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Veterinary Research Communications

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https://read.qxmd.com/read/30689110/localization-profiles-of-natriuretic-peptides-in-hearts-of-pre-hibernating-and-hibernating-anatolian-ground-squirrels-spermophilus-xanthoprymnus
#1
Mustafa Öztop, Mehmet Özbek, Narin Liman, Feyzullah Beyaz, Emel Ergün, Levent Ergün
The Anatolian ground squirrel (Spermophilus xanthoprymnus) is a typical example of true mammalian hibernators. In order to adapt to extreme external and internal environments during hibernation, they lower their body temperatures, heart rates and oxygen consumption; however, pathological events such as ischemia and ventricular fibrillation do not occur in their cardiovascular systems. During the hibernation, maintenance of cardiac function is very important for survival of ground squirrels. Natriuretic peptides (NPs) are key factors in the regulation of cardiovascular hemostasis...
January 28, 2019: Veterinary Research Communications
https://read.qxmd.com/read/30656543/stem-cells-on-regenerative-and-reproductive-science-in-domestic-animals
#2
REVIEW
Naira Caroline Godoy Pieri, Aline Fernanda de Souza, Ramon Cesar Botigelli, Lucas Simões Machado, Carlos Eduardo Ambrosio, Daniele Dos Santos Martins, André Furugen Cesar de Andrade, Flavio Vieira Meirelles, Poul Hyttel, Fabiana Fernandes Bressan
Stem cells are undifferentiated and self-renewable cells that present new possibilities for both regenerative medicine and the understanding of early mammalian development. Adult multipotent stem cells are already widely used worldwide in human and veterinary medicine, and their therapeutic signalling, particularly with respect to immunomodulation, and their trophic properties have been intensively studied. The derivation of embryonic stem cells (ESCs) from domestic species, however, has been challenging, and the poor results do not reflect the successes obtained in mouse and human experiments...
January 17, 2019: Veterinary Research Communications
https://read.qxmd.com/read/30612297/the-influence-of-dermanyssus-gallinae-and-different-lighting-regimens-on-selected-blood-proteins-corticosterone-levels-and-egg-production-in-layer-hens
#3
Rajmund Sokół, Sylwia Koziatek-Sadłowska, Maria Michalczyk
Egg production in battery cage systems in commercial poultry farms promotes uncontrolled growth of poultry ectoparasite Dermanyssus gallinae. Intermittent lighting regimens provided a promising alternative for controlling D. gallinae invasions. The study analysed the influence of D. gallinae invasions on selected blood protein fractions (albumin, α-, β-, γ-globulin), corticosterone levels and egg production in Hy-Line Brown layer hens exposed to two lighting regimens: A (16 L:8D) and B (intermittent (4 L:2D); L-light, D-dark)...
January 5, 2019: Veterinary Research Communications
https://read.qxmd.com/read/30591981/acinetobacter-baumannii-a-neglected-pathogen-in-veterinary-and-environmental-health-in-germany
#4
REVIEW
Gamal Wareth, Heinrich Neubauer, Lisa D Sprague
The emergence and global spread of drug resistant Acinetobacter (A.) baumannii is a cause of great concern. The current knowledge on antibiotic resistance in A. baumannii from animal origin is mostly based on few internationally published case reports, investigations of strain collections and several whole genome analyses. This lack of data results in a somewhat sketchy picture on how to assess the possible impact of drug resistant A. baumannii strains on veterinary and public health in Germany. Consequently, there is an urgent need to intensify the surveillance of A...
December 27, 2018: Veterinary Research Communications
https://read.qxmd.com/read/30569275/impacts-on-performance-of-growing-finishing-pigs-under-heat-stress-conditions-a-meta-analysis
#5
Angela Cristina da Fonseca de Oliveira, Karoline Vanelli, Cristina Santos Sotomaior, Saulo Henrique Weber, Leandro Batista Costa
High ambient temperatures are a challenge for animal production around the world, and they are one of the major reasons for economic and productive losses in pig production. Under stress conditions, the energy contribution to productive functions is reduced, generating health imbalances, decreased productivity rates and changes in animal behavior. Despite the numerous articles published on this subject, the variability of results on performance parameters is high. For this reason, the objective of the present study was to evaluate the actual impact of high ambient temperature (HAT) (29 °C to 35 °C) on growing-finishing pig performance, compared with animals kept in a thermoneutral environment (TNT) (18 °C to 25 °C), based on meta-analysis...
December 19, 2018: Veterinary Research Communications
https://read.qxmd.com/read/30484171/correction-to-optimised-isolation-method-for-rna-extraction-suitable-for-rna-sequencing-from-feline-teeth-collected-in-a-clinical-setting-and-at-post-mortem
#6
S Lee, U Trivedi, C Johnson, C Farquharson, G T Bergkvist
The authors regret that the original version of the above article contained errors in the Figs. 3, 4 and Tables 3 legends. The errors has been corrected.
November 27, 2018: Veterinary Research Communications
https://read.qxmd.com/read/30238341/could-hypoxia-influence-basic-biological-properties-and-ultrastructural-features-of-adult-canine-mesenchymal-stem-stromal-cells
#7
Eleonora Iacono, Luisa Pascucci, Cinzia Bazzucchi, Marco Cunto, Francesca Ricci, Barbara Rossi, Barbara Merlo
The aim of the present study was to compare canine adipose tissue mesenchymal stem cells cultured under normoxic (20% O2 ) and not severe hypoxic (7% O2 ) conditions in terms of marker expression, proliferation rate, differentiation potential and cell morphology. Intra-abdominal fat tissue samples were recovered from 4 dogs and cells isolated from each sample were cultured under hypoxic and normoxic conditions. Proliferation rate and adhesion ability were determined, differentiation towards chondrogenic, osteogenic and adipogenic lineages was induced; the expression of CD44, CD34, DLA-DQA1, DLA-DRA1 was determined by PCR, while flow cytometry analysis for CD90, CD105, CD45 and CD14 was carried out...
December 2018: Veterinary Research Communications
https://read.qxmd.com/read/30219981/isolation-and-characterization-of-two-lytic-bacteriophages-against-staphylococcus-aureus-from-india-newer-therapeutic-agents-against-bovine-mastitis
#8
M Y Ganaie, S Qureshi, Z Kashoo, S A Wani, M I Hussain, R Kumar, R Maqbool, P Sikander, M S Banday, W A Malla, P Mondal, R I N Khan
Bovine mastitis causes severe economic losses to dairy farmers. Staphylococcus aureus, is one of the most important pathogen implicated in etiology of clinical and subclinical mastitis in bovines. In view of increasing antimicrobial resistance alternatives to antibiotic therapy are much needed. The present decade has witnessed a renewed interest in phage based therapeutics and diagnostics. The present study, describes isolation and characterization of two lytic phages SAJK-IND and MSP against Staphylococcus aureus having a potential to be used in therapy against mastitis...
December 2018: Veterinary Research Communications
https://read.qxmd.com/read/30145726/cell-free-mitochondrial-dna-in-serum-and-milk-associated-with-bovine-mastitis-a-pilot-study
#9
Geeta Devi Leishangthem, Niraj Kumar Singh, Nittin Dev Singh, Gursimran Filia, Amarjit Singh
Mastitis is inflammation of mammary gland affecting all the species of domestic animals. Fragments of the mitochondrial genome released from dying cells are considered surrogate markers of mitochondrial injury. We hypothesized that bovine mastitis would be associated with increased cell free mitochondrial DNA (mtDNA) content in serum and milk. Milk and serum samples were collected from sub-clinical mastitic and normal animals. Mastitis was confirmed by California mastitis test and bacterial isolation. Oxidative stress, nitric oxide and inflammatory cytokines were also estimated...
December 2018: Veterinary Research Communications
https://read.qxmd.com/read/30097755/triggers-of-nlrc4-and-aim2-inflammasomes-induce-porcine-il-1%C3%AE-secretion
#10
Huijeong Ahn, Jeongeun Kim, Sungkyun Kwon, Pyeung-Hyeun Kim, Hyuk Moo Kwon, Eunsong Lee, Geun-Shik Lee
Pigs are an important livestock and serve as a large animal model due to physiological and anatomical similarities with humans. Thus, components of the porcine immune system such as inflammasomes need to be characterized for disease control, vaccination, and translational research purposes. Previously, we and others elucidated porcine nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) family Pyrin domain containing 3 (NLRP3) inflammasome activation. However, until now, porcine NLR family caspase recruitment domain (CARD)-containing 4 (NLRC4) and absent in melanoma 2 (AIM2) inflammasomes have been not well studied...
December 2018: Veterinary Research Communications
https://read.qxmd.com/read/30094586/the-impdh-inhibitors-ribavirin-and-mycophenolic-acid-inhibit-peste-des-petits-ruminants-virus-infection
#11
LETTER
Qiu-Yan Chang, Fu-Cheng Guo, Xue-Rui Li, Jian-Hua Zhou, Xuepeng Cai, Qiuwei Pan, Xiao-Xia Ma
Peste des petits ruminants virus (PPRV) causes highly contagious diseases in domestic and particular wild small ruminants, leading to substantial economic loss. The development of effective and cheap antiviral medications shall help to circumvent this emerging burden. In this study, we found that ribavirin, a competitive inosine-5'-monophosphate dehydrogenase (IMPDH) inhibitor, significantly inhibits the replication of PPRV. As IMPDH is a key enzyme in purine nucleotide synthesis, supplementation of exogenous guanosine attenuate the anti-PPRV effect of ribavirin...
December 2018: Veterinary Research Communications
https://read.qxmd.com/read/30402716/optimised-isolation-method-for-rna-extraction-suitable-for-rna-sequencing-from-feline-teeth-collected-in-a-clinical-setting-and-at-post-mortem
#12
S Lee, U Trivedi, C Johnson, C Farquharson, G T Bergkvist
Advanced next generation sequencing approaches have started to reveal the cellular and molecular complexity of the microenvironment in many tissues. It is challenging to obtain high quality RNA from mineralised tissues. We developed an optimised method of RNA extraction from feline teeth collected in a clinical setting and at post mortem. Teeth were homogenised in phenol-guanidinium solution at near-freezing temperatures and followed by solid-phase nucleic acid extraction utilising a commercially available kit...
November 6, 2018: Veterinary Research Communications
https://read.qxmd.com/read/30187173/melanocortin-2-receptor-antagonists-in-canine-pituitary-dependent-hypercortisolism-in-vitro-studies
#13
Karin Sanders, Jan A Mol, Hans S Kooistra, Sara Galac
Canine hypercortisolism is most often caused by an ACTH-secreting pituitary adenoma (pituitary-dependent hypercortisolism; PDH). An interesting target for a selective medical treatment of PDH would be the receptor for ACTH: the melanocortin 2 receptor (MC2R). In this study we investigated whether two peptide compounds, BIM-22776 (#776) and BIM-22A299 (#299), are effective MC2R antagonists in vitro. Their effects on cortisol production and mRNA expression of steroidogenic enzymes, MC2R and melanocortin 2 receptor accessory protein (MRAP) were evaluated in primary adrenocortical cell cultures (n = 8) of normal canine adrenal glands...
September 5, 2018: Veterinary Research Communications
https://read.qxmd.com/read/30083868/correction-to-the-synergistic-effect-of-organic-acids-phytochemicals-and-a-permeabilizing-complex-reduces-salmonella-typhimurium-1-4-5-12-i-shedding-in-pigs
#14
Jessica Ruggeri, Fabio Foresti, Roberta Pavesi, Alessia Terrini, Francesca Giudici, Diego Padoan, Attilio Corradi, Maria Cristina Ossiprandi, Paolo Pasquali, Giovanni Loris Alborali
This article was originally published with all author names incorrectly listed. All author names have now been transposed and appear correctly above.
September 2018: Veterinary Research Communications
https://read.qxmd.com/read/30069619/a-porcine-cholecyst-derived-scaffold-for-treating-full-thickness-lacerated-skin-wounds-in-dogs
#15
RANDOMIZED CONTROLLED TRIAL
Satheesan Karthika, Sainulabdeen Anoop, C B Devanand, M K Narayanan, Madhavan Unni, Saji Eassow, Thapasimuthu Anilkumar
In regenerative medicine, despite the chances of graft-rejection, scaffolds prepared from extracellular matrices of various mammalian organs/tissues are widely used. Graft-assisted healing of full thickness skin-wounds is a major use of these bioscaffolds. Therefore, considering its prospective clinical use as a wound healing matrix, this study evaluated the healing potential of porcine cholecyst-derived scaffold (CDS) prepared by a non-detergent/enzymatic method for treating naturally occurring full thickness lacerated wounds in dogs...
September 2018: Veterinary Research Communications
https://read.qxmd.com/read/30043292/antibiotic-susceptibility-of-staphylococcus-aureus-isolated-from-subclinical-bovine-mastitis-cases-and-in-vitro-efficacy-of-bacteriophage
#16
RANDOMIZED CONTROLLED TRIAL
Diego F Varela-Ortiz, José E Barboza-Corona, Joaquín González-Marrero, Ma Fabiola León-Galván, Mauricio Valencia-Posadas, Alma A Lechuga-Arana, Cynthia G Sánchez-Felipe, Fernanda Ledezma-García, Abner J Gutiérrez-Chávez
Staphylococcus aureus is an opportunistic pathogen that may cause severe infections in livestock, and represents the major cause of mastitis in dairy cows. Currently, instead of using antibiotics, new strategies are sought to reduce this clinical health problem. The aim of this study was to determine the efficacy of phage therapy to kill S. aureus strains obtained from farms located at the State of Guanajuato, México. Thirty-six S. aureus strains from cow milk with subclinical mastitis were isolated and identified, and the susceptibility to antibiotics and four phages also isolated in this work was tested...
September 2018: Veterinary Research Communications
https://read.qxmd.com/read/29790058/the-synergistic-effect-of-organic-acids-phytochemicals-and-a-permeabilizing-complex-reduces-salmonella-typhimurium-1-4-5-12-i-shedding-in-pigs
#17
RANDOMIZED CONTROLLED TRIAL
Jessica Ruggeri, Fabio Foresti, Roberta Pavesi, Alessia Terrini, Francesca Giudici, Diego Padoan, Attilio Corradi, Maria Cristina Ossiprandi, Paolo Pasquali, Giovanni Loris Alborali
Salmonella Typhimurium (including S.Typhimurium 1,4,[5],12:i-) and other enteric pathogens cause acute infection in pigs during the weaning stage, often evolving into chronic infections responsible for the introduction of zoonotic bacteria into the slaughterhouse and thus determining carcass contamination. In addition to being zoonotic hazards, these pathogens are responsible for economic losses in affected farms. Traditionally, antibiotic treatments have been largely administered in order to reduce the infection burden but it favored, as a direct consequence, an increase in the number of multi-drug resistance strains...
September 2018: Veterinary Research Communications
https://read.qxmd.com/read/29777375/a-comparison-of-the-effects-of-antibiotics-probiotics-synbiotics-and-prebiotics-on-the-performance-and-carcass-characteristics-of-broilers
#18
Vahid Tayeri, Alireza Seidavi, Leila Asadpour, Clive J C Phillips
Routine use of the antibiotic flavomycin in broiler production may lead to resistance, and alternative growth promoters are used to enhance performance. Two hundred day-old male Ross 308 broiler chicks were allocated to five dietary supplements included from d 1-42: flavomycin, three possible alternatives, a probiotic, prebiotic and a synbiotic, as well as a control treatment. There were four replicate cages of 10 birds each in each treatment. Compared with the control and antibiotics treatments, the probiotic, prebiotic and synbiotic treatments increased (p = 0...
September 2018: Veterinary Research Communications
https://read.qxmd.com/read/29721754/effects-of-three-dimensional-spheroid-culture-on-equine-mesenchymal-stem-cell-plasticity
#19
Mi Jeong Park, Jienny Lee, Jeong Su Byeon, Da-Un Jeong, Na-Yeon Gu, In-Soo Cho, Sang-Ho Cha
Mesenchymal stem cells (MSCs) are useful candidates for tissue engineering and cell therapy fields. We optimize culture conditions of equine adipose tissue-derived MSCs (eAD-MSCs) for treatment of horse fractures. To investigate enhancing properties of three-dimensional (3D) culture system in eAD-MSCs, we performed various sized spheroid formation and determined changes in gene expression levels to obtain different sized spheroid for cell therapy. eAD-MSCs were successfully isolated from horse tailhead. Using hanging drop method, spheroid formation was generated for three days...
September 2018: Veterinary Research Communications
https://read.qxmd.com/read/29700691/-nano-immuno-test-for-the-detection-of-live-mycobacterium-avium-subspecies-paratuberculosis-bacilli-in-the-milk-samples-using-magnetic-nano-particles-and-chromogen
#20
Manju Singh, Shoor Vir Singh, Saurabh Gupta, Kundan Kumar Chaubey, Bjorn John Stephan, Jagdip Singh Sohal, Manali Dutta
Early rapid detection of Mycobacterium avium subspecies paratuberculosis (MAP) bacilli in milk samples is the major challenge since traditional culture method is time consuming and laboratory dependent. We report a simple, sensitive and specific nano-technology based 'Nano-immuno test' capable of detecting viable MAP bacilli in the milk samples within 10 h. Viable MAP bacilli were captured by MAP specific antibody-conjugated magnetic nano-particles using resazurin dye as chromogen. Test was optimized using true culture positive (10-bovine and 12-goats) and true culture negative (16-bovine and 25-goats) raw milk samples...
September 2018: Veterinary Research Communications
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