Vera Nickl, Jonathan Fakler, David Ziebolz, Charlotte Rumpel, Linus Stabenow, Johanna Bernhagen, Eva Rampeltshammer, Ralf-Ingo Ernestus, Mario Löhr, Isabel Gugel, Cordula Matthies, Camelia M Monoranu, Carsten Hagemann, Maria Breun
BACKGROUND: To develop a 3D tumor slice model, replicating the individual tumor microenvironment and for individual pharmaceutical testing in vestibular schwannomas with and without relation to NF2. METHODS: Tissue samples from 16 VS patients (14 sporadic, 2 NF2-related) were prospectively analyzed. Slices of 350µm thickness were cultured in vitro, and the 3D tumor slice model underwent thorough evaluation for culturing time, microenvironment characteristics, morphology, apoptosis, and proliferation rates...
February 20, 2024: Journal of Neuroscience Methods