Carbohydrate Polymers

Because the traditional preparation methods of cellulose nanocrystals (CNCs) involve chemical pollution issues, in this study, two typical green solvents, alkali/urea solvent (AUS) and deep-eutectic solvent (DES), were used to dissolve insoluble soybean fibers (ISF) extracted from okara and prepare regenerated CNCs (AUS/CNC and DES/CNC), which were further modified by TEMPO oxidation (AUS/T-CNC and DES/T-CNC). The recoveries of AUS and DES were 82.58 % and 84.00 %, respectively. Chemical composition analysis showed high cellulose purity (>95 %) of the regenerated CNCs...

A hybrid ultra-light and porous cellulose aerogel was prepared by extracting cellulose fibers from white paper, alkali/urea as a crosslinker agent, and functionalized with CNTs and pure anatase TiO2 nanoparticles. Since CNTs work as mechanical reinforcement for aerogels, physical and mechanical properties were measured. Besides, since TiO2 acts as a photocatalyst for degrading dyes (rhodamine B and methylene blue), UV-Vis spectroscopy under UV light, visible light, and darkroom was used to evaluate the degradation process...

Polysaccharides are ubiquitous, generally benign in nature, and compatible with many tissues in biomedical situations, making them appealing candidates for new materials such as therapeutic agents and sensors. Fluorescent labeling can create the ability to sensitively monitor distribution and transport of polysaccharide-based materials, which can for example further illuminate drug-delivery mechanisms and therefore improve design of delivery systems. Herein, we review fluorophore selection and ways of appending polysaccharides, utility of the product fluorescent polysaccharides as new smart materials, and their stimulus-responsive nature, with focus on their biomedical applications as environment-sensitive biosensors, imaging, and as molecular rulers...

Bacterial infections would cause pathological inflammation and even generate chronic wound. Herein, a ciprofloxacin (Cip)-loaded mild acid-responsive electrospinning nanofiber membrane (NFM) containing 3-diethylaminopropyl isothiocyanate material grafted glycol chitosan (GC-DEAP) was fabricated to prevent bacterial infection against hemostatic and inflammatory phases of wounds. The presence of Cip and GC-DEAP in the objective NFM (PCL/GC-DEAP/Cip) was confirmed through XRD and FTIR. Meanwhile, PCL/GC-DEAP/Cip NFM exhibited high mechanical profiles, suitable water absorption and water vapour transmission ratio...

The intricate, hostile, and diverse nature of osteomyelitis (OM) poses a challenge for complete bacterial eradication and osteogenesis promotion via conventional treatment. Recently, functional hydrogels exhibiting antibacterial and osteogenic properties emerge as a promising avenue for OM wound healing in clinical practice. However, the preparation procedures and associated costs on cytokine and cell therapies for certain functional hydrogels can be complex and prohibitively expensive. In our research, a hybrid hydrogel dressing has been formulated utilizing carboxymethyl chitosan (CMCS) as the base material, and designed with inherent antibacterial, adhesion, proliferation, and differentiation characteristics, showing promise as a candidate for eradicating infection and promoting bone regeneration...

In recent years, hydrogels derived from natural polymers have gained considerable attention. However, lack of mechanical strength and poor stability has become major lacuna of such systems. Scientists have attempted to resolve this problem by introducing chemical cross-linkers or synthetic modifications of natural polymers. In contrast, biological cross-linkers may be more beneficial due to their cytocompatibility and non-immunogenicity. As a biogelator, amino acids (AA) may be lucrative, yet they remain untapped till date...

Cellulose nanocrystals (CNCs) have shown immense promise in medical applications, especially in cancer treatment, owing to their excellent biocompatibility and potential for functional modifications. Considering the crucial role of the protein reduced glyceraldehyde-phosphate dehydrogenase (GAPDH) in cancer progression, we embarked to immobilize CNCs with GAPDH and fluorescent molecules FITC, creating FCNC-G through regioselective modifications. Furthermore, an accelerated proliferation of cancer cells was observed in the presence of FCNC-G...

The cyclodextrin (CD)-based emulsions exhibit complex instability behaviors such as rapid flocculation and creaming, and how to capture droplet dispersion states of the emulsions remains a great challenge. Here we prepare the CD-based emulsions with different oil-water volume ratios and CD concentrations by using high-pressure homogenization, and characterize the emulsion droplets by using optical microscopy and confocal laser scanning microscopy. We evaluate the effects of homogenization pressure on the stability of the emulsions, identify armored droplets with different surface features, measure interfacial concentrations of adsorbed ICs microcrystals, and observe ejection of the oil/CD inclusion complexes (ICs) microcrystals from the droplet surface...

Nanocellulose finds various applications in advanced electrical devices due to its impressive mechanical properties, thermal stability, and degradability. Cellulose nanocrystals (CNCs) with excellent dielectric properties may act as a fresh dielectric plastic. In this study, a new strategy of small molecule modification was used to improve the dielectric constant, breakdown strength, and band gap of the CNCs. The dipole moments, dipole density, and the anisotropic impact of surface groups on the dielectric constant were studied...

In this study, a new green composite sponge made of chitosan and modified with ammonium ascorbate (ACS-CIT) was synthesized in just 10 min. Compared with CS-CIT (sponge prepared from acetic acid), ACS-CIT exhibits significantly enhanced adsorption performance for CR, with the saturated adsorption capacities increased from 353.667 to 1261.639 mg·g-1 . The adsorption mechanism can be summarized as the generation of more hydrogen bonds, electrostatic attraction, and intra particle diffusion, revealing the addition of ascorbic acid introduced more hydroxyl groups, thereby enhancing the hydrogen bonding force, and the ammonium modification of chitosan improved the electrostatic attraction of the material, resulting in a significant increase in its adsorption capacity...

Breast cancer is one of the most common malignant tumors in women all over the world. Mastectomy is the most effective treatment, but there are serious problems such as high tumor recurrence rate and side effects of chemotherapy. Therefore, there is an urgent need for a therapeutic strategy that can effectively promote postoperative wound healing and inhibit local tumor recurrence. In this study, a 3D printing scaffold based on carbon dots-curcumin nano-drug release (CCNPs) was developed as a local drug delivery platform (named CCNACA using CCNPs, Sodium alginate, Nanoclay and Caffeic Acid grafted Chitosan as raw materials), which has the ability to visualize drug release...

Nonsteroidal anti-inflammatory drugs (NSAIDs) are one of the most frequently used drugs and have been frequently detected in aquatic environments. This paper demonstrates a thermally-activated gelatin-chitosan and amine-functionalized metal-organic framework (UiO-66-NH2 ) aerogel (CGC-MOF), which was successfully synthesized for the efficient removal of ibuprofen (IBP) and naproxen (NPX). Various characterization tools were used to systematically analyze the microstructure and physicochemical properties of the synthesized aerogel...

In this study, our primary objective was to enhance the properties of porous membranes by addressing the limitations associated with phase separation. We employed a non-solvent induced phase separation (NIPS) method, utilizing cellulose acetate (CA) in conjunction with citric acid to fabricate these membranes. Citric acid played a dual role: ensuring a uniform pore structure and cross-linking the CA polymer, thereby enhancing its mechanical strength. This approach resulted in the development of a more robust membrane with superior structural integrity...

Land-based uranium resources are becoming scarce because of the widespread development and use of nuclear energy. Therefore, to make up for the shortage of uranium resources, a new chitosan/carboxymethyl-β-cyclodextrin/quaternary ammonium salt-functionalized amidoxime carbon adsorbent (CSAOCF) was designed and synthesized for extracting uranium from seawater. Experimental studies show that the adsorption of uranium by CSAOCF is a spontaneous endothermic reaction and chemical adsorption. The theoretical maximum adsorption capacity of uranium can reach 726 mg/g at 308 K and pH = 6...

Various starch synthesis and tailoring processes involve prevailing adjustments in the degree of polymerization (DP) of linear α-D-1,4-glucan chains (LGCs) for the improved functional performances. Previous studies indicated that LGCs might hinder the twisted relaxation of 9-(2-carboxy-2-cyanovinyl)-julolidine (CCVJ, a hydrophilic molecular rotor), highlighting CCVJ as a potential in-situ structural probe for LGC. In this study, glucose and its α-D-1,4 oligomers and polymers with molecular weights ranging from 0...

The use of immune checkpoint blockade (ICB) is a promising approach for clinical cancer treatment. However, most of cancer patients do not respond to anti-PD-1/PD-L1 antibody. In this study, we proposed a novel strategy of antibody-β-glucan conjugates (AGC) to enhance the antitumor immune response to ICB therapy. The AGC were constructed by conjugating an anti-PD-L1 antibody with a β-glucan via click chemistry. This design facilitates the delivery of β-glucan into the tumor microenvironment (TME)...

Carrageenans are linear sulfated galactans synthesized in the Gigartinales, Rhodophyceae species with a varied range of biological properties that are of value to the pharmaceutical and cosmetic sectors. It is unknown how the fine structure of carrageenans dictates their capacity to affect molecular and cellular responses important to wound healing, or the ability to mitigate oxidative, hemostatic and inflammatory processes. Here we use specific endo-carrageenases, from the marine bacterium Zobellia galactanivorans, to produce enzymatically defined neo-series oligosaccharides from carrageenans with 3,6-anhydro-D-galactose on the non-reducing end...

Gene therapy, as a revolutionary treatment, has been gaining more and more attention. The key to gene therapy is the selection of suitable vectors for protection of exogenous nucleic acid molecules and enabling their specific release in target cells. While viral vectors have been widely used in researches, non-viral vectors are receiving more attention due to its advantages. Chitosan (CS) has been widely used as non-viral organic gene carrier because of its good biocompatibility and its ability to load large amounts of nucleic acids...

Chitosan-based thermosensitive bioink can be a potential option as bioinks for bone tissue engineering because of their excellent biocompatibility and crosslinker-free gelation at physiological temperature. However, their low mechanical strength, poor printability, and low post-printing cell viability are some of their limitations. In this work, self-assembled nanofibrous aggregates of chitosan and gelatin were prepared and incorporated in chitosan-based bioinks to enhance printability, mechanical properties, post-printing cell viability, and proliferation...

Motivated by the quest for biocompatibility, we report on oil-in-water (O/W), high-internal-phase Pickering emulsions stabilized via complexes of mechanical cellulose nanofibrils (CNF) and food-grade cationic surfactant ethyl lauroyl arginate (LAE). The complexation of oppositely charged CNF and LAE can be held together by electrostatic interaction. Their effect on suspensions electrostatic stabilization, heteroaggregation state, and emulsifying ability was studied and related to properties of resultant interfacial tension between oil and water and 3D printing of emulsions...