Alexey S Kazakov, Victoria A Rastrygina, Alisa A Vologzhannikova, Marina Y Zemskova, Lolita A Bobrova, Evgenia I Deryusheva, Maria E Permyakova, Andrey S Sokolov, Ekaterina A Litus, Marina P Shevelyova, Vladimir N Uversky, Eugene A Permyakov, Sergei E Permyakov
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a pleiotropic myelopoietic growth factor and proinflammatory cytokine, clinically used for multiple indications and serving as a promising target for treatment of many disorders, including cancer, multiple sclerosis, rheumatoid arthritis, psoriasis, asthma, COVID-19. We have previously shown that dimeric Ca2+ -bound forms of S100A6 and S100P proteins, members of the multifunctional S100 protein family, are specific to GM-CSF. To probe selectivity of these interactions, the affinity of recombinant human GM-CSF to dimeric Ca2+ -loaded forms of 18 recombinant human S100 proteins was studied by surface plasmon resonance spectroscopy...
March 5, 2024: Cell Calcium