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Pathology, Research and Practice

Farres Obeidin, Lawrence J Jennings, Borislav A Alexiev
Sinonasal glomangiopericytoma (SNGP) is a neoplasm arising in the nasal cavity and paranasal sinuses that shows perivascular myoid differentiation. The diagnosis of SNGP may be diagnostically challenging due to a large number of potential mimics. In the present study, we sought to characterize the histological and molecular features of six cases of SNGP found in prior surgical pathology records over a 15-year period. The average age at diagnosis was 48.5 years (range: 31-78 years), and the male-to-female ratio was 1:1...
February 3, 2019: Pathology, Research and Practice
Rocco Cappellesso, Marcello Lo Mele, Giada Munari, Erik Rosa-Rizzotto, Ennio Guido, Franca De Lazzari, Pierluigi Pilati, Marco Tonello, Fabio Farinati, Stefano Realdon, Matteo Fassan, Massimo Rugge
Colorectal cancer (CRC) is a heterogeneous group of diseases both from the morphological and molecular point of view. The sessile serrated adenoma/polyp (SSA/P) has been proposed as the precursor lesion of CRCs characterized by CpG island methylator phenotype (CIMP), DNA mismatch repair (MMR) system deficiency, and BRAF gene mutations. However, no study so far investigated the molecular landscape of "sessile serrated" adenoma to carcinoma transition in early CRCs. Six formalin-fixed paraffin-embedded CRCs developed within SSA/P were profiled for the immunohistochemical expression of MMR proteins (MLH1, MSH2, MSH6, PMS2, and Ep-CAM), p16, and β-catenin...
February 3, 2019: Pathology, Research and Practice
Nahed A Soliman, Shaimaa M Yussif, Abdelhadi M Shebl
INTRODUCTION: Syndecan-1 is heparan sulfate proteoglycans (HSPGs) that is used as coreceptors for signaling of growth factors. The comprehensive effect of syndecan-1 is to augment receptor stimulation at little ligand concentrations. THE GOAL OF THIS RESEARCH: is to study syndecan-1 expression in breast carcinoma and its value in predicting the prognosis in comparison to other clinicopathological parameters. MATERIAL &METHODS: immunohistochemistry study for syndecan-1 is done on 103 cases of invasive breast carcinoma...
February 2, 2019: Pathology, Research and Practice
Dan Zhang, Ying Wu, Chong Zhang, Wenzhu Zhang, Jun Zou, Gengru Jiang
BACKGROUND: The identification of the M-type phospholipase A2 receptor (PLA2R) is a breakthrough recognized as a major target for adults with idiopathic membranous nephropathy (IMN). However, the role PLA2R played in pediatric patients with IMN, particularly in Chinese, has yet to be determined. METHODS: This retrospective study included 187 adult patients and 38 pediatric patients aged 17 years or younger with biopsy proved IMN. The pediatric cohort consisted of 27 children aged from 1 to 12 years and 11 children aged from 13 to 17...
February 1, 2019: Pathology, Research and Practice
Thomas Albrecht, Alexander Harms, Stephanie Roessler, Benjamin Goeppert
NUT carcinoma is a rarely diagnosed, poorly differentiated subtype of squamous cell carcinoma, defined by chromosomal rearrangements of the gene encoding nuclear protein of the testis (NUT). It is characterized by extremely aggressive clinical behavior resulting in a dismal prognosis, with a median survival of 6.7 months. Though most frequently detected along the body midline, NUT carcinoma can arise in any organ. Fewer than 100 cases have been reported in medical literature with the majority of patients being children or young adults...
January 30, 2019: Pathology, Research and Practice
Guowei Li, Yue Zhu
Chemoresistance is a major obstacle in treating cancer, including osteosarcoma. LncRNA ANRIL (ANRIL) is involved in the growth and metastasis of osteosarcoma cells, however, its role in chemoresistance remains unclear. In this study, ANRIL shRNA was used to knock down its endogenous expression in U2-OS and Saos-2 osteosarcoma cell lines. Our data showed that ANRIL-silenced cells were more sensitive to cisplatin: apoptotic ratio was increased and cleaved caspase-3 level was upregulated. Furthermore, the expression level of miR-125a-5p, a microRNA that can bind to ANRIL, was elevated in ANRIL-silenced cells...
January 30, 2019: Pathology, Research and Practice
Qian Lyu, Li Jin, Xiaobin Yang, Fang Zhang
Oral squamous cell carcinoma (OSCC) is a common type of malignant oral cancer with high recurrence. MYC-induced long non-coding RNA (MINCR) has been reported as a tumor suppressor in liver cancer and lung cancer. Whereas, it is unknown whether MINCR exerted function in OSCC progression. This study focused on its function and mechanism in OSCC. At first, the expression level of MINCR in OSCC tissues and cell lines as well as corresponding normal controls was evaluated by qRT-PCR assay. The relative high level of MINCR was observed in OSCC tissues and cell lines...
January 30, 2019: Pathology, Research and Practice
Yujie Dong, Zhen Zhou, Jianguo Wang, Li Ma, Zichen Liu, Yuxuan Wang, Jing Song, Shucai Zhang, Nanying Che
OBJECTIVE: Recently, a low frequency of de novo T790M mutations existing in tumor tissues before TKIs therapy has been reported. However, the origin of T790M and its impact on clinical outcomes is still being debated. This study aimed to use highly sensitive methods to detect T790M before and after TKIs therapy and investigated the correlation of T790M with clinical prognosis. PATIENTS AND METHODS: Matched tumor samples before and after treatment were collected from 61 lung adenocarcinoma (LAC) patients in Beijing Chest Hospital between June 2014 to October 2017...
January 30, 2019: Pathology, Research and Practice
Christian D Lütken, Anne-Marie K Fiehn, Birgitte Federspiel, Michael P Achiam
BACKGROUND/INTRODUCTION: Isolated tumor cells (ITC) are tumor cells identified in the regional lymph nodes of patients with adeno- or squamous cell carcinoma of the esophagogastric junction (EGJ) or the esophagus. The current staging guidelines for these cancers do not assign any prognostic relevance to ITC, but their role remains debatable. We evaluated current literature to provide an overview of the prognostic relevance of ITC in regional lymph nodes of patients diagnosed with node negative cancer of the esophagus and EGJ...
January 30, 2019: Pathology, Research and Practice
Bin Yu, Youming Ding, Xiaofeng Liao, Changhua Wang, Bin Wang, Xiaoyan Chen
BACKGROUND: TONSL has been suggested to function as an oncogene in lung, esophageal and cervical cancer. This study was aimed to identify the expression of TONSL and its role in hepatocellular carcinoma (HCC). METHODS: By data mining in the Cancer Genome Atlas (TCGA) and Human Protein Atlas (HPA) databases, the expression profile of TONSL, its clinical significance, the potential mechanisms of its dysregulation and its underlying biological function in HCC were investigated...
January 30, 2019: Pathology, Research and Practice
Quang Ngoc Nguyen, Linh Dieu Vuong, Van-Long Truong, To Van Ta, Nam Trung Nguyen, Hung Phi Nguyen, Ha Hoang Chu
Genetic and epigenetic alterations importantly contribute to the pathogenesis of lung cancer. In the study, we measured the frequency and distribution of molecular abnormalities of EGFR as well as the aberrant promoter methylations of BRCA1, MGMT, MLH1, and RASSF1A in Vietnamese lung adenocarcinomas. We investigated the association between genetic and epigenetic alteration, and between each abnormality with clinicopathologic parameters. Somatic EGFR mutation that was found in 49/139 (35.3%) lung adenocarcinomas showed a significant association with young age, female gender, and non-smokers...
January 29, 2019: Pathology, Research and Practice
Qiuhui Hu, Sheng Tai, Jicai Wang
OBJECTIVES: Long non-coding RNAs (lncRNAs) are a group of noncoding RNAs with length larger than 200 nucleotides. LncRNAs have limited or no protein-coding capacity because of lack of obvious open reading frame. An increasing number of researches have shown that lncRNAs participate in the complex regulation network of cancer and play an important role in tumourigenesis and progression such as proliferation, migration and invasion. LncRNA FOXD2 adjacent opposite strand RNA 1 (FOXD2-AS1), located on chromosome 1p33 and with a transcript length of 2527 nucleotides, is a novel cancer-related lncRNA...
January 29, 2019: Pathology, Research and Practice
Mi Jung Kwon, Jae Seung Soh, Sang-Woo Lim, Ho Suk Kang, Hyun Lim
Human epidermal growth factor 2 (HER2) is a candidate therapeutic and prognostic marker for rectal cancer treated with neoadjuvant chemoradiotherapy. The specific frequency and prognostic role of HER2 protein expression and HER2 gene amplification in those rectal cancers has not been fully investigated. Pretreatment biopsied and surgically resected formalin-fixed paraffin-embedded tissues from 74 patients were retrospectively evaluated for HER2 protein expression and HER2 gene copy number using immunohistochemistry (IHC) and silver in situ hybridization (SISH), respectively...
January 28, 2019: Pathology, Research and Practice
Qiuyan Xia, Yan Zhou, Hongmei Yong, Xudong Wang, Wei Zhao, Guipeng Ding, Jin Zhu, Xiaohua Li, Zhenqing Feng, Bing Wang
Epiregulin (EREG) is a novel family member of EGF-like ligands and have elevated expression in a variety of human cancers. EREG expression promotes tumor progression and metastasis and reduces patient survival. However, the expression of EREG and its prognostic value are not clear in gastric cancer (GC). We assessed EREG mRNA and protein expression in GC tissues from Chinese patients using quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemical staining of tissue microarray, and analyzed the correlation between the level of EREG expression and patient clinical characteristics and prognosis...
January 28, 2019: Pathology, Research and Practice
Xiaomeng Jiang, Menglin Jiang, Min Xu, Jing Xu, Yi Li
BACKGROUND: Gastric cancer (GC) is one of the most common cancers globally leading to 850,000 deaths each year. GC patients are often diagnosed at advanced stages which results in poor prognosis. This study aimed to identify a novel circulating miRNA as the diagnostic biomarker of GC and further explore its regulatory mechanisms in GC. MATERIALS AND METHODS: First, the candidate serum miRNA was selected after analysis of microarray data. Then, the levels of candidate miRNA in the serum of GC patients were validated in an independent cohort...
January 28, 2019: Pathology, Research and Practice
Xiaolan Huang, Liping She, Xiangmin Luo, Shuzhen Huang, Jinxiang Wu
Polycystic ovary syndrome (PCOS) is one of the most complex and common reproductive and endocrinologic disorders in the child-bearing age of women. Recently, miR-222 were reported to be associated with the etiology of PCOS. However, the function of miR-222 during the pathogenesis of PCOS remains unclear. In the present study, we aimed to investigate the role of miR-222 in PCOS. Firstly, miR-222 expression was examined by quantitative real-time PCR (qRT-PCR) in PCOS. The effects of miR-222 on proliferation, apoptosis and cell cycle in KGN cells were analyzed by CCK-8 assay and flow cytometry analysis, respectively...
January 28, 2019: Pathology, Research and Practice
Cheng Zhang, Yu Liang, Ming-Hui Ma, Kun-Zhe Wu, Dong-Qiu Dai
AIM: The present study aims to identify aberrantly methylated and differentially expressed genes (DEGs) in gastric cancer (GC) and explore their potential role in the carcinogenesis and development of GC. METHODS: The original RNA-Seq, clinical information and Illumina Human Methylation 27 Chip data associated with GC were downloaded from The Cancer Genome Atlas (TCGA) database using the gdc-client tool. The DEGs and aberrantly methylated genes (AMGs) were screened with edgeR and limma package in R, respectively...
January 28, 2019: Pathology, Research and Practice
C Díaz Del Arco, D García, C Sanabria, E Rodríguez Escudero, I Domínguez, G Sanz Ortega, M J Fernández Aceñero
INTRODUCTION: Anal cytology (AC) can be used as a screening tool for detection of anal HPV associated lesions, mainly in men who have sex with men and in immunosuppressed patients. Our aim is to review our experience with AC in women. MATERIAL & METHODS: We have retrospectively reviewed all AC diagnosed between 2010-2017 in a single tertiary hospital (n = 644) and selected those performed in women (n = 158). RESULTS: 24.53% of AC were performed in women...
January 28, 2019: Pathology, Research and Practice
Dongyi Zhu, Li Gu, Zhanxia Li, Wenjing Jin, Qingchun Lu, Tao Ren
BACKGROUND: MiR-138-5p is regarded as a tumour suppressor in many cancers. Transforming growth factor beta (TGF-β) often acts as a tumor promotor at the late stages of human cancers. However, the function of miR-138-5p on lung adenocarcinoma cells induced by TGF-β remains to be further confirmed. METHODS: RT-qPCR was used to detect the expression of human lung adenocarcinoma tissues, adjacent normal tissues, and relative cell lines. When the lung adenocarcinoma cells A549 and H1299 were transfected with negative control (NC), miR-138-5p mimics and miR-138-5p inhibitor by lipofectamine3000 and treated with or without TGF-β1, the lung adenocarcinoma cell function was detected by Immunofluorescence, Western blotting (WB), cell counting Kit-8 (CCK8), colony formation, EdU, Wound-healing and Transwell assays...
January 28, 2019: Pathology, Research and Practice
Priya E Skaria, Atif A Ahmed, Hong Yin, Kathleen Nicol, Kimberly J Reid, Vivekanand Singh
Papillary thyroid carcinoma (PTC) is the most common differentiated thyroid cancer in children; and the follicular variant is the second most common variant after the classic subtype. The histological appearance of follicular variant of papillary thyroid cancer (FVPTC), can be mimicked by benign follicular nodules. Pediatric pathologists encountering such lesions with FVPTC-like appearance may err on diagnosing the benign lesions as malignant. In adult patients, several immunohistochemical markers have emerged recently as a useful adjunct to distinguish differentiated thyroid carcinomas from benign follicular lesions...
January 28, 2019: Pathology, Research and Practice
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