Chaouki Benabdessalem, Wafa Ben Hamouda, Soumaya Marzouki, Rokhaya Faye, Adji Astou Mbow, Babacar Diouf, Oumar Ndiaye, Ndongo Dia, Ousmane Faye, Amadou A Sall, Cheikh Tidiane Diagne, Houda Amellal, Sayeh Ezzikouri, Diary Juliannie Ny Mioramalala, Fanirisoa Randrianarisaona, Khaled Trabelsi, Mohamed Boumaiza, Sonia Ben Hamouda, Rym Ouni, Soumaya Bchiri, Amani Chaaban, Mariem Gdoura, Yousr Gorgi, Imen Sfar, Sadok Yalaoui, Jalila Ben Khelil, Agnes Hamzaoui, Meya Abdallah, Yosra Cherif, Stéphane Petres, Chris Ka Pun Mok, Nicolas Escriou, Sébastien Quesney, Koussay Dellagi, Matthieu Schoenhals, M'hammed Sarih, Inès Vigan-Womas, Jihene Bettaieb, Samia Rourou, Mohamed Ridha Barbouche, Melika Ben Ahmed
Management of the COVID-19 pandemic relies on molecular diagnostic methods supported by serological tools. Herein, we developed S-RBD- and N- based ELISA assays useful for infection rate surveillance as well as the follow-up of acquired protective immunity against SARS-CoV-2. ELISA assays were optimized using COVID-19 Tunisian patients' sera and prepandemic controls. Assays were further validated in 3 African countries with variable endemic settings. The receiver operating curve was used to evaluate the assay performances...
January 22, 2023: Diagnostic Microbiology and Infectious Disease