Xiaodi Qiu, Shaohua Zhang, Youmeng Zhang, Lei Cai, Dan Li, Yi Lu
PURPOSE: To identify the inactive genes in cataract lenses and explore their function in lens epithelial cells (LECs). METHODS: Lens epithelium samples obtained from both age-related cataract (ARC) patients and normal donors were subjected to two forms of histone H3 immunoprecipitation: H3K9ac and H3K27me3 chromatin immunoprecipitation (ChIP), followed by ChIP-seq. The intersection set of "active genes in normal controls" and "repressed genes in cataract lenses" was identified...
January 10, 2024: Current Eye Research
Sara T Nøland, Morten S Magnø, Tor P Utheim, Xiangjun Chen
PURPOSE: In this review, we aimed to investigate the literature on sex-specific prevalence of meibomian gland dysfunction (MGD) and to determine whether women or men are more at risk for MGD. METHODS: A search was conducted on PubMed using the terms: (Sex OR Gender OR prevalence) AND (Meibomian gland) . RESULTS: Twenty-four relevant studies on MGD prevalence were identified, including 10 population-based and 14 hospital-based studies. Among the population-based studies, five studies reported higher rates among men, three studies found no differences, and one study observed higher rates among women...
January 9, 2024: Current Eye Research
Sunghyuk Moon, Joseph Park, Seongjin Lim, Soh Youn Suh, Alan Le, Joseph L Demer
Purpose: We employed automated analysis of scanning laser ophthalmoscopy (SLO) to determine if mechanical strains imposed on disc, and retinal and choroidal vessels during horizontal duction in children differ from those of adults. Methods: Thirty-one children aged 11.3 ± 2.7 (standard deviation) years underwent SLO in central gaze, and 35° ab- and adduction. Automated registration with deep learning-based optical flow analysis quantified vessel deformations as horizontal, vertical, shear, and equivalent strains...
January 7, 2024: Current Eye Research
Keith D Miller, Avi Toiv, Callie Deng, Ming-Chen Lu, Leslie M Niziol, Jenna N Hart, Eric Sherman, Shahzad I Mian, Paul R Lephart, Alan Sugar, Linda Kang, Maria A Woodward
PURPOSE: Negative laboratory results make targeting microbial keratitis treatment difficult. We investigated factors associated with laboratory negativity in patients with microbial keratitis in the context of a transition to a new specimen collection method. METHODS: Microbial keratitis patients with associated laboratory tests were identified in the electronic health record of a tertiary care facility from August 2012 to April 2022. Patient demographics and laboratory results were obtained...
January 5, 2024: Current Eye Research
Yang Lu, Lan Gong, Yue Yin
PURPOSE: To evaluate whether patients with moderate-to-severe meibomian gland dysfunction (MGD) will benefit from increasing the number of intense pulsed light (IPL) treatment sessions. METHODS: Ninety Asian adult with MGD (stages 3-4) were enrolled in this retrospective study. In Group1, 30 patients completed the five-session IPL treatment, 63.33% of which also received meibomian gland expression (MGX). In Group 2, 60 patients received three-session IPL treatment, 60...
January 4, 2024: Current Eye Research
Rui Zhao, Leying Wang, Fei Chen, Hongjuan Liu, Chunli Chen, Jing Zhang, Sitong Guo, Qingfeng Liang, Libin Jiang
PURPOSE: Optic neuritis (ON), a demyelinating disease of the central nervous system, is often a precursor manifestation of neuromyelitis optica spectrum disorders (NMOSD) or multiple sclerosis (MS). Reduced corneal nerve fiber counts have been found in patients with NMOSD or MS. This study aimed to observe and compare the corneal subbasal nerve plexus in patients with three types of ON and controls without ON using in vivo corneal confocal microscopy (IVCM). METHODS: Data were analyzed for 77 eyes of 48 patients with ON, grouped according to seropositivity for anti-aquaporin-4 IgG, myelin oligodendrocyte glycoprotein antibody, or no seropositivity, and 35 healthy eyes in the control group...
January 2, 2024: Current Eye Research
Yu-Hong Cui, Chao-Qun Liu, Xi-Ling Song, Wan-Zhao Yi, Qi Liu, Jing-Min Liu, Ya-Ni Wu, Jian-Ying Chen, Lv-Jun Yang, Hui-Ying He, Jing Meng, Hong-Wei Pan
PURPOSE: Circular RNAs (circRNAs) are products of alternative splicing with roles as competitive endogenous RNAs or microRNA sponges, regulating gene expression and biological processes. However, the involvement of circRNAs in herpes simplex keratitis remains largely unexplored. METHODS: This study examines circRNA and miRNA expression profiles in primary human corneal epithelial cells infected with HSV-1, compared to uninfected controls, using microarray analysis...
January 2, 2024: Current Eye Research
Fulya Yaylacıoğlu Tuncay, Beril Talim, Pervin Rukiye Dinçer
PURPOSE: Mutations in transforming growth factor beta-induced (TGFBI) protein are associated with a group of corneal dystrophies (CDs), classified as TGFBI-associated CDs, characterized by deposits in the cornea. Mouse models were not proper in several aspects for modelling human disease. The goal of this study was to generate zebrafish mutants to investigate the corneal phenotype and to decide whether zebrafish could be a potential model for TGFBI-associated CDs. METHODS: The conserved arginine residue, codon 117, in zebrafish tgfbi gene was targeted with Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 method...
January 2, 2024: Current Eye Research
Zeeshan Patel, Sankalp Gharat, Munira Momin
PURPOSE: Drugs administered in the ocular region need to overcome ocular barriers without permanently damaging the ocular tissues. Moreover, ocular disorders of the posterior segment are more difficult to treat due to invasive procedures required to reach the posterior segment. Hence, to treat posterior disorders of the eye an attempt was made to develop nanofiber (NF) scaffolds for effective management of chronic posterior uveitis. Nanofibers (NFs) were formulated using the electrospinning technique...
January 1, 2024: Current Eye Research
Binyao Chen, Shirong Chen, Jianling Yang, Hongxi Wang, Mingzhi Zhang
PURPOSE: To explore the characteristics and determinants of peripapillary retinal nerve fiber layer (RNFL) optical density (OD) by optical coherence tomography (OCT) in healthy eyes with varied analytical radii. METHODS: Peripapillary OCT scans centered at the optic disc of 150 eyes from 150 healthy subjects (64 males and 86 females) were included. Under 5 analytical circles with different radii (1.45 mm, 1.7 mm, 1.95 mm, 2.2 mm and 2...
December 28, 2023: Current Eye Research
Ana Boto de Los Bueis, Cristina Vidal Arranz, Almudena Del Hierro-Zarzuelo, David Díaz Valle, Rosalía Méndez Fernández, María Isabel Gabarrón Hermosilla, José Manuel Benítez Del Castillo, Mariano García-Arranz
PURPOSE: To determine the safety and feasibility of human autologous adipose tissue-derived adult mesenchymal stem cells (ASCs) for ocular surface regeneration in patients with bilateral limbal stem-cell deficiency (LSCD). METHODS: A phase IIa clinical trial was designed (, NCT01808378) with 8 patients, 3 of whom had aniridia, 2 meibomian glands diseases, 2 multiple surgeries and 1 chronic chemical injury. The therapeutic protocol was as follows: 6-mm of central corneal epithelium was removed, 400,000 ASCs were injected into each limboconjunctival quadrant, 400,000 ASCs were suspended over the cornea for 20 min, and finally the cornea was covered with an amniotic membrane patch...
December 28, 2023: Current Eye Research
Haeyeon Lee, Mandkhai Molomjamts, Heidi Roehrich, Sydney Gudvangen, Chanel Asuncion, Michael K Georgieff, Phu Tran, Linda K McLoon, Ellen C Ingolfsland
PURPOSE: To determine the retinal transcriptomic differences underlying the oxygen-induced retinopathy phenotypes between Sprague Dawley rat pups from two commonly used commercial vendors. This will allow us to discover genes and pathways that may be related to differences in disease severity in similarly aged premature babies and suggest possible new treatment approaches. METHODS: We analyzed retinal vascular morphometry and transcriptomes from Sprague Dawley rat pups from Charles River Laboratories and Envigo (previously Harlan)...
December 28, 2023: Current Eye Research
Fenghua Cui, Zhonghua Sun, Xueyan Zhang, Cuijuan Liu
BACKGROUND: Dysregulated circular RNAs (circRNAs) is involved in the pathogenesis of age-related cataract (ARC). Here, this study aimed to explore the function and mechanism of circMAP3K4 in ARC. METHODS: Human lens epithelial cells were exposed to hydrogen peroxide (H2O2) for functional experiments. qRT-PCR and western blotting analyses were used for the expression detection of genes and proteins. Cell proliferation was tested using cell counting kit-8 and EdU...
December 28, 2023: Current Eye Research
Mohamad Hasan, Scott Joslin, Mike Chemaly, Bailin Liang
PURPOSE: The objective of this study was to quantitatively assess the concentration of human tear proteins in patients wearing contact lenses of various ionicities and determine whether differences were related to the incidence of corneal infiltrative events (CIE). METHODS: 24 subjects (samples) were randomly selected for spectral count analysis to obtain protein concentrations using LCMS analysis. The subjects were neophyte and ametropic with ages between 18 and 40; 6 wore control lenses, 8 wore TestLens1, and 10 wore TestLens2...
December 26, 2023: Current Eye Research
Omar Abdelrahman, Marius Topka, Yao Zhang, Antonia Bock, Johannes Lörner, Rebecca Jungbauer, Thilo Hotfiel, Friedrich Paulsen, Christian M Hammer
PURPOSE: To elucidate whether it is feasible to use porcine eyes from scalded, abattoir-acquired animals for refractive femtosecond laser research. METHODS: An infrared laser (FS 200) and an ultraviolet laser (prototype version) were tested for their applicability on scalded pig eyes. Fifty porcine eyes were divided into two equally-sized groups and assigned to either the infrared or the ultraviolet laser. Both laser groups were comprised of five subgroups of n  = 5 eyes each...
December 26, 2023: Current Eye Research
Heather Heitkotter, Mitchell T Allphin, Ana Untaroiu, Heun Min, Emma Warr, Niamh Wynne, Robert F Cooper, Joseph Carroll
PURPOSE: To compare peak cone density predicted from outer segment length measured on optical coherence tomography with direct measures of peak cone density from adaptive optics scanning light ophthalmoscopy. METHODS: Data from 42 healthy participants with direct peak cone density measures and optical coherence tomography line scans available were used in this study. Longitudinal reflectivity profiles were analyzed using two methods of identifying the boundaries of the ellipsoid and interdigitation zones to estimate maximum outer segment length: peak-to-peak and the slope method...
December 26, 2023: Current Eye Research
Xiang-Ning Wang, Shuting Li, Xuan Cai, Tingting Li, Da Long, Qiang Wu
PURPOSE: To evaluate the prevalence and types of artifacts in ultrawide-field swept-source optical coherence tomography angiography (SS-OCTA) scans of diabetic retinopathy (DR) patients. METHODS: This study was a prospective, observational study conducted from May 2022 to October 2022. Participants comprised individuals with proliferative diabetic retinopathy (PDR), nonproliferative diabetic retinopathy (NPDR), no diabetic retinopathy, and healthy controls. SS-OCTA imaging was performed, and a 5-scan composite with a larger field of view (23...
December 20, 2023: Current Eye Research
Le Zhang, Zi-Feng Zhang, Yan-Nian Hui, Fang He, Xiao-Rong Guan, Jian Zhou
PURPOSE: To observe the effects of oxidative stress on vascular endothelial growth factor (VEGF) and connections of lens epithelial cells. METHODS: Human lens epithelium of patients with age-related cataract (ARC), both SRA01/04 cells and whole mice lens stimulated by H2 O2 were employed. VEGF in human aqueous humor of ARC-patients and the supernatant of SRA01/04 cells was determined by ELISA. The expressions of VEFG in human lens epithelium were detected by immunofluorescence staining...
December 18, 2023: Current Eye Research
Rui Fang, Hai-Long Li, Ning-Xin Lv, Pei-Lin Yue, Yu-Xuan Jia, Zhao-Chuan Liu, Hong-Gang Zhou, Xu-Dong Song
PURPOSE: Accumulated evidence has shown that microRNAs (miRNAs) are closely related to the pathogenesis and progression of senile cataracts. Here we investigate the effect of miR-29a-3p in cataractogenesis and determined the potential molecular mechanism involved. METHODS: In this study, we constructed a selenite cataract model in rats and obtained the miRNAs related to cataracts by whole transcriptome sequencing. To investigate the effect and mechanism of miR-29a-3p on cataracts, we performed several in vivo and in vitro experiments, including CCK8 assay, flow cytometry, luciferase reporter assay, Edu assay, and western blot analysis...
December 14, 2023: Current Eye Research
Sphurti S Adigal, Neetha I R Kuzhuppilly, Nagaraj Hegde, Nidheesh V R, Alisha Rizvi, Reena V John, Sajan D George, Vasudevan B Kartha, Sulatha V Bhandary, Santhosh Chidangil
PURPOSE: The study showcased the application of the lab-assembled HPLC-LED-IF system to analyze proteins in tear fluid samples collected from individuals diagnosed with primary open-angle glaucoma (POAG). METHODS: Clinical application of the said technique was evaluated by recording chromatograms of tear fluid samples from control and POAG subjects and by analyzing the protein profile using multivariate analysis. The data analysis methods involved are principal component analysis (PCA), Match/No-Match, and artificial neural network (ANN) based binary classification for disease diagnosis...
December 11, 2023: Current Eye Research
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