Systematic and Applied Microbiology | Page 2

Bethany J Pettifor, James Doonan, Sandra Denman, James E McDonald
Acute oak decline (AOD) affects native UK oak species causing rapid decline and mortality in as little as five years. A major symptom of AOD is black weeping stem lesions associated with bacterial phytopathogens, Brenneria goodwinii and Gibbsiella quercinecans. However, there is limited knowledge on the ecological and environmental reservoirs of these phytopathogens. Rainwater and soils are common reservoirs of plant pathogens in a forest environment; therefore, the aim of this study was to investigate the survival of B...
January 3, 2020: Systematic and Applied Microbiology
Ramon Rosselló-Móra, Karl Heinz Schleifer
No abstract text is available yet for this article.
January 2020: Systematic and Applied Microbiology
Patrycja Golinska, Maria Del Carmen Montero-Calasanz, Magdalena Świecimska, Adnan Yaramis, Jose M Igual, Alan T Bull, Michael Goodfellow
A polyphasic study was undertaken to establish the taxonomic status of three Modestobacter strains isolated from a high altitude Atacama Desert soil. The isolates, strains 1G6T , 1G14 and 1G50, showed chemotaxonomic and morphological properties characteristic of members of the genus Modestobacter. The peptidoglycan contained meso-diaminopimelic acid, the whole cell sugars were glucose and ribose (diagnostic sugars) and arabinose, the predominant menaquinone was MK-9(H4 ), polar lipid patterns contained diphosphatidylglycerol, glycophosphatidylinositol, phosphatidylethanolamine (diagnostic component), phosphatidylglycerol and phosphatidylinositol while whole cellular fatty acid profiles consisted of complex mixtures of saturated, unsaturated iso- and anteiso-components...
December 23, 2019: Systematic and Applied Microbiology
Svetlana N Dedysh, Irina S Kulichevskaya, Alexey V Beletsky, Anastasia A Ivanova, W Irene C Rijpstra, Jaap S Sinninghe Damsté, Andrey V Mardanov, Nikolai V Ravin
Pirellula-like planctomycetes are ubiquitous aquatic bacteria, which are often detected in anoxic or micro-oxic habitats. By contrast, the taxonomically described representatives of these bacteria, with very few exceptions, are strict aerobes. Here, we report the isolation and characterization of the facultatively anaerobic planctomycete, strain PX69T , which was isolated from a boreal lake. Its 16S rRNA gene sequence is affiliated with the Pirellula-related Pir4 clade, which is dominated by environmental sequences retrieved from a variety of low-oxygen habitats...
December 19, 2019: Systematic and Applied Microbiology
Takwa Gritli, Walid Ellouze, Saif-Allah Chihaoui, Fathi Barhoumi, Ridha Mhamdi, Bacem Mnasri
Nodulation and genetic diversity of native rhizobia nodulating Lathyrus cicera plants grown in 24 cultivated and marginal soils collected from northern and central Tunisia were studied. L. cicera plants were nodulated and showed the presence of native rhizobia in 21 soils. A total of 196 bacterial strains were selected and three different ribotypes were revealed after PCR-RFLP analysis. The sequence analysis of the rrs and two housekeeping genes (recA and thrC) from 36 representative isolates identified Rhizobium laguerreae as the dominant (53%) rhizobia nodulating L...
December 16, 2019: Systematic and Applied Microbiology
Mami Tanaka, Bi Hongyu, Chunqi Jiang, Sayaka Mino, Pedro Milet Meirelles, Fabiano Thompson, Bruno Gomez-Gil, Tomoo Sawabe
Two novel strains C4III282T and C4III291 were isolated from seawater collected a site off the Taketomi coral reef. Phylogenetic analysis based on the 16S rRNA sequences revealed that the two strains belong to the genus Vibrio. MLSA using eight protein-coding genes (ftsZ, gapA, gyrB, mreB, pyrH, recA, rpoA, and topA) showed that C4III282T and C4III291 are closely related to the members of the Ponticus clade, namely Vibrio panuliri JCM 19500T , Vibrio ponticus DSM 16217T , and "Vibrio rhodolitus" G98...
December 10, 2019: Systematic and Applied Microbiology
Joachim Spergser, Annelise Botes, Tyron Nel, Werner Ruppitsch, Sarah Lepuschitz, Stefan Langer, Sally Ries, Nora Dinhopl, Michael Szostak, Igor Loncaric, Hans-Jürgen Busse
Twelve Mycoplasma (M.) strains isolated from the nose, the trachea, and the lung of ostriches (Struthio camelus) displaying respiratory disease were investigated. Analysis of 16S rRNA gene sequences placed five of these strains within the M. synoviae cluster, and seven strains within the M. hominis cluster of genus Mycoplasma, which was further confirmed by analyses of the 16S-23S rRNA intergenic spacer region, and partial rpoB gene and amino acid sequences. Genomic information as well as phenotypic features obtained by matrix-assisted laser desorption ionization time of flight (MALDI-ToF) mass spectrometry analysis and serological reactions indicated that the strains examined are representatives of two hitherto unclassified species of genus Mycoplasma, for which the names Mycoplasma nasistruthionis sp...
December 9, 2019: Systematic and Applied Microbiology
José David Flores-Félix, Esther Menéndez, Alvaro Peix, Paula García-Fraile, Encarna Velázquez
The genus Agrobacterium was created a century ago by Conn who included it in the family Rhizobiaceae together with the genus Rhizobium. Initially, the genus Agrobacterium contained the non-pathogenic species Agrobacterium radiobacter and the plant pathogenic species Agrobacterium tumefaciens and Agrobacterium rhizogenes. At the end of the past century two new pathogenic species, Agrobacterium rubi and Agrobacterium vitis, were added to the genus. Already in the present century these species plus Agrobacterium larrymoorei were reclassified into genus Rhizobium...
November 29, 2019: Systematic and Applied Microbiology
M Royo-Llonch, P Sánchez, J M González, C Pedrós-Alió, S G Acinas
Cultivable bacteria represent only a fraction of the diversity in microbial communities. However, the official procedures for classification and characterization of a novel prokaryotic species still rely on isolates. Nevertheless, due to single cell genomics, it is possible to retrieve genomes from environmental samples by sequencing them individually, and to assign specific genes to a specific taxon, regardless of their ability to grow in culture. In this study, a complete description was performed for uncultured Kordia sp...
November 22, 2019: Systematic and Applied Microbiology
María Julia Estrella, María Florencia Fontana, Liz Marjory Cumpa Velásquez, Gonzalo Arturo Torres Tejerizo, Luis Diambra, Lars Hestbjerg Hansen, Mariano Pistorio, Analía Inés Sannazzaro
Three symbiotic nitrogen-fixing bacteria (BD68T , BD66 and BD73) isolated from root nodules of Lotus tenuis in lowland soils of the Flooding Pampa (Argentina), previously classified as members of the Mesorhizobium genus, were characterized in this study. Phylogenetic analysis of their 16S rRNA gene sequences showed a close relationship to M. japonicum MAFF 303099T , M. erdmanii USDA 3471T , M. carmichaelinearum ICMP 18942T , M. opportunistum WSM 2975T and M. jarvisii ATCC 33699T , with sequence identities of 99...
November 22, 2019: Systematic and Applied Microbiology
Pilar Martínez-Hidalgo, José David Flores-Félix, Encarna Velázquez, Lambert Brau, Martha E Trujillo, Eustoquio Martínez-Molina
The genus Micromonospora has been found in nodules of several legumes and some new species of this genus were isolated from these plant organs. In this study we analysed the taxonomic diversity of Micromonospora strains isolated from alfalfa nodules in Spain and Australia on the basis of three phylogenetic markers, the rrs and gyrB genes and 16S-23S intergenic spacer (ITS). The genome analysis of selected strains representative of different clusters or lineages found after rrs, gyrB and ITS analyses confirmed the results obtained with these phylogenetic markers...
November 22, 2019: Systematic and Applied Microbiology
Ana L Diéguez, Sabela Balboa, Jesús L Romalde
Two Gram-negative strains obtained from tank water in a scallop hatchery in Norway, were phenotypically and genotypically characterized in order to clarify their taxonomic position. On the basis of 16S rRNA gene sequence analysis, these isolates, ATF 5.2T and ATF 5.4T , were included in the genus Halomonas, being their closest relatives H. smyrnensis and H. taeanensis, with similarities of 98.9% and 97.7%, respectively. Sequence analysis of the housekeeping genes atpA, ftsZ, gyrA, gyrB, mreB, rpoB, rpoD, rpoE, rpoH, rpoN and rpoS clearly differentiated the isolates from the currently described Halomonas species, and the phylogenetic analysis using concatenated sequences of these genes located them in two robust and independent branches...
November 17, 2019: Systematic and Applied Microbiology
Martha Helena Ramírez-Bahena, José David Flores-Félix, Encarna Velázquez, Álvaro Peix
Leucaena leucocephala is a Mimosoid legume tree indigenous to America that has spread to other continents, although it is not still present in some European countries such as Portugal. Nevertheless, we found that this legume can be nodulated in this country by slow-growing rhizobial strains which were identified as Bradyrhizobium canariense trough the analysis of the core genes recA and glnII. The analysis of the symbiotic gene nodC showed that these strains belong to the symbiovar genistearum, which commonly nodulates Genistoid legumes...
November 16, 2019: Systematic and Applied Microbiology
Daniel Jaén-Luchoro, Lucia Gonzales-Siles, Roger Karlsson, Liselott Svensson-Stadler, Kent Molin, Sofia Cardew, Susanne Jensie-Markopolous, Maria Ohlén, Elisabeth Inganäs, Susann Skovbjerg, Brian J Tindall, Edward R B Moore
Clinical and environmental-associated strains (n=17), genotypically related to Corynebacterium spp., yet distinct from any species of the genus Corynebacterium with validly published names, have been isolated during the last 20 years and tentatively identified as Corynebacterium sanguinis, although the combination, "Corynebacterium sanguinis" was never validly published. The comprehensive genotypic and phenotypic characterisations and genomic analyses in this study support the proposal for recognizing the species within the genus Corynebacterium, for which the name, Corynebacterium sanguinis sp...
November 14, 2019: Systematic and Applied Microbiology
Ana S Ramírez, Orestes M Vega-Orellana, Tomeu Viver, José B Poveda, Rubén S Rosales, Carlos G Poveda, Joachim Spergser, Michael P Szostak, M José Caballero, Lorenzo Ressel, Janet M Bradbury, M Mar Tavío, Smruthi Karthikeyan, Rudolf Amann, Konstantinos T Konstantinidis, Ramon Rosselló-Móra
No abstract text is available yet for this article.
November 4, 2019: Systematic and Applied Microbiology
Agnieszka Kuźniar, Kinga Włodarczyk, Jarosław Grządziel, Weronika Goraj, Anna Gałązka, Agnieszka Wolińska
The main goal of the study was to determine the structure of endophytic bacteria inhabiting different parts (endosperm, germ, roots, coleoptiles, and leaves) of two wheat species, Triticum aestivum L. (cv. 'Hondia') and Triticum spelta L. (cv. 'Rokosz'), in order to provide new knowledge about the stability and/or changeability of the core microbiome in different plant organs. The endophytic core microbiome is associated with plants throughout their whole life cycle; however, plant organs can determine the actual endophytic community...
October 21, 2019: Systematic and Applied Microbiology
Christian Abendroth, Adriel Latorre-Pérez, Manuel Porcar, Claudia Simeonov, Olaf Luschnig, Cristina Vilanova, Javier Pascual
Conventional anaerobic digesters intended for the production of biogas usually operate in complete darkness. Therefore, little is known about the effect of light on their microbial communities. In the present work, 16S rRNA gene amplicon Nanopore sequencing and shotgun metagenomic sequencing were used to study the taxonomic and functional structure of the microbial community forming a biofilm on the inner wall of a laboratory-scale transparent anaerobic biodigester illuminated with natural sunlight. The biofilm was composed of microorganisms involved in the four metabolic processes needed for biogas production, and it was surprisingly rich in Rhodopseudomonas faecalis, a versatile bacterium able to carry out photoautotrophic metabolism when grown under anaerobic conditions...
October 19, 2019: Systematic and Applied Microbiology
Michał Kalita, Wanda Małek
A collection of 18 previously unstudied strains isolated from root nodules of Genista germanica (German greenweed) grown in southeast Poland was evaluated for the level of genetic diversity using the BOX-PCR technique and the phylogenetic relationship based on both core (16S rRNA, dnaK, ftsA, glnII, gyrB, recA, rpoB) and nodulation (nodC and nodZ) gene sequences. Each of the 18 G. germanica root nodule isolates displayed unique BOX-PCR patterns, indicating their high level of genomic heterogeneity. Based on the comparative 16S rDNA sequence analysis, 12 isolates were affiliated to the Bradyrhizobium genus and the other strains were most similar to Rhizobium species...
October 19, 2019: Systematic and Applied Microbiology
Julian A Schuster, Andreas Klingl, Rudi F Vogel, Matthias A Ehrmann
Microbiota analysis of blown pack spoiled salami revealed five distinguishable Lactobacillus isolates we could not assign to a known species. Two of the isolates (TMW 1.2172T and TMW 1.1920) are rod-shaped, whilst three isolates (TMW 1.2098T , TMW 1.2118 and TMW 1.2188) appear coccus shaped or as short rods. All isolates are Gram-stain positive, facultative anaerobic, catalase and oxidase negative, non-motile and non-sporulating. Phylogenetic analysis of the 16S rRNA, dnaK, pheS and rpoA gene sequences revealed two distinct lineages within the genus Lactobacillus (L...
October 12, 2019: Systematic and Applied Microbiology
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