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Experimental Lung Research

Yu-Qing Pan, A-Na Hou
Bronchopulmonary dysplasia (BPD) is a common, chronic lung disease of infants. Presently, high oxygen exposure and mechanical ventilation considerably influence the development of BPD. To clarify the pathological mechanisms of this disease, we developed a hyperoxia-induced BPD rat model and investigated the role of CDKN1A in the pathogenesis of BPD. Newborn rats were randomly assigned to the hyperoxia (85% O2 ) and control (normoxia, 21% O2 ) groups. Lung tissues were collected on days 1, 3, 7, 14, or 21 after the start of hyperoxia or normoxia exposure...
February 12, 2019: Experimental Lung Research
Yongsheng Wang, Yinliang Qi, Xiang Wei, Shaolong Chen, Ning Jia, Qing Zhou, Sumei Zhang, Shuyu Gui, Yuan Wang
Hypoxia induces vigorous growth and a higher malignant phenotype in solid tumors. Hyperoxic treatment using hyperbaric oxygen (HBO) has previously been shown as a highly effective method to attenuate hypoxia. We aimed to investigate the effect of HBO on hypoxia-induced malignancy of lung cancer cells. Cobalt chloride (CoCl2 ) was used to induce chemical hypoxia in lung cancer cell line A549. Hypoxic inducible factor-1α (HIF-1α) expression, lactate dehydrogenase (LDH) activity, migration and invasion capacity, expression profiles of epithelial-mesenchymal transition (EMT) markers and apoptotic markers were assessed in CoCl2 -treated A549 cells, with or without HBO treatment...
February 10, 2019: Experimental Lung Research
Jie Qiu, Ya-Nan Zhang, Xiwei Zheng, Peng Zhang, Gang Ma, Hai Tan
AIM: Klotho expression significantly declines in alveolar macrophages and airway epithelial cells in chronic obstructive pulmonary disease (COPD) patients, and cigarette smoke extract dramatically inhibits the expression and secretion of α-Klotho. This suggests that the silencing of Klotho is the major factor promoting COPD related inflammatory responses. This study aims to investigate the mechanism of Klotho downregulation and its effect on the inflammatory cytokines secretion and cell apoptosis...
January 26, 2019: Experimental Lung Research
Kei Matsuno, Norihiro Harada, Sonoko Harada, Tomohito Takeshige, Ayako Ishimori, Yukinari Itoigawa, Yoko Katsura, Yuzo Kodama, Fumihiko Makino, Jun Ito, Ryo Atsuta, Hisaya Akiba, Kazuhisa Takahashi
AIM OF THE STUDY: In patients with asthma, chronic inflammatory processes and the subsequent remodeling of the airways contribute to the symptoms and the pathophysiological changes. Epithelial-mesenchymal transition (EMT) is thought to play an important role in tissue remodeling. Previous reports show that tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) is a cytokine of the TNF superfamily, exerts pro-inflammatory effects, and enhances transforming growth factor (TGF)-β-induced EMT in bronchial epithelial cells...
January 24, 2019: Experimental Lung Research
Toshiya Inui, Masato Watanabe, Keitaro Nakamoto, Mitsuru Sada, Aya Hirata, Masuo Nakamura, Kojiro Honda, Yukari Ogawa, Saori Takata, Takuma Yokoyama, Takeshi Saraya, Daisuke Kurai, Hiroo Wada, Haruyuki Ishii, Hajime Takizawa
RATIONALE: Neutrophilic airway inflammation plays a central role in chronic obstructive pulmonary disease (COPD). CXC chemokine ligand (CXCL)1 is a neutrophil chemokine involved in the pathogenesis of COPD. However, its clinical significance in COPD patients is poorly understood. AIM OF THE STUDY: To assess the production of CXCL1 by bronchial epithelial cells in response to lipopolysaccharide (LPS) and tumor necrosis factor (TNF)α. MATERIALS AND METHODS: We measured sputum CXCL1 and CXCL8 levels in patients with COPD, asthma, and asthma-COPD overlap (ACO), and compared them to those of patients with interstitial pneumonia (IP)...
January 24, 2019: Experimental Lung Research
Ewelina Wawryk-Gawda, Katarzyna Chłapek, Michał K Zarobkiewicz, Marta Lis-Sochocka, Patrycja Chylińska-Wrzos, Anna Boguszewska-Czubara, Mirosław A Sławiński, Anna Franczak, Barbara Jodłowska-Jędrych, Grażyna Biała
INTRODUCTION: Nicotine stimulates fibroblast proliferation while increasing inflammation and fibrosis of tissues. The cannabinoid receptor 1 (CB1R) is mainly located in the CNS, while cannabinoid receptor 2 (CB2R) is located in the immune cells within the body. CB2R regulates inflammatory processes and fibroblast function. PURPOSE: We investigated the impact of CB2R agonist, JWH 133 and the antagonist, AM630 on lung tissue, applied directly before nicotine application...
January 24, 2019: Experimental Lung Research
Peter Riddell, Jennifer L Gilbert, Emer L Molloy, Shane Finnegan, Jim J Egan, Shirley O'Dea
AIM OF THE STUDY: Nucleic acid-based therapies have the potential to provide clinically meaningful benefit across a wide spectrum of lung disease. However, in vivo delivery remains a challenge. Here we examined the feasibility of using electrospray to deliver nucleic acids to both porcine tracheal tissue sections and whole lung ex vivo. MATERIALS AND METHODS: The effect of electrospray solution, emitter gauge, flow rate and voltage on plasmid DNA integrity was examined by analyzing supercoiled:open circle structure ratio by gel electrophoresis...
January 24, 2019: Experimental Lung Research
Jun-Tao Hu, Jie Lai, Wei Zhou, Xian-Feng Chen, Chi Zhang, Yi-Ping Pan, Liang-Yan Jiang, Yun-Xia Zhou, Bing Zhou, Zhan-Hong Tang
Acute lung injury (ALI) is a common clinical syndrome in ICU departments with high mortality. The pathology of ALI is still not clear and there is no specific and efficient treatment against ALI. In this study, we established ALI rat model through lipopolysaccharide administration. We found that hypothermia therapy led to significant improvement in oxygenation index, edema formation and pathological score, demonstrating that hypothermia is beneficial to the recovery of lung function and alleviation of lung injury...
January 19, 2019: Experimental Lung Research
Hong Zhang, Yuanchun Sun, Wei Rong, Linxia Fan, Yufeng Cai, Qiang Qu, Yun Gao, Hongxia Zhao
AIM OF THE STUDY: To investigate the role of microRNA-221 (miR-221) in the airway epithelial cell injury in asthma and delineate the underlying mechanism that may involve with SIRT1. MATERIALS AND METHOD: Bronchial epithelial cells from asthma patients and healthy controls were obtained by bronchoscopic brushing. The miR-221 and SIRT1 mRNA level in collected cells were detected by qRT-CPR. BEAS2B cell lines were cultured in vitro. In order to up-regulate miR-221 and SIRT1, miR-221 mimic and pcDNA3...
January 17, 2019: Experimental Lung Research
Alan Poisner, Devin Bass, Amanda Fletcher, Ashwin Jain, Janessa Pennington England, Mariah Gawlik Davis, Dauod Arif, Agostino Molteni
PURPOSE: In a model of fat embolism using triolein-treated rats, we have reported that the acute pulmonary histopathological changes at 48 hrs were ameliorated by the angiotensin AT1 receptor blocker losartan, the angiotensin converting enzyme inhibitor captopril, and the direct renin inhibitor aliskiren. Although much of the pathology had declined by 3 weeks, the changes persisted at 6 weeks. The purpose of the study was to extends the time course investigation to 10 weeks and to examines whether the fat embolism effects continue to be blocked by losartan when given at a late time period...
January 13, 2019: Experimental Lung Research
Shi Liming, Li Guixia, Shi Wenxin, Tian Guirong
PURPOSE OF THE STUDY: Pulmonary infection with Klebsiella pneumoniae (KP) and carbapenem-resistant Klebsiella pneumoniae (CRKP) significantly contribute to morbidity and mortality in pneumonia. Recent studies have indicated that High-Mobility Group Box 1 Protein (HMGB1) plays an important role in the prevention and treatment of pneumonia. However the role of HMGB1 in CRKP-induced pneumonia has not been addressed. Materials andMethods: In vivo, we successfully established the KP and CRKP-induced pneumonia mouse model...
December 29, 2018: Experimental Lung Research
Sunan Liu, Pan Zhang, Yanan Liu, Xiaoyan Gao, Juan Hua, Wei Li
PURPOSE: Klebsiella pneumoniae-caused pneumonia is a risk factor for development of lung injury. However, the current clinical isolates of K. pneumoniae are mostly multidrug-resistance and thus must be addressed with new treatments. One ideal approach is to enhance the innate immunity of the infected host through metabolic modulators. MATERIALS AND METHODS: We used GC/MS-based metabolomics to profile the metabolomes among Control, Dead and Survival groups. The key metabolites were administrated in mice, and the bacterial loads in lung and survival were measured...
December 4, 2018: Experimental Lung Research
Cao Bin, He Xiaofeng, Xu Wanzi
PURPOSE: To investigate the effect of microRNA-129 on the migration and invasion in (Non small cell lung cancer) NSCLC cells and involved mechanism. MATERIALS AND METHODS: A549 cells were cultured and transfected with microRNA-129 (miR-129) mimic. Wound-healing assay and transwell assay were used to analyses the migration and invasion of A549 cells, respectively. The SOX4 expression changes were detected by qRT-PCR and Western blot. Dual-luciferase reporter assay was used to determine the binding site between miR-129 and the 3'-UTR of SOX4...
November 26, 2018: Experimental Lung Research
Xue Tian, Guogang Xie, Fengming Ding, Xin Zhou
AIM OF THE STUDY: Matrix metalloproteinases (MMPs) play a critical role in chronic obstructive pulmonary disease (COPD). This study investigated the role of mitogen-activated protein kinases (MAPKs) in MMP-9 secretion of BEAS-2B cells, a human bronchial epithelial cell line and U937 cells, a human myeloid leukaemia cell line, which could differentiate into macrophage, after LPS stimulation, and some details of involved signaling. MATERIALS AND METHODS: MTT assay was used to measure cell viability...
November 23, 2018: Experimental Lung Research
Anette Kocbach Bølling, Tonje Berg Steensen, Neil E Alexis, Liv Ingunn Bjoner Sikkeland
PURPOSE: This paper aimed to test whether induced sputum samples acquired from human volunteers could be used to isolate and culture airway macrophages for in vitro exposures. This was assessed in terms of the culturing success rate, culture purity, viability and responsiveness of cultured cells. MATERIALS AND METHODS: The isolation and culturing procedure was performed over three days. On Day 1, induced sputum samples were obtained, processed and seeded in culture wells...
November 22, 2018: Experimental Lung Research
Sade M B Finn, Uwe Scheuermann, Zoie E Holzknecht, William Parker, Joshua A Granek, Shu S Lin, Erin A McKenney, Andrew S Barbas
AIM OF THE STUDY: The pulmonary microbiota is important for both normal homeostasis and the progression of disease, and may be affected by aspiration of gastric fluid. The aim of this study was to investigate changes in the lung microbiota induced by aspiration of gastric fluid in a laboratory rat model. MATERIAL AND METHODS: Using the intratracheal application method, male rats received aspiration with 0.9% normal saline (n = 11); gastric fluid (n = 24) or sterilized (gamma-irradiated) gastric fluid (n = 12) once-weekly for four weeks...
November 22, 2018: Experimental Lung Research
Bo Liu, Yanqi Wu, Yahui Wang, Yumei Cheng, Ling Yao, Yuqin Liu, Hong Qian, Huilin Yang, Feng Shen
Purpose/aim: Activated coagulation and reduced fibrinolysis in alveolar compartment are an important characteristics in acute respiratory distress syndrome (ARDS). Alveolar epithelial cell type II (AECII) participates in regulating the intra-alveolar abnormalities of coagulation and fibrinolysis mainly through adjusting the productions of tissue factor (TF), plasminogen activator inhibitor (PAI)-1 and activated protein C (APC) in ARDS. NF-κB signal pathway may be involved in coagulation regulation in sepsis-induced ALI...
November 19, 2018: Experimental Lung Research
Liuzhao Cao, Fen Liu, Yahui Liu, Tian Liu, Jinxiang Wu, Jiping Zhao, Junfei Wang, Shuo Li, Jiawei Xu, Liang Dong
PURPOSE: Thymic stromal lymphopoietin (TSLP) acts as a critical cytokine involved in asthmatic airway remodeling. Our study aimed to characterize the crosstalk between airway epithelial cells and fibroblasts regulated by TSLP through the signaling pathways of Mitogen-activated protein kinase (MAPK) and signal transducer and activator of transcription 3 (STAT3). MATERIALS AND METHODS: Human biopsy specimens and lung tissues from mice were stained with hematoxylin and eosin (H&E) and immunohistochemistry...
November 14, 2018: Experimental Lung Research
Marian S Boshra, Ahmed G Almeldien, Randa Salah Eldin, Ahmed A Elberry, Nada Sayed Abdelwahab, Mohamed Nabil Salem, Hoda Rabea, Mohamed E A Abdelrahim
The aim of the present study was to compare the performance of two different dry powder inhalers (DPIs) at different inhalations volumes and inhalation flows. Ventolin Diskus contain blisters of 200µg salbutamol. To test the TED from Aerolizer, salbutamol in Diskus blister was emptied and placed in size 3 capsules suitable for use with Aerolizer. Total emitted dose (TED) delivered by Diskus and Aerolizer was determined using DPI sampling apparatus after one and two inhalations from the same dose. 10-60L/min inhalation flows at 2 and 4L inhalation volume were used in the determination...
October 22, 2018: Experimental Lung Research
Christa Watson-Wright, Priscila Queiroz, Sylvia Rodrigues, Thomas C Donaghey, Joseph D Brain, Ramon M Molina
AIM OF STUDY: Metal contaminants contribute to adverse human health effects via acute and chronic exposures. Acute metal exposures followed by prolonged secondary metal exposures may elicit exaggerated inflammatory responses in certain individuals. The aim of this study is to determine whether repeated pulmonary exposures to zinc chloride (ZnCl2 ) alter subsequent responses to zinc or cerium exposures. MATERIALS AND METHODS: Rats were intratracheally (IT) instilled with physiologic saline (n = 24) or 0...
October 8, 2018: Experimental Lung Research
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