journal
https://read.qxmd.com/read/38070688/the-capsid-protein-of-citrus-leprosis-virus-c-shows-a-nuclear-distribution-and-interacts-with-the-nucleolar-fibrillarin-protein
#41
JOURNAL ARTICLE
Mikhail Oliveira Leastro, Vicente Pallás, Jesús Ángel Sánchez-Navarro
Brevipalpus-transmitted viruses (BTVs) have a significant negative economic impact on the citrus industry in Central and South America. Until now, only a few studies have explored the intracellular distribution and interaction of BTVs-encoded proteins with host factors, particularly for cileviruses, the main BTV responsible for the Citrus Leprosis (CL) disease. This study describes the nuclear localization of citrus leprosis virus C (CiLV-C) capsid protein (p29) and its interaction with the fibrillarin (Fib2) within the nucleolar compartment and cell cytoplasm...
December 7, 2023: Virus Research
https://read.qxmd.com/read/38065303/new-anti-sars-cov-2-aminoadamantane-compounds-as-antiviral-candidates-for-the-treatment-of-covid-19
#42
JOURNAL ARTICLE
Daisymara Priscila de Almeida Marques, Luis Adan Flores Andrade, Erik Vinicius Sousa Reis, Felipe Alves Clarindo, Thaís de Fátima Silva Moraes, Karine Lima Lourenço, Wellington Alves De Barros, Nathália Evelyn Morais Costa, Lídia Maria de Andrade, Ágata Lopes-Ribeiro, Mariella Sousa Coêlho Maciel, Laura Cardoso Corrêa-Dias, Isabela Neves de Almeida, Thalita Souza Arantes, Vivian Costa Vasconcelos Litwinski, Leonardo Camilo de Oliveira, Mateus Sá Magalhães Serafim, Vinicius Gonçalves Maltarollo, Silvia Cristina Guatimosim Fonseca, Mário Morais Silva, Moriya Tsuji, Rafaela Salgado Ferreira, Luiza Valença Barreto, Edel Figueiredo Barbosa-Stancioli, Flávio Guimarães da Fonseca, Ângelo de Fátima, Jordana Grazziela Alves Coelho Dos Reis
Here, the antiviral activity of aminoadamantane derivatives were evaluated against SARS-CoV-2. The compounds exhibited low cytotoxicity to Vero, HEK293 and CALU-3 cells up to a concentration of 1,000 µM. The inhibitory concentration (IC50 ) of aminoadamantane was 39.71 µM in Vero CCL-81 cells and the derivatives showed significantly lower IC50 values, especially for compounds 3F4 (0.32 µM), 3F5 (0.44 µM) and 3E10 (1.28 µM). Additionally, derivatives 3F5 and 3E10 statistically reduced the fluorescence intensity of SARS-CoV-2 protein S from Vero cells at 10 µM...
December 6, 2023: Virus Research
https://read.qxmd.com/read/38065302/lyszx4-nca-a-new-endolysin-with-broad-spectrum-antibacterial-activity-for-topical-treatment
#43
JOURNAL ARTICLE
Ping Li, Mangmang Shen, Wenjie Ma, Xin Zhou, Jiayin Shen
The prevalence of multidrug-resistant highly virulent Klebsiella pneumoniae (MDR-hvKP) requires the development of new therapeutic agents. Herein, a novel lytic phage vB_KpnS_ZX4 against MDR-hvKP was discovered in hospital sewage. Phage vB_KpnS_ZX4 had a short latent period (5 min) and a large burst size (230 PFU/cell). It can rapidly reduce the number of bacteria in vitro and improve survival rates of bacteremic mice in vivo from 0 to 80% with a single injection of 108 PFU. LysZX4, an endolysin derived from vB_KpnS_ZX4, exhibits potent antimicrobial activity in vitro in combination with ethylenediaminetetraacetic acid (EDTA)...
December 6, 2023: Virus Research
https://read.qxmd.com/read/38056502/rapid-increase-in-salivary-iga-and-broad-recognition-of-spike-protein-following-sars-cov-2-vaccination
#44
JOURNAL ARTICLE
Kenji Ota, Hironori Sakai, Daisuke Sasaki, Fujiko Mitsumoto-Kaseida, Kei Sakamoto, Kosuke Kosai, Hiroo Hasegawa, Takahiro Takazono, Koichi Izumikawa, Hiroshi Mukae, Mya Myat Ngwe Tun, Kouichi Morita, Katsunori Yanagihara
Saliva is a key component of mucosal immunity, which protects the oral cavity from viral infections. However, salivary immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), in terms of immunoglobulin dynamics and recognition, have not been investigated sufficiently. In this study, saliva samples were collected from individuals that received SARS-CoV-2 vaccine, and immunoglobulin G (IgG), IgM, and IgA against whole spike protein and S1 protein were measured. IgA against whole spike protein increased significantly following vaccination, while IgA against S1 protein did not...
December 4, 2023: Virus Research
https://read.qxmd.com/read/38043726/analysis-of-the-molecular-determinants-for-furin-cleavage-of-the-spike-protein-s1-s2-site-in-defined-strains-of-the-prototype-coronavirus-murine-hepatitis-virus-mhv
#45
JOURNAL ARTICLE
Annette Choi, Ekaterina D Kots, Deanndria T Singleton, Harel Weinstein, Gary R Whittaker
We analyzed the spike protein S1/S2 cleavage of selected strains of a prototype coronavirus, mouse hepatitis virus (MHV) by the cellular protease furin, in order to understand the structural requirements underlying the sequence selectivity of the scissile segment. The probability of cleavage of selected MHV strains was first evaluated from furin cleavage scores predicted by the ProP computer software, and then cleavage was measured experimentally with a fluorogenic peptide cleavage assay consisting of S1/S2 peptide mimics and purified furin...
December 1, 2023: Virus Research
https://read.qxmd.com/read/38043725/seven-new-mycoviruses-identified-from-isolated-ascomycetous-macrofungi
#46
JOURNAL ARTICLE
Yan-Jie Zhao, Tsuyoshi Hosoya, Syunichi Urayama, Daisuke Hagiwara
Mycoviruses have been described in all major fungal taxonomic groups. There has been much focus on commercially cultivated basidiomycetous macrofungi, while attention to viruses from ascomycetous macrofungi is lacking. Therefore, in this study, we conducted viral screening against fungal mycelia that were regenerated from ascomycetous macrofungi using agarose gel electrophoresis (AGE) and fragmented and primer-ligated dsRNA sequencing (FLDS). Among the 57 isolates, four isolates were detected with virus-like bands through screening with AGE, and subsequent FLDS analyses determined the viral sequences...
December 1, 2023: Virus Research
https://read.qxmd.com/read/38043724/two-genes-encoded-by-mulberry-crinkle-leaf-virus-mclv-the-v4-gene-enhances-viral-replication-and-the-v5-gene-is-needed-for-mclv-infection-in-nicotiana-benthamiana
#47
JOURNAL ARTICLE
Tao-Tao Han, Jia-Xuan Tang, Miao Fang, Peng Zhang, Pei- Yu Han, Zhen-Ni Yin, Yu Ma, Jian Zhang, Quan-You Lu
Mulberry crinkle leaf virus (MCLV) is a member of the genus Mulcrilevirus, family Geminiviridae. The expression and functions of the V4 and V5 genes encoded by the MCLV genome remain unknown. Here, we confirmed the expression of V4 and V5 by analyzing the V4 and V5 mRNAs and the promoter activity of individual ORFs upstream sequences. The functions of V4 and V5 were investigated by constructing Agrobacterium-mediated infectious clones of wild-type MCLV variant П (MCLV vII), MCLVwt and MCLV vП mutants, such as MCLVmV4 (start codon of V4 ORF mutated), MCLVdV4 (5'-end partial deletion of V4 ORF sequence) and MCLVmV5 (V5 ORF start codon mutated)...
December 1, 2023: Virus Research
https://read.qxmd.com/read/37995964/dusp1-mrna-modulation-during-porcine-circovirus-type-2-and-porcine-reproductive-and-respiratory-syndrome-virus-co-infection-regulates-viruses-replication
#48
JOURNAL ARTICLE
Yaima Burgher-Pulgaron, Chantale Provost, Fernando Alvarez, Europa Meza-Serrano, Marie-Jeanne Pesant, Christopher A Price, Carl A Gagnon
The effects of porcine circovirus type 2b (PCV2b) and porcine reproductive and respiratory syndrome virus (PRRSV) co-infection in epithelial cells of the swine respiratory tract is unknown. In the present study, the newborn pig trachea cell line NPTr-CD163, which is permissive to both viruses, was persistently infected with PCV2b and then with PRRSV. Viral replication, cell viability, cytokines' mRNA expression, and modulation of cellular genes expression were evaluated in infected cells. In NPTr-CD163 co-infection model, PCV2b replication was enhanced while PRRSV replication was suppressed...
December 1, 2023: Virus Research
https://read.qxmd.com/read/38211734/harnessing-crispr-technology-for-viral-therapeutics-and-vaccines-from-preclinical-studies-to-clinical-applications
#49
REVIEW
Farzaneh Zahedipour, Fatemeh Zahedipour, Parvin Zamani, Mahmoud Reza Jaafari, Amirhossein Sahebkar
The CRISPR/Cas system, identified as a type of bacterial adaptive immune system, have attracted significant attention due to its remarkable ability to precisely detect and eliminate foreign genetic material and nucleic acids. Expanding upon these inherent capabilities, recent investigations have unveiled the potential of reprogrammed CRISPR/Cas 9, 12, and 13 systems for treating viral infections associated with human diseases, specifically targeting DNA and RNA viruses, respectively. Of particular interest is the RNA virus responsible for the recent global outbreak of coronavirus disease 2019 (COVID-19), which presents a substantial public health risk, coupled with limited efficacy of current prophylactic and therapeutic techniques...
March 2024: Virus Research
https://read.qxmd.com/read/38389324/distinct-phenotype-of-sars-cov-2-omicron-ba-1-in-human-primary-cells-but-no-increased-host-range-in-cell-lines-of-putative-mammalian-reservoir-species
#50
JOURNAL ARTICLE
Manel Essaidi-Laziosi, Francisco J Pérez-Rodríguez, Catia Alvarez, Pascale Sattonnet-Roche, Giulia Torriani, Meriem Bekliz, Kenneth Adea, Matthias Lenk, Tasnim Suliman, Wolfgang Preiser, Marcel A Müller, Christian Drosten, Laurent Kaiser, Isabella Eckerle
SARS-CoV-2's genetic plasticity has led to several variants of concern (VOCs). Here we studied replicative capacity for seven SARS-CoV-2 isolates (B.1, Alpha, Beta, Gamma, Delta, Zeta, and Omicron BA.1) in primary reconstituted airway epithelia (HAE) and lung-derived cell lines. Furthermore, to investigate the host range of Delta and Omicron compared to ancestral SARS-CoV-2, we assessed replication in 17 cell lines from 11 non-primate mammalian species, including bats, rodents, insectivores and carnivores. Only Omicron's phenotype differed in vitro, with rapid but short replication and efficient production of infectious virus in nasal HAEs, in contrast to other VOCs, but not in lung cell lines...
January 2, 2024: Virus Research
https://read.qxmd.com/read/38347757/recombination-between-non-structural-and-structural-genes-as-a-mechanism-of-selection-in-lagoviruses-the-evolutionary-dead-end-of-an-rhdv2-isolated-from-european-hare
#51
JOURNAL ARTICLE
Patrizia Cavadini, Tiziana Trogu, Roser Velarde, Antonio Lavazza, Lorenzo Capucci
The genus Lagovirus, belonging to the family Caliciviridae, emerged around the 1980s. It includes highly pathogenic species, rabbit hemorrhagic disease virus (RHDV/GI.1) and European brown hare syndrome virus (EBHSV/GII.1), which cause fatal hepatitis, and nonpathogenic viruses with enteric tropism, rabbit calicivirus (RCV/GI.3,4) and hare calicivirus (HaCV/GII.2). Lagoviruses have evolved along two independent genetic lineages: GI (RHDV and RCV) in rabbits and GII (EBHSV and HaCV) in hares. To be emphasized is that genomes of lagoviruses, like other caliciviruses, are highly conserved at RdRp-VP60 junctions, favoring intergenotypic recombination events at this point...
January 2, 2024: Virus Research
https://read.qxmd.com/read/38042373/igm-specific-linear-epitopes-on-the-e2-protein-for-serodiagnosis-of-chikungunya
#52
JOURNAL ARTICLE
Qianlin Li, Jun Dai, Yongxia Shi, Qiang Deng, Conghui Liao, Jicheng Huang, Jiahai Lu
Chikungunya virus (CHIKV) and Dengue virus (DENV) are vector-borne diseases transmitted by Aedes aegypti and Aedes albopictus that pose a significant threat to global public health. Cases of acute Chikungunya fever often present similar clinical symptoms to other vector-borne diseases such as Dengue fever. In regions where multiple vector-borne diseases coexist, CHIKV is often overlooked or misdiagnosed as Dengue virus, West Nile virus, Zika virus or other viral infections, which delays its prevention and control...
November 30, 2023: Virus Research
https://read.qxmd.com/read/38040125/enterovirus-d68-vrna-induces-type-iii-ifn-production-via-mda5
#53
JOURNAL ARTICLE
Chi-Chong Chio, Hio-Wai Chan, Shih-Hsiang Chen, Hsing-I Huang
Enterovirus D68 (EV-D68) primarily spreads through the respiratory tract and causes respiratory symptoms in children and acute flaccid myelitis (AFM). Type III interferons (IFNs) play a critical role in inhibiting viral growth in respiratory epithelial cells. However, the mechanism by which EV-D68 induces type III IFN production is not yet fully understood. In this study, we show that EV-D68 infection stimulates Calu-3 cells to secrete IFN-λ. The transfection of EV-D68 viral RNA (vRNA) stimulated IFN-λ via MDA5...
November 29, 2023: Virus Research
https://read.qxmd.com/read/38036064/tracking-sars-cov-2-variants-during-the-2023-flu-season-and-beyond-in-lebanon
#54
JOURNAL ARTICLE
Ibrahim Al Kodsi, Douaa El Rayes, Jad Koweyes, Charbel Al Khoury, Kelven Rahy, Sergio Thoumi, Marc Chamoun, Hoda Haddad, Jacques Mokhbat, Sima Tokajian
BACKGROUND: Early SARS-CoV-2 variant detection relies on testing and genomic surveillance. The Omicron variant (B.1.1.529) has quickly become the dominant type among the previous circulating variants worldwide. Several subvariants have emerged exhibiting greater infectivity and immune evasion. In this study we aimed at studying the prevalence of the Omicron subvariants during the flu season and beyond in Lebanon through genomic screening and at determining the overall standing and trajectory of the pandemic in the country...
November 28, 2023: Virus Research
https://read.qxmd.com/read/38029800/gambogic-acid-inhibits-hbx-mediated-hepatitis-b-virus-replication-by-targeting-the-dtx1-notch-signaling-pathway
#55
JOURNAL ARTICLE
Xu Wen, Dian Li, Peng Chen, Ming Tan, Hui Zhang, Yuting Liu, Jihua Ren, Shengtao Cheng
BACKGROUND & AIMS: Current antiviral drugs, including nucleoside analogs and interferon, fail to eliminate the HBV covalently closed circular DNA (cccDNA), which serves as a transcript template in infected hepatocytes. Silencing the HBV X protein, which plays a crucial role in cccDNA transcription, is a promising approach to inhibit HBV replication. Therefore, the identification of novel compounds that can inhibit HBx-mediated cccDNA transcription is critical. METHODS: Initially, a compound library consisting of 715 monomers derived from traditional Chinese medicines known for their liver-protecting properties was established...
November 27, 2023: Virus Research
https://read.qxmd.com/read/38029799/asfv-epitope-mapping-by-high-density-peptides-microarrays
#56
JOURNAL ARTICLE
Cloé Desmet, Bruna Coelho-Cruz, Dora Mehn, Pascal Colpo, Ana Ruiz-Moreno
African swine fever (ASF) is an acute, highly contagious and deadly infectious disease. It is a threat to animal health with major potential economic and societal impact. Despite decades of ASF vaccine research, still some gaps in knowledge are hindering the development of a functional vaccine. Worth mentioning are gaps in understanding the mechanism of ASF infection and immunity, as well as the fact that - in case of this disease - virus proteins, so-called protective antigens, responsible for inducing protective immune responses in pigs are not identified yet...
November 27, 2023: Virus Research
https://read.qxmd.com/read/38013142/oxidative-stress-enhances-rotavirus-oncolysis-in-breast-cancer-and-leukemia-except-in-melanoma-with-abundant-matrix
#57
JOURNAL ARTICLE
Marvi Guerrero, Juan Hernández, Luis Gomez, Carlos Guerrero
OBJECTIVES: This study aimed to explore the impact of oxidative stress and extracellular matrix integrity on rotavirus infection in various cancer cells, including breast cancer, acute lymphoblastic leukemia, and melanoma. METHODS: We induced oxidative stress using ROS-inducing drugs (cisplatin, metronidazole, melatonin, valproic acid, doxorubicin, losartan, nitrofurantoin, and DHA) and investigated the effects on viral infection in MCF-7, Reh, A375, B16-F1, and SK-MEL-28 cells and the generation of virions from infected cells by harvesting the supernatants every two hours, reinfecting other cells, and analyzing cell viability and DNA fragmentation...
November 27, 2023: Virus Research
https://read.qxmd.com/read/38016504/a-nano-luciferase-expressing-human-coronavirus-oc43-for-countermeasure-development
#58
JOURNAL ARTICLE
Meghan V Diefenbacher, Thomas J Baric, David R Martinez, Ralph S Baric, Nicholas J Catanzaro, Timothy P Sheahan
The genetic diversity of the coronavirus (CoV) family poses a significant challenge for drug discovery and development. Traditional antiviral drugs often target specific viral proteins from specific viruses which limits their use, especially against novel emerging viruses. Antivirals with broad-spectrum activity overcome this limitation by targeting highly conserved regions or catalytic domains within viral proteins that are essential for replication. For rapid identification of small molecules with broad antiviral activity, assays with viruses representing family-wide genetic diversity are needed...
November 26, 2023: Virus Research
https://read.qxmd.com/read/38008221/the-hc-pro-cistron-of-triticum-mosaic-virus-is-dispensable-for-systemic-infection-in-wheat-but-is-required-for-symptom-phenotype-and-efficient-genome-amplification
#59
JOURNAL ARTICLE
Satyanarayana Tatineni, Jeffrey Alexander, Frank Kovacs
Triticum mosaic virus (TriMV), the type species of the genus Poacevirus in the family Potyviridae, is an economically important wheat curl mite-transmitted wheat-infecting virus in the Great Plains region of the USA. In this study, the functional genomics of helper component-proteinase (HC-Pro) encoded by TriMV was examined using a reverse genetics approach. TriMV with complete deletion of HC-Pro cistron elicited systemic infection in wheat, indicating that HC-Pro cistron is dispensable for TriMV systemic infection...
November 24, 2023: Virus Research
https://read.qxmd.com/read/38008220/drug-repurposing-screens-identify-tubercidin-as-a-potent-antiviral-agent-against-porcine-nidovirus-infections
#60
JOURNAL ARTICLE
Tianliang Wang, Guanmin Zheng, Zilu Chen, Yue Wang, Chenxu Zhao, Yaqin Li, Yixin Yuan, Hong Duan, Hongsen Zhu, Xia Yang, Wentao Li, Wenjuan Du, Yongtao Li, Dongliang Li
The emergence of new coronaviruses poses a significant threat to animal husbandry and human health. Porcine epidemic diarrhea virus (PEDV) is considered a re-emerging porcine enteric coronavirus, which causes fatal watery diarrhea in piglets. Currently, there are no effective drugs to combat PEDV. Drug repurposing screens have emerged as an attractive strategy to accelerate antiviral drug discovery and development. Here, we screened 206 natural products for antiviral activity using live PEDV infection in Vero cells and identified ten candidate antiviral agents...
November 24, 2023: Virus Research
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