA gene therapy in OP being enhanced, there has been a scarcity of updated citation analyses that reflect such developments...
December 1, 2021: Folia Histochemica et Cytobiologica
Yan Liu, Kai Zhao, Zhe Xiong, Yuanxiao Yi, Chengliang Xiong, Donghui Huang, Hu Zhao
INTRODUCTION: Urokinase plasminogen activator (uPA) is a serine protease and it also demonstrates proinflammatory properties. We thus hypothesized that uPA is involved in immunity of Sertoli cells in the rat testis. MATERIALS AND METHODS: The uPA gene(PLAU) promoter was cloned by RT-PCR and the transcriptional activation of core domain was screened by using Dual-Luciferase Reporter Assay System. The Sertoli cells were harvested from 20-day-old Sprague-Dawley male rats and total RNA was isolated...
December 1, 2021: Folia Histochemica et Cytobiologica
Jingyi Zeng, Xiaorong Bao
INTRODUCTION: The progression of diabetic kidney disease (DKD) is closely related to renal tubular epithelial-to-mesenchymal transition (EMT) and tubulointerstitial fibrosis. Tanshinone IIA (TSIIA), extracted from a traditional Chinese medicine named Salvia miltiorrhiza, has been proved to have anti-fibrosis effects. The aim of this study was to investigate the effect of TSIIA on high glucose-induced EMT in human proximal tubular cells (HK-2 cells) and its possible mechanism. MATERIAL AND METHODS: The proliferation of cells exposed to different concentrations of glucose was measured by light microscopy and CCK-8 test...
December 1, 2021: Folia Histochemica et Cytobiologica
Chunchun Gan, Zhan Jin, Xiaopeng Wei, Meina Jin
INTRODUCTION: Actinidia chinensis Planch. root extract (acRoots), known as a traditional Chinese medicine (TCM), has shown antitumor efficacy in various types of human cancers. However, its role and underlying mechanisms in breast cancer (BCa) have not been elucidated. MATERIAL AND METHODS: In the present study, the effects of acRoots on cell viability, apoptosis, migration and invasion were analyzed by MTT assay, colony formation, flow cytometry, wound healing and Transwell assay in MDA-MB-231 and MDA-MB-453 breast cancer cell lines...
December 1, 2021: Folia Histochemica et Cytobiologica
Xiaoyan Yu, Yifei Jiang, Xun Hu, Xiang Ge
INTRODUCTION: Long noncoding RNAs are associated with progressions of lung cancer. LINC00839 has been dysregulated in osteosarcoma, breast cancer and lung cancer (LC). As an upregulated lncRNA, the roles of LINC00839 in lung cancer remain unclear. MATERIAL AND METHODS: RNA expressions of LINC00839, miR-519d-3p and JMJD6 were assessed using RT-qPCR and JMJD6 protein expressions were analyzed through Western blot. Meanwhile, viabilities of A549 and H460 LC cells transfected by siNC, siLINC00839, oeNC, oeLINC00839, NC mimics, miR-519d-3p mimics and oeLINC00839 with siJMJD6 were examined with CCK-8 assay while apoptosis was examined using flow cytometry...
November 4, 2021: Folia Histochemica et Cytobiologica
Wen Xiong, Xin Guo, Xianhua Cai
MATERIAL AND METHODS: BMSCs were treated with 100 μg/L SDF-1 and cultured in osteogenic medium for 7 days. RT-qPCR and western blotting were used to detect the protein and mRNA levels of Janus kinase 2 (JAK2), signal transducer and activator of transcription 3 (STAT3), Runt-related transcription factor 2 (Runx2), and osteocalcin (OCN). Alizarin-red staining was used to detect the mineralization-inducing ability of the cells. RESULTS: . After BMSCs were treated with SDF-1, the levels of JAK2 mRNA, STAT3 mRNA, and protein phosphorylation increased, the number of mineralized nodules of BMSCs increased, and the osteogenic-differentiation ability was enhanced...
September 28, 2021: Folia Histochemica et Cytobiologica
Wei Xiong, Hua Chen, Jiequan Lu, Jie Ren, Chen Nie, Ruijuan Liang, Feng Liu, Baofeng Huang, Yu Luo
INTRODUCTION: The cytokine interleukin (IL)-39 is a novel member of the IL-12 family. Our previous study found that the serum level of IL-39 significantly increased in patients with acute myocardial infarction. However, the role of IL-39 in cardiomyocyte apoptosis remains unclear. MATERIAL AND METHODS: In this study, the cultured mouse HL-1 cardiomyocytes were incubated with PBS, 0-100 ng/mL IL-39, 200 μM H₂O₂ or 20 μM Trolox. RESULTS: IL-39 promoted the production of intracellular reactive oxygen species (ROS) in a concentration-dependent manner in HL-1 cardiomyocytes...
September 20, 2021: Folia Histochemica et Cytobiologica
Adrian Zubrzycki, Agata Wronska, Agata Zauszkiewicz-Pawlak, Zbigniew Kmiec
INTRODUCTION: Fenofibrate (FN) is a hypolipemic drug used for the treatment of mixed dyslipidemia. Since in our previous study FN administration to young and old rats adversely affected the serum activity of liver marker enzymes, we decided to examine the effects of FN on liver ultrastructure of young and old animals. MATERIAL AND METHODS: Young and old rats were fed standard rodent chow supplemented with 0.1% FN for 30 days. Liver samples obtained from animals under full anesthesia were processed by routine methods to obtain ultrathin and histological sections for the examination by light microscopy (LM) and transmission electron microscopy (TEM)...
September 13, 2021: Folia Histochemica et Cytobiologica
Pablo Sánchez, Manuel Lisardo Sánchez, Arturo Mangas, Eliana de Souza, Luis Angel Aguilar, Rafael Coveñas
INTRODUCTION: A recent study has shown a close neuroanatomical relationship between the enkephalinergic (methionine-enkephalin) and tachykininergic (substance P) systems in the alpaca diencephalon. In this study, our aim is to show this relationship in the alpaca brainstem. MATERIAL AND METHODS: Using an immunohistochemical technique, the distribution of immunoreactive (Ir) fibers and cell bodies containing substance P (SP) or methionine-enkephalin (MET) has been studied in the alpaca brainstem...
July 26, 2021: Folia Histochemica et Cytobiologica
Kelan Zha, Qiang Ye
INTRODUCTION: Vascular smooth muscle cells (VSMCs)-based foam cell formation is a crucial factor in the atherosclerosis process. We aimed to explore the mechanism of Golgi α-mannosidase II (GMII) effects on the VSMCs-based foam cell formation. MATERIAL AND METHODS: VSMCs were exposed to different concentrations of low-density lipoproteins (LDLs), lipopolysaccharide (LPS), and/or GMII inhibitor (swainsonine). The qRT-PCR and western blot were used for expression analysis...
June 21, 2021: Folia Histochemica et Cytobiologica
Agnieszka Piekarska, Michaël Pérès, Magdalena Toton, Malgorzata Kulczycka, Krzysztof Lewandowski, François Vergez
INTRODUCTION: Regulatory T cells (Tregs) are a unique CD4+ T cell subset involved in the regulation of immune responses. The traditional immunophenotype used to define Tregs includes CD4+CD25high and the expression of the transcription factor Forkhead box protein 3 (FoxP3). A complex technique of intracellular staining, transient upregulation of FoxP3 in activated conventional T lymphocytes (Tcons), and the omission of naïve CD45RA+ Tregs with downregulated FoxP3 activity but a demethylated FOXP3 promoter region may lead to inaccurate quantification...
June 7, 2021: Folia Histochemica et Cytobiologica
Edyta Reichman-Warmusz, Marlena Brzozowa-Zasada, Celina Wojciechowska, Damian Dudek, Oliwia Warmusz, Romuald Wojnicz
INTRODUCTION: Endothelial dysfunction is a critical part of heart failure (HF) pathophysiology. It is not clear, however, whether it is present at the similar level in the early and late HF stages. MATERIAL AND METHODS: von Willebrand factor (vWF) and its mRNA levels in biopsies of non-ischemic patients with HF secondary to dilated cardiomyopathy were studied. Consecutive patients with HF were divided into two groups: group A with disease duration ≤ 12 months (n = 59) and group B with disease duration > 12 months (n = 68)...
May 18, 2021: Folia Histochemica et Cytobiologica
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read

Save your favorite articles in one place with a free QxMD account.


Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.