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Folia Histochemica et Cytobiologica

Kamil Gill, Joanna Jakubik, Michal Kups, Aleksandra Rosiak-Gill, Rafal Kurzawa, Maciej Kurpisz, Monika Fraczek, Malgorzata Piasecka
INTRODUCTION: Contemporary professional jobs that often enforce a sedentary lifestyle and are often associated with testicular overheat, deserve special attention with respect to male fertility potential. Interestingly, the harmful effect of testicular heat stress on sperm characteristics including nuclear DNA integrity was well characterized; however, the influence of sedentary work on sperm chromatin has not yet been documented. Therefore, our research was designed to examine the potential effects of sedentary work not only on conventional semen features but also on sperm nuclear DNA status...
March 14, 2019: Folia Histochemica et Cytobiologica
Katarzyna Cierpka-Kmiec, Agata Wronska, Zbigniew Kmiec
Diabetes mellitus is a chronic disease that affects hundreds of millions of people worldwide. Type 1 diabetes (T1D) is characterized by the lack of pancreatic β-cells that had been destroyed as a result of an autoimmune response. Therefore, in patients with T1D, the replacement therapy with functional β-cells derived from extrinsic sources could be a preferable option as compared to insulin treatment. Unfortunately, successful transplantation of whole pancreata or pancreatic islets into patients with diabetes is available only to a fraction of them due to the scarcity of donors...
March 14, 2019: Folia Histochemica et Cytobiologica
Anna Gasinska, Janusz Jaszczynski, Agnieszka Adamczyk, Anna Janecka-Widła, Waclaw Wilk, Anna Cichocka, Andrzej Stelmach
INTRODUCTION: It has been suggested that the metastatic potential of neoplastic cells can be predicted on the basis of their biological features, including expression of proteins involved in the epithelial to mesenchymal transition (EMT). Therefore, the purpose of this work was to (1) evaluate the expression of EMT markers: ZEB2, vimentin, N-cadherin, TWIST, PTEN, survivin, E-cadherin, Ki-67 and GLUT-1, (2) assess mutation status of two genes: PIK3CA and KRAS, and (3) investigate the potential relationships between the studied biomarkers and clinicopathological factors in clear-cell renal cell carcinoma (ccRCC)...
December 20, 2018: Folia Histochemica et Cytobiologica
Justyna Sokolowska, Joanna Berczynska, Aleksandra Poweska, Dorota Rygiel, Katarzyna Olbrych, Kaja Urbanska
INTRODUCTION: C cells constitute a small percentage of thyroid gland parenchyma. The number, morphology and distribution of C cells differ among species; however, data regarding their characteristics in European bison are sparse. The aim of this study was to evaluate the morphology, distribution pattern and percentage of C cells in European bison thyroid gland together with morphometric analysis. MATERIAL AND METHODS: Thyroid glands from 28 European bisons of different sex and age were collected either in autumn-winter (13/28) or in spring-summer (15/28) periods and analyzed by immunohistochemistry...
December 19, 2018: Folia Histochemica et Cytobiologica
Aziza Alrafiah, Maimonah Alghanmi, Sufana Almashhadi, Aqeel Aqeel, Adel Awaji
INTRODUCTION: Spinal muscular atrophy (SMA) is one of the most common genetic causes of death in infants due to a mutation of the motor neuron 1 (SMN1) gene. The SMN1 gene encodes for the multifunctional SMN protein. SMN has been shown to be implicated in pre-mRNA splicing, mRNA transport and translational control. Also other mRNA processing proteins, such as GLE1, Marten (MART3) and Fused in Sarcoma (FUS), have been linked to neurodegenerative diseases. The aim of the study was to determine the expression of SMN, GLE1, MART3 and FUS genes in cell lines of the fibroblasts derived from SMA patients and normal controls...
December 19, 2018: Folia Histochemica et Cytobiologica
Aneta Skoniecka, Agata Zauszkiewicz-Pawlak, Agata Tyminska, Miroslawa Cichorek
INTRODUCTION: The effect of melanogenesis intensity on melanoma biology remains an open question, and the biological differences between melanotic and amelanotic melanoma cells have not yet been satisfactorily documented. As a result, the melanization of melanoma cells in in vitro cultures is not considered among experimental procedures. The aim of this study was to investigate the effect of the medium used to culture Bomirski amelanotic Ab melanoma cells on the melanogenesis process...
October 29, 2018: Folia Histochemica et Cytobiologica
Lukasz P Bialy, Jean Fayet, Grzegorz M Wilczyński, Izabela Mlynarczuk-Bialy
INTRODUCTION: The main component of extralysosomal proteolysis is the ubiquitin-proteasome system (UPS), which is supplemented by tripeptidyl peptidase II (TPPII). That system is a target for anticancer strategies by using proteasome inhibitors. Data from several studies on leukemic cells share evidence for the beneficial and potential role of TPPII in cell survivability. Therefore, the aim of this work was to analyze the effect of AAF-cmk, a membrane permeable semi-specific TPPII inhibitor, on human monocytic leukemic cells U937 for translational research...
October 8, 2018: Folia Histochemica et Cytobiologica
Lucyna Kaszubowska, Jerzy Foerster, Przemysław Kwiatkowski, Daria Schetz
INTRODUCTION: NKT-like cells are "non-classical", "CD1d-independent" NKT cells which represent highly differentiated, conventional T lymphocytes coexpressing several NK (natural killer) associated receptors. They are effector lymphocytes of both innate and adaptive immunity and simultaneously regulatory cells of the adaptive immune system. They reveal large granular lymphocyte morphology and can mediate both MHC-restricted and MHC-unrestricted cytotoxicity, secrete many cytokines and modulate Th1 immune responses...
2018: Folia Histochemica et Cytobiologica
Hui Shi, Juan Liu, Peng Zhu, Haiyan Wang, Zhenjun Zhao, Guohong Sun, Jianyuan Li
INTRODUCTION: High levels of reactive oxygen species (ROS) have potential toxic effects on testicular function and sperm quality. Peroxiredoxins (PRDXs) are enzymes with a role as ROS scavenger. The aim of the study was to reveal the presence and localization of PRDXs in human testis, epididymis and spermatozoa, and the protective roles of PRDX2 and PRDX6 in sperm motility. MATERIAL AND METHODS: The presence and localization of PRDXs in the human testis, epididymis and spermatozoa were detected by immunohistochemistry, western blot and immunofluorescence...
2018: Folia Histochemica et Cytobiologica
Grazyna Pyka-Fosciak, Mariusz Stasiolek, Jan A Litwin
INTRODUCTION: Experimental autoimmune encephalomyelitis (EAE) is a widely used animal model for studying immunopathology of multiple sclerosis (MS) because it repeats the hallmarks of the human disease, such as focal inflammation and demyelination of the central nervous system, subsequently leading to axonal and neuronal loss. The interrelationships, timing and sequence of different pathological processes that lead to histologically observed lesions in SM are still incompletely understood...
2018: Folia Histochemica et Cytobiologica
Barbara Grzechocinska, Filip A Dabrowski, Anna Cyganek, Marcin Chlebus, Christopher Kobierzycki, Lukasz Michalowski, Barbara Gornicka, Miroslaw Wielgos
INTRODUCTION: Endometrium undergoes regular, cyclic tissue remodeling mostly associated to the endocrine system status. It is well-known fact that steroid hormones are strongly responsible for changes in endometrium. The precise mechanism of their action is still under investigation. The aim of the study was to evaluate the expression of metalloproteinases 2 and 7 (MMP-2, -7) and tissue inhibitor of metalloproteinase 1 (TIMP-1) in human endometrium in relation to serum concentrations of estradiol and progesterone during different phases of menstrual cycle...
2018: Folia Histochemica et Cytobiologica
Mohammad Yousef, Elara N Moudilou, Hafsa Djoudad-Kadji, Jean-Marie Exbrayat
INTRODUCTION: Typhlonectes compressicauda is a viviparous gymnophionan amphibian living in tropical areas of South America. This lengthened amphibian is submitted to seasonal variations characterized by the rainy season (from January to June) and the dry season (from July to December). The mineral homeostasis in amphibians is partly ensured by the neurohormones arginine-vasotocin (AVT), and mesotocin (MST). These two hormones were localized in the hypothalamus, and their receptors, mesotocin receptors (MTR) and vasotocin receptors (VTR2) in the kidney...
2018: Folia Histochemica et Cytobiologica
Sherif Mohamed Zaki, Enas Ahmed Mohamed, Shereen Abdel Fattah, Hend Abdullah, Lucyna Kaszubowska
INTRODUCTION: Aging causes morphological and functional changes in the thyroid gland. Free radicals play a key role in the pathology of normal aging. Vimentin and cytokeratin are cytoskeletal intermediate filaments that are often used as indirect indices of tissue injury. The aim of the study was to clarify the age-related alterations in the structure and function of the thyroid gland. The relationship between oxidative/antioxidative stress markers and cytoskeletal intermediate filaments (vimentin and cytokeratin) and oxidative/antioxidative stress markers as well as vascular endothelial growth factor (VEGF) during aging were elucidated...
2018: Folia Histochemica et Cytobiologica
Slawomir Lewicki, Aneta Lewicka, Boleslaw Kalicki, Agnieszka Sobolewska-Ruta, Bogdan Debski, Robert Zdanowski, Tomasz Syryło, Malgorzata Kloc, Jacek Z Kubiak
INTRODUCTION: Genistein (plant isoflavone) is a well-known anti-cancer drug with estrogenic-like properties. Genistein also regulates sugar and lipid metabolism; thus, it has anti-diabetic properties. The aim of the study was to evaluate in vitro effects of genistein on glucose transport, fatty acids oxidation, activation of PKB, and expression of genes related to insulin pathway in differentiated myoblast C2C12 mouse cell line. MATERIAL AND METHODS: Differentiated myoblast C2C12 mouse cell line was used to assess the effects of different genistein concentrations on glucose transport and fatty acids oxidation measured by radioactivity technique, activation of PKB, and expression of selected genes related to insulin signaling pathway (IR-a, IR-b, IRS-1, PKB, GLUT-4, PP2A, SH-PTP2) at the mRNA and protein levels...
2018: Folia Histochemica et Cytobiologica
Cornelia Amalinei, Ioana Păvăleanu, Ludmila Lozneanu, Raluca Balan, Simona-Eliza Giuşcă, Irina-Draga Căruntu
Firstly described at the end of nineteenth century, endometriosis remains an enigmatic disease, from etio-pathogenesis to specific markers of diagnosis and its ability to associate with malignancies. Our review has been designed from a historical perspective and steps up to an updated understanding of the disease, facilitated by relatively recent molecular and genetic progresses. Although the histopathological diagnosis is relatively simple, the therapy is difficult or ineffective. Experimental models have been extremely useful as they reproduce the human disease and allow the testing of different potential modulators or treatment options...
2018: Folia Histochemica et Cytobiologica
Michael Thoene, Janusz Godlewski, Liliana Rytel, Ewa Dzika, Ewa Bejer-Olenska, Joanna Wojtkiewicz
INTRODUCTION: Bisphenol A (BPA) is used in the chemical industry for manufacturing plastics which are used as food packaging. Data indicate that BPA is released from such products and is widely present in the environment and the human body. So far, the EFSA and the US FDA have determined "safe" BPA oral exposure levels, and a large amount of data indicates that BPA is harmful even at low-doses. Our previously performed analyses concerning BPA exposure demonstrated the impact of this substance on parasympathetic and peptidergic nerve fibers present within the liver...
2018: Folia Histochemica et Cytobiologica
Barbara M Piskor, Anna Pryczynicz, Emilia Lubowicka, Katarzyna Miniewska, Justyna Zinczuk, Konrad Zareba, Katarzyna Guzinska-Ustymowicz
INTRODUCTION: Fascins are a group of proteins taking part in the maintenance of a proper structure of the cellular cytoskeleton. Fascin-1 is an actin-bundling protein present in neurons, fibroblasts, endothelial, smooth muscle, dendritic and mesenchymal cells whereas lack of its expression is characteristic of epithelial cells. Fascin-1 overexpression can be observed in neoplastic cells. Therefore, the aim of this study was to assess the expression of Fascin-1 protein in patients with colorectal cancer (CRC) and to analyze associations between Fascin-1 ex-pression and clinical-pathological parameters...
2018: Folia Histochemica et Cytobiologica
Olga Stasikowska-Kanicka, Małgorzata Wągrowska-Danilewicz, Marian Danilewicz
INTRODUCTION: Neuropilins (NRPs) are multifunctional glycoproteins that play an important role in angiogenesis and cancer progression. The aim of the study was to examine the immunoexpression of neuropilin 1 (NRP1), the number of NRP1+ infiltrating cells and CD163+ macrophages, and density of microvessels (MVD) in oral squamous cell carcinoma (OSCC). MATERIAL AND METHODS: The study was performed on 45 OSCC patients with metastases (OSCCM+), 51 patients without metastases (OSCCM-) and 17 control cases...
2018: Folia Histochemica et Cytobiologica
Matthew Dunster-Jones, Michelle Steicke, James Mackie, Rachel Guthrie, Tam Nguyen Dinh, Fahima Ahmady, Jonathan Golledge, Yutang Wang
INTRODUCTION: Ethanol is a commonly used fixative. Fixation of the inner layers of the tissue depends on the ability of the fixative to diffuse into the tissue. It is unknown whether the concentration of ethanol affects its penetration into tissues. This study aimed to compare the penetration rates of 50% and 100% ethanol into bovine heart and liver tissues. MATERIALS AND METHODS: The penetration distance and tissue shrinkage or expansion were measured by analysing the digital images of the heart and liver tissues before and after immersion in ethanol at 20°C for 2, 6, 24 or 30 hours...
2018: Folia Histochemica et Cytobiologica
Katarzyna Ratajczak-Wielgomas, Krzysztof Kassolik, Jedrzej Grzegrzolka, Tomasz Halski, Aleksandra Piotrowska, Katarzyna Mieszala, Iwona Wilk, Marzenna Podhorska-Okolow, Piotr Dziegiel, Waldemar Andrzejewski
INTRODUCTION: Massage is a physiotherapeutic treatment, commonly used in both therapy and restoration of normal body functions. The aim of this work was to determine the effects of skin massage on stimulating the expression of angiogenesis-initiating factors, i.e. VEGF-A, FGF-2 (bFGF) and CD34 and on skin regeneration processes. MATERIAL AND METHODS: The study was conducted on 48 Buffalo strain rats, randomly divided into two groups. In the first group (M, the massaged group), massage was applied five times a week for 7 weeks...
2018: Folia Histochemica et Cytobiologica
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