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Journals Diagnostic Microbiology and In...

Diagnostic Microbiology and Infectious Disease

https://read.qxmd.com/read/38604075/antigen-functionalized-turnip-mosaic-virus-nanoparticles-increase-antibody-sensing-in-saliva-a-case-study-with-sars-cov-2-rbd
#1
JOURNAL ARTICLE
Carlos Medrano-Arranz, Sara Rincón, Lucía Zurita, Fernando Ponz, Daniel A Truchado
Nanoparticles derived from plant viruses play an important role in nanomedicine due to their biocompatibility, self-assembly and easily-modifiable surface. In this study, we developed a novel platform for increasing antibody sensing using viral nanoparticles derived from turnip mosaic virus (TuMV) functionalized with SARS-CoV-2 receptor binding domain (RBD) through three different methods: chemical conjugation, gene fusion and the SpyTag/SpyCatcher technology. Even though gene fusion turned out to be unsuccessful, the other two constructs were proven to significantly increase antibody sensing when tested with saliva of patients with different infection and vaccination status to SARS-CoV-2...
April 7, 2024: Diagnostic Microbiology and Infectious Disease
https://read.qxmd.com/read/38604077/increased-rheumatoid-factor-production-in-patients-with-severe-covid-19
#2
JOURNAL ARTICLE
Mourad Elghali, I Bannour, I Touil, M Changuel, Y Brahem, O Jaoued, N Boudawara, H Ibn Hadj Amor, S Elatrous, J Knani, N Sakly
BACKGROUND: Searching for Rheumatoid Factors (RF) in patients with coronavirus disease (COVID-19) has rarely been described. OBJECTIVES: To investigate the association between RF isotypes (IgM, IgA, and IgG) and different clinical presentations of COVID-19 in a series of Tunisian patients. STUDY DESIGN: Eighty-two COVID-19 patients were enrolled in this study. Symptomatic cases were recruited from the Department of COVID-19 and the intensive care unit (ICU) of the University Hospital of Mahdia, Tunisia, from January 2021 to March 2021...
April 4, 2024: Diagnostic Microbiology and Infectious Disease
https://read.qxmd.com/read/38604076/evaluation-of-the-sars-cov-2-rna-detection-reagent-lampdirect-genelyzer-kit-using-nasopharyngeal-swab-and-saliva-samples
#3
JOURNAL ARTICLE
Junko S Takeuchi, Kento Fukano, Yumiko Kito, Kei Yamamoto, Moto Kimura
The LAMPdirect Genelyzer KIT allows for the detection of SARS-CoV-2 RNA in saliva samples with a loop-mediated isothermal amplification (LAMP) method and generates results within 20 min. It has been approved by the Pharmaceuticals and Medical Devices Agency in Japan. In this study, the performance of the LAMPdirect Genelyzer KIT was compared with that of the RT-qPCR reference method using 50 nasopharyngeal swabs and 100 saliva samples. In addition, we evaluated the applicability of an alternative reverse transcriptase and the effects of an inactivation buffer...
April 4, 2024: Diagnostic Microbiology and Infectious Disease
https://read.qxmd.com/read/38598951/post-pandemic-trends-epidemiological-and-etiological-insights-into-acute-respiratory-infections-in-southern-china
#4
JOURNAL ARTICLE
Yuquan Gao, Xudong Feng, Ting Yuan, Min Li, Muyun Wei, Shuangshuang Li
Data on people suspected with acute respiratory infections (ARIs) from August 2022 to December 2023 in southern China were analyzed. Following the COVID-19 pandemic, the positive detection rates of respiratory pathogens increased to 56.9%. Influenza A virus (IAV) emerged as the predominant prevalence pathogen (52.1%), followed by Mycoplasma pneumoniae (Mp: 21.2%), and SARS-CoV-2 (11.6%). Mp, IAV, and Human rhinovirus (HRV) infection were the primary etiologies of ARIs patients under age 18, accounting for 49...
April 4, 2024: Diagnostic Microbiology and Infectious Disease
https://read.qxmd.com/read/38581928/the-characteristics-of-the-gut-microbiota-in-patients-with-pulmonary-tuberculosis-a-systematic-review
#5
REVIEW
Weiran Li, Yunfei Huang, Shuai Tong, Chaomin Wan, Zhiling Wang
Increasing evidence has indicated dysbiosis of the gut microbiota in patients with pulmonary tuberculosis (PTB). However, the change in the intestinal microbiota varies between different studies. This systematic review was conducted to investigate the characteristics of the gut microbiota in PTB patients. The MBASE, MEDLINE, Web of Science, and Cochrane Library electronic databases were systematically searched, and the quality of the retrieved studies was evaluated using the Newcastle-Ottawa scale. A total of 12 studies were finally included in the systematic review...
March 30, 2024: Diagnostic Microbiology and Infectious Disease
https://read.qxmd.com/read/38579431/staphylococcus-aureus-nasal-colonization-and-susceptibility-profile-to-antimicrobials-in-hemodialysis-patients-using-a-protocol-of-seven-collections
#6
JOURNAL ARTICLE
Mariana Abou Mourad Ferreira, Pamella Plass Andrade Pires, Kênia Valéria Dos Santos
BACKGROUND: Patients colonized with Staphylococcus aureus in their nasal passages have a higher risk of acquiring infection, especially if they are immunocompromised or have comorbidities such as chronic renal failure undergoing hemodialysis (HD). OBJECTIVE: This study aimed to report the prevalence of nasal carriage of S. aureus among HD patients utilizing a seven-week sampling protocol and to assess the susceptibility of these isolates to various antimicrobial agents...
March 30, 2024: Diagnostic Microbiology and Infectious Disease
https://read.qxmd.com/read/38574445/proteus-species-bloodstream-infections-comparative-epidemiology-of-three-species
#7
JOURNAL ARTICLE
Kevin B Laupland, Felicity Edwards, Patrick N A Harris
BACKGROUND: Although Proteus species are occasional causes of serious infections, their epidemiology has not been well defined. The objective was to describe the overall and species-specific occurrence and determinants of Proteus species bloodstream infection (BSI) in a large Australian population. METHODS: All Queensland residents with Proteus species BSI identified within the publicly funded healthcare system between 2000 and 2019 were included. RESULTS: A total of 2,143 incident episodes of Proteus species BSI were identified among 2,079 Queensland residents...
March 28, 2024: Diagnostic Microbiology and Infectious Disease
https://read.qxmd.com/read/38574444/effectiveness-of-a-standardized-quality-control-management-procedure-for-covid-19-rt-pcr-testing-a-large-scale-diagnostic-accuracy-study-in-china
#8
JOURNAL ARTICLE
Mengyu Liu, Cuihong Tian, Yequn Chen, Jinxiu Zhu, Yan Zheng, Jianhua Chen, Zhen Li, Feng Xu, Liang Wu, Xingyu Wang, Longxu Xie, Xuerui Tan, Yingmu Cai
BACKGROUND: The study aimed to construct a standardized quality control management procedure (QCMP) and access its accuracy in the quality control of COVID-19 reverse transcriptase-polymerase chain reaction (RT-PCR). METHODS: Considering the initial RT-PCR results without applying QCMP as the gold standard, a large-scale diagnostic accuracy study including 4,385,925 participants at three COVID-19 RT-PCR testing sites in China, Foshan (as a pilot test), Guangzhou and Shenyang (as validation sites), was conducted from May 21, 2021, to December 15, 2022...
March 28, 2024: Diagnostic Microbiology and Infectious Disease
https://read.qxmd.com/read/38555835/viral-infection-a-missed-cause-of-chronic-spontaneous-urticaria
#9
LETTER
Albert C Chong, David A Khan, Jeffrey D Klausner
No abstract text is available yet for this article.
March 28, 2024: Diagnostic Microbiology and Infectious Disease
https://read.qxmd.com/read/38574446/impact-of-sphingolipid-synthesis-inhibition-on-the-drug-susceptibility-patterns-of-trichophyton-species
#10
JOURNAL ARTICLE
Khushboo Arya, Sana Akhtar Usmani, Nitin Bhardwaj, Manoj Kumar, Shivaprakash M Rudramurthy, Rajendra Prasad, Ashutosh Singh
The well known dermatophyte infections caused by Trichophyton species are an ambiguous problem to treat using the present arsenal of antifungals. This study expounds on the effect of inhibition of sphingolipid pathway on Trichophyton growth. Findings from the drug susceptibility assays suggest sphingolipid inhibition severely restricts the growth of T. interdigitale and T. tonsurans. The observed synergistic effects of combinations of sphingolipid inhibitor and conventional drugs provide a promising treatment strategy against Trichophyton infection...
March 24, 2024: Diagnostic Microbiology and Infectious Disease
https://read.qxmd.com/read/38547800/multi-and-extensively-drug-resistant-escherichia-coli-isolated-from-south-african-children-under-5-years-old-with-diarrhoea
#11
JOURNAL ARTICLE
Lee Heine, Ntando W Alfinete, Natasha Potgieter, Tobias G Barnard
BACKGROUND: Globally, millions of children die as a result of diarrhoea and/or antimicrobial resistant infections. Diarrhoeagenic Escherichia coli (DEC) are responsible for a substantial proportion of cases of diarrhoea in South Africa and sub-Saharan Africa. Effective treatments (including the use of antimicrobials) are therefore essential. METHODOLOGY: E. coli isolated from children under the age of five were subjected to antimicrobial susceptibility testing using the Vitek 2® compact automated system (bioMérieux Inc...
March 24, 2024: Diagnostic Microbiology and Infectious Disease
https://read.qxmd.com/read/38537507/differential-host-responses-to-covid-19-unraveling-the-complexity
#12
JOURNAL ARTICLE
Vinod Kumar Verma, Rahul Yadav, Syed Sultan Beevi, Annie S Mohod, Shravya Mancharla, Neha Damodar, Radhika Chowdary Darapuneni, Sukurtha Gopal Reddy, Pavani Upendram, Mohammad Nawaz Salt, Praveen Kulkarni, Sambit Sahu, Bhaskar Rao Bollineni
These diverse outcomes of Covid-19 are influenced by various factors including age, gender, underlying health conditions, immune responses, viral variants, external factors, and overall quality of life. Demographic analysis of patients aged 0-18 years experienced mild to moderate cases, above 55 years with co-morbidities, were more severely affected.COVID-19 incidence was higher in males (58 %) & (42 %) in females. The reduced expression of Toll-like receptors (TLR) in severe and critical patients is a crucial determinant...
March 24, 2024: Diagnostic Microbiology and Infectious Disease
https://read.qxmd.com/read/38522369/sucrose-fermenting-salmonella-typhimurium-n23-2364-a-challenge-for-the-diagnostic-laboratory
#13
Marc J A Stevens, Magdalena Nüesch-Inderbinen, Jule Anna Horlbog, Andrea Diethelm, Roger Stephan
We describe a case of Salmonella infection caused by a sucrose-fermenting Salmonella enterica Typhimurium sequence type 12 which acquired transposon CTnscr94 carrying the sucrose operon scrKYABR. Sucrose-fermenting Salmonella are particularly challenging for culture-based detection and may lead to failure to detect Salmonella in clinical samples.
March 20, 2024: Diagnostic Microbiology and Infectious Disease
https://read.qxmd.com/read/38613950/extensively-drug-resistant-salmonella-typhi-leading-to-relapsed-urinary-tract-infection-a-case-report
#14
Muhammad Yasir, Muzeer Ahmed, Muhammad Ateeb Sheikh, Ayesha Saleem, Waqas Siddiqui, Hassan Aziz, Abdul Nafey Kazi, Lubna Iqbal Abbasi, Muhammad Junaid Patel
Salmonella enterica serotype Typhi (S Typhi) associated urinary tract infections are exceedingly rare, accounting for less than 1% of cases. Such infections have known to occur in immune-compromised or individuals with urogenital structural abnormalities. With the emergence of extensively drug resistant S Typhi strains in Pakistan, the management of its various unique presentations poses therapeutic challenges. We report the first documented case of a 74 years old male patient presenting with relapsed urinary tract infection secondary to extensively drug resistant S Typhi...
March 19, 2024: Diagnostic Microbiology and Infectious Disease
https://read.qxmd.com/read/38522370/rapid-identification-fluconazole-and-micafungin-susceptibility-testing-of-candida-species-from-blood-culture-by-a-short-incubation-method
#15
JOURNAL ARTICLE
Ândrea Celestino de Souza, Dariane Castro Pereira, Patricia Orlandi Barth, Eliane Würdig Roesch, Larissa Lutz, Valério Rodrigues Aquino, Luciano Zubaran Goldani
This study aimed to develop and validate a rapid method for identification by MALDI-TOF system and determination of the susceptibility to Fluconazole and Micafungin by broth microdilution among Candidaspecies causing bloodstream infections. Subcultures from blood culture bottles were incubated for 5 hours (+/- 1h) and used to perform the tests, so that the turnaround time of rapid identification and susceptibility profile was about 5 and 24 hours, respectively. The rapid identification showed agreement of 92...
March 19, 2024: Diagnostic Microbiology and Infectious Disease
https://read.qxmd.com/read/38507963/letter-to-the-editor-about-the-strategy-for-performing-treponemal-tests-in-reverse-sequence-algorithms-of-syphilis-diagnosis-establishment-of-a-threshold-for-automated-treponemal-screening-tests-using-the-elecsys-syphilis-assay-roche-diagnostics-mannheim-germany
#16
LETTER
Sara Cherkaoui, Sigi Van den Wijngaert, Hafid Dahma, Delphine Martiny
No abstract text is available yet for this article.
March 19, 2024: Diagnostic Microbiology and Infectious Disease
https://read.qxmd.com/read/38537505/application-of-bacttiter-glo-atp-bioluminescence-assay-for-mycobacterium-tuberculosis-detection
#17
JOURNAL ARTICLE
Mariana Abou Mourad Ferreira, Laura Candeias Dos Santos, Luiz Guilherme Schmidt Castellani, Manuela Negrelli Brunetti, Moisés Palaci
BACKGROUND: Tuberculosis (TB), caused by Mycobacterium tuberculosis (MTB), remains a global health threat, necessitating faster and more accessible diagnostic methods. This study investigates critical parameters in the application of a commercial ATP bioluminescence assay for the detection of MTB. METHOD: Our objective was to optimize the ATP bioluminescence protocol using BacTiter-Glo™ for MTB, investigating the impact of varying volumes of MTB suspension and reagent on assay sensitivity, evaluating ATP extraction methods, establishing calibration curves, and elucidating strain-specific responses to antimicrobial agents...
March 18, 2024: Diagnostic Microbiology and Infectious Disease
https://read.qxmd.com/read/38518388/comparison-of-mbt-biotargets-with-mbt-steel-targets-for-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-identifications-of-microorganisms-in-a-clinical-microbiology-laboratory
#18
JOURNAL ARTICLE
Robert K P Schouten, Valentijn A Schweitzer, Sebastian van Marm, Rob J Rentenaar
MALDI-TOF MS identifications of microorganisms in a clinical laboratory were investigated, comparing steel targets with MBT Biotargets. By using MBT Biotargets, the score values of yeast identifications increased, whereas the score values of Gram-negative bacteria decreased. Switching to MBT Biotargets did not negatively impact overall frequencies of high confidence identifications.
March 18, 2024: Diagnostic Microbiology and Infectious Disease
https://read.qxmd.com/read/38507964/safety-of-an-inactivated-covid-19-vaccine-coronavac-in-children-aged-7-14-years-in-taizhou-china
#19
JOURNAL ARTICLE
Dong-Sheng Zhang, Jing-Jing Zhu, Wen-Jie Zheng, Xue-Ping Bao, Liang-Xue Sun
Our study aimed to evaluate the safety of CoronaVac, an inactivated vaccine made by Sinovac, in children aged 7-14. We conducted a parent-administered online survey to monitor adverse reactions after vaccinating children in Taizhou, China, from February 15, 2021, to January 19, 2022. 767 parents completed the survey after receiving a questionnaire via WeChat. Overall, 15.3 % (117/767) of children experienced adverse effects after the first dose, and 12.2 % (88/724) after the second. Muscle pain was the most common adverse reaction post-first dose (10...
March 16, 2024: Diagnostic Microbiology and Infectious Disease
https://read.qxmd.com/read/38513559/mpox-virus-dna-detection-in-different-clinical-specimens-tertiary-level-laboratory-experience-bosnia-and-herzegovina-2022
#20
JOURNAL ARTICLE
Salimović-Bešić Irma, Zahirović Edina, Čamdžić Azra, Dedeić-Ljubović Amela
Mpox virus (MPXV) infection is difficult to distinguish from other (non-)infectious diseases. The etiology of rash can be differentiated by real-time polymerase chain reaction (rtPCR) on different types of samples. The study aims to provide experience with emerging MPXV diagnostics in a tertiary-level laboratory in Bosnia and Herzegovina. From July-December 2022, a total of 18 mpox suspected persons were tested. MPXV infection was confirmed by rtPCR in 10/18 (55.56 %) persons. The number of cases reached a peak in October 2022...
March 15, 2024: Diagnostic Microbiology and Infectious Disease
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