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Current Microbiology

Ugur Azizoglu
Bacillus thuringiensis (Bt) is a gram-positive spore-forming soil microorganism. Because the insecticidal activities of Bt are well known, it has been used as a tool for insect pest control worldwide. The beneficial features of Bt are not limited to its role as an insecticide; it is also used to control phytopathogenic fungi via chitinolytic activity. Bt-related studies are mostly focused on its biocontrol properties. However, studies focusing on the biostimulation and biofertilizer features of Bt, including its interactions with plants, are limited...
May 17, 2019: Current Microbiology
Tanvi Singh, Dileep K Singh
Lindane is an organochlorine pesticide that is highly persistent in the environment. The amassing of lindane has been identified worldwide and has been found to be very toxic to the environment, human, and animal health. Therefore, urgent consideration and management of the problem is necessary. The current study intends to isolate and identify lindane degrading rhizospheric bacteria from Phragmites karka and to study its degradation kinetics. Also, plant growth promoting potential of the bacterium was evaluated in the presence and absence of studied pesticide...
May 15, 2019: Current Microbiology
Dusit Athinuwat, Siraprapa Brooks
The goal of this study was to elucidate the role of the outer membrane protein A (ompA) gene of Xanthomonas axonopodis pv. glycines in bacterial pustule pathogenesis of soybean. An ompA mutant of X. axonopodis pv. glycines KU-P-SW005 was shown to significantly decrease cellulase, pectate lyase, and polysaccharide production. The production of these proteins in the ompA mutant was approximately five times lower than that of the wildtype. The ompA mutant also exhibited modified biofilm development. More importantly, the mutant reduced disease severity to the soybean...
May 14, 2019: Current Microbiology
Wei Xiang, Shaowen Chen, Daizhi Tian, Chunhua Huang, Tiexiang Gao
A rod-shaped, Gram-negative and aerobic bacterium, strain XWS2, was isolated from rhizosphere soil of a camphor tree in Hubei University of Chinese Medicine Huangjiahu Campus, Wuhan, China. Cells grew at 4-37 °C (optimum 28 °C), pH 5.0-9.0 (optimum 7.0) and with 0-5% NaCl (optimum 1%). Colonies growing on tryptone soybean agar are round, beige in color and approximately 2 mm in diameter after 24 h incubation at 28 °C. Pellicle formation during liquid culture and strong fluorescent pigment production on King's B medium are typical features of strain XWS2...
May 11, 2019: Current Microbiology
Yeqing Jin, Hangfei Zhu, Si Luo, Wenwu Yang, Li Zhang, Shanshan Li, Qing Jin, Qin Cao, Shurong Sun, Ming Xiao
Bacillus velezensis strain S3-1 has a broad range of hosts and is used as a biocontrol agent and biofertilizer. However, the interaction of maize root exudates and colonization of the strain S3-1 has not yet been investigated. In our study, strain S3-1 effectively colonized both rhizosphere soil and root tissue. Collected maize root exudates significantly induced the chemotaxis, cluster movement, and biofilm formation of strain S3-1, showing increases of 1.43, 1.6, and 2.08 times, respectively, compared with the control...
May 9, 2019: Current Microbiology
Nihan Unubol, Inci Tuney Kizilkaya, Sinem Oktem Okullu, Kaya Koksalan, Tanil Kocagoz
Several species of mycobacteria cause infections in humans. Species identification of clinical isolates of mycobacteria is very important for the decision of treatment and in choosing the appropriate treatment regimen. We have developed a multiplex PCR method that can identify practically all known species of mycobacteria, by determination of single-nucleotide differences at a total of 13 different polymorphic regions in the genes of rRNA and hsp65, in four PCR mixes. To achieve this goal, single-nucleotide differences in these polymorphic regions were used to divide mycobacterial species into two groups, than four, eight, etc...
May 9, 2019: Current Microbiology
Jeffrey D Palumbo, Teresa L O'Keeffe, B Joy Quejarro, Alan Yu, Alison Zhao
Species belonging to Aspergillus section Nigri are widespread in the vineyard environment, both in soil and on plant surfaces. We used plate counts and droplet digital PCR (ddPCR) methods to compare populations of the four most prevalent species (A. carbonarius, A. niger, A. welwitschiae, and A. tubingensis) over two consecutive years in conventional and organic vineyards, to determine whether management affects the potential distribution of mycotoxigenic Aspergillus species. In 2016, plate counts showed that soil populations of total filamentous fungi and of Aspergillus section Nigri species were not significantly different between conventional and organic vineyards...
May 9, 2019: Current Microbiology
Xiang Li, Yan Sun, Junjie Liu, Qin Yao, Guanghua Wang
Although bacteriophages are the most abundant biological entities on the planet, their genetic diversity, especially in natural wetlands, is poorly understood. In this study, the genetic diversity of cyanopodoviruses in sediments of two coastal wetlands in Northeast China was investigated by targeting the DNA polymerase (pol) gene. A total of 66 DNA pol clones were obtained. A BLAST search at the amino acid level showed that the obtained sequences had the highest identity ranged from 83 to 99% to the known sequences...
May 8, 2019: Current Microbiology
Gyana Prakash Mahapatra, Surabhi Raman, Suman Nayak, Sushanto Gouda, Gitishree Das, Jayanta Kumar Patra
Marine actinomycetes are prolific sources of marine drug discovery system contributing for several bioactive compounds of biomedical prominence. Metagenomics, a culture-independent technique through its sequence- and function-based screening has led to the discovery and synthesis of numerous biologically significant compounds like polyketide synthase, Non-ribosomal peptide synthetase, antibiotics, and biocatalyst. While metagenomics offers different advantages over conventional sequencing techniques, they also have certain limitations including bias classification, non-availability of quality DNA samples, heterologous expression, and host selection...
May 8, 2019: Current Microbiology
Nancy Castellanos, Jun Nakanouchi, Dennis Irfan Yüzen, Sammie Fung, Jennifer S Fernandez, Claudia Barberis, Lorena Tuchscherr, Maria Soledad Ramirez
Diabetic foot ulcer infections are frequently polymicrobial in nature and exhibit increased morbidity and mortality, as well as, treatment failures. Interactions between Acinetobacter baumannii and Staphylococcus aureus were studied, which showed strain-dependent changes in growth and antibiotic susceptibility. This study examined the interactions between two clinical strains of A. baumannii (1929) and S. aureus (1928) that were recovered from skin and soft tissues of a diabetic patient. When S. aureus 1928 and A...
May 3, 2019: Current Microbiology
Yun Kyong Lim, Soon-Nang Park, Jeong Hwan Shin, Young-Hyo Chang, Yeseul Shin, Jayoung Paek, Hongik Kim, Joong-Ki Kook
A novel facultative anaerobic and Gram-stain-positive coccus, designated strain ChDC F135T , was isolated from human subgingival dental plaque of periodontitis lesion and was characterized by polyphasic taxonomic analysis. The 16S rRNA gene (16S rDNA) sequence of strain ChDC F135T was closest to that of Streptococcus sinensis HKU4T (98.2%), followed by Streptococcus intermedia SK54T (97.0%), Streptococcus constellatus NCTC11325T (96.0%), and Streptococcus anginosus NCTC 10713T (95.7%). In contrast, phylogenetic analysis based on the superoxide dismutase gene (sodA) and the RNA polymerase beta-subunit gene (rpoB) showed that the nucleotide sequence similarities of strain ChDC F135T were highly similar to the corresponding genes of S...
May 3, 2019: Current Microbiology
Jing He, Le Hai, Khongorzul Orgoldol, Li Yi, Liang Ming, Fucheng Guo, Guowei Li, Rimutu Ji
The complex gut microbiota plays a key role in host metabolism and health. However, the core microbial communities in the different aged Bactrian camels remain totally unclear. We used high-throughput 16S rRNA gene sequencing to examine the temporal variability of the fecal microbiota in Bactrian camels. At 2 months of age, the fecal microbiota was composed of Firmicutes, Proteobacteria, and Actinobacteria. At 1 and 3 years of age, the fecal microbiota was dominated by Firmicutes, Bacteroidetes, and Verrucomicrobia...
April 27, 2019: Current Microbiology
Louis Berrios, Bert Ely
The original version of this article unfortunately contained mistakes in Table 1 values. Some of the values in "TAY-ASD who received services" were incorrect. The corrected Table 1 is given below.
April 27, 2019: Current Microbiology
Soon-Nang Park, Yun Kyong Lim, Jeong Hwan Shin, Young-Hyo Chang, Yeseul Shin, Jayoung Paek, Hongik Kim, Joong-Ki Kook
A novel facultative anaerobic, Gram-stain-negative coccus, designated strain ChDC B345T , was isolated from human pericoronitis lesion and was characterized by polyphasic taxonomic analysis. The 16S ribosomal RNA gene (16S rDNA) sequence revealed that the strain belonged to the genus Streptococcus. The 16S rDNA sequence of strain ChDC B345T was most closely related to those of  Streptococcus mitis NCTC 12261T (99.5%) and Streptococcus pseudopneumoniae ATCC BAA-960T (99.5%). Complete genome of strain ChDC B345T was 1,972,471 bp in length and the G + C content was 40...
April 26, 2019: Current Microbiology
Guiding Li, Xiu Chen, Youlong Li, Songbiao Shi, Longqian Jiang, Yi Jiang, Li Han, Xueshi Huang, Chenglin Jiang
Strains YIM 102796T and YIM 102701-2T were isolated from the feces of Macaca mulatta and Hylobates hoolock, respectively, living in the Yunnan Wild Animal Park, Yunnan province of China. The two strains were Gram-stain-negative, non-gliding, produced flexirubin pigments, non-flagellated and aerobic bacteria. The 16S rRNA gene-based phylogenetic analysis indicate that both YIM 102796T and YIM 102701-2T are members of the genus Flavobacterium, closely related to F. ummariense DS-12T (95.9% similarity) and F. ceti 454-2T (93...
April 26, 2019: Current Microbiology
Burak Alaylar, Medine Güllüce, Mehmet Karadayi, Mine Isaoglu
In this study, the newly designed pqq gene-specific primer sets were used for determination of phosphate-solubilizing capabilities of bacterial isolates from the agricultural regions of Erzurum. The specificity of newly designed primer sets (PqqA2F/PqqA2R, Pqq5F/Pqq5R, PqqF2/PqqF2R) were tested against ten isolates, whose phosphate-solubilizing activities were initially proved by the conventional methods. Non-phosphate-solubilizing bacteria were also chosen as negative control. According to the results, five of ten phosphate-solubilizing bacteria with PqqA2F/PqqA2R, two of ten phosphate-solubilizing bacteria with Pqq5F/Pqq5R primer set, and one of ten phosphate solubilizing with PqqF2F/PqqF2R bacteria were successfully amplificated in the PCR assay and none of the non-phosphate-solubilizing bacteria was amplificated...
April 25, 2019: Current Microbiology
Zhihao Tu, Sayumi Yamada, Dagula Hu, Yoshitada Ito, Tomohito Iwasaki, Akihiro Yamaguchi
An edible gall is formed between the third and fourth nodes beneath the apical meristem near the base of Zizania latifolia shoots. This gall is harbored by and interacts with the smut fungus Ustilago esculenta. The gall is also a valuable vegetable called "white bamboo," jiaobai or gausun in China and makomotake in Japan. Five samples of the galls harvested at different stages of swelling were used to isolate microorganisms by culturing. Isolated fungal and bacterial colonies were identified by DNA sequencing and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry, respectively...
April 24, 2019: Current Microbiology
Yuping Xiang, Yongmin Wang, Hong Shen, Dingyong Wang
Mercury (Hg) methylation is mainly a microbial process mediated by anaerobes. The continued study of Pseudomonas putida (P. putida) strain TGRB4 genome was inspired by the fact that it can transform Hg into the highly toxic methylmercury (MeHg) under aerobic conditions. P. putida strain TGRB4 is a Gram-negative rod-shaped Gamma-proteobacterium (γ-proteobacterium), isolated from the soil in a typical water level fluctuation zone (WLFZ) of the Three Gorges Reservoir (TGR), which suffered from seasonally water level alternations every year...
April 19, 2019: Current Microbiology
Gyeongtaek Gong, Hyun Ju Oh, Sukhyeong Cho, Seil Kim, Min-Kyu Oh, Youngsoon Um, Sun-Mi Lee
Several bioprocessing technologies, such as separate hydrolysis and fermentation (SHF), simultaneous saccharification and fermentation (SSF), and consolidated bioprocessing (CBP), have been highlighted to produce bio-based fuels and chemicals from lignocellulosic biomass. Successful CBP, an efficient and economical lignocellulosic biorefinery process compared with other processes, requires microorganisms with sufficient cellulolytic activity and biofuel/chemical-producing ability. Here, we report the complete genome of Paenibacillus sp...
April 16, 2019: Current Microbiology
Armaghan Soltani Shirazi, Morvarid Shafiei, Hamid Solgi, Mohammad Mehdi Aslani, Omid Azizi, Farzad Badmasti
Successful clones of Acinetobacter baumannii cause a variety of nosocomial infections through serum resistance, biofilm formation, and antimicrobial resistance as virulence capabilities. Fifty clinical isolates of multidrug-resistant (MDR) A. baumannii were analyzed for clonal relatedness, serum resistance, biofilm formation, and in vivo assays. Furthermore, some virulence genes, sequence variation of ompA, and its expression were studied. The MLST (multilocus sequence typing) results showed that there were three sequence types among MDR isolates including ST2 (64%, 32/50), ST513 (30%, 15/50), and ST1 (6%, 3/50)...
April 15, 2019: Current Microbiology
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