Fabio Suarez-Trujillo, Gilberto Vargas-Alarcon, Ignacio Juarez, Roberto Gil-Martin, Julio Granados, Christian Vaquero-Yuste, Jose Manuel Martin-Villa, Antonio Arnaiz-Villena
Nahua population (also named Aztec or Mexica) was studied for HLA class II genes in a Mexican rural city (Santo Domingo Ocotitlan, Morelos State) belonging to the nowadays Náhuatl speaking areas in Mexico. The most frequent HLA class II alleles were typical Amerindian (HLA-DRB1*04:07, DQB1*03:01 DRB1*04:03 or DRB1*04:04) and also were some calculated extended haplotypes (HLA-DRB1*04:07-DQB1*03:02,DRB1*08:02-DQB1*04:02, or DRB1*10:01-DQB1*05:01 among others). When using HLA-DRB1 Neís genetic distances, our isolated Nahua population was found to be close to other Central America Amerindians like the ancient-established Mayans or Mixe...
March 25, 2023: Human Immunology
Ashwin Ajith, Kenza Mamouni, Abu Musa, Daniel D Horuzsko, Imran Gani, Laura L Mulloy, Anatolij Horuzsko
Despite the growing interest in the role of regulatory B cells (Bregs) in autoimmunity, their distinct role and function in kidney transplant outcomes remain elusive. Here, we retrospectively analyzed the proportion of Bregs, transitional Bregs (tBregs) and memory Bregs (mBregs) and their capacity to produce IL-10 in non-rejected (NR) versus rejected (RJ) kidney transplant recipients. In the NR group, we observed a significant increase in the proportion of mBregs (CD19+ CD24hi CD27+ ) but no difference in tBregs (CD19+ CD24hi CD38+ ), as compared to the RJ group...
March 16, 2023: Human Immunology
Panagiotis Parsonidis, Alexandra Mamagkaki, Ioannis Papasotiriou
Patients with advanced stage breast cancer need novel therapies. New potential treatments have been developed, such as adoptive cellular therapies and alternative cell-free immunotherapies. The goal of this study was to assess the cytotoxicity of three of the patient-derived immune components, CTLs, NK cells and NK-derived EVs, and evaluate the potential for the development of novel therapy against breast cancer. CTLs were activated against MUC-1 antigen. The in vitro cytotoxic activity of three components was assessed with flow cytometry and in vivo study revealed the efficacy of adoptive cell therapy...
March 14, 2023: Human Immunology
Hana Rohn, Sabine Schramm, Krystallenia Pansikaki, Sarah Jansen, Celina Hendriks, Maximilian Platte, Margarethe J Konik, Sebastian Dolff, Benjamin Wilde, Lambros Kordelas, Mirko Trilling, Adalbert Krawczyk, Peter A Horn, Oliver Witzke, Vera Rebmann
The immunosuppressive non-classical human leukocyte antigen-G (HLA-G) can elicits pro-viral activities by down-modulating immune responses. We analysed soluble forms of HLA-G, IL-6 and IL-10 as well as on immune effector cell expression of HLA-G and its cognate ILT-2 receptor in peripheral blood obtained from hospitalised and convalescent COVID-19 patients. Compared with convalescents (N = 202), circulating soluble HLA-G levels (total and vesicular-bound molecules) were significantly increased in hospitalised patients (N = 93) irrespective of the disease severity...
March 14, 2023: Human Immunology
Fabio Suarez-Trujillo, Ignacio Juarez, María José Recio-Hoyas, Diego Rey, José Palacio-Gruber, Roberto Gil-Martin, José Manuel Martín-Villa, Antonio Arnaiz-Villena
HLA-DMB allele frequencies and HLA-DBM-DRB1-DQB1 extended haplotypes were studied for the first time in Amerindians (Cuenca city area, Ecuador). It was found that most common extended haplotypes gathered the most frequent HLA-DRB1 Amerindian alleles. HLA-DMB polymorphism studies may be important to uncover HLA and diseases pathogenesis and also in an extended HLA haplotype frameshift. HLA-DM molecule has a crucial role together with CLIP protein in HLA class II peptide presentation. HLA extended haplotypes including complement and non classical genes alleles are proposed to HLA and disease studies...
March 2, 2023: Human Immunology
Félix Sánchez-Valverde, Eva Martínez-Ojinaga, Ester Donat, Andrés Bodas, Eva Bandrés, Ricardo Torres, Berta Ibáñez, Mª Luz Cilleruelo, Gemma Castillejo, David Pérez-Solis, Carlos Ochoa, Francisco Javier Eizaguirre, Salvador García, Jose Ignacio García, Josefa Barrio, Raquel Vecino, María Del Carmen Miranda, Mercedes Juste, Jose Carlos Salazar, Honorio Armas, Luis Ortigosa, Pedro Urruzuno, Zuriñe García, Elena Balmaseda, Cecila Martínez, Enrique La Orden, Pilar Codoñer, Amadeu Roca, Carlos Trillo, Mercedes Sebastian, Ruth García, Luis Peña-Quintana, Patricia Barros, Marta Soria, Roger García, Begoña Pérez-Moneo, Isabel Polanco, Carmen Ribes, Enriqueta Román
Celiac disease is strongly associated with HLA DQ, specifically with haplotypes. DRB1*03-DQA1*05:01/DQB1*02:01 (DQ2.5),DRB1*07-DQA1*02:01/DQB1*02:02 (DQ2.2), DRB1*11-DQA1*05:05/DQB1*03:01 (DQ7.5), and DRB1*04-DQA1*03:01/DQB1*03:02 (DQ8). The distribution of these risk haplotypes in patients with celiac disease is different in the geographical areas investigated. A high frequency of DRB1*07- DQA1*02:01/DQB1*02:02 (DQ2.2) and DRB1*11-DQA1*05:05/DQB1*03:01 (DQ7.5), has been described in Southern Europe. We analyzed 2102 confirmed CD cases with information on both DQB1* alelles and their distribution by geographical area in Spain...
February 27, 2023: Human Immunology
Garrett R Roll, Robert A Bray, Matthew Cooper, Todd N Eagar, Howard M Gebel, Gayle M Vranic, Kelley M K Hitchman, Julie Houp, Malek Kamoun, John Killian, Jim Kim, Vineeta Kumar, Matthew Levine, Brendan P Lovasik, Tyler Lunow-Luke, Ronald F Parsons, Vikram Pattanayak, Daniel Ranch, Anushi Shah, Peter G Stock, Olga A Timofeeva, Jennifer Trofe-Clark, Chelsey Wongjirad, Heidi Yeh, Stephanie Yi, Raja Rajalingam
Although rare, infection and vaccination can result in antibodies to human leukocyte antigens (HLA). We analyzed the effect of SARS-CoV-2 infection or vaccination on HLA antibodies in waitlisted renal transplant candidates. Specificities were collected and adjudicated if the calculated panel reactive antibodies (cPRA) changed after exposure. Of 409 patients, 285 (69.7 %) had an initial cPRA of 0 %, and 56 (13.7 %) had an initial cPRA > 80 %. The cPRA changed in 26 patients (6...
February 23, 2023: Human Immunology
Jairo Eduardo Niño Ramírez, Antonio Balas, Francisco Javier Gil-Etayo, Isabel Jiménez Hernaz, Pilar Terradillos Sánchez, Ariadna Vicente Parra, Ana Balanzategui, Miguel Alcoceba, Ramón García Sanz, Amalia Tejeda Velarde
The failure to identify HLA null alleles in bone marrow transplantation could be life-threatening because this could result in an HLA mismatch with the ability to trigger the graft-vs-host disease (GVHD) and to reduce patient's survival. In this report we describe the identification and characterization of the novel HLA-DPA1*02:66:02N allele with a non-sense codon in exon 2. This new allele was discovered in two unrelated bone marrow donors during routine HLA-typing using next-generation sequencing (NGS). DPA1*02:66:02N is homologous to DPA1*02:01:01:03 with a single nucleotide difference in exon 2, codon 50, where the replacement of C located at genomic position 3825 by T, causes the formation of a premature stop codon (TGA), resulting in a null allele...
February 14, 2023: Human Immunology
Ehteramolsadat Hosseini, Arefeh Minagar, Mehran Ghasemzadeh, Ali Arabkhazaeli, Alireza Ghasemzadeh
BACKGROUND: HLA-E interaction with inhibitory receptor, NKG2A attenuates NK-mediated cytotoxicity. NKG2A overexpression by SARS-CoV-2 exhausts NK cells function, whereas virus-induced down-regulation of MHC-Ia reduces its derived-leader sequence peptide levels required for proper binding of HLA-E to NKG2A. This leads HLA-E to become more complex with viral antigens and delivers them to CD8+ T cells, which facilitates cytolysis of infected cells. Now, the fact that alleles of HLA-E have different levels of expression and affinity for MHC Ia-derived peptide raises the question of whether HLA-E polymorphisms affect susceptibility to COVID-19 or its severity...
February 13, 2023: Human Immunology
Qingyong Xu, Kurt P Johnson, Maura Hardiman, Dennis Helmick, Adriana Zeevi
Autoantibodies to Angiotensin II type 1 receptor (AT1R) are associated with detrimental outcomes in organ transplants. However, reports showed that adsorption with latex beads reduced positive anti-AT1R antibodies, suggesting possible false reactivity. To investigate this conundrum, we studied 11 samples positive for AT1R antibodies with an ELISA kit before and after adsorption. Adsorption significantly reduced the measurable level of AT1R antibodies (28.3 ± 9.8 vs. 6.3 ± 3.0 U/ml, p < 0...
February 8, 2023: Human Immunology
Omer Faruk Akcay, Haci Hasan Yeter, Yasemin Unsal, Emre Yasar, Sevim Gonen, Ulver Derici
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection could present in a clinical spectrum of varying severity. Human leukocyte antigen (HLA) is a crucial component of the viral antigen presentation pathway and immune response to the virus. Therefore, we aimed to assess the impact of HLA allele polymorphisms on the susceptibility to SARS-CoV-2 infection and related mortality in Turkish kidney transplant recipients and wait listed patients, along with clinical characteristics of the patients...
February 6, 2023: Human Immunology
Abdollah Jafarzadeh, Nasrin Bazargan, Nazanin Chatrabnous, Sara Jafarzadeh, Maryam Nemati
In addition to malignancies, survivin (a member of the apoptosis inhibitor family) has been implicated in the pathogenesis of inflammatory disorders, including autoimmune and allergic diseases. Survivin is constantly expressed in the proliferating hematopoietic progenitor cells, and it is re-expressed in the mature cells of the innate and adaptive immunity, upon activation. Survivin enhances the expression of co-stimulatory molecules and MHC class II molecules in dendritic cells, and promotes the lifespan of macrophages, neutrophils, and eosinophils, while suppressing natural killer (NK) cell activity...
February 6, 2023: Human Immunology
Thao Phuong Bui, Linh Thi Tu Nguyen, Phuong Lan Le, Nhan Thi Thanh Le, Thuy Doan Nguyen, Lieu Van Nguyen, Anh Thi Van Nguyen, Thai Hong Trinh
Several studies have reported an association between certain human leukocyte antigen (HLA) alleles and carbamazepine (CBZ)-induced hypersensitivity reactions in patients with epilepsy. Here, the relationship between the clinical spectrum and the HLA allele profiles in patients with CBZ-induced hypersensitivity reactions was investigated using next-generation sequence (NGS) data obtained from 65 Vietnamese patients with epilepsy, including 33 with CBZ-tolerance and 32 patients with CBZ-hypersensitivity, in which only 8 with severe cutaneous adverse drug reactions and 24 were mild-hypersensitive patients...
January 30, 2023: Human Immunology
Cinthia C Alves, Thaís Arns, Maria L Oliveira, Philippe Moreau, Dinler A Antunes, Erick C Castelli, Celso T Mendes-Junior, Silvana Giuliatti, Eduardo A Donadi
We took advantage of the increasingly evolving approaches for in silico studies concerning protein structures, protein molecular dynamics (MD), protein-protein and protein-DNA docking to evaluate: (i) the structure and MD characteristics of the HLA-G well-recognized isoforms, (ii) the impact of missense mutations at HLA-G receptor genes (LILRB1/2), and (iii) the differential binding of the hypoxia-inducible factor 1 (HIF1) to hypoxia-responsive elements (HRE) at the HLA-G gene. Besides reviewing these topics, they were revisited including the following novel results: (i) the HLA-G6 isoforms were unstable docked or not with β2 -microglobulin or peptide, (ii) missense mutations at LILRB1/2 genes, exchanging amino acids at the intracellular domain, particularly those located within and around the ITIM motifs, may impact the HLA-G binding strength, and (iii) HREs motifs at the HLA-G promoter or exon 2 regions exhibiting a guanine at their third position present a higher affinity for HIF1 when compared to an adenine at the same position...
January 27, 2023: Human Immunology
Velizar Shivarov, Gergana Tsvetkova, Evgueniy Hadjiev, Milena Ivanova
In the present study we analyzed the relevance of HLA class II in JAK2 V617F-positive (JAK2 V617F+) myeloproliferative neoplasms (MPNs) focusing on genotype diversity, associations with specific alleles and haplotypes and the level of gene expression. One hundred and thirty-nine JAK2 V617F+ MPN patients and 1083 healthy controls, typed by Next generation sequencing (NGS) were included in the study. Multivariate generalized linear models with age as a covariate were applied for analysis of HLA-II allele and haplotype associations...
January 25, 2023: Human Immunology
Pramath Kakodkar, Pouneh Dokouhaki, Fang Wu, Jay Shavadia, Revathi Nair, Destinie Webster, Terry Sawyer, Tao Huan, Ahmed Mostafa
AIMS: The HLA system has been implicated as an underlying determinant for modulating the immune response to SARS-CoV-2. In this study, we aimed to determine the association of patients' HLA genetic profiles with the disease severity of COVID-19 infection. METHODS: Prospective study was conducted on COVID-19 patients (n = 40) admitted to hospitals in Saskatoon, Canada, between March and December 2020. Next-generation sequencing was performed on the patient samples to obtain high-resolution HLA typing profiles...
January 20, 2023: Human Immunology
P J S Mendonça-Mattos, L J B Cabral, V H Souza, E S M Filho, A C L Oliveira, I T M M Chaves, D C R O Fernandes, P C M Vieira, M C M Gama, E V O Jorge, H S Anijar, N N Farias, M I P Passos, T R D C Almeida, E B E Santos, M K Palmeira, L M C M Pereira, J F Guerreiro
No abstract text is available yet for this article.
January 12, 2023: Human Immunology
Zahra Sardarmelli, Vida Sheikh, Ghasem Solgi, Mahdi Behzad
OBJECTIVE: The production of interleukin (IL)-29 andthe genes related to IL-29 signaling pathway (STAT1, NF-κB, and NFATc1), and T helper (Th) 1 cells (T-bet, IFN-γ, TNF-α, and IL-2) were evaluated in type 2 diabetes mellitus (T2DM). Correlations between IL and 29 and diabetes parameters, and between gene expression in IL-29 pathway and Th1 cells were also examined. MATERIALS AND METHODS: 41 newly diagnosed patients with T2DM and 41 healthy controls were recruited...
January 10, 2023: Human Immunology
Estella de Jong, Koen D Quint, Abdoelwaheb El Ghalbzouri, Robert M Verdijk, Jelle J Goeman, Sebastiaan Heidt, Frans H J Claas, Johan W de Fijter, Roel E Genders, Maarten H Vermeer, Jan Nico Bouwes Bavinck
BACKGROUND: Solid organ-transplant recipients (SOTR) have an increased risk of cutaneous squamous-cell carcinoma (cSCC), metastasis and death from cSCC. In immunocompetent patients with mucosal SCC, downregulation of HLA class I is associated with poor prognosis. Since the degree of HLA expression on tumor cells could play a role in immunogenicity and pathophysiology of cSCC metastasis, we hypothesized that decreased HLA expression is associated with an increased risk of metastasis. METHODS: We compared HLA expression between primary metastasized cSCCs, their metastases, and non-metastasized cSCCs from the same patients...
January 6, 2023: Human Immunology
So Yeon Kim, Yeseul Kim, Su-Hyun Kim, Sang-Min Han, Hyewon Park, Rosah May Payumo, Ha Eun Kim, Jae-Won Hyun, Ki Hoon Kim, Eun Jig Lee, Ho Jin Kim
Multiple sclerosis (MS) is a demyelinating disease caused by auto-antigen recognizing CD4+ T cells. However, IL-17A-producing CD4+ T cells that are bystander-activated by IL-1β and IL-23, and T cell receptors independently, could contribute to experimental autoimmune encephalomyelitis. Here, we studied the differences in the frequency and function of bystander-activated CD4+ T cells in patients with MS. A significantly higher frequency of CD4 + IL-1Rl + T cells was found in memory than in naïve CD4+ T cells and in Th17/Th17...
January 4, 2023: Human Immunology
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