Journals Folia Histochemica et Cytobiol...

Folia Histochemica et Cytobiologica
Predaporn Nonsiri, Potchara Srivilai, Supatsorn Onkaew, Nontawat Benjakul
INTRODUCTION: Losing of small tissues during tissue preparatory steps may seriously affect pathological diagnostic performance. Using an appropriate tissue marking dye could be an alternative solution. Therefore, the aim of the study was to find a suitable tissue marking dye to enhance the observable ability of various types of small-size tissues during several steps of tissue preparation. MATERIAL AND METHODS: Various small-size samples of various organs and tissues (0...
2023: Folia Histochemica et Cytobiologica
Jiahui Han, Di Jia, Hao Yao, Ting Lv, Xi Xu, Xin Ge
INTRODUCTION: Hemorrhagic shock (HS) is an important cause of high mortality in traumatized patients. Cryptotanshinone (CTS) is a bioactive compound extracted from Salvia miltiorrhiza Bunge (Danshen). The current study aimed to explore the effect and underlying mechanism of CTS on the liver injury induced by HS. MATERIAL AND METHODS: Male Sprague-Dawley rats were used to establish the HS model by hemorrhaging and monitoring mean arterial pressure (MAP). CTS was intravenously administered at concentration of 3...
2023: Folia Histochemica et Cytobiologica
Tian Xie, Ruijie Pan, Wenzhuo Huang, Sheng Dong, Shizhen Wu, Yuhui Ye
INTRODUCTION: Transplantation of mesenchymal stem cells (MSCs) has been reported to be a novel promising target for the regeneration of degenerated intervertebral discs (IVDs). However, the culture and survival limitations of MSCs remain challenging for MSC-based biological therapy. Myricetin, a common natural flavonoid, has been suggested to possess antiaging and antioxidant abilities. Therefore, we investigated the biological function of myricetin, and its related mechanisms involving cell senescence in intervertebral disc degeneration (IDD)...
2023: Folia Histochemica et Cytobiologica
Jae-Rim Son, Xin-Yu Kuai, Chang-Jin Jeon
INTRODUCTION: While most animals of the Muridae family are nocturnal, the gerbil displays diurnal activity and provides a useful model for visual system research. The purpose of this study was to investigate the localization of calcium-binding proteins (CBPs) in the visual cortex of the Mongolian gerbil (Meriones unguiculatus). We also compared the labeling of CBPs to those of gamma-aminobutyric acid (GABA)- and nitric oxide synthase (NOS)-containing neurons. MATERIAL AND METHODS: The study was conducted on twelve adult Mongolian gerbils (3-4 months old)...
2023: Folia Histochemica et Cytobiologica
Qian Zhang, Yan Cui, Sijiu Yu, Junfeng He, Yangyang Pan, Meng Wang, Gengquan Xu
INTRODUCTION: As a main consumer of energy, the brain is particularly susceptible to the effects of hypoxia. However, during long-term evolution, the brain of the plateau yak developed adaptive mechanisms enabling it to maintain normal physiological conditions. MATERIAL AND METHODS: A total of 20 male yaks belonging to two age groups [newborns (1-6 days old; n = 10) and adults (3-5 years old; n = 10)] were obtained, and the brain tissue was fixed and processed by standard methods...
2023: Folia Histochemica et Cytobiologica
Lili Ji, Xiaojing Shi, Gaopin Wang, Huiping Wu, Zhansheng Hu
INTRODUCTION: Acute lung injury (ALI) is a major cause of death in sepsis patients. The Six-transmembrane protein of prostate 2 (STAMP2) is a key regulator of inflammation, while its role in septic ALI remains unclear. MATERIAL AND METHODS: Male C57BL/6 mice were subjected to cecal ligation puncture (CLP) to induce experimental sepsis whereas lipopolysaccharide (LPS)-stimulated RAW 264.7 cells were used as the models of septic ALI in vivo and in vitro, respectively...
December 30, 2022: Folia Histochemica et Cytobiologica
Wu Xiong, Xin-Ling Huang, Xiao-Liang Wang, Hong-Wei Lan, Ting-Ting Wang, Zi-Lin Chen, Qian-Pei Yang, Ai-Lin Hu, Yi-Fei Xia, Zhong-Zhi Zhou
INTRODUCTION: As one of the basic components of Astragalus, Astragaloside IV (AS-IV) has a protective effect on endothelial injury caused by diabetes. AS-IV stimulated endothelial progenitor cells (EPCs) to secrete exosomes loaded with miR-21. This study aimed to investigate the effects of AS-IV-mediated EPCs exosomal miR-21 (EPC-exos-miR-21) on high glucose (HG) damaged endothelial cells. MATERIALS AND METHODS: After the isolation of EPCs derived from fetal umbilical cord blood, exosomes of EPCs were obtained by differential centrifugation...
December 12, 2022: Folia Histochemica et Cytobiologica
Xiaojin Ye, Huailiang Xu, Yuan Xu
INTRODUCTION: Ulcerative colitis (UC) is a nonspecific intestinal inflammatory disease. Dexmedetomidine (DEX) is a selective alpha 2-adrenergic receptor agonist commonly used for analgesia and sedation in intensive care units. Herein, the role and mechanism of DEX in dextran sulfate sodium (DSS)-induced colitis was explored. MATERIALS AND METHODS: A murine model of DSS-induced colitis was established by adding 3.5% (w/v) DSS in drinking water to C57BL/6J female mice...
December 12, 2022: Folia Histochemica et Cytobiologica
Jingjing Cao, Yanhui Ni, Xiaoran Ning, Huaxing Zhang
INTRODUCTION: Rheumatoid arthritis (RA) is an autoimmune disorder associated with joint damage and attendant cardiovascular complications. Wedelolactone (Wed), derived from Eclipta alba, possesses anti-inflammatory activity. Whether Wed regulates RA inflammation and related heart damage remains unknown. MATERIAL AND METHODS: A murine model of collagen-induced arthritis (CIA) was well-established by two subcutaneous injections of type II collagen (days 0 and 21)...
November 16, 2022: Folia Histochemica et Cytobiologica
Xingnuan Li, Shan He, Ping Wu, Yichun Zhou, Kai Long, Tao Wang
INTRODUCTION: Adipogenesis, a highly coordinated process regulated by numerous effectors, is largely responsible for the quantity and size of adipocytes. Attenuation of adipocyte differentiation has been proposed as a viable technique for reducing obesity and its associated diseases. microRNAs play an important role in human bone marrow mesenchymal stem cells (hMSCs) adipogenic differentiation. However, there is a lack of clarity regarding the role of miR-328-5p in adipogenesis. MATERIAL AND METHODS: Using the lentiviral vectors to overexpress fatty acid synthase (FASN) and miR-328-5p, RT-qPCR and Western blotting were carried out to assess RNA expression and protein levels of FASN and adipogenic marker factors...
October 27, 2022: Folia Histochemica et Cytobiologica
Ying Wang, Yu Wang, Yao Chen, Qianqian Gao, Lihui Hou, Xiaoling Feng
INTRODUCTION: Abnormal ovarian angiogenesis is a common feature of polycystic ovary syndrome (PCOS), a typical endocrine disorder affecting women of reproductive age. Histone deacetylase 5 (HDAC5) has been documented as a suppressor of angiogenesis. The aim of this study was to explore the effect of HDAC5 on ovarian angiogenesis in a PCOS mouse model. MATERIAL AND METHODS: PCOS was induced in female C57BL/6 mice by 20-day administration of dehydroepiandrosterone (DHEA)...
September 20, 2022: Folia Histochemica et Cytobiologica
Katarzyna Błachnio, Beata Grygalewicz, Renata Woroniecka, Jolanta Rygier, Barbara Pieńkowska-Grela, Grzegorz Rymkiewicz, Jerzy Kawiak
INTRODUCTION: Standard treatment for chronic lymphocytic leukemia (CLL) has experienced a dramatic change over the last few years. Until recently, CLL was treated using chemoimmunotherapy (CIT) with anti-CD20 monoclonal antibodies. Even though novel agents such as BTKi (Bruton Tyrosine Kinase inhibitor) and BCL2 inhibitors are the standard of care in most therapeutic settings, CIT still has its place in CLL treatment. Interestingly, little is known about its effects on the immune system of patients with CLL...
September 7, 2022: Folia Histochemica et Cytobiologica
Fangfang Wang, Yanan Zhang, Jing Ren, Wencheng Yu
INTRODUCTION: The present study aimed to investigate the effect of homeodomain interacting protein kinase 2 (HIPK2) on pulmonary fibrosis and the probable mechanisms. MATERIAL AND METHODS: We constructed a mouse model of bleomycin-induced pulmonary fibrosis and up-regulated the expression of HIPK2 in the lung by in vivo transfection. Lung tissues were collected for the detection of mesenchymal markers (α-SMA, collagen I, collagen III, etc.) and the expression of β-catenin by RT-PCR, western blot, and immunohistochemistry...
August 25, 2022: Folia Histochemica et Cytobiologica
Hui Shi, Xiaoyu Liu, Yanwei Wang, Haiyan Wang, Bochen Pan, Jianyuan Li
INTRODUCTION: Peroxiredoxin 6 (Prdx6) is widely expressed in mammalian tissues. Our previous study demonstrated that Prdx6 was expressed in human epididymis, present in human seminal fluid, and in spermatozoa. The protective role of Prdx6 in maintaining the viability and DNA integrity of human spermatozoa was also detected. Here, we demonstrate the potential role and mechanism of Prdx6 in human epididymis epithelial cells (HEECs). MATERIAL AND METHODS: Western blotting was used to measure expression levels of key proteins in the Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway...
August 5, 2022: Folia Histochemica et Cytobiologica
Yaning Wei, Qian Zhang, Lin An, Guotao Fang, Dan Hong, Ting Jiao, Hua Yang, Zhiyu Wang
INTRODUCTION: Evidence has shown that some microRNAs (miRNAs) play a role in tumorigenesis of hepatocellular carcinoma (HCC). Herein, we aimed to evaluate the diagnostic and prognostic values of serum exosomal miR-370-3p and miR-196a-5p in patients with HCC. MATERIAL AND METHODS: Serum exosomes in 90 HCC patients were extracted and identified. Serum exosomal miR-370-3p and miR-196a-5p expression in HCC patients were detected. The diagnostic value of miR-370-3p and miR-196a-5p, relationship between miR-370-3p and miR-196a-5p expression and clinicopathological features and prognosis of patients with HCC were analyzed...
June 28, 2022: Folia Histochemica et Cytobiologica
Boonyarit Kamkrathok, Yupaporn Chaiseha
INTRODUCTION: . Neuropeptide Y (NPY), a 36 amino acid neurotransmitter/neuromodulator, is involved in food intake and parental cares in birds. NPY is associated with the regulation of the reproductive system in the female native Thai chickens. However, the role of NPY in the male native Thai chicken has not been studied. Therefore, the objective of this study was to investigate the distributions of NPY immunoreactive (-ir) neurons and fibers in the brain of the male native Thai chickens...
June 6, 2022: Folia Histochemica et Cytobiologica
Yihai Lin, Liang Guo
INTRODUCTION: Glioma is characterized by hypoxia that activates the hypoxia inducible factor (HIF) pathway and controls a myriad of genes that drive cancer progression. HIF-1α promotes GLI1 transferring to the nucleus by activating the hedgehog pathway under hypoxic conditions. However, their mechanisms in glioma cells under hypoxia remain unknown. MATERIAL AND METHODS: Human glioma cell lines (LN229 and LN18) were transfected with HIF-1α or GLI1-specific short hairpin RNAs (shRNAs) and cultured under normoxic or hypoxic conditions...
May 23, 2022: Folia Histochemica et Cytobiologica
Dingchun Li, Dehong Ma, Ye Liu, Lihui Liu, Yihui Chen, Huaie Liu, Lu Zhang, Jie Lu, Kexuan Chen, Wu Li, Jing You
INTRODUCTION: Liver fibrosis is caused by continuous wound healing responses to various harmful stimuli, including viral infection, drugs, alcohol, and autoimmune liver disease. The purpose of this study was to examine the effects of extracts of Periplaneta americana (EPA) in rats with pig serum-induced liver fibrosis to preliminarily assess the antifibrotic effect of EPA. MATERIAL AND METHODS: Seventy rats were randomly divided into 7 groups (10 rats in each group): HC, the healthy control group; FC, the fibrotic control group; TL, low-dose EPA treatment group group; TM, medium-dose EPA group; TH, high-dose EPA treatment group; TC1, Panax notoginseng/Salvia mitiorrhiza treatment control group 1; TC2, colchicine treatment control group 2...
May 16, 2022: Folia Histochemica et Cytobiologica
Shubo Li, Hongqin Tu
INTRODUCTION: . Psoralen is a main active component of Psoralea corylifolia Linn. (Leguminosae). Psoralen has been reported to show antitumor effects and activity to accelerate osteoblastic proliferation. Nevertheless, the antitumor mechanism of psoralen in osteosarcoma has never been elucidated. The current study is aimed to investigate the therapeutic function of psoralen in human osteosarcoma cells and its potential regulatory mechanism. MATERIAL AND METHODS: Effects of psoralen (0-70 μg/mL) on the viability of two osteosarcoma cell lines cultured for 48 h was evaluated by MTT assays...
March 8, 2022: Folia Histochemica et Cytobiologica
Xia Yi, Ping Wu, Ying Gong, Jianyun Liu, Jianjun Xiong, Xiangxin Che, Xiaoyuan Xu
INTRODUCTION: With cellular lipid storage varying, the balance between lipid intake and lipid degradation was a must to keep healthy and determined the level of lipid droplets. Although lipid droplets accumulation had been well demonstrated in adipocytes, gene expression profiling and gene function during adipogenesis and osteoblastogenesis remain unknown. MATERIAL AND METHODS: Here, this work profiled gene transcriptional landscapes of lipid droplets formation during adipogenesis from human mesenchymal stem cells (hMSCs) using RNA-Seq technique...
February 25, 2022: Folia Histochemica et Cytobiologica
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