Journals Diagnostic Microbiology and In...

Diagnostic Microbiology and Infectious Disease
Alberto A Zellner, Gunnar T Hischebeth, Ernst Molitor, Dieter C Wirtz, Thomas M Randau
Oftentimes, Gram-positive cocci are the cause for periprosthetic joint infections (PJI). Most of these infections include bacteria such as Staphylococcus aureus, Staphylococcus epidermidis or other coagulase-negative staphylococci. We here present the first case of a PJI caused by Kytococcus schroeteri. While being a Gram-positive coccus, it is very rarely the cause for infections in the human body. K. schroeteri is part of the micrococcus branch and often encountered as a symbiotic bacterium living on the skin...
February 15, 2023: Diagnostic Microbiology and Infectious Disease
Clément Munier, Céline Dupieux, Camille Kolenda, Anne-Gaelle Ranc, Olivier Dauwalder, Michèle Bes, François Vandenesch, Anne Tristan, Frédéric Laurent
The sensitivity of an immunochromatographic assay for detecting methicillin resistance (PBP2a SA Culture Colony Test, Alere-Abbott) on shortly incubated subcultures of staphylococci in blood cultures was evaluated. The assay is highly sensitive for the detection of methicillin-resistant Staphylococcus aureus after 4 hour-subculture but requires 6 hour-incubation for methicillin-resistant coagulase-negative staphylococci.
February 11, 2023: Diagnostic Microbiology and Infectious Disease
Nayara Carvalho Silva, Maycon Carvalho de Souza, Marco André Loureiro Tonini, Ricardo Pinto Schuenck
We aimed to analyze the molecular epidemiology of 46 methicillin-resistant Staphylococcus aureus (MRSA) isolated from breast infections. The USA300 lineage carrying SCCmecIVa, arginine catabolic mobile element, t008, ST8, and Panton-Valentine leukocidin genes was predominant (93%). This is the first study that describes the spread of the USA300 methicillin-resistant Staphylococcus aureus clone in breast infections in Brazil.
February 10, 2023: Diagnostic Microbiology and Infectious Disease
L Krausser, S M Braet, Z Benaamar, M Van Dyck-Lippens, B C de Jong, L Rigouts
Efficient inactivation of clinical samples containing mycobacteria is crucial for biosafety during shipment and handling. When stored in RNAlater, Mycobacterium tuberculosis H37Ra remains viable, and our results suggest that at -20 °C and 4 °C changes in the mycobacterial transcriptome are possible. Only GTC-TCEP and DNA/RNA Shield inactivate sufficiently for shipment.
February 1, 2023: Diagnostic Microbiology and Infectious Disease
Neena Kanwar, Christopher J Harrison, Morgan A Pence, Xuan Qin, Rangaraj Selvarangan
This study evaluated the in vitro activity of Ceftolozane/tazobactam (C/T) vs 10 comparator agents against Pseudomonas aeruginosa isolates obtained from clinical respiratory samples from pediatric patients with cystic fibrosis at three hospitals during 2015 to 2020. Antimicrobial susceptibility testing was performed using microbroth dilution technique with custom prepared Sensititre® MIC plates. MICs were determined via Sensititre Vizion® system and results were interpreted using current CLSI and EUCAST (2022) breakpoint criteria...
January 26, 2023: Diagnostic Microbiology and Infectious Disease
Chaouki Benabdessalem, Wafa Ben Hamouda, Soumaya Marzouki, Rokhaya Faye, Adji Astou Mbow, Babacar Diouf, Oumar Ndiaye, Ndongo Dia, Ousmane Faye, Amadou A Sall, Cheikh Tidiane Diagne, Houda Amellal, Sayeh Ezzikouri, Diary Juliannie Ny Mioramalala, Fanirisoa Randrianarisaona, Khaled Trabelsi, Mohamed Boumaiza, Sonia Ben Hamouda, Rym Ouni, Soumaya Bchiri, Amani Chaaban, Mariem Gdoura, Yousr Gorgi, Imen Sfar, Sadok Yalaoui, Jalila Ben Khelil, Agnes Hamzaoui, Meya Abdallah, Yosra Cherif, Stéphane Petres, Chris Ka Pun Mok, Nicolas Escriou, Sébastien Quesney, Koussay Dellagi, Matthieu Schoenhals, M'hammed Sarih, Inès Vigan-Womas, Jihene Bettaieb, Samia Rourou, Mohamed Ridha Barbouche, Melika Ben Ahmed
Management of the COVID-19 pandemic relies on molecular diagnostic methods supported by serological tools. Herein, we developed S-RBD- and N- based ELISA assays useful for infection rate surveillance as well as the follow-up of acquired protective immunity against SARS-CoV-2. ELISA assays were optimized using COVID-19 Tunisian patients' sera and prepandemic controls. Assays were further validated in 3 African countries with variable endemic settings. The receiver operating curve was used to evaluate the assay performances...
January 22, 2023: Diagnostic Microbiology and Infectious Disease
Sarah Mafi, Sylvie Rogez, Jérôme Darreye, Sophie Alain, Sébastien Hantz
Lateral flow immunoassays (LFIA) for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies are used for population surveillance and potentially individual risk assessment. The performance of the SureScreen Diagnostics LFIA targeting the spike protein was evaluated in comparison with 3 automated assays (Abbott Alinity-i SARS-CoV-2 IgG, DiaSorin Liaison® SARS-CoV-2 S1/S2 IgG, Wantai SARS-CoV-2 Ab ELISA). We assessed sensitivity using 110 serum samples from PCR confirmed COVID-19 infected patients...
January 10, 2023: Diagnostic Microbiology and Infectious Disease
Annelies De Bel, Annelies Brouwers, Frederick Verbeke, Liselotte Coorevits, Nico Callewaert, Emilie De Muynck, Michaël Boudewijns
OBJECTIVES: To evaluate the analytical performance of the TB-IGRA® assay on the VIDAS3 platform (bioMérieux) when testing a predominantly low risk population in a low incidence area. RESULTS: Eighty-eight percent of the results were concordant between QuantiFERON®-TB Gold-Plus (QFT®-Plus, QIAGEN) and TB-IGRA®. All 12 of 99 (12.1%) discordant results were determined positive only with the TB-IGRA® assay. In 11 of 12 of these discordant cases, no explanation could be found in the medical record...
April 2023: Diagnostic Microbiology and Infectious Disease
Ying Luo, Ying Xue, Wei Liu, Huijuan Song, Yi Huang, Guoxing Tang, Xiaochen Wang, Yimin Cai, Feng Wang, Xueyun Guo, Qi Wang, Ziyong Sun
OBJECTIVES: The study aims to investigate the potential of convolutional neural network (CNN) based on spot image of T-SPOT assay for distinguishing active tuberculosis (ATB) from latent tuberculosis infection (LTBI). METHODS: CNN was applied to recognize and classify T-SPOT spot image. Logistic regression was used to establish prediction model based on CNN. RESULTS: Areas under the receiver operating characteristic curve (AUCs) of early secreted antigenic target 6 (ESAT-6) CNN, culture filtrate protein 10 (CFP-10) CNN, and phytohemagglutinin (PHA) CNN were more than 0...
January 6, 2023: Diagnostic Microbiology and Infectious Disease
Andrea H L Bruning, Maaike van den Beld, Jeroen Laverge, Matthijs R A Welkers, Sacha D Kuil, Sylvia M Bruisten, Alje P van Dam, Arjen J Stam
We present an 18-year-old woman with a urinary tract infection caused by Salmonella Oranienburg. S. Oranienburg was isolated from her pet snake and confirmed as the source of infection using whole genome sequencing. Our case demonstrates the risk of acquiring reptile-associated salmonellosis and stretches the need for awareness to prevent these infections.
January 4, 2023: Diagnostic Microbiology and Infectious Disease
Xueping Ma, Haozhen Yan, Jing Zhang, Cui Zhang, Cuijuan Duan, Shulei Li, Wenfeng Ding, Yingli Li, Wenjing Lu, Yufei Wang, Xiaoli Yang
Golgi protein 73 (GP73) has been recognized as a biomarker for evaluating liver diseases, although the serum profile of patients with HIV remains unclear. This study was designed to investigate the diagnostic values of serum GP73 in patients with HIV. A total of 92 patients with HIV and 60 healthy participants were selected, and serum samples were collected; 51 of 92 patients were followed up and all indicators were re-tested after 1 year. Patients with HIV had significantly lower GP73 concentration, lower viral load, and higher CD4+ T cell counts after antiretroviral treatment...
January 4, 2023: Diagnostic Microbiology and Infectious Disease
Kuenyoul Park, Heungsup Sung, Mi-Na Kim
OBJECTIVES: This study assessed the clinical performance of the cobas Liat SARS‑CoV‑2 & Influenza A/B assay (LiatCOVID/flu) for the detection of both severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and influenza viruses during the SARS-CoV-2 Omicron outbreak. METHODS: Residual nasopharyngeal swab samples (NPS) previously tested with cobas SARS-CoV-2 & Influenza A/B for SARS-CoV-2 and with the Allplex Respiratory Panel 1 for influenza viruses were collected...
January 4, 2023: Diagnostic Microbiology and Infectious Disease
Andressa de Azambuja Pias Weber, Jovana Simonetti Bulegon, Manoela Dias de Souza, Silmara Ana Vendrame, Larissa Venturini, Wendel Mombaque Dos Santos, Thissiane de Lima Gonçalves
This study aimed to profile the clinical progression, demographics, and oxidative status of COVID-19 patients, correlating with disease severity. The study included 143 participants: 93 patients with COVID-19 (28 outpatients, 65 inpatients), and 50 control participants. Thiobarbituric acid reactive substance (TBARS) was used as an oxidative damage marker. Antioxidant activity was assessed via quantification of Vitamin C, sulfhydryl groups, ferric reduction ability of plasma (FRAP), Uric acid (UA), and evaluation of delta-aminolevulinate dehydratase (δ-ALA-D) enzymatic activity...
December 30, 2022: Diagnostic Microbiology and Infectious Disease
Gaetan Gavazzi, Lucile Meyrignac, Nabil Zerhouni, Catherine Bioteau, Clara Lopez, Patrice François, Sabine Drevet
OBJECTIVE: To evaluate PCT measurement in the diagnosis of bloodstream infection (BSI) in hospitalized patients aged 75+. METHOD: Descriptive, retrospective, monocentric study conducted in France, in patients with at least one blood culture and PCT and CRP measurements within the 24 hours before or after blood culture. RESULTS: The mean PCT and CRP values for the 118 (15.2%) positive blood cultures were 18.90 ng/ml [95%CI: 0.007-334.7] and 153...
December 30, 2022: Diagnostic Microbiology and Infectious Disease
Kazufumi Ikuta, Ryoko Kanno, Tomoaki Bessho, Tetsuo Koshizuka, Tatsuo Suzutani
Primary Toxoplasma gondii (T. gondii) infection during pregnancy could result in congenital disease with severe clinical complications. IgM antibodies are one of the indices of primary infection. The IgG avidity index (AI) is also known to remain low for at least 3 months after primary infection. Here, we evaluated and compared the performance of T. gondii IgG avidity assays as confirmed by T. gondii IgM serostatus and number of days post-exposure. Four assays preferentially used in Japan were employed to measure the T...
April 2023: Diagnostic Microbiology and Infectious Disease
Weiwei Su, Liang Ju, Qi Hua, Jing Hu, Wei Qian
This research aimed to explore the clinical value of C-reactive protein (CRP), procalcitonin (PCT), and serum amyloid A (SAA) in early diagnosis of bacterial pneumonia. CRP, PCT, and SAA levels of children with bacterial pneumonia, children with non-bacterial pneumonia, and healthy children were compared. The sensitivity and specificity of CRP, PCT, and SAA in the diagnosis of bacterial pneumonia in children were compared. CRP, PCT, and SAA levels were significantly lower in healthy children when compared with children with Community acquired pneumonia (CAP)...
February 2023: Diagnostic Microbiology and Infectious Disease
Bojana Tešović, Jakov Nišavić, Bojana Banović Đeri, Tamaš Petrović, Andrea Radalj, Milanko Šekler, Kazimir Matović, Zoran Debeljak, Nikola Vasković, Marko Dmitrić, Dejan Vidanović
West Nile virus (WNV) can affect humans, birds, horses and another mammals, causing asymptomatic infection, mild febrile disease, neurological and systematic disease and death. In order to gain insight into the prevalence of WNV, a monitoring program has been established in the Republic of Serbia. Whole genome sequencing is essential for the molecular epizootiological analysis of virus entry and transmission routes, especially in high-risk regions. This paper describes the development of a multiplex PCR based NGS protocol for whole genome sequencing of WNV lineage 2 directly from biological samples using Oxford Nanopore (ONT) platform...
February 2023: Diagnostic Microbiology and Infectious Disease
Alexandria D Lewis, Morgan R Bridwell, Michael D Hambuchen, Tyler B Clay, Kara W Orwig
Methicillin-resistant Staphylococcus aureus (MRSA) is a pathogen empirically covered in pulmonary infections. Limited studies evaluate the relationship between the MRSA PCR nasal swab assays and clinically diagnosed ventilator-associated pneumonia (VAP), lung abscess, and empyema. This retrospective, single-center study included 161 patients, which aimed to validate the clinical utility of MRSA PCR nasal swabs in VAP, lung abscess, and empyema through sensitivity, specificity, positive predictive value (PPV), and negative predicative value (NPV) analysis...
January 2023: Diagnostic Microbiology and Infectious Disease
Toshiharu Sasaki, Yohei Doi
Staphylococcus lugdunensis is exceptionally virulent among the coagulase-negative Staphylococcus species, but the clinical significance of single-positive bacteremia of S. lugdunensis remains uncertain. We investigated S. lugdunensis bacteremia cases over 10 years. Of the 49 cases included, 12 had multiple-positive blood cultures and 37 had single-positive blood cultures. Antimicrobial therapy was given to over 80% of both groups, whereas the duration of therapy was significantly longer in the multiple-positive group...
January 2023: Diagnostic Microbiology and Infectious Disease
Jonathan Binz, Mallory Heft, Samantha Robinson, Hanna Jensen, James Newton
OBJECTIVE: Aspiration can lead to complications such as aspiration pneumonia (ASPNA) or aspiration pneumonitis. Use of procalcitonin (PCT) assays has been supported to help differentiate between bacterial and nonbacterial etiologies for infection. We hypothesize PCT levels will differ significantly in patients with ASPNA versus aspiration pneumonitis. METHODS: This study retrospectively analyzed patients with an ICD-10 diagnosis of ASPNA or aspiration pneumonitis from September 2017 to September 2019...
January 2023: Diagnostic Microbiology and Infectious Disease
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